Dynamic changes of apoptosis and expression of Bcl-2 family members in the posthatch hippocampus of Bengalese finches
The hippocampus of songbirds plays an important role in spatial memory, and probably in song learning. Although prolonged neuronal generation and apoptosis are thought to be closely correlated with memory function, natural changes of the number of neurons and in apoptosis in the hippocampus of songb...
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| Veröffentlicht in: | Brain research 2006-08, Vol.1107 (1), p.58-69 |
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| creator | Zeng, Lei Lu, Xiaohua Zeng, Shaoju Lin, Yutao Sun, Yingyu Zhang, Xinwen Zuo, Mingxue |
| description | The hippocampus of songbirds plays an important role in spatial memory, and probably in song learning. Although prolonged neuronal generation and apoptosis are thought to be closely correlated with memory function, natural changes of the number of neurons and in apoptosis in the hippocampus of songbirds have not been fully investigated during development and in the adult. In the current study, we examined developmental changes in the volume and the number of neurons and apoptotic cells in the hippocampus of songbirds (
Lonchura striata) from posthatch day (P5) to adulthood. Apoptotic cells were determined by Nissl staining and immunohistochemistry for cleaved caspase-3, a key apoptotic caspase executioner. The expression levels of Bcl-2 family member mRNA and protein, including Bcl-2, Bcl-x
L and Bax, were also investigated. Our results indicated that: (1) the hippocampus volume significantly increased from P5 to P60, although the number of neurons remained stable in all studied stages; (2) the number of apoptotic cells was highest at P45, based either on the Nissl staining or on the immunohistochemistry for caspase-3; (3) Bcl-2 mRNA expression was high from P5 to adulthood, while Bax mRNA declined abruptly from P5 to adulthood, and Bcl-x mRNA was high after P45. Bcl-2 protein was only detected at P5 and P15, while detection of Bcl-x
L and Bax proteins paralleled levels of mRNA expression. Our study provides detailed changes of apoptosis in the posthatch songbird hippocampus, suggesting an important role for caspase-3 and Bcl-2 family members in hippocampus apoptosis. |
| doi_str_mv | 10.1016/j.brainres.2006.05.085 |
| format | Article |
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Lonchura striata) from posthatch day (P5) to adulthood. Apoptotic cells were determined by Nissl staining and immunohistochemistry for cleaved caspase-3, a key apoptotic caspase executioner. The expression levels of Bcl-2 family member mRNA and protein, including Bcl-2, Bcl-x
L and Bax, were also investigated. Our results indicated that: (1) the hippocampus volume significantly increased from P5 to P60, although the number of neurons remained stable in all studied stages; (2) the number of apoptotic cells was highest at P45, based either on the Nissl staining or on the immunohistochemistry for caspase-3; (3) Bcl-2 mRNA expression was high from P5 to adulthood, while Bax mRNA declined abruptly from P5 to adulthood, and Bcl-x mRNA was high after P45. Bcl-2 protein was only detected at P5 and P15, while detection of Bcl-x
L and Bax proteins paralleled levels of mRNA expression. Our study provides detailed changes of apoptosis in the posthatch songbird hippocampus, suggesting an important role for caspase-3 and Bcl-2 family members in hippocampus apoptosis.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2006.05.085</identifier><identifier>PMID: 16842761</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Age Factors ; Analysis of Variance ; Animals ; Animals, Newborn ; Apoptosis ; Apoptosis - physiology ; bcl-2-Associated X Protein - metabolism ; Bengalese finch ; Biological and medical sciences ; Caspase 3 ; Caspases - metabolism ; Cell Count - methods ; Development ; Development. Senescence. Regeneration. Transplantation ; Female ; Finches ; Fundamental and applied biological sciences. Psychology ; Gene Expression - physiology ; Hippocampus - cytology ; Hippocampus - growth & development ; Hippocampus - metabolism ; Immunohistochemistry ; Immunohistochemistry - methods ; In situ hybridization ; In Situ Hybridization - methods ; Lonchura striata ; Male ; Neurons - metabolism ; Nonlinear Dynamics ; Oncoprotein ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 2006-08, Vol.1107 (1), p.58-69</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c374t-4fb568fd4455c50eacc4365138e2ecbbc82fab629bc035298aed4e440902ad6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006899306016313$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18051359$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16842761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Lei</creatorcontrib><creatorcontrib>Lu, Xiaohua</creatorcontrib><creatorcontrib>Zeng, Shaoju</creatorcontrib><creatorcontrib>Lin, Yutao</creatorcontrib><creatorcontrib>Sun, Yingyu</creatorcontrib><creatorcontrib>Zhang, Xinwen</creatorcontrib><creatorcontrib>Zuo, Mingxue</creatorcontrib><title>Dynamic changes of apoptosis and expression of Bcl-2 family members in the posthatch hippocampus of Bengalese finches</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The hippocampus of songbirds plays an important role in spatial memory, and probably in song learning. Although prolonged neuronal generation and apoptosis are thought to be closely correlated with memory function, natural changes of the number of neurons and in apoptosis in the hippocampus of songbirds have not been fully investigated during development and in the adult. In the current study, we examined developmental changes in the volume and the number of neurons and apoptotic cells in the hippocampus of songbirds (
Lonchura striata) from posthatch day (P5) to adulthood. Apoptotic cells were determined by Nissl staining and immunohistochemistry for cleaved caspase-3, a key apoptotic caspase executioner. The expression levels of Bcl-2 family member mRNA and protein, including Bcl-2, Bcl-x
L and Bax, were also investigated. Our results indicated that: (1) the hippocampus volume significantly increased from P5 to P60, although the number of neurons remained stable in all studied stages; (2) the number of apoptotic cells was highest at P45, based either on the Nissl staining or on the immunohistochemistry for caspase-3; (3) Bcl-2 mRNA expression was high from P5 to adulthood, while Bax mRNA declined abruptly from P5 to adulthood, and Bcl-x mRNA was high after P45. Bcl-2 protein was only detected at P5 and P15, while detection of Bcl-x
L and Bax proteins paralleled levels of mRNA expression. Our study provides detailed changes of apoptosis in the posthatch songbird hippocampus, suggesting an important role for caspase-3 and Bcl-2 family members in hippocampus apoptosis.</description><subject>Age Factors</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Apoptosis</subject><subject>Apoptosis - physiology</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Bengalese finch</subject><subject>Biological and medical sciences</subject><subject>Caspase 3</subject><subject>Caspases - metabolism</subject><subject>Cell Count - methods</subject><subject>Development</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Female</subject><subject>Finches</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - physiology</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - growth & development</subject><subject>Hippocampus - metabolism</subject><subject>Immunohistochemistry</subject><subject>Immunohistochemistry - methods</subject><subject>In situ hybridization</subject><subject>In Situ Hybridization - methods</subject><subject>Lonchura striata</subject><subject>Male</subject><subject>Neurons - metabolism</subject><subject>Nonlinear Dynamics</subject><subject>Oncoprotein</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EosvCV6h8gVuC49iOc4OWv1IlLnC2HGfSeJXYwZNU7LfHyy7qsafRaH7vzdiPkOuKlRWr1PtD2SXrQwIsOWOqZLJkWj4ju0o3vFBcsOdkx_Kk0G1bX5FXiIfc1nXLXpKrSmnBG1XtyPbpGOzsHXWjDfeANA7ULnFZI3qkNvQU_ix5C_oYTrMbNxWcDlkyHekMcwcJqQ90HYEuEdfRrm6ko1-W6Oy8bP8MbyDc2wkQ6OCDGwFfkxeDnRDeXOqe_Pry-eftt-Lux9fvtx_vClc3Yi3E0Emlh14IKZ1kYJ0TtZJVrYGD6zqn-WA7xdvOsVryVlvoBQjBWsZtr7p6T96dfZcUf2-Aq5k9OpgmGyBuaJRuZJsdnwSrthZSqCaD6gy6FBETDGZJfrbpaCpmTsmYg_mfjDklY5g0OZksvL5s2LoZ-kfZJYoMvL0AFp2dhmSD8_jIaZYfnq_dkw9nDvLHPXhIBp2H4KD3Cdxq-uifuuUvXeWxiQ</recordid><startdate>20060830</startdate><enddate>20060830</enddate><creator>Zeng, Lei</creator><creator>Lu, Xiaohua</creator><creator>Zeng, Shaoju</creator><creator>Lin, Yutao</creator><creator>Sun, Yingyu</creator><creator>Zhang, Xinwen</creator><creator>Zuo, Mingxue</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20060830</creationdate><title>Dynamic changes of apoptosis and expression of Bcl-2 family members in the posthatch hippocampus of Bengalese finches</title><author>Zeng, Lei ; Lu, Xiaohua ; Zeng, Shaoju ; Lin, Yutao ; Sun, Yingyu ; Zhang, Xinwen ; Zuo, Mingxue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-4fb568fd4455c50eacc4365138e2ecbbc82fab629bc035298aed4e440902ad6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Age Factors</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Apoptosis</topic><topic>Apoptosis - physiology</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Bengalese finch</topic><topic>Biological and medical sciences</topic><topic>Caspase 3</topic><topic>Caspases - metabolism</topic><topic>Cell Count - methods</topic><topic>Development</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Female</topic><topic>Finches</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - physiology</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - growth & development</topic><topic>Hippocampus - metabolism</topic><topic>Immunohistochemistry</topic><topic>Immunohistochemistry - methods</topic><topic>In situ hybridization</topic><topic>In Situ Hybridization - methods</topic><topic>Lonchura striata</topic><topic>Male</topic><topic>Neurons - metabolism</topic><topic>Nonlinear Dynamics</topic><topic>Oncoprotein</topic><topic>Proto-Oncogene Proteins c-bcl-2 - genetics</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Lei</creatorcontrib><creatorcontrib>Lu, Xiaohua</creatorcontrib><creatorcontrib>Zeng, Shaoju</creatorcontrib><creatorcontrib>Lin, Yutao</creatorcontrib><creatorcontrib>Sun, Yingyu</creatorcontrib><creatorcontrib>Zhang, Xinwen</creatorcontrib><creatorcontrib>Zuo, Mingxue</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Lei</au><au>Lu, Xiaohua</au><au>Zeng, Shaoju</au><au>Lin, Yutao</au><au>Sun, Yingyu</au><au>Zhang, Xinwen</au><au>Zuo, Mingxue</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic changes of apoptosis and expression of Bcl-2 family members in the posthatch hippocampus of Bengalese finches</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2006-08-30</date><risdate>2006</risdate><volume>1107</volume><issue>1</issue><spage>58</spage><epage>69</epage><pages>58-69</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The hippocampus of songbirds plays an important role in spatial memory, and probably in song learning. Although prolonged neuronal generation and apoptosis are thought to be closely correlated with memory function, natural changes of the number of neurons and in apoptosis in the hippocampus of songbirds have not been fully investigated during development and in the adult. In the current study, we examined developmental changes in the volume and the number of neurons and apoptotic cells in the hippocampus of songbirds (
Lonchura striata) from posthatch day (P5) to adulthood. Apoptotic cells were determined by Nissl staining and immunohistochemistry for cleaved caspase-3, a key apoptotic caspase executioner. The expression levels of Bcl-2 family member mRNA and protein, including Bcl-2, Bcl-x
L and Bax, were also investigated. Our results indicated that: (1) the hippocampus volume significantly increased from P5 to P60, although the number of neurons remained stable in all studied stages; (2) the number of apoptotic cells was highest at P45, based either on the Nissl staining or on the immunohistochemistry for caspase-3; (3) Bcl-2 mRNA expression was high from P5 to adulthood, while Bax mRNA declined abruptly from P5 to adulthood, and Bcl-x mRNA was high after P45. Bcl-2 protein was only detected at P5 and P15, while detection of Bcl-x
L and Bax proteins paralleled levels of mRNA expression. Our study provides detailed changes of apoptosis in the posthatch songbird hippocampus, suggesting an important role for caspase-3 and Bcl-2 family members in hippocampus apoptosis.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>16842761</pmid><doi>10.1016/j.brainres.2006.05.085</doi><tpages>12</tpages></addata></record> |
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| subjects | Age Factors Analysis of Variance Animals Animals, Newborn Apoptosis Apoptosis - physiology bcl-2-Associated X Protein - metabolism Bengalese finch Biological and medical sciences Caspase 3 Caspases - metabolism Cell Count - methods Development Development. Senescence. Regeneration. Transplantation Female Finches Fundamental and applied biological sciences. Psychology Gene Expression - physiology Hippocampus - cytology Hippocampus - growth & development Hippocampus - metabolism Immunohistochemistry Immunohistochemistry - methods In situ hybridization In Situ Hybridization - methods Lonchura striata Male Neurons - metabolism Nonlinear Dynamics Oncoprotein Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism Vertebrates: nervous system and sense organs |
| title | Dynamic changes of apoptosis and expression of Bcl-2 family members in the posthatch hippocampus of Bengalese finches |
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