Cloning and biochemical properties of CDPK gene OsCDPK14 from rice
A rice CDPK gene, OsCDPK14 (AY144497), was cloned from developing caryopses of rice ( Oryza sativa cv. Zhonghua 15). Its cDNA sequence (1922 bp) contains an ORF encoding a 514 amino acids protein (56.7 kD, pl 5.18). OsCDPK14 shows the typical structural features of the CDPK family, including a conse...
Gespeichert in:
| Veröffentlicht in: | Journal of plant physiology 2005-10, Vol.162 (10), p.1149-1159 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1159 |
|---|---|
| container_issue | 10 |
| container_start_page | 1149 |
| container_title | Journal of plant physiology |
| container_volume | 162 |
| creator | Zhang, Tiegang Wang, Qi Chen, Xing Tian, Chunhua Wang, Xinshen Xing, Tim Li, Yi Wang, Yingdian |
| description | A rice CDPK gene,
OsCDPK14 (AY144497), was cloned from developing caryopses of rice (
Oryza sativa cv. Zhonghua 15). Its cDNA sequence (1922
bp) contains an ORF encoding a 514 amino acids protein (56.7
kD, pl 5.18). OsCDPK14 shows the typical structural features of the CDPK family, including a conserved catalytic Ser/Thr kinase domain, an autoinhibitory domain and a CaM-like domain with four putative Ca
2+-binding EF hands. Subcellular targeting indicated that OsCDPK14 was located in the cytoplasm, probably due to the absence of myristoylation and palmitoylation motifs.
OsCDPK14 was expressed in
Escherichia coli and purified from bacterial extracts. The recombinant protein was shown to be a functional protein kinase using Syntide-2, a synthetic peptide. Kinase activity was shown to be Ca
2+-dependent, and this activation was strongly enhanced by Mn
2+ and inhibited by W7 in vitro. These results provide significant insights into the regulation and biochemical properties of OsCDPK14, suggesting OsCDPK14 may be a signal factor of cytoplasm in rice plant. |
| doi_str_mv | 10.1016/j.jplph.2004.12.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68745847</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0176161705000623</els_id><sourcerecordid>68745847</sourcerecordid><originalsourceid>FETCH-LOGICAL-c442t-d218002749c17677d0fb34a3a425ae4aef5cdf61dc6a8e5c7d5180c9c1643553</originalsourceid><addsrcrecordid>eNqFkE2P0zAQhi0EYsvCL0CCXOCW4PFneuAA5VOstEgsZ8u1x11XSZy1WyT-PQ6ttDc42R49M_P6IeQ50A4oqDf7bj8P823HKBUdsI4CfUBWoKBvgbP-IVlR0KqtBX1BnpSyp_Ute_6YXIBiUoLmK_J-M6QpTrvGTr7ZxuRucYzODs2c04z5ELE0KTSbD9-_NTucsLkuyx1EE3IamxwdPiWPgh0KPjufl-Tm08ebzZf26vrz1827q9YJwQ6tZ9BTyrRYuxpLa0_DlgvLrWDSorAYpPNBgXfK9iid9rI2uEorwaXkl-T1aWxNdnfEcjBjLA6HwU6YjsWoXgvZC_1fENZaw1qqCvIT6HIqJWMwc46jzb8NULMoNnvzV7FZFBtgpiquXS_O44_bEf19z9lpBV6dAVuqyZDt5GK553SFhFxyvjxxwSZjd7kyP38wCrxukXXh8uW3JwKr1l8Rsyku4uTQx4zuYHyK_4z6B_xxoTg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19771956</pqid></control><display><type>article</type><title>Cloning and biochemical properties of CDPK gene OsCDPK14 from rice</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Zhang, Tiegang ; Wang, Qi ; Chen, Xing ; Tian, Chunhua ; Wang, Xinshen ; Xing, Tim ; Li, Yi ; Wang, Yingdian</creator><creatorcontrib>Zhang, Tiegang ; Wang, Qi ; Chen, Xing ; Tian, Chunhua ; Wang, Xinshen ; Xing, Tim ; Li, Yi ; Wang, Yingdian</creatorcontrib><description>A rice CDPK gene,
OsCDPK14 (AY144497), was cloned from developing caryopses of rice (
Oryza sativa cv. Zhonghua 15). Its cDNA sequence (1922
bp) contains an ORF encoding a 514 amino acids protein (56.7
kD, pl 5.18). OsCDPK14 shows the typical structural features of the CDPK family, including a conserved catalytic Ser/Thr kinase domain, an autoinhibitory domain and a CaM-like domain with four putative Ca
2+-binding EF hands. Subcellular targeting indicated that OsCDPK14 was located in the cytoplasm, probably due to the absence of myristoylation and palmitoylation motifs.
OsCDPK14 was expressed in
Escherichia coli and purified from bacterial extracts. The recombinant protein was shown to be a functional protein kinase using Syntide-2, a synthetic peptide. Kinase activity was shown to be Ca
2+-dependent, and this activation was strongly enhanced by Mn
2+ and inhibited by W7 in vitro. These results provide significant insights into the regulation and biochemical properties of OsCDPK14, suggesting OsCDPK14 may be a signal factor of cytoplasm in rice plant.</description><identifier>ISSN: 0176-1617</identifier><identifier>EISSN: 1618-1328</identifier><identifier>DOI: 10.1016/j.jplph.2004.12.010</identifier><identifier>PMID: 16255173</identifier><identifier>CODEN: JPPHEY</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Agronomy. Soil science and plant productions ; Amino Acid Sequence ; amino acid sequences ; Base Sequence ; Biological and medical sciences ; calcium-binding proteins ; Calcium-dependent protein kinase (CDPK) ; cDNA cloning ; Classical and quantitative genetics. Population genetics. Molecular genetics ; Cloning, Molecular ; complementary DNA ; cyclin-dependent protein kinases ; DNA Primers ; DNA, Plant ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Generalities. Genetics. Plant material ; Genetics and breeding of economic plants ; grain crops ; Kinase activities ; Molecular genetics ; Molecular Sequence Data ; Nicotiana tabacum ; nucleotide sequences ; Oryza - genetics ; Oryza sativa ; OsCDPK14 ; plant biochemistry ; plant genetics ; protein kinases ; Protein Kinases - chemistry ; Protein Kinases - genetics ; Protein Kinases - isolation & purification ; protoplasts ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - isolation & purification ; rice ; Rice ( Oryza sativa) ; sequence alignment ; Sequence Homology, Amino Acid ; serine-threonine protein kinases ; Subcellular Fractions - enzymology ; Subcellular localization ; tissue distribution ; tobacco ; transgenic plants</subject><ispartof>Journal of plant physiology, 2005-10, Vol.162 (10), p.1149-1159</ispartof><rights>2005 Elsevier GmbH</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-d218002749c17677d0fb34a3a425ae4aef5cdf61dc6a8e5c7d5180c9c1643553</citedby><cites>FETCH-LOGICAL-c442t-d218002749c17677d0fb34a3a425ae4aef5cdf61dc6a8e5c7d5180c9c1643553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jplph.2004.12.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17173457$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16255173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Tiegang</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Chen, Xing</creatorcontrib><creatorcontrib>Tian, Chunhua</creatorcontrib><creatorcontrib>Wang, Xinshen</creatorcontrib><creatorcontrib>Xing, Tim</creatorcontrib><creatorcontrib>Li, Yi</creatorcontrib><creatorcontrib>Wang, Yingdian</creatorcontrib><title>Cloning and biochemical properties of CDPK gene OsCDPK14 from rice</title><title>Journal of plant physiology</title><addtitle>J Plant Physiol</addtitle><description>A rice CDPK gene,
OsCDPK14 (AY144497), was cloned from developing caryopses of rice (
Oryza sativa cv. Zhonghua 15). Its cDNA sequence (1922
bp) contains an ORF encoding a 514 amino acids protein (56.7
kD, pl 5.18). OsCDPK14 shows the typical structural features of the CDPK family, including a conserved catalytic Ser/Thr kinase domain, an autoinhibitory domain and a CaM-like domain with four putative Ca
2+-binding EF hands. Subcellular targeting indicated that OsCDPK14 was located in the cytoplasm, probably due to the absence of myristoylation and palmitoylation motifs.
OsCDPK14 was expressed in
Escherichia coli and purified from bacterial extracts. The recombinant protein was shown to be a functional protein kinase using Syntide-2, a synthetic peptide. Kinase activity was shown to be Ca
2+-dependent, and this activation was strongly enhanced by Mn
2+ and inhibited by W7 in vitro. These results provide significant insights into the regulation and biochemical properties of OsCDPK14, suggesting OsCDPK14 may be a signal factor of cytoplasm in rice plant.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>calcium-binding proteins</subject><subject>Calcium-dependent protein kinase (CDPK)</subject><subject>cDNA cloning</subject><subject>Classical and quantitative genetics. Population genetics. Molecular genetics</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>cyclin-dependent protein kinases</subject><subject>DNA Primers</subject><subject>DNA, Plant</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genetics and breeding of economic plants</subject><subject>grain crops</subject><subject>Kinase activities</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana tabacum</subject><subject>nucleotide sequences</subject><subject>Oryza - genetics</subject><subject>Oryza sativa</subject><subject>OsCDPK14</subject><subject>plant biochemistry</subject><subject>plant genetics</subject><subject>protein kinases</subject><subject>Protein Kinases - chemistry</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - isolation & purification</subject><subject>protoplasts</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>rice</subject><subject>Rice ( Oryza sativa)</subject><subject>sequence alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>serine-threonine protein kinases</subject><subject>Subcellular Fractions - enzymology</subject><subject>Subcellular localization</subject><subject>tissue distribution</subject><subject>tobacco</subject><subject>transgenic plants</subject><issn>0176-1617</issn><issn>1618-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2P0zAQhi0EYsvCL0CCXOCW4PFneuAA5VOstEgsZ8u1x11XSZy1WyT-PQ6ttDc42R49M_P6IeQ50A4oqDf7bj8P823HKBUdsI4CfUBWoKBvgbP-IVlR0KqtBX1BnpSyp_Ute_6YXIBiUoLmK_J-M6QpTrvGTr7ZxuRucYzODs2c04z5ELE0KTSbD9-_NTucsLkuyx1EE3IamxwdPiWPgh0KPjufl-Tm08ebzZf26vrz1827q9YJwQ6tZ9BTyrRYuxpLa0_DlgvLrWDSorAYpPNBgXfK9iid9rI2uEorwaXkl-T1aWxNdnfEcjBjLA6HwU6YjsWoXgvZC_1fENZaw1qqCvIT6HIqJWMwc46jzb8NULMoNnvzV7FZFBtgpiquXS_O44_bEf19z9lpBV6dAVuqyZDt5GK553SFhFxyvjxxwSZjd7kyP38wCrxukXXh8uW3JwKr1l8Rsyku4uTQx4zuYHyK_4z6B_xxoTg</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Zhang, Tiegang</creator><creator>Wang, Qi</creator><creator>Chen, Xing</creator><creator>Tian, Chunhua</creator><creator>Wang, Xinshen</creator><creator>Xing, Tim</creator><creator>Li, Yi</creator><creator>Wang, Yingdian</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20051001</creationdate><title>Cloning and biochemical properties of CDPK gene OsCDPK14 from rice</title><author>Zhang, Tiegang ; Wang, Qi ; Chen, Xing ; Tian, Chunhua ; Wang, Xinshen ; Xing, Tim ; Li, Yi ; Wang, Yingdian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-d218002749c17677d0fb34a3a425ae4aef5cdf61dc6a8e5c7d5180c9c1643553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>calcium-binding proteins</topic><topic>Calcium-dependent protein kinase (CDPK)</topic><topic>cDNA cloning</topic><topic>Classical and quantitative genetics. Population genetics. Molecular genetics</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>cyclin-dependent protein kinases</topic><topic>DNA Primers</topic><topic>DNA, Plant</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genetics and breeding of economic plants</topic><topic>grain crops</topic><topic>Kinase activities</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana tabacum</topic><topic>nucleotide sequences</topic><topic>Oryza - genetics</topic><topic>Oryza sativa</topic><topic>OsCDPK14</topic><topic>plant biochemistry</topic><topic>plant genetics</topic><topic>protein kinases</topic><topic>Protein Kinases - chemistry</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - isolation & purification</topic><topic>protoplasts</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>rice</topic><topic>Rice ( Oryza sativa)</topic><topic>sequence alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>serine-threonine protein kinases</topic><topic>Subcellular Fractions - enzymology</topic><topic>Subcellular localization</topic><topic>tissue distribution</topic><topic>tobacco</topic><topic>transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Tiegang</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Chen, Xing</creatorcontrib><creatorcontrib>Tian, Chunhua</creatorcontrib><creatorcontrib>Wang, Xinshen</creatorcontrib><creatorcontrib>Xing, Tim</creatorcontrib><creatorcontrib>Li, Yi</creatorcontrib><creatorcontrib>Wang, Yingdian</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Tiegang</au><au>Wang, Qi</au><au>Chen, Xing</au><au>Tian, Chunhua</au><au>Wang, Xinshen</au><au>Xing, Tim</au><au>Li, Yi</au><au>Wang, Yingdian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and biochemical properties of CDPK gene OsCDPK14 from rice</atitle><jtitle>Journal of plant physiology</jtitle><addtitle>J Plant Physiol</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>162</volume><issue>10</issue><spage>1149</spage><epage>1159</epage><pages>1149-1159</pages><issn>0176-1617</issn><eissn>1618-1328</eissn><coden>JPPHEY</coden><abstract>A rice CDPK gene,
OsCDPK14 (AY144497), was cloned from developing caryopses of rice (
Oryza sativa cv. Zhonghua 15). Its cDNA sequence (1922
bp) contains an ORF encoding a 514 amino acids protein (56.7
kD, pl 5.18). OsCDPK14 shows the typical structural features of the CDPK family, including a conserved catalytic Ser/Thr kinase domain, an autoinhibitory domain and a CaM-like domain with four putative Ca
2+-binding EF hands. Subcellular targeting indicated that OsCDPK14 was located in the cytoplasm, probably due to the absence of myristoylation and palmitoylation motifs.
OsCDPK14 was expressed in
Escherichia coli and purified from bacterial extracts. The recombinant protein was shown to be a functional protein kinase using Syntide-2, a synthetic peptide. Kinase activity was shown to be Ca
2+-dependent, and this activation was strongly enhanced by Mn
2+ and inhibited by W7 in vitro. These results provide significant insights into the regulation and biochemical properties of OsCDPK14, suggesting OsCDPK14 may be a signal factor of cytoplasm in rice plant.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>16255173</pmid><doi>10.1016/j.jplph.2004.12.010</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0176-1617 |
| ispartof | Journal of plant physiology, 2005-10, Vol.162 (10), p.1149-1159 |
| issn | 0176-1617 1618-1328 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68745847 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agronomy. Soil science and plant productions Amino Acid Sequence amino acid sequences Base Sequence Biological and medical sciences calcium-binding proteins Calcium-dependent protein kinase (CDPK) cDNA cloning Classical and quantitative genetics. Population genetics. Molecular genetics Cloning, Molecular complementary DNA cyclin-dependent protein kinases DNA Primers DNA, Plant Escherichia coli Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetics and breeding of economic plants grain crops Kinase activities Molecular genetics Molecular Sequence Data Nicotiana tabacum nucleotide sequences Oryza - genetics Oryza sativa OsCDPK14 plant biochemistry plant genetics protein kinases Protein Kinases - chemistry Protein Kinases - genetics Protein Kinases - isolation & purification protoplasts Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification rice Rice ( Oryza sativa) sequence alignment Sequence Homology, Amino Acid serine-threonine protein kinases Subcellular Fractions - enzymology Subcellular localization tissue distribution tobacco transgenic plants |
| title | Cloning and biochemical properties of CDPK gene OsCDPK14 from rice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T22%3A39%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20and%20biochemical%20properties%20of%20CDPK%20gene%20OsCDPK14%20from%20rice&rft.jtitle=Journal%20of%20plant%20physiology&rft.au=Zhang,%20Tiegang&rft.date=2005-10-01&rft.volume=162&rft.issue=10&rft.spage=1149&rft.epage=1159&rft.pages=1149-1159&rft.issn=0176-1617&rft.eissn=1618-1328&rft.coden=JPPHEY&rft_id=info:doi/10.1016/j.jplph.2004.12.010&rft_dat=%3Cproquest_cross%3E68745847%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19771956&rft_id=info:pmid/16255173&rft_els_id=S0176161705000623&rfr_iscdi=true |