Relevance of Urine Telomerase in the Diagnosis of Bladder Cancer
CONTEXT The identification of new molecular markers is one of the most challenging goals for the early detection of bladder cancer because available noninvasive methods have neither sufficient sensitivity nor specificity to be acceptable for routine use. OBJECTIVE To develop a relatively simple, ine...
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| Veröffentlicht in: | JAMA : the journal of the American Medical Association 2005-10, Vol.294 (16), p.2052-2056 |
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| container_title | JAMA : the journal of the American Medical Association |
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| creator | Sanchini, Maria Aurora Gunelli, Roberta Nanni, Oriana Bravaccini, Sara Fabbri, Carla Sermasi, Alice Bercovich, Eduard Ravaioli, Alberto Amadori, Dino Calistri, Daniele |
| description | CONTEXT The identification of new molecular markers is one of the most challenging
goals for the early detection of bladder cancer because available noninvasive
methods have neither sufficient sensitivity nor specificity to be acceptable
for routine use. OBJECTIVE To develop a relatively simple, inexpensive, and accurate test that
measures telomerase activity in voided urine to apply to large-scale screening
programs for bladder cancer detection. DESIGN, SETTING, AND PARTICIPANTS Case-control study conducted in 218 men (84 healthy individuals and
134 patients at first diagnosis of histologically confirmed bladder cancer),
frequency matched by age and recruited between March 2003 and November 2004
in Italy. Urine telomerase activity was determined using a highly sensitive
telomeric repeat amplification protocol (TRAP) assay. Urine samples were processed
for cytological diagnosis and TRAP assay. The diagnosis of bladder cancer
was based on bioptic and cystoscopic examinations. The performance of the
TRAP assay to detect urine telomerase activity was compared with urine cytology
as an aid to early cancer detection. Quantification of urine telomerase activity
was conducted in a blinded manner. MAIN OUTCOME MEASURE Sensitivity and specificity of TRAP to detect bladder cancer. RESULTS Using a 50 arbitrary enzymatic unit cutoff value, we validated the results
obtained in the pilot study. In the overall series, sensitivity was 90% (95%
confidence interval [CI], 83%-94%) and specificity was 88% (95% CI, 79%-93%).
Specificity increased to 94% (95% CI, 85%-98%) for individuals aged 75 years
or younger. The same predictive capacity of telomerase activity levels was
observed for patients with low-grade tumors or with negative cytology results. CONCLUSIONS The present validation study demonstrated the ability of urine telomerase
activity levels to accurately detect the presence of bladder tumors in men.
This test represents a potentially useful noninvasive diagnostic innovation
for bladder cancer detection in high-risk groups such as habitual smokers
or in symptomatic patients. |
| doi_str_mv | 10.1001/jama.294.16.2052 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_68729009</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><ama_id>201756</ama_id><sourcerecordid>17397282</sourcerecordid><originalsourceid>FETCH-LOGICAL-a362t-cbae9d0b4a4e02473c709f8582b7d28ed9fd4490a7cd505e260afeaad3e203603</originalsourceid><addsrcrecordid>eNqF0UtLw0AQAOBFFFurd71IEPSWuK_s46bWJxQEac9hkp1oSh51txX896a0KnhxLnuYb2eYGUKOGU0YpexyDg0k3MqEqYTTlO-QIUuFiUVqzS4ZUmpNrKWRA3IQwpz2wYTeJwOmuLSS2SG5esEaP6AtMOrKaOarFqMp1l2DHgJGVRst3zC6reC17UIV1uimBufQR-P1L39I9kqoAx5t3xGZ3d9Nx4_x5PnhaXw9iUEovoyLHNA6mkuQSLnUotDUliY1PNeOG3S2dFJaCrpwKU2RKwolAjiBnApFxYhcbOoufPe-wrDMmioUWNfQYrcKmTKa237gfyHTwmpueA_P_sB5t_JtP0TGGRPKGr6udrpFq7xBly181YD_zL432IPzLYBQQF36fitV-HWaWWXVutvJxvUn-8lyynSqxBfVWoY1</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>211369829</pqid></control><display><type>article</type><title>Relevance of Urine Telomerase in the Diagnosis of Bladder Cancer</title><source>MEDLINE</source><source>American Medical Association Journals (including JAMA)</source><creator>Sanchini, Maria Aurora ; Gunelli, Roberta ; Nanni, Oriana ; Bravaccini, Sara ; Fabbri, Carla ; Sermasi, Alice ; Bercovich, Eduard ; Ravaioli, Alberto ; Amadori, Dino ; Calistri, Daniele</creator><creatorcontrib>Sanchini, Maria Aurora ; Gunelli, Roberta ; Nanni, Oriana ; Bravaccini, Sara ; Fabbri, Carla ; Sermasi, Alice ; Bercovich, Eduard ; Ravaioli, Alberto ; Amadori, Dino ; Calistri, Daniele</creatorcontrib><description>CONTEXT The identification of new molecular markers is one of the most challenging
goals for the early detection of bladder cancer because available noninvasive
methods have neither sufficient sensitivity nor specificity to be acceptable
for routine use. OBJECTIVE To develop a relatively simple, inexpensive, and accurate test that
measures telomerase activity in voided urine to apply to large-scale screening
programs for bladder cancer detection. DESIGN, SETTING, AND PARTICIPANTS Case-control study conducted in 218 men (84 healthy individuals and
134 patients at first diagnosis of histologically confirmed bladder cancer),
frequency matched by age and recruited between March 2003 and November 2004
in Italy. Urine telomerase activity was determined using a highly sensitive
telomeric repeat amplification protocol (TRAP) assay. Urine samples were processed
for cytological diagnosis and TRAP assay. The diagnosis of bladder cancer
was based on bioptic and cystoscopic examinations. The performance of the
TRAP assay to detect urine telomerase activity was compared with urine cytology
as an aid to early cancer detection. Quantification of urine telomerase activity
was conducted in a blinded manner. MAIN OUTCOME MEASURE Sensitivity and specificity of TRAP to detect bladder cancer. RESULTS Using a 50 arbitrary enzymatic unit cutoff value, we validated the results
obtained in the pilot study. In the overall series, sensitivity was 90% (95%
confidence interval [CI], 83%-94%) and specificity was 88% (95% CI, 79%-93%).
Specificity increased to 94% (95% CI, 85%-98%) for individuals aged 75 years
or younger. The same predictive capacity of telomerase activity levels was
observed for patients with low-grade tumors or with negative cytology results. CONCLUSIONS The present validation study demonstrated the ability of urine telomerase
activity levels to accurately detect the presence of bladder tumors in men.
This test represents a potentially useful noninvasive diagnostic innovation
for bladder cancer detection in high-risk groups such as habitual smokers
or in symptomatic patients.</description><identifier>ISSN: 0098-7484</identifier><identifier>EISSN: 1538-3598</identifier><identifier>DOI: 10.1001/jama.294.16.2052</identifier><identifier>PMID: 16249419</identifier><identifier>CODEN: JAMAAP</identifier><language>eng</language><publisher>Chicago, IL: American Medical Association</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Biomarkers, Tumor - urine ; Bladder cancer ; Cancer ; Case-Control Studies ; Excretory system ; General aspects ; Humans ; Male ; Mass Screening ; Medical diagnosis ; Medical sciences ; Middle Aged ; Nephrology. Urinary tract diseases ; Nucleic Acid Amplification Techniques ; Sensitivity and Specificity ; Telomerase - urine ; Tumors of the urinary system ; Urinary Bladder Neoplasms - diagnosis ; Urinary Bladder Neoplasms - enzymology ; Urinary Bladder Neoplasms - urine ; Urinary system involvement in other diseases. Miscellaneous ; Urinary tract. Prostate gland ; Urine ; Urine - cytology</subject><ispartof>JAMA : the journal of the American Medical Association, 2005-10, Vol.294 (16), p.2052-2056</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright American Medical Association Oct 26, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a362t-cbae9d0b4a4e02473c709f8582b7d28ed9fd4490a7cd505e260afeaad3e203603</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://jamanetwork.com/journals/jama/articlepdf/10.1001/jama.294.16.2052$$EPDF$$P50$$Gama$$H</linktopdf><linktohtml>$$Uhttps://jamanetwork.com/journals/jama/fullarticle/10.1001/jama.294.16.2052$$EHTML$$P50$$Gama$$H</linktohtml><link.rule.ids>64,314,776,780,3327,27901,27902,76231,76234</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17196962$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16249419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sanchini, Maria Aurora</creatorcontrib><creatorcontrib>Gunelli, Roberta</creatorcontrib><creatorcontrib>Nanni, Oriana</creatorcontrib><creatorcontrib>Bravaccini, Sara</creatorcontrib><creatorcontrib>Fabbri, Carla</creatorcontrib><creatorcontrib>Sermasi, Alice</creatorcontrib><creatorcontrib>Bercovich, Eduard</creatorcontrib><creatorcontrib>Ravaioli, Alberto</creatorcontrib><creatorcontrib>Amadori, Dino</creatorcontrib><creatorcontrib>Calistri, Daniele</creatorcontrib><title>Relevance of Urine Telomerase in the Diagnosis of Bladder Cancer</title><title>JAMA : the journal of the American Medical Association</title><addtitle>JAMA</addtitle><description>CONTEXT The identification of new molecular markers is one of the most challenging
goals for the early detection of bladder cancer because available noninvasive
methods have neither sufficient sensitivity nor specificity to be acceptable
for routine use. OBJECTIVE To develop a relatively simple, inexpensive, and accurate test that
measures telomerase activity in voided urine to apply to large-scale screening
programs for bladder cancer detection. DESIGN, SETTING, AND PARTICIPANTS Case-control study conducted in 218 men (84 healthy individuals and
134 patients at first diagnosis of histologically confirmed bladder cancer),
frequency matched by age and recruited between March 2003 and November 2004
in Italy. Urine telomerase activity was determined using a highly sensitive
telomeric repeat amplification protocol (TRAP) assay. Urine samples were processed
for cytological diagnosis and TRAP assay. The diagnosis of bladder cancer
was based on bioptic and cystoscopic examinations. The performance of the
TRAP assay to detect urine telomerase activity was compared with urine cytology
as an aid to early cancer detection. Quantification of urine telomerase activity
was conducted in a blinded manner. MAIN OUTCOME MEASURE Sensitivity and specificity of TRAP to detect bladder cancer. RESULTS Using a 50 arbitrary enzymatic unit cutoff value, we validated the results
obtained in the pilot study. In the overall series, sensitivity was 90% (95%
confidence interval [CI], 83%-94%) and specificity was 88% (95% CI, 79%-93%).
Specificity increased to 94% (95% CI, 85%-98%) for individuals aged 75 years
or younger. The same predictive capacity of telomerase activity levels was
observed for patients with low-grade tumors or with negative cytology results. CONCLUSIONS The present validation study demonstrated the ability of urine telomerase
activity levels to accurately detect the presence of bladder tumors in men.
This test represents a potentially useful noninvasive diagnostic innovation
for bladder cancer detection in high-risk groups such as habitual smokers
or in symptomatic patients.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - urine</subject><subject>Bladder cancer</subject><subject>Cancer</subject><subject>Case-Control Studies</subject><subject>Excretory system</subject><subject>General aspects</subject><subject>Humans</subject><subject>Male</subject><subject>Mass Screening</subject><subject>Medical diagnosis</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Sensitivity and Specificity</subject><subject>Telomerase - urine</subject><subject>Tumors of the urinary system</subject><subject>Urinary Bladder Neoplasms - diagnosis</subject><subject>Urinary Bladder Neoplasms - enzymology</subject><subject>Urinary Bladder Neoplasms - urine</subject><subject>Urinary system involvement in other diseases. Miscellaneous</subject><subject>Urinary tract. Prostate gland</subject><subject>Urine</subject><subject>Urine - cytology</subject><issn>0098-7484</issn><issn>1538-3598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UtLw0AQAOBFFFurd71IEPSWuK_s46bWJxQEac9hkp1oSh51txX896a0KnhxLnuYb2eYGUKOGU0YpexyDg0k3MqEqYTTlO-QIUuFiUVqzS4ZUmpNrKWRA3IQwpz2wYTeJwOmuLSS2SG5esEaP6AtMOrKaOarFqMp1l2DHgJGVRst3zC6reC17UIV1uimBufQR-P1L39I9kqoAx5t3xGZ3d9Nx4_x5PnhaXw9iUEovoyLHNA6mkuQSLnUotDUliY1PNeOG3S2dFJaCrpwKU2RKwolAjiBnApFxYhcbOoufPe-wrDMmioUWNfQYrcKmTKa237gfyHTwmpueA_P_sB5t_JtP0TGGRPKGr6udrpFq7xBly181YD_zL432IPzLYBQQF36fitV-HWaWWXVutvJxvUn-8lyynSqxBfVWoY1</recordid><startdate>20051026</startdate><enddate>20051026</enddate><creator>Sanchini, Maria Aurora</creator><creator>Gunelli, Roberta</creator><creator>Nanni, Oriana</creator><creator>Bravaccini, Sara</creator><creator>Fabbri, Carla</creator><creator>Sermasi, Alice</creator><creator>Bercovich, Eduard</creator><creator>Ravaioli, Alberto</creator><creator>Amadori, Dino</creator><creator>Calistri, Daniele</creator><general>American Medical Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7QP</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20051026</creationdate><title>Relevance of Urine Telomerase in the Diagnosis of Bladder Cancer</title><author>Sanchini, Maria Aurora ; Gunelli, Roberta ; Nanni, Oriana ; Bravaccini, Sara ; Fabbri, Carla ; Sermasi, Alice ; Bercovich, Eduard ; Ravaioli, Alberto ; Amadori, Dino ; Calistri, Daniele</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a362t-cbae9d0b4a4e02473c709f8582b7d28ed9fd4490a7cd505e260afeaad3e203603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - urine</topic><topic>Bladder cancer</topic><topic>Cancer</topic><topic>Case-Control Studies</topic><topic>Excretory system</topic><topic>General aspects</topic><topic>Humans</topic><topic>Male</topic><topic>Mass Screening</topic><topic>Medical diagnosis</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Sensitivity and Specificity</topic><topic>Telomerase - urine</topic><topic>Tumors of the urinary system</topic><topic>Urinary Bladder Neoplasms - diagnosis</topic><topic>Urinary Bladder Neoplasms - enzymology</topic><topic>Urinary Bladder Neoplasms - urine</topic><topic>Urinary system involvement in other diseases. Miscellaneous</topic><topic>Urinary tract. Prostate gland</topic><topic>Urine</topic><topic>Urine - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanchini, Maria Aurora</creatorcontrib><creatorcontrib>Gunelli, Roberta</creatorcontrib><creatorcontrib>Nanni, Oriana</creatorcontrib><creatorcontrib>Bravaccini, Sara</creatorcontrib><creatorcontrib>Fabbri, Carla</creatorcontrib><creatorcontrib>Sermasi, Alice</creatorcontrib><creatorcontrib>Bercovich, Eduard</creatorcontrib><creatorcontrib>Ravaioli, Alberto</creatorcontrib><creatorcontrib>Amadori, Dino</creatorcontrib><creatorcontrib>Calistri, Daniele</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>JAMA : the journal of the American Medical Association</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanchini, Maria Aurora</au><au>Gunelli, Roberta</au><au>Nanni, Oriana</au><au>Bravaccini, Sara</au><au>Fabbri, Carla</au><au>Sermasi, Alice</au><au>Bercovich, Eduard</au><au>Ravaioli, Alberto</au><au>Amadori, Dino</au><au>Calistri, Daniele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relevance of Urine Telomerase in the Diagnosis of Bladder Cancer</atitle><jtitle>JAMA : the journal of the American Medical Association</jtitle><addtitle>JAMA</addtitle><date>2005-10-26</date><risdate>2005</risdate><volume>294</volume><issue>16</issue><spage>2052</spage><epage>2056</epage><pages>2052-2056</pages><issn>0098-7484</issn><eissn>1538-3598</eissn><coden>JAMAAP</coden><abstract>CONTEXT The identification of new molecular markers is one of the most challenging
goals for the early detection of bladder cancer because available noninvasive
methods have neither sufficient sensitivity nor specificity to be acceptable
for routine use. OBJECTIVE To develop a relatively simple, inexpensive, and accurate test that
measures telomerase activity in voided urine to apply to large-scale screening
programs for bladder cancer detection. DESIGN, SETTING, AND PARTICIPANTS Case-control study conducted in 218 men (84 healthy individuals and
134 patients at first diagnosis of histologically confirmed bladder cancer),
frequency matched by age and recruited between March 2003 and November 2004
in Italy. Urine telomerase activity was determined using a highly sensitive
telomeric repeat amplification protocol (TRAP) assay. Urine samples were processed
for cytological diagnosis and TRAP assay. The diagnosis of bladder cancer
was based on bioptic and cystoscopic examinations. The performance of the
TRAP assay to detect urine telomerase activity was compared with urine cytology
as an aid to early cancer detection. Quantification of urine telomerase activity
was conducted in a blinded manner. MAIN OUTCOME MEASURE Sensitivity and specificity of TRAP to detect bladder cancer. RESULTS Using a 50 arbitrary enzymatic unit cutoff value, we validated the results
obtained in the pilot study. In the overall series, sensitivity was 90% (95%
confidence interval [CI], 83%-94%) and specificity was 88% (95% CI, 79%-93%).
Specificity increased to 94% (95% CI, 85%-98%) for individuals aged 75 years
or younger. The same predictive capacity of telomerase activity levels was
observed for patients with low-grade tumors or with negative cytology results. CONCLUSIONS The present validation study demonstrated the ability of urine telomerase
activity levels to accurately detect the presence of bladder tumors in men.
This test represents a potentially useful noninvasive diagnostic innovation
for bladder cancer detection in high-risk groups such as habitual smokers
or in symptomatic patients.</abstract><cop>Chicago, IL</cop><pub>American Medical Association</pub><pmid>16249419</pmid><doi>10.1001/jama.294.16.2052</doi><tpages>5</tpages></addata></record> |
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| ispartof | JAMA : the journal of the American Medical Association, 2005-10, Vol.294 (16), p.2052-2056 |
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| source | MEDLINE; American Medical Association Journals (including JAMA) |
| subjects | Adult Aged Aged, 80 and over Biological and medical sciences Biomarkers, Tumor - urine Bladder cancer Cancer Case-Control Studies Excretory system General aspects Humans Male Mass Screening Medical diagnosis Medical sciences Middle Aged Nephrology. Urinary tract diseases Nucleic Acid Amplification Techniques Sensitivity and Specificity Telomerase - urine Tumors of the urinary system Urinary Bladder Neoplasms - diagnosis Urinary Bladder Neoplasms - enzymology Urinary Bladder Neoplasms - urine Urinary system involvement in other diseases. Miscellaneous Urinary tract. Prostate gland Urine Urine - cytology |
| title | Relevance of Urine Telomerase in the Diagnosis of Bladder Cancer |
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