Age-related alterations to immune parameters in Labrador retriever dogs
In order to assess age-related changes in the immune status of Labrador retriever dogs, leukocyte phenotypes, lymphocyte proliferative capacity, and serum antibody levels were measured in four cohorts of dogs, ranging from 2 to 10 years of age. Absolute numbers of white blood cells, lymphocytes, mon...
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| Veröffentlicht in: | Veterinary Immunology and Immunopathology 2005-12, Vol.108 (3), p.399-407 |
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| creator | Blount, Daniel G. Pritchard, David I. Heaton, Paul R. |
| description | In order to assess age-related changes in the immune status of Labrador retriever dogs, leukocyte phenotypes, lymphocyte proliferative capacity, and serum antibody levels were measured in four cohorts of dogs, ranging from 2 to 10 years of age. Absolute numbers of white blood cells, lymphocytes, monocytes, granulocytes, and CD3+, CD4+, CD8+ and CD21+ lymphocytes significantly decreased with increasing age. Relative percentages of lymphocytes and CD4 cells were significantly decreased, and relative percentages of granulocytes and CD8 cells significantly increased, with age. The CD4:CD8 ratio showed a significant age-related decrease. Proliferative responses of T-cells to mitogens in whole-blood cultures either increased (Concanavalin A) or remained the same (phytohemagglutinin) with age when data was normalised to allow for differences in responding cell number. Similarly, normalised data of proliferative response to anti-CD3 stimulation together with phorbol 12-myristate 13-acetate showed an age-related increase. Serum levels of total IgA significantly increased with age whereas total IgG levels remained unchanged.
These observations illustrate a significant change to a number of immune parameters with age. However, further work is required to determine whether the differences reported here are sufficient to cause overt or functional immune senescence in Labrador retriever dogs. |
| doi_str_mv | 10.1016/j.vetimm.2005.06.015 |
| format | Article |
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These observations illustrate a significant change to a number of immune parameters with age. However, further work is required to determine whether the differences reported here are sufficient to cause overt or functional immune senescence in Labrador retriever dogs.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/j.vetimm.2005.06.015</identifier><identifier>PMID: 16105688</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Ageing ; Aging - blood ; Aging - immunology ; animal age ; Animals ; antibodies ; Canine ; CD4-positive T-lymphocytes ; CD8-positive T-lymphocytes ; Cell Proliferation ; concanavalin A ; dogs ; Dogs - blood ; Dogs - classification ; Dogs - immunology ; Female ; granulocytes ; Immune senescence ; Immunoglobulin ; immunoglobulin A ; Immunoglobulin A - blood ; immunoglobulin G ; Immunoglobulin G - blood ; Labrador Retriever ; leukocyte count ; Leukocytes - cytology ; Leukocytes - immunology ; Lymphocyte ; lymphocyte count ; lymphocyte proliferation ; Male ; monocytes ; Phenotype ; phytohemagglutinin ; Proliferation ; senescence</subject><ispartof>Veterinary Immunology and Immunopathology, 2005-12, Vol.108 (3), p.399-407</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-10bd6b46c4e12075a1a9ae08c1e524ddebd14de35687526353faa028dccaec763</citedby><cites>FETCH-LOGICAL-c481t-10bd6b46c4e12075a1a9ae08c1e524ddebd14de35687526353faa028dccaec763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetimm.2005.06.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16105688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blount, Daniel G.</creatorcontrib><creatorcontrib>Pritchard, David I.</creatorcontrib><creatorcontrib>Heaton, Paul R.</creatorcontrib><title>Age-related alterations to immune parameters in Labrador retriever dogs</title><title>Veterinary Immunology and Immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>In order to assess age-related changes in the immune status of Labrador retriever dogs, leukocyte phenotypes, lymphocyte proliferative capacity, and serum antibody levels were measured in four cohorts of dogs, ranging from 2 to 10 years of age. Absolute numbers of white blood cells, lymphocytes, monocytes, granulocytes, and CD3+, CD4+, CD8+ and CD21+ lymphocytes significantly decreased with increasing age. Relative percentages of lymphocytes and CD4 cells were significantly decreased, and relative percentages of granulocytes and CD8 cells significantly increased, with age. The CD4:CD8 ratio showed a significant age-related decrease. Proliferative responses of T-cells to mitogens in whole-blood cultures either increased (Concanavalin A) or remained the same (phytohemagglutinin) with age when data was normalised to allow for differences in responding cell number. Similarly, normalised data of proliferative response to anti-CD3 stimulation together with phorbol 12-myristate 13-acetate showed an age-related increase. Serum levels of total IgA significantly increased with age whereas total IgG levels remained unchanged.
These observations illustrate a significant change to a number of immune parameters with age. However, further work is required to determine whether the differences reported here are sufficient to cause overt or functional immune senescence in Labrador retriever dogs.</description><subject>Ageing</subject><subject>Aging - blood</subject><subject>Aging - immunology</subject><subject>animal age</subject><subject>Animals</subject><subject>antibodies</subject><subject>Canine</subject><subject>CD4-positive T-lymphocytes</subject><subject>CD8-positive T-lymphocytes</subject><subject>Cell Proliferation</subject><subject>concanavalin A</subject><subject>dogs</subject><subject>Dogs - blood</subject><subject>Dogs - classification</subject><subject>Dogs - immunology</subject><subject>Female</subject><subject>granulocytes</subject><subject>Immune senescence</subject><subject>Immunoglobulin</subject><subject>immunoglobulin A</subject><subject>Immunoglobulin A - blood</subject><subject>immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Labrador Retriever</subject><subject>leukocyte count</subject><subject>Leukocytes - cytology</subject><subject>Leukocytes - immunology</subject><subject>Lymphocyte</subject><subject>lymphocyte count</subject><subject>lymphocyte proliferation</subject><subject>Male</subject><subject>monocytes</subject><subject>Phenotype</subject><subject>phytohemagglutinin</subject><subject>Proliferation</subject><subject>senescence</subject><issn>0165-2427</issn><issn>1873-2534</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rGzEURUVpaZyk_6C0s8pupnoaSSNvCiE0ScHQRZO1eCM9G5n5cCXZ0H9fmTF0167e4p17uRzGPgJvgIP-sm9OlMM4NoJz1XDdcFBv2ApM19ZCtfItWxVM1UKK7opdp7TnBVwb855dgQautDEr9nS_ozrSgJl8hUOmiDnMU6ryXJXy40TVASOOVD6pClO1wT6in2MVKcdAJ4qVn3fplr3b4pDow-XesNfHby8Pz_Xmx9P3h_tN7aSBXAPvve6ldpJA8E4h4BqJGwekhPSeeg_SU1vGdUroVrVbRC6Mdw7Jdbq9YXdL7yHOv46Ush1DcjQMONF8TLbkxFqb9X9B6CRvlVEFlAvo4pxSpK09xDBi_G2B27Npu7eLaXs2bbm2xXSJfbr0H_uR_N_QRW0BPi_AFmeLuxiSff0pOLQcuBQSzhO_LgQVYadA0SYXaHLkQySXrZ_Dvzf8Ab3Emso</recordid><startdate>20051215</startdate><enddate>20051215</enddate><creator>Blount, Daniel G.</creator><creator>Pritchard, David I.</creator><creator>Heaton, Paul R.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20051215</creationdate><title>Age-related alterations to immune parameters in Labrador retriever dogs</title><author>Blount, Daniel G. ; Pritchard, David I. ; Heaton, Paul R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-10bd6b46c4e12075a1a9ae08c1e524ddebd14de35687526353faa028dccaec763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Ageing</topic><topic>Aging - blood</topic><topic>Aging - immunology</topic><topic>animal age</topic><topic>Animals</topic><topic>antibodies</topic><topic>Canine</topic><topic>CD4-positive T-lymphocytes</topic><topic>CD8-positive T-lymphocytes</topic><topic>Cell Proliferation</topic><topic>concanavalin A</topic><topic>dogs</topic><topic>Dogs - blood</topic><topic>Dogs - classification</topic><topic>Dogs - immunology</topic><topic>Female</topic><topic>granulocytes</topic><topic>Immune senescence</topic><topic>Immunoglobulin</topic><topic>immunoglobulin A</topic><topic>Immunoglobulin A - blood</topic><topic>immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>Labrador Retriever</topic><topic>leukocyte count</topic><topic>Leukocytes - cytology</topic><topic>Leukocytes - immunology</topic><topic>Lymphocyte</topic><topic>lymphocyte count</topic><topic>lymphocyte proliferation</topic><topic>Male</topic><topic>monocytes</topic><topic>Phenotype</topic><topic>phytohemagglutinin</topic><topic>Proliferation</topic><topic>senescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blount, Daniel G.</creatorcontrib><creatorcontrib>Pritchard, David I.</creatorcontrib><creatorcontrib>Heaton, Paul R.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary Immunology and Immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blount, Daniel G.</au><au>Pritchard, David I.</au><au>Heaton, Paul R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-related alterations to immune parameters in Labrador retriever dogs</atitle><jtitle>Veterinary Immunology and Immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2005-12-15</date><risdate>2005</risdate><volume>108</volume><issue>3</issue><spage>399</spage><epage>407</epage><pages>399-407</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><eissn>1365-2567</eissn><abstract>In order to assess age-related changes in the immune status of Labrador retriever dogs, leukocyte phenotypes, lymphocyte proliferative capacity, and serum antibody levels were measured in four cohorts of dogs, ranging from 2 to 10 years of age. Absolute numbers of white blood cells, lymphocytes, monocytes, granulocytes, and CD3+, CD4+, CD8+ and CD21+ lymphocytes significantly decreased with increasing age. Relative percentages of lymphocytes and CD4 cells were significantly decreased, and relative percentages of granulocytes and CD8 cells significantly increased, with age. The CD4:CD8 ratio showed a significant age-related decrease. Proliferative responses of T-cells to mitogens in whole-blood cultures either increased (Concanavalin A) or remained the same (phytohemagglutinin) with age when data was normalised to allow for differences in responding cell number. Similarly, normalised data of proliferative response to anti-CD3 stimulation together with phorbol 12-myristate 13-acetate showed an age-related increase. Serum levels of total IgA significantly increased with age whereas total IgG levels remained unchanged.
These observations illustrate a significant change to a number of immune parameters with age. However, further work is required to determine whether the differences reported here are sufficient to cause overt or functional immune senescence in Labrador retriever dogs.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16105688</pmid><doi>10.1016/j.vetimm.2005.06.015</doi><tpages>9</tpages></addata></record> |
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| subjects | Ageing Aging - blood Aging - immunology animal age Animals antibodies Canine CD4-positive T-lymphocytes CD8-positive T-lymphocytes Cell Proliferation concanavalin A dogs Dogs - blood Dogs - classification Dogs - immunology Female granulocytes Immune senescence Immunoglobulin immunoglobulin A Immunoglobulin A - blood immunoglobulin G Immunoglobulin G - blood Labrador Retriever leukocyte count Leukocytes - cytology Leukocytes - immunology Lymphocyte lymphocyte count lymphocyte proliferation Male monocytes Phenotype phytohemagglutinin Proliferation senescence |
| title | Age-related alterations to immune parameters in Labrador retriever dogs |
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