Practical Enzymatic Synthesis of UDP Sugars and NDP Glucoses

Practical Enzymatic Synthesis of UDP Sugars and NDP Glucoses

Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar‐1‐phosphates (Su1Ps) by recombinant UDP‐sugar pyrophosphorylase (UP) from Thermus caldophilus GK24, t...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2005-11, Vol.6 (11), p.1963-1966
Hauptverfasser: Bae, Jungdon, Kim, Kwang-Ho, Kim, Dooil, Choi, Yongseok, Kim, Joong Su, Koh, Sukhoon, Hong, Suk-In, Lee, Dae-Sil
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Bacteria - enzymology
enzyme catalysis
Glucose - analogs & derivatives
Glucose - chemistry
glycosyltransferases
Molecular Sequence Data
Nucleoside Diphosphate Sugars - chemical synthesis
nucleotide sugars
Nucleotidyltransferases - chemistry
Sequence Alignment
Thermus caldophilus
Uridine Diphosphate Sugars - chemical synthesis
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Zusammenfassung:Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar‐1‐phosphates (Su1Ps) by recombinant UDP‐sugar pyrophosphorylase (UP) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose‐1‐phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200500183