The effects of heat on the biological activity of recombinant human bone morphogenetic protein-2

This study was designed to investigate effects of heat on the bone-inducing activity of recombinant human bone morphogenetic protein (rhBMP)-2. rhBMP-2 samples were heated at 50, 70, 90, or 100 degrees C for 15 min, or 1, 2, 4, or 8 h, or autoclaved at 120 degrees C for 15 min. The bone-inducing act...

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Veröffentlicht in:	Journal of bone and mineral metabolism 2005-11, Vol.23 (6), p.420-425
Hauptverfasser:	OHTA, Hiroshi, WAKITANI, Shigeyuki, TENSHO, Keiji, HORIUCHI, Hiroshi, WAKABAYASHI, Shinji, SAITO, Naoto, NAKAMURA, Yukio, NOZAKI, Kazutoshi, IMAI, Yunki, TAKAOKA, Kunio
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - biosynthesis Animals Biological and medical sciences Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins - isolation & purification Bone Morphogenetic Proteins - pharmacology Calcification, Physiologic Cell Line Diseases of the osteoarticular system Electrophoresis, Polyacrylamide Gel Enzyme Induction - drug effects Hot Temperature Humans Medical research Medical sciences Mice Proteins Recombinant Proteins - pharmacology Transforming Growth Factor beta - isolation & purification Transforming Growth Factor beta - pharmacology
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