Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses
To enhance fungal disease resistance, wheat plants (cv. Bobwhite) were engineered to constitutively express the potent antimicrobial protein Ace-AMP1 from Allium cepa, driven by a maize ubiquitin promoter along with its first intron. The bar gene was used for selection of putative transformants on m...
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| Veröffentlicht in: | Transgenic research 2006-08, Vol.15 (4), p.435-446, Article 435 |
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| creator | ROY-BARMAN, Subhankar SAUTTER, Christof CHATTOO, Bharat B |
| description | To enhance fungal disease resistance, wheat plants (cv. Bobwhite) were engineered to constitutively express the potent antimicrobial protein Ace-AMP1 from Allium cepa, driven by a maize ubiquitin promoter along with its first intron. The bar gene was used for selection of putative transformants on medium containing phosphinothricin (PPT). Transgene inheritance, integration and stability of expression were confirmed over two generations by PCR, Southern, northern and western blot analyses, respectively. The levels of Ace-AMP1 in different transgenic lines correlated with the transcript levels of the transgene. Up to 50% increase in resistance to Blumeria graminis f. sp. tritici was detected in detached leaf assays. In ears of transgenic wheat inoculated with Neovossia indica, Ace-AMP1 intensified expression of defense-related genes. Elevated levels of salicylic acid and of transcripts of phenylalanine ammonia lyase (PAL), glucanase (PR2) and chitinase (PR3) in the transgenic plants indicated manifestation of systemic acquired resistance (SAR). |
| doi_str_mv | 10.1007/s11248-006-0016-1 |
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Bobwhite) were engineered to constitutively express the potent antimicrobial protein Ace-AMP1 from Allium cepa, driven by a maize ubiquitin promoter along with its first intron. The bar gene was used for selection of putative transformants on medium containing phosphinothricin (PPT). Transgene inheritance, integration and stability of expression were confirmed over two generations by PCR, Southern, northern and western blot analyses, respectively. The levels of Ace-AMP1 in different transgenic lines correlated with the transcript levels of the transgene. Up to 50% increase in resistance to Blumeria graminis f. sp. tritici was detected in detached leaf assays. In ears of transgenic wheat inoculated with Neovossia indica, Ace-AMP1 intensified expression of defense-related genes. Elevated levels of salicylic acid and of transcripts of phenylalanine ammonia lyase (PAL), glucanase (PR2) and chitinase (PR3) in the transgenic plants indicated manifestation of systemic acquired resistance (SAR).</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-006-0016-1</identifier><identifier>PMID: 16906444</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Allium cepa ; Ammonia ; Antifungal activity ; Antifungal Agents - pharmacology ; Bar gene ; Biological and medical sciences ; Biotechnology ; Blotting, Northern ; Blumeria graminis ; Chitinase ; Crop diseases ; Disease resistance ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Genetic Techniques ; Genetic Vectors ; Heredity ; Immunity, Innate ; Methods. Procedures. Technologies ; Models, Genetic ; Phenolsulfonphthalein - analogs & derivatives ; Phenolsulfonphthalein - pharmacology ; Phenylalanine ; Phenylalanine Ammonia-Lyase - genetics ; Phosphinothricin ; Plant diseases ; Plant Proteins - genetics ; Plants, Genetically Modified ; Plasmids - metabolism ; Polymerase Chain Reaction ; Salicylic acid ; Salicylic Acid - metabolism ; Transgenes ; Transgenic animals and transgenic plants ; Transgenic plants ; Triticum - genetics ; Triticum aestivum ; Ubiquitin ; Zea mays</subject><ispartof>Transgenic research, 2006-08, Vol.15 (4), p.435-446, Article 435</ispartof><rights>2006 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2006.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-1f17fb5b8b36ba400c12d1f305fbb8e5eb9eeeb64b95fc248cf15101383e13163</citedby><cites>FETCH-LOGICAL-c387t-1f17fb5b8b36ba400c12d1f305fbb8e5eb9eeeb64b95fc248cf15101383e13163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18058989$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16906444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ROY-BARMAN, Subhankar</creatorcontrib><creatorcontrib>SAUTTER, Christof</creatorcontrib><creatorcontrib>CHATTOO, Bharat B</creatorcontrib><title>Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><description>To enhance fungal disease resistance, wheat plants (cv. Bobwhite) were engineered to constitutively express the potent antimicrobial protein Ace-AMP1 from Allium cepa, driven by a maize ubiquitin promoter along with its first intron. The bar gene was used for selection of putative transformants on medium containing phosphinothricin (PPT). Transgene inheritance, integration and stability of expression were confirmed over two generations by PCR, Southern, northern and western blot analyses, respectively. The levels of Ace-AMP1 in different transgenic lines correlated with the transcript levels of the transgene. Up to 50% increase in resistance to Blumeria graminis f. sp. tritici was detected in detached leaf assays. In ears of transgenic wheat inoculated with Neovossia indica, Ace-AMP1 intensified expression of defense-related genes. Elevated levels of salicylic acid and of transcripts of phenylalanine ammonia lyase (PAL), glucanase (PR2) and chitinase (PR3) in the transgenic plants indicated manifestation of systemic acquired resistance (SAR).</description><subject>Allium cepa</subject><subject>Ammonia</subject><subject>Antifungal activity</subject><subject>Antifungal Agents - pharmacology</subject><subject>Bar gene</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Northern</subject><subject>Blumeria graminis</subject><subject>Chitinase</subject><subject>Crop diseases</subject><subject>Disease resistance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Techniques</subject><subject>Genetic Vectors</subject><subject>Heredity</subject><subject>Immunity, Innate</subject><subject>Methods. Procedures. Technologies</subject><subject>Models, Genetic</subject><subject>Phenolsulfonphthalein - analogs & derivatives</subject><subject>Phenolsulfonphthalein - pharmacology</subject><subject>Phenylalanine</subject><subject>Phenylalanine Ammonia-Lyase - genetics</subject><subject>Phosphinothricin</subject><subject>Plant diseases</subject><subject>Plant Proteins - genetics</subject><subject>Plants, Genetically Modified</subject><subject>Plasmids - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Salicylic acid</subject><subject>Salicylic Acid - metabolism</subject><subject>Transgenes</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>Triticum - genetics</subject><subject>Triticum aestivum</subject><subject>Ubiquitin</subject><subject>Zea mays</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUFvFCEUx4nR2LX6AbwYYmJvU3nLDAPHTVOrSZv2oGcCzKNLM8uMwGj77WXdTZp48UB4gR9_8t6PkPfAzoGx_nMGWLeyYUzUBaKBF2QFXc8bxYV8SVZMiXUjJagT8ibnh8owJvlrcgJCMdG27Yo8Xj7OCXMOU6STp2WLdAxzGGhJJmaPic5pKhgi3ThsNjd3QGv99_IeY3D09xZNoRi3JjrM1MQS_BLvzUiNK-FXKE_1bKADeowZaf1rnmqR35JX3owZ3x33U_Ljy-X3i6_N9e3Vt4vNdeO47EsDHnpvOystF9a0jDlYD-A567y1Eju0ChGtaK3qvKvTcB46YMAlR-Ag-Ck5O-TWPn4umIvehexwHE3EaclayL7tBVP_BUHJltVhVvDjP-DDtKRYm9B9t2Y1qpcVggPk0pRzQq_nFHYmPWlgei9PH-TpKk_v5el98Idj8GJ3ODy_ONqqwKcjYLIzo68WXMjPnGSdVFLxPx6Joqw</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>ROY-BARMAN, Subhankar</creator><creator>SAUTTER, Christof</creator><creator>CHATTOO, Bharat B</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QO</scope><scope>7T7</scope><scope>C1K</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses</title><author>ROY-BARMAN, Subhankar ; SAUTTER, Christof ; CHATTOO, Bharat B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-1f17fb5b8b36ba400c12d1f305fbb8e5eb9eeeb64b95fc248cf15101383e13163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Allium cepa</topic><topic>Ammonia</topic><topic>Antifungal activity</topic><topic>Antifungal Agents - pharmacology</topic><topic>Bar gene</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Northern</topic><topic>Blumeria graminis</topic><topic>Chitinase</topic><topic>Crop diseases</topic><topic>Disease resistance</topic><topic>Fundamental and applied biological sciences. 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Technologies</topic><topic>Models, Genetic</topic><topic>Phenolsulfonphthalein - analogs & derivatives</topic><topic>Phenolsulfonphthalein - pharmacology</topic><topic>Phenylalanine</topic><topic>Phenylalanine Ammonia-Lyase - genetics</topic><topic>Phosphinothricin</topic><topic>Plant diseases</topic><topic>Plant Proteins - genetics</topic><topic>Plants, Genetically Modified</topic><topic>Plasmids - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Salicylic acid</topic><topic>Salicylic Acid - metabolism</topic><topic>Transgenes</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><topic>Triticum - genetics</topic><topic>Triticum aestivum</topic><topic>Ubiquitin</topic><topic>Zea mays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROY-BARMAN, Subhankar</creatorcontrib><creatorcontrib>SAUTTER, Christof</creatorcontrib><creatorcontrib>CHATTOO, Bharat B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROY-BARMAN, Subhankar</au><au>SAUTTER, Christof</au><au>CHATTOO, Bharat B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses</atitle><jtitle>Transgenic research</jtitle><addtitle>Transgenic Res</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>15</volume><issue>4</issue><spage>435</spage><epage>446</epage><pages>435-446</pages><artnum>435</artnum><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>To enhance fungal disease resistance, wheat plants (cv. Bobwhite) were engineered to constitutively express the potent antimicrobial protein Ace-AMP1 from Allium cepa, driven by a maize ubiquitin promoter along with its first intron. The bar gene was used for selection of putative transformants on medium containing phosphinothricin (PPT). Transgene inheritance, integration and stability of expression were confirmed over two generations by PCR, Southern, northern and western blot analyses, respectively. The levels of Ace-AMP1 in different transgenic lines correlated with the transcript levels of the transgene. Up to 50% increase in resistance to Blumeria graminis f. sp. tritici was detected in detached leaf assays. In ears of transgenic wheat inoculated with Neovossia indica, Ace-AMP1 intensified expression of defense-related genes. Elevated levels of salicylic acid and of transcripts of phenylalanine ammonia lyase (PAL), glucanase (PR2) and chitinase (PR3) in the transgenic plants indicated manifestation of systemic acquired resistance (SAR).</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>16906444</pmid><doi>10.1007/s11248-006-0016-1</doi><tpages>12</tpages></addata></record> |
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| subjects | Allium cepa Ammonia Antifungal activity Antifungal Agents - pharmacology Bar gene Biological and medical sciences Biotechnology Blotting, Northern Blumeria graminis Chitinase Crop diseases Disease resistance Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Techniques Genetic Vectors Heredity Immunity, Innate Methods. Procedures. Technologies Models, Genetic Phenolsulfonphthalein - analogs & derivatives Phenolsulfonphthalein - pharmacology Phenylalanine Phenylalanine Ammonia-Lyase - genetics Phosphinothricin Plant diseases Plant Proteins - genetics Plants, Genetically Modified Plasmids - metabolism Polymerase Chain Reaction Salicylic acid Salicylic Acid - metabolism Transgenes Transgenic animals and transgenic plants Transgenic plants Triticum - genetics Triticum aestivum Ubiquitin Zea mays |
| title | Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses |
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