A comparison between percutaneous and surgical transplantation of autologous skeletal myoblasts in a swine model of chronic myocardial infarction
Our aim was to compare the efficacy of surgical versus percutaneous administration of skeletal myoblasts (SkM) in a swine model of chronic myocardial infarction and to determine the mechanism(s) involved in their beneficial effect. Two months after induction of myocardial infarction (MI), Goettingen...
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| Veröffentlicht in: | Cardiovascular research 2006-09, Vol.71 (4), p.744-753 |
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| creator | GAVIRA, Juan José PEREZ-ILZARBE, Maitane PANIZO, Angel GARCIA DE JALON, José A MARTINEZ-CARO, Diego PROSPER, Felipe ABIZANDA, Gloria GARCIA-RODRIGUEZ, Alba ORBE, Josune PARAMO, José Antonio BELZUNCE, Miriam RABAGO, Gregorio BARBA, Joaquin HERREROS, Jesus |
| description | Our aim was to compare the efficacy of surgical versus percutaneous administration of skeletal myoblasts (SkM) in a swine model of chronic myocardial infarction and to determine the mechanism(s) involved in their beneficial effect.
Two months after induction of myocardial infarction (MI), Goettingen miniature pigs underwent autologous SkM transplant either by direct surgical injection (n=6) or percutaneous access and intramyocardial delivery under fluoroscopic and echocardiographic guidance (n=6). Control animals received media alone (n=4). Functional analysis was performed by 2D echocardiography. Myoblast engraftment, in vivo cell differentiation, vessel formation, fibrosis, and the ratio between collagen type I/III deposition were analyzed in the infarct (IA) and non-infarct area (NIA) by immunohistochemistry.
Animals received a median of 407.55+/-115x10(6) BrdU-labeled autologous SkM. Myoblast transplant was associated with a statistically significant increase in left ventricular ejection fraction (p |
| doi_str_mv | 10.1016/j.cardiores.2006.06.018 |
| format | Article |
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Two months after induction of myocardial infarction (MI), Goettingen miniature pigs underwent autologous SkM transplant either by direct surgical injection (n=6) or percutaneous access and intramyocardial delivery under fluoroscopic and echocardiographic guidance (n=6). Control animals received media alone (n=4). Functional analysis was performed by 2D echocardiography. Myoblast engraftment, in vivo cell differentiation, vessel formation, fibrosis, and the ratio between collagen type I/III deposition were analyzed in the infarct (IA) and non-infarct area (NIA) by immunohistochemistry.
Animals received a median of 407.55+/-115x10(6) BrdU-labeled autologous SkM. Myoblast transplant was associated with a statistically significant increase in left ventricular ejection fraction (p<0.01), increased vasculogenesis and decreased fibrosis (p<0.05), and reduced collagen type I/III ratio in the IA and NIA areas as compared with control animals. No differences were found between groups receiving SkM by percutaneous or surgical access.
Our results indicate that increased vasculogenesis and changes in matrix remodeling with decreased fibrosis are associated with the beneficial effect of SkM transplant in chronic MI. The equivalent benefit observed from surgical and percutaneous delivery has important clinical implications.</description><identifier>ISSN: 0008-6363</identifier><identifier>EISSN: 1755-3245</identifier><identifier>DOI: 10.1016/j.cardiores.2006.06.018</identifier><identifier>PMID: 16843451</identifier><identifier>CODEN: CVREAU</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Actins - analysis ; Animals ; Biological and medical sciences ; Biomarkers - analysis ; Cardiology. Vascular system ; Cell Differentiation ; Collagen - analysis ; Coronary heart disease ; Echocardiography ; Fibrosis ; Heart ; Image Processing, Computer-Assisted ; Immunohistochemistry ; Injections, Subcutaneous ; Medical sciences ; Models, Animal ; Myoblasts, Skeletal - transplantation ; Myocardial Infarction - pathology ; Myocardial Infarction - physiopathology ; Myocardial Infarction - surgery ; Myocarditis. Cardiomyopathies ; Myocardium - metabolism ; Myocardium - pathology ; Neovascularization, Physiologic ; Swine ; Transplantation, Autologous ; Treatment Outcome ; Ventricular Dysfunction, Left ; Ventricular Remodeling</subject><ispartof>Cardiovascular research, 2006-09, Vol.71 (4), p.744-753</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-97609e11c64aa1c865988fe681b2f4e65772f85917f28a03f23d2e04dbb2251a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18051310$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16843451$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GAVIRA, Juan José</creatorcontrib><creatorcontrib>PEREZ-ILZARBE, Maitane</creatorcontrib><creatorcontrib>PANIZO, Angel</creatorcontrib><creatorcontrib>GARCIA DE JALON, José A</creatorcontrib><creatorcontrib>MARTINEZ-CARO, Diego</creatorcontrib><creatorcontrib>PROSPER, Felipe</creatorcontrib><creatorcontrib>ABIZANDA, Gloria</creatorcontrib><creatorcontrib>GARCIA-RODRIGUEZ, Alba</creatorcontrib><creatorcontrib>ORBE, Josune</creatorcontrib><creatorcontrib>PARAMO, José Antonio</creatorcontrib><creatorcontrib>BELZUNCE, Miriam</creatorcontrib><creatorcontrib>RABAGO, Gregorio</creatorcontrib><creatorcontrib>BARBA, Joaquin</creatorcontrib><creatorcontrib>HERREROS, Jesus</creatorcontrib><title>A comparison between percutaneous and surgical transplantation of autologous skeletal myoblasts in a swine model of chronic myocardial infarction</title><title>Cardiovascular research</title><addtitle>Cardiovasc Res</addtitle><description>Our aim was to compare the efficacy of surgical versus percutaneous administration of skeletal myoblasts (SkM) in a swine model of chronic myocardial infarction and to determine the mechanism(s) involved in their beneficial effect.
Two months after induction of myocardial infarction (MI), Goettingen miniature pigs underwent autologous SkM transplant either by direct surgical injection (n=6) or percutaneous access and intramyocardial delivery under fluoroscopic and echocardiographic guidance (n=6). Control animals received media alone (n=4). Functional analysis was performed by 2D echocardiography. Myoblast engraftment, in vivo cell differentiation, vessel formation, fibrosis, and the ratio between collagen type I/III deposition were analyzed in the infarct (IA) and non-infarct area (NIA) by immunohistochemistry.
Animals received a median of 407.55+/-115x10(6) BrdU-labeled autologous SkM. Myoblast transplant was associated with a statistically significant increase in left ventricular ejection fraction (p<0.01), increased vasculogenesis and decreased fibrosis (p<0.05), and reduced collagen type I/III ratio in the IA and NIA areas as compared with control animals. No differences were found between groups receiving SkM by percutaneous or surgical access.
Our results indicate that increased vasculogenesis and changes in matrix remodeling with decreased fibrosis are associated with the beneficial effect of SkM transplant in chronic MI. The equivalent benefit observed from surgical and percutaneous delivery has important clinical implications.</description><subject>Actins - analysis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Cardiology. Vascular system</subject><subject>Cell Differentiation</subject><subject>Collagen - analysis</subject><subject>Coronary heart disease</subject><subject>Echocardiography</subject><subject>Fibrosis</subject><subject>Heart</subject><subject>Image Processing, Computer-Assisted</subject><subject>Immunohistochemistry</subject><subject>Injections, Subcutaneous</subject><subject>Medical sciences</subject><subject>Models, Animal</subject><subject>Myoblasts, Skeletal - transplantation</subject><subject>Myocardial Infarction - pathology</subject><subject>Myocardial Infarction - physiopathology</subject><subject>Myocardial Infarction - surgery</subject><subject>Myocarditis. Cardiomyopathies</subject><subject>Myocardium - metabolism</subject><subject>Myocardium - pathology</subject><subject>Neovascularization, Physiologic</subject><subject>Swine</subject><subject>Transplantation, Autologous</subject><subject>Treatment Outcome</subject><subject>Ventricular Dysfunction, Left</subject><subject>Ventricular Remodeling</subject><issn>0008-6363</issn><issn>1755-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1q3TAQhUVpaW5-XqHVpt35VrIsWV6GkP5AoJt0bcbyKNWtLbmSTMhj5I0r914SGBgGvjPDnEPIR872nHH15bA3EEcXIqZ9zZjab8X1G7LjrZSVqBv5luwYY7pSQokzcp7SoYxSts17csaVbkQj-Y48X1MT5gWiS8HTAfMjoqcLRrNm8BjWRMGPNK3xwRmYaI7g0zKBz5BdUQRLYc1hCg8bmv7ghLlg81MYJkg5Uecp0PToPNI5jDhtCvM7Bu_MRv1_owictxDNtvKSvLMwJbw69Qvy6-vt_c336u7ntx8313eVaaTKVdcq1iHnRjUA3GglO60tKs2H2jaoZNvWVsuOt7bWwIStxVgja8ZhqGvJQVyQz8e9Swx_V0y5n10yOE3Ht3ulW6F5xwvYHkETQ0oRbb9EN0N86jnrtzT6Q_-SRr-l0W_FdVF-OJ1YhxnHV93J_gJ8OgGQiru2mGtceuU0KxBn4h_s35nr</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>GAVIRA, Juan José</creator><creator>PEREZ-ILZARBE, Maitane</creator><creator>PANIZO, Angel</creator><creator>GARCIA DE JALON, José A</creator><creator>MARTINEZ-CARO, Diego</creator><creator>PROSPER, Felipe</creator><creator>ABIZANDA, Gloria</creator><creator>GARCIA-RODRIGUEZ, Alba</creator><creator>ORBE, Josune</creator><creator>PARAMO, José Antonio</creator><creator>BELZUNCE, Miriam</creator><creator>RABAGO, Gregorio</creator><creator>BARBA, Joaquin</creator><creator>HERREROS, Jesus</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>A comparison between percutaneous and surgical transplantation of autologous skeletal myoblasts in a swine model of chronic myocardial infarction</title><author>GAVIRA, Juan José ; PEREZ-ILZARBE, Maitane ; PANIZO, Angel ; GARCIA DE JALON, José A ; MARTINEZ-CARO, Diego ; PROSPER, Felipe ; ABIZANDA, Gloria ; GARCIA-RODRIGUEZ, Alba ; ORBE, Josune ; PARAMO, José Antonio ; BELZUNCE, Miriam ; RABAGO, Gregorio ; BARBA, Joaquin ; HERREROS, Jesus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-97609e11c64aa1c865988fe681b2f4e65772f85917f28a03f23d2e04dbb2251a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Actins - analysis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Cardiology. Vascular system</topic><topic>Cell Differentiation</topic><topic>Collagen - analysis</topic><topic>Coronary heart disease</topic><topic>Echocardiography</topic><topic>Fibrosis</topic><topic>Heart</topic><topic>Image Processing, Computer-Assisted</topic><topic>Immunohistochemistry</topic><topic>Injections, Subcutaneous</topic><topic>Medical sciences</topic><topic>Models, Animal</topic><topic>Myoblasts, Skeletal - transplantation</topic><topic>Myocardial Infarction - pathology</topic><topic>Myocardial Infarction - physiopathology</topic><topic>Myocardial Infarction - surgery</topic><topic>Myocarditis. Cardiomyopathies</topic><topic>Myocardium - metabolism</topic><topic>Myocardium - pathology</topic><topic>Neovascularization, Physiologic</topic><topic>Swine</topic><topic>Transplantation, Autologous</topic><topic>Treatment Outcome</topic><topic>Ventricular Dysfunction, Left</topic><topic>Ventricular Remodeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GAVIRA, Juan José</creatorcontrib><creatorcontrib>PEREZ-ILZARBE, Maitane</creatorcontrib><creatorcontrib>PANIZO, Angel</creatorcontrib><creatorcontrib>GARCIA DE JALON, José A</creatorcontrib><creatorcontrib>MARTINEZ-CARO, Diego</creatorcontrib><creatorcontrib>PROSPER, Felipe</creatorcontrib><creatorcontrib>ABIZANDA, Gloria</creatorcontrib><creatorcontrib>GARCIA-RODRIGUEZ, Alba</creatorcontrib><creatorcontrib>ORBE, Josune</creatorcontrib><creatorcontrib>PARAMO, José Antonio</creatorcontrib><creatorcontrib>BELZUNCE, Miriam</creatorcontrib><creatorcontrib>RABAGO, Gregorio</creatorcontrib><creatorcontrib>BARBA, Joaquin</creatorcontrib><creatorcontrib>HERREROS, Jesus</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cardiovascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GAVIRA, Juan José</au><au>PEREZ-ILZARBE, Maitane</au><au>PANIZO, Angel</au><au>GARCIA DE JALON, José A</au><au>MARTINEZ-CARO, Diego</au><au>PROSPER, Felipe</au><au>ABIZANDA, Gloria</au><au>GARCIA-RODRIGUEZ, Alba</au><au>ORBE, Josune</au><au>PARAMO, José Antonio</au><au>BELZUNCE, Miriam</au><au>RABAGO, Gregorio</au><au>BARBA, Joaquin</au><au>HERREROS, Jesus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison between percutaneous and surgical transplantation of autologous skeletal myoblasts in a swine model of chronic myocardial infarction</atitle><jtitle>Cardiovascular research</jtitle><addtitle>Cardiovasc Res</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>71</volume><issue>4</issue><spage>744</spage><epage>753</epage><pages>744-753</pages><issn>0008-6363</issn><eissn>1755-3245</eissn><coden>CVREAU</coden><abstract>Our aim was to compare the efficacy of surgical versus percutaneous administration of skeletal myoblasts (SkM) in a swine model of chronic myocardial infarction and to determine the mechanism(s) involved in their beneficial effect.
Two months after induction of myocardial infarction (MI), Goettingen miniature pigs underwent autologous SkM transplant either by direct surgical injection (n=6) or percutaneous access and intramyocardial delivery under fluoroscopic and echocardiographic guidance (n=6). Control animals received media alone (n=4). Functional analysis was performed by 2D echocardiography. Myoblast engraftment, in vivo cell differentiation, vessel formation, fibrosis, and the ratio between collagen type I/III deposition were analyzed in the infarct (IA) and non-infarct area (NIA) by immunohistochemistry.
Animals received a median of 407.55+/-115x10(6) BrdU-labeled autologous SkM. Myoblast transplant was associated with a statistically significant increase in left ventricular ejection fraction (p<0.01), increased vasculogenesis and decreased fibrosis (p<0.05), and reduced collagen type I/III ratio in the IA and NIA areas as compared with control animals. No differences were found between groups receiving SkM by percutaneous or surgical access.
Our results indicate that increased vasculogenesis and changes in matrix remodeling with decreased fibrosis are associated with the beneficial effect of SkM transplant in chronic MI. The equivalent benefit observed from surgical and percutaneous delivery has important clinical implications.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>16843451</pmid><doi>10.1016/j.cardiores.2006.06.018</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Actins - analysis Animals Biological and medical sciences Biomarkers - analysis Cardiology. Vascular system Cell Differentiation Collagen - analysis Coronary heart disease Echocardiography Fibrosis Heart Image Processing, Computer-Assisted Immunohistochemistry Injections, Subcutaneous Medical sciences Models, Animal Myoblasts, Skeletal - transplantation Myocardial Infarction - pathology Myocardial Infarction - physiopathology Myocardial Infarction - surgery Myocarditis. Cardiomyopathies Myocardium - metabolism Myocardium - pathology Neovascularization, Physiologic Swine Transplantation, Autologous Treatment Outcome Ventricular Dysfunction, Left Ventricular Remodeling |
| title | A comparison between percutaneous and surgical transplantation of autologous skeletal myoblasts in a swine model of chronic myocardial infarction |
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