3D Structure of Eukaryotic Flagella in a Quiescent State Revealed by Cryo-Electron Tomography

We have used cryo-electron tomography to investigate the 3D structure and macromolecular organization of intact, frozen-hydrated sea urchin sperm flagella in a quiescent state. The tomographic reconstructions provide information at a resolution better than 6 nm about the in situ arrangements of macr...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2005-11, Vol.102 (44), p.15889-15894
Hauptverfasser:	Nicastro, Daniela, McIntosh, J. Richard, Baumeister, Wolfgang
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological Sciences Cell adhesion & migration Cell membranes Cell motility Cellular biology Cryoelectron Microscopy - methods Dyneins - chemistry Echinoidea Electrons Eukaryotic Cells - cytology Flagella Flagella - chemistry Imaging, Three-Dimensional - methods Male Marine Microtubules Microtubules - chemistry Molecular Motor Proteins - chemistry Molecular structure Periodicity Protein Structure, Tertiary Sea Urchins Spermatozoa Spermatozoa - cytology Symmetry Tomography Waveforms
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