Effective method for in-vitro culture of cryopreserved human ovarian tissue
It is widely accepted that it is possible to successfully cryopreserve human ovarian tissue by direct plunging into liquid nitrogen using permeable cryoprotectants only, without disaccharides. This study aimed to search for and test a new method for in-vitro culture of vitrified tissue. Ovarian biop...
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| Veröffentlicht in: | Reproductive biomedicine online 2006, Vol.13 (2), p.228-234 |
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| creator | Isachenko, Vladimir Montag, Markus Isachenko, Evgenia van der Ven, Katrin Dorn, Christoph Roesing, Benjamin Braun, Feodor Sadek, Fatti van der Ven, Hans |
| description | It is widely accepted that it is possible to successfully cryopreserve human ovarian tissue by direct plunging into liquid nitrogen using permeable cryoprotectants only, without disaccharides. This study aimed to search for and test a new method for in-vitro culture of vitrified tissue. Ovarian biopsies were obtained during operative laparoscopy. Pieces of ovarian tissue were vitrified and warmed. After warming, tissue pieces were randomly distributed into three groups for further culture: in 2 ml of culture medium which was regularly renewed (group 1), in 30 ml of culture medium without agitation (group 2) and in 30 ml of culture medium with agitation (group 3). During the 2-week and 6-week culture, the growth of follicles within the vitrified-warmed ovarian tissue pieces was investigated. After 2 weeks of culture, mean numbers of non-degenerated follicles per mm
2 of tissue were 1.5, 1.7 and 4.5 for groups 1, 2 and 3 respectively (groups 1 and 2 versus group 3,
P < 0.05). Agitation during culture of ovarian tissue is beneficial, and can be used as a prognostic tool for future warming and autotransplantation of ovarian tissue. |
| doi_str_mv | 10.1016/S1472-6483(10)60620-7 |
| format | Article |
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2 of tissue were 1.5, 1.7 and 4.5 for groups 1, 2 and 3 respectively (groups 1 and 2 versus group 3,
P < 0.05). Agitation during culture of ovarian tissue is beneficial, and can be used as a prognostic tool for future warming and autotransplantation of ovarian tissue.</description><identifier>ISSN: 1472-6483</identifier><identifier>EISSN: 1472-6491</identifier><identifier>DOI: 10.1016/S1472-6483(10)60620-7</identifier><identifier>PMID: 16895638</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Cryopreservation ; culture ; Female ; follicles ; human ovarian tissue ; Humans ; Ovarian Follicle - cytology ; Ovarian Follicle - growth & development ; Ovary - cytology ; Tissue Culture Techniques - methods</subject><ispartof>Reproductive biomedicine online, 2006, Vol.13 (2), p.228-234</ispartof><rights>2006 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-300feda815dd618189bd1d834a54355d2d3f336a0ac50e4660e2ffd79bec93763</citedby><cites>FETCH-LOGICAL-c528t-300feda815dd618189bd1d834a54355d2d3f336a0ac50e4660e2ffd79bec93763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1472648310606207$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16895638$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Isachenko, Vladimir</creatorcontrib><creatorcontrib>Montag, Markus</creatorcontrib><creatorcontrib>Isachenko, Evgenia</creatorcontrib><creatorcontrib>van der Ven, Katrin</creatorcontrib><creatorcontrib>Dorn, Christoph</creatorcontrib><creatorcontrib>Roesing, Benjamin</creatorcontrib><creatorcontrib>Braun, Feodor</creatorcontrib><creatorcontrib>Sadek, Fatti</creatorcontrib><creatorcontrib>van der Ven, Hans</creatorcontrib><title>Effective method for in-vitro culture of cryopreserved human ovarian tissue</title><title>Reproductive biomedicine online</title><addtitle>Reprod Biomed Online</addtitle><description>It is widely accepted that it is possible to successfully cryopreserve human ovarian tissue by direct plunging into liquid nitrogen using permeable cryoprotectants only, without disaccharides. This study aimed to search for and test a new method for in-vitro culture of vitrified tissue. Ovarian biopsies were obtained during operative laparoscopy. Pieces of ovarian tissue were vitrified and warmed. After warming, tissue pieces were randomly distributed into three groups for further culture: in 2 ml of culture medium which was regularly renewed (group 1), in 30 ml of culture medium without agitation (group 2) and in 30 ml of culture medium with agitation (group 3). During the 2-week and 6-week culture, the growth of follicles within the vitrified-warmed ovarian tissue pieces was investigated. After 2 weeks of culture, mean numbers of non-degenerated follicles per mm
2 of tissue were 1.5, 1.7 and 4.5 for groups 1, 2 and 3 respectively (groups 1 and 2 versus group 3,
P < 0.05). Agitation during culture of ovarian tissue is beneficial, and can be used as a prognostic tool for future warming and autotransplantation of ovarian tissue.</description><subject>Cryopreservation</subject><subject>culture</subject><subject>Female</subject><subject>follicles</subject><subject>human ovarian tissue</subject><subject>Humans</subject><subject>Ovarian Follicle - cytology</subject><subject>Ovarian Follicle - growth & development</subject><subject>Ovary - cytology</subject><subject>Tissue Culture Techniques - methods</subject><issn>1472-6483</issn><issn>1472-6491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1OwzAQhC0EolB4BFBOCA4BO44d54RQVX5EJQ7A2XLttWqUxMVOIvXtSdsIjpx2NZrZ0X4IXRB8SzDhd-8kL7KU54JeE3zDMc9wWhygk1EuyeHvLugEncb4hTERWNBjNCFclIxTcYJe59aCbl0PSQ3typvE-pC4Ju1dG3yiu6rtAiTeJjps_DpAhNCDSVZdrZrE9yq4YbYuxg7O0JFVVYTzcU7R5-P8Y_acLt6eXmYPi1SzTLQpxdiCUYIwYzgRRJRLQ4yguWI5ZcxkhlpKucJKMww55xgya01RLkGXtOB0iq72d9fBf3cQW1m7qKGqVAO-i5KLImOUl4OR7Y06-BgDWLkOrlZhIwmWW4pyR1FuEW2lHUVZDLnLsaBb1mD-UiO2wXC_N8DwZu8gyKgdNBqMCwNNabz7p-IHbAGCRw</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Isachenko, Vladimir</creator><creator>Montag, Markus</creator><creator>Isachenko, Evgenia</creator><creator>van der Ven, Katrin</creator><creator>Dorn, Christoph</creator><creator>Roesing, Benjamin</creator><creator>Braun, Feodor</creator><creator>Sadek, Fatti</creator><creator>van der Ven, Hans</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2006</creationdate><title>Effective method for in-vitro culture of cryopreserved human ovarian tissue</title><author>Isachenko, Vladimir ; Montag, Markus ; Isachenko, Evgenia ; van der Ven, Katrin ; Dorn, Christoph ; Roesing, Benjamin ; Braun, Feodor ; Sadek, Fatti ; van der Ven, Hans</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-300feda815dd618189bd1d834a54355d2d3f336a0ac50e4660e2ffd79bec93763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Cryopreservation</topic><topic>culture</topic><topic>Female</topic><topic>follicles</topic><topic>human ovarian tissue</topic><topic>Humans</topic><topic>Ovarian Follicle - cytology</topic><topic>Ovarian Follicle - growth & development</topic><topic>Ovary - cytology</topic><topic>Tissue Culture Techniques - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Isachenko, Vladimir</creatorcontrib><creatorcontrib>Montag, Markus</creatorcontrib><creatorcontrib>Isachenko, Evgenia</creatorcontrib><creatorcontrib>van der Ven, Katrin</creatorcontrib><creatorcontrib>Dorn, Christoph</creatorcontrib><creatorcontrib>Roesing, Benjamin</creatorcontrib><creatorcontrib>Braun, Feodor</creatorcontrib><creatorcontrib>Sadek, Fatti</creatorcontrib><creatorcontrib>van der Ven, Hans</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive biomedicine online</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Isachenko, Vladimir</au><au>Montag, Markus</au><au>Isachenko, Evgenia</au><au>van der Ven, Katrin</au><au>Dorn, Christoph</au><au>Roesing, Benjamin</au><au>Braun, Feodor</au><au>Sadek, Fatti</au><au>van der Ven, Hans</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effective method for in-vitro culture of cryopreserved human ovarian tissue</atitle><jtitle>Reproductive biomedicine online</jtitle><addtitle>Reprod Biomed Online</addtitle><date>2006</date><risdate>2006</risdate><volume>13</volume><issue>2</issue><spage>228</spage><epage>234</epage><pages>228-234</pages><issn>1472-6483</issn><eissn>1472-6491</eissn><abstract>It is widely accepted that it is possible to successfully cryopreserve human ovarian tissue by direct plunging into liquid nitrogen using permeable cryoprotectants only, without disaccharides. This study aimed to search for and test a new method for in-vitro culture of vitrified tissue. Ovarian biopsies were obtained during operative laparoscopy. Pieces of ovarian tissue were vitrified and warmed. After warming, tissue pieces were randomly distributed into three groups for further culture: in 2 ml of culture medium which was regularly renewed (group 1), in 30 ml of culture medium without agitation (group 2) and in 30 ml of culture medium with agitation (group 3). During the 2-week and 6-week culture, the growth of follicles within the vitrified-warmed ovarian tissue pieces was investigated. After 2 weeks of culture, mean numbers of non-degenerated follicles per mm
2 of tissue were 1.5, 1.7 and 4.5 for groups 1, 2 and 3 respectively (groups 1 and 2 versus group 3,
P < 0.05). Agitation during culture of ovarian tissue is beneficial, and can be used as a prognostic tool for future warming and autotransplantation of ovarian tissue.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>16895638</pmid><doi>10.1016/S1472-6483(10)60620-7</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Cryopreservation culture Female follicles human ovarian tissue Humans Ovarian Follicle - cytology Ovarian Follicle - growth & development Ovary - cytology Tissue Culture Techniques - methods |
| title | Effective method for in-vitro culture of cryopreserved human ovarian tissue |
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