c-JUN N-terminal kinase-1 (JNK1) but not JNK2 or JNK3 is involved in UV signal transduction in human epidermis
c-Jun N-terminal kinase (JNK) plays a critical role in UV-induced apoptotic cell death. Although three isoforms are known in mammals, physiological roles of each isoform are still obscure. Furthermore, our recent findings show that serpin squamous cell carcinoma antigen (SCCA1) binds to JNK. To dete...
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| Veröffentlicht in: | Journal of dermatological science 2006-09, Vol.43 (3), p.171-179 |
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| creator | Katagiri, Chika Negishi, Kei Hibino, Toshihiko |
| description | c-Jun N-terminal kinase (JNK) plays a critical role in UV-induced apoptotic cell death. Although three isoforms are known in mammals, physiological roles of each isoform are still obscure. Furthermore, our recent findings show that serpin squamous cell carcinoma antigen (SCCA1) binds to JNK.
To determine which isoform is responsible for the UV signal transduction in human epidermis and whether SCCA1 is capable to regulate kinase activity of a specific isoform.
Immunohistochemical localization of each JNK isoform was investigated after UV irradiation
in vivo and
in vitro. Effect of recombinant SCCA1 on JNK kinase activity was also analyzed.
Immunostaining for JNK1, 2 and 3 demonstrated marked elevation of JNK1 in spinous to granular cells of UV-irradiated skin, whereas they were expressed weakly in upper epidermis of the sun-protected, buttock skin. In cultured keratinocytes, only JNK1 is translocated into nucleus after UV irradiation. JNK2, which localized in the cytoplasm, or JNK3, which was confined in nucleus, remained in the same compartment after UV irradiation. We confirmed that only JNK1 mRNA was up-regulated after UV irradiation in cultured keratinocytes. In addition, recombinant SCCA1 suppressed kinase activity of JNK1 but did not affect JNK2 or JNK3 kinase activity.
JNK1 is associated with UV signal transduction in human epidermis and SCCA1 is a suppressor of this process. |
| doi_str_mv | 10.1016/j.jdermsci.2006.05.008 |
| format | Article |
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To determine which isoform is responsible for the UV signal transduction in human epidermis and whether SCCA1 is capable to regulate kinase activity of a specific isoform.
Immunohistochemical localization of each JNK isoform was investigated after UV irradiation
in vivo and
in vitro. Effect of recombinant SCCA1 on JNK kinase activity was also analyzed.
Immunostaining for JNK1, 2 and 3 demonstrated marked elevation of JNK1 in spinous to granular cells of UV-irradiated skin, whereas they were expressed weakly in upper epidermis of the sun-protected, buttock skin. In cultured keratinocytes, only JNK1 is translocated into nucleus after UV irradiation. JNK2, which localized in the cytoplasm, or JNK3, which was confined in nucleus, remained in the same compartment after UV irradiation. We confirmed that only JNK1 mRNA was up-regulated after UV irradiation in cultured keratinocytes. In addition, recombinant SCCA1 suppressed kinase activity of JNK1 but did not affect JNK2 or JNK3 kinase activity.
JNK1 is associated with UV signal transduction in human epidermis and SCCA1 is a suppressor of this process.</description><identifier>ISSN: 0923-1811</identifier><identifier>EISSN: 1873-569X</identifier><identifier>DOI: 10.1016/j.jdermsci.2006.05.008</identifier><identifier>PMID: 16824735</identifier><language>eng</language><publisher>Netherlands: Elsevier Ireland Ltd</publisher><subject>Active Transport, Cell Nucleus ; Adult ; Antigens, Neoplasm - genetics ; Antigens, Neoplasm - pharmacology ; Antigens, Neoplasm - physiology ; Apoptosis ; c-Jun N-terminal kinase ; Cell Nucleus - enzymology ; Cytoplasm - enzymology ; Epidermis - drug effects ; Epidermis - enzymology ; Epidermis - radiation effects ; Female ; Humans ; Male ; Middle Aged ; Mitogen-Activated Protein Kinase 10 - analysis ; Mitogen-Activated Protein Kinase 10 - metabolism ; Mitogen-Activated Protein Kinase 8 - analysis ; Mitogen-Activated Protein Kinase 8 - antagonists & inhibitors ; Mitogen-Activated Protein Kinase 8 - metabolism ; Mitogen-Activated Protein Kinase 9 - analysis ; Mitogen-Activated Protein Kinase 9 - metabolism ; RNA, Messenger - agonists ; RNA, Messenger - analysis ; Serpins - genetics ; Serpins - pharmacology ; Serpins - physiology ; Signal Transduction ; Squamous cell carcinoma antigen ; Ultraviolet irradiation ; Ultraviolet Rays ; Up-Regulation</subject><ispartof>Journal of dermatological science, 2006-09, Vol.43 (3), p.171-179</ispartof><rights>2006 Japanese Society for Investigative Dermatology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-9ea97c035c4e2a1fe54608da39ddfa4c10ecae892d3281147140265d2e6f2573</citedby><cites>FETCH-LOGICAL-c419t-9ea97c035c4e2a1fe54608da39ddfa4c10ecae892d3281147140265d2e6f2573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jdermsci.2006.05.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16824735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katagiri, Chika</creatorcontrib><creatorcontrib>Negishi, Kei</creatorcontrib><creatorcontrib>Hibino, Toshihiko</creatorcontrib><title>c-JUN N-terminal kinase-1 (JNK1) but not JNK2 or JNK3 is involved in UV signal transduction in human epidermis</title><title>Journal of dermatological science</title><addtitle>J Dermatol Sci</addtitle><description>c-Jun N-terminal kinase (JNK) plays a critical role in UV-induced apoptotic cell death. Although three isoforms are known in mammals, physiological roles of each isoform are still obscure. Furthermore, our recent findings show that serpin squamous cell carcinoma antigen (SCCA1) binds to JNK.
To determine which isoform is responsible for the UV signal transduction in human epidermis and whether SCCA1 is capable to regulate kinase activity of a specific isoform.
Immunohistochemical localization of each JNK isoform was investigated after UV irradiation
in vivo and
in vitro. Effect of recombinant SCCA1 on JNK kinase activity was also analyzed.
Immunostaining for JNK1, 2 and 3 demonstrated marked elevation of JNK1 in spinous to granular cells of UV-irradiated skin, whereas they were expressed weakly in upper epidermis of the sun-protected, buttock skin. In cultured keratinocytes, only JNK1 is translocated into nucleus after UV irradiation. JNK2, which localized in the cytoplasm, or JNK3, which was confined in nucleus, remained in the same compartment after UV irradiation. We confirmed that only JNK1 mRNA was up-regulated after UV irradiation in cultured keratinocytes. In addition, recombinant SCCA1 suppressed kinase activity of JNK1 but did not affect JNK2 or JNK3 kinase activity.
JNK1 is associated with UV signal transduction in human epidermis and SCCA1 is a suppressor of this process.</description><subject>Active Transport, Cell Nucleus</subject><subject>Adult</subject><subject>Antigens, Neoplasm - genetics</subject><subject>Antigens, Neoplasm - pharmacology</subject><subject>Antigens, Neoplasm - physiology</subject><subject>Apoptosis</subject><subject>c-Jun N-terminal kinase</subject><subject>Cell Nucleus - enzymology</subject><subject>Cytoplasm - enzymology</subject><subject>Epidermis - drug effects</subject><subject>Epidermis - enzymology</subject><subject>Epidermis - radiation effects</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mitogen-Activated Protein Kinase 10 - analysis</subject><subject>Mitogen-Activated Protein Kinase 10 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 8 - analysis</subject><subject>Mitogen-Activated Protein Kinase 8 - antagonists & inhibitors</subject><subject>Mitogen-Activated Protein Kinase 8 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 9 - analysis</subject><subject>Mitogen-Activated Protein Kinase 9 - metabolism</subject><subject>RNA, Messenger - agonists</subject><subject>RNA, Messenger - analysis</subject><subject>Serpins - genetics</subject><subject>Serpins - pharmacology</subject><subject>Serpins - physiology</subject><subject>Signal Transduction</subject><subject>Squamous cell carcinoma antigen</subject><subject>Ultraviolet irradiation</subject><subject>Ultraviolet Rays</subject><subject>Up-Regulation</subject><issn>0923-1811</issn><issn>1873-569X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUMtO3DAUtSoQTIFfQF4huki4dmwn2VGhlpaiYQNVd5axb1pPM87UTkbq3-N0BnXJ5j7k8_A9hJwzKBkwdbUqVw7jOllfcgBVgiwBmndkwZq6KqRqfxyQBbS8KljD2DF5n9IKACQX7RE5Zqrhoq7kggRb3D0t6bIYs5oPpqe_c01YMHp5t_zGPtDnaaRhGGneOB3i3CvqE_VhO_RbdHmgT99p8j9n9hhNSG6yox_C_PJrWptAcePn3_p0Sg470yc82_cT8vj50-PNl-L-4fbrzcf7wgrWjkWLpq0tVNIK5IZ1KIWCxpmqda4zwjJAa7Bpuat4Pk_UTABX0nFUHZd1dUIudrKbOPyZMI06e1vsexNwmJJWTS04gyYD1Q5o45BSxE5vol-b-Fcz0HPQeqVfg9Zz0Bqkhn_E873D9LxG95-2TzYDrncAzGduPUadJTBYdD6iHbUb_FseL6MLkKA</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Katagiri, Chika</creator><creator>Negishi, Kei</creator><creator>Hibino, Toshihiko</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>c-JUN N-terminal kinase-1 (JNK1) but not JNK2 or JNK3 is involved in UV signal transduction in human epidermis</title><author>Katagiri, Chika ; Negishi, Kei ; Hibino, Toshihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-9ea97c035c4e2a1fe54608da39ddfa4c10ecae892d3281147140265d2e6f2573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Active Transport, Cell Nucleus</topic><topic>Adult</topic><topic>Antigens, Neoplasm - genetics</topic><topic>Antigens, Neoplasm - pharmacology</topic><topic>Antigens, Neoplasm - physiology</topic><topic>Apoptosis</topic><topic>c-Jun N-terminal kinase</topic><topic>Cell Nucleus - enzymology</topic><topic>Cytoplasm - enzymology</topic><topic>Epidermis - drug effects</topic><topic>Epidermis - enzymology</topic><topic>Epidermis - radiation effects</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mitogen-Activated Protein Kinase 10 - analysis</topic><topic>Mitogen-Activated Protein Kinase 10 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 8 - analysis</topic><topic>Mitogen-Activated Protein Kinase 8 - antagonists & inhibitors</topic><topic>Mitogen-Activated Protein Kinase 8 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 9 - analysis</topic><topic>Mitogen-Activated Protein Kinase 9 - metabolism</topic><topic>RNA, Messenger - agonists</topic><topic>RNA, Messenger - analysis</topic><topic>Serpins - genetics</topic><topic>Serpins - pharmacology</topic><topic>Serpins - physiology</topic><topic>Signal Transduction</topic><topic>Squamous cell carcinoma antigen</topic><topic>Ultraviolet irradiation</topic><topic>Ultraviolet Rays</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katagiri, Chika</creatorcontrib><creatorcontrib>Negishi, Kei</creatorcontrib><creatorcontrib>Hibino, Toshihiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dermatological science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katagiri, Chika</au><au>Negishi, Kei</au><au>Hibino, Toshihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>c-JUN N-terminal kinase-1 (JNK1) but not JNK2 or JNK3 is involved in UV signal transduction in human epidermis</atitle><jtitle>Journal of dermatological science</jtitle><addtitle>J Dermatol Sci</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>43</volume><issue>3</issue><spage>171</spage><epage>179</epage><pages>171-179</pages><issn>0923-1811</issn><eissn>1873-569X</eissn><abstract>c-Jun N-terminal kinase (JNK) plays a critical role in UV-induced apoptotic cell death. Although three isoforms are known in mammals, physiological roles of each isoform are still obscure. Furthermore, our recent findings show that serpin squamous cell carcinoma antigen (SCCA1) binds to JNK.
To determine which isoform is responsible for the UV signal transduction in human epidermis and whether SCCA1 is capable to regulate kinase activity of a specific isoform.
Immunohistochemical localization of each JNK isoform was investigated after UV irradiation
in vivo and
in vitro. Effect of recombinant SCCA1 on JNK kinase activity was also analyzed.
Immunostaining for JNK1, 2 and 3 demonstrated marked elevation of JNK1 in spinous to granular cells of UV-irradiated skin, whereas they were expressed weakly in upper epidermis of the sun-protected, buttock skin. In cultured keratinocytes, only JNK1 is translocated into nucleus after UV irradiation. JNK2, which localized in the cytoplasm, or JNK3, which was confined in nucleus, remained in the same compartment after UV irradiation. We confirmed that only JNK1 mRNA was up-regulated after UV irradiation in cultured keratinocytes. In addition, recombinant SCCA1 suppressed kinase activity of JNK1 but did not affect JNK2 or JNK3 kinase activity.
JNK1 is associated with UV signal transduction in human epidermis and SCCA1 is a suppressor of this process.</abstract><cop>Netherlands</cop><pub>Elsevier Ireland Ltd</pub><pmid>16824735</pmid><doi>10.1016/j.jdermsci.2006.05.008</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Active Transport, Cell Nucleus Adult Antigens, Neoplasm - genetics Antigens, Neoplasm - pharmacology Antigens, Neoplasm - physiology Apoptosis c-Jun N-terminal kinase Cell Nucleus - enzymology Cytoplasm - enzymology Epidermis - drug effects Epidermis - enzymology Epidermis - radiation effects Female Humans Male Middle Aged Mitogen-Activated Protein Kinase 10 - analysis Mitogen-Activated Protein Kinase 10 - metabolism Mitogen-Activated Protein Kinase 8 - analysis Mitogen-Activated Protein Kinase 8 - antagonists & inhibitors Mitogen-Activated Protein Kinase 8 - metabolism Mitogen-Activated Protein Kinase 9 - analysis Mitogen-Activated Protein Kinase 9 - metabolism RNA, Messenger - agonists RNA, Messenger - analysis Serpins - genetics Serpins - pharmacology Serpins - physiology Signal Transduction Squamous cell carcinoma antigen Ultraviolet irradiation Ultraviolet Rays Up-Regulation |
| title | c-JUN N-terminal kinase-1 (JNK1) but not JNK2 or JNK3 is involved in UV signal transduction in human epidermis |
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