Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon
Amoebic gill disease (AGD), the most serious infectious disease affecting farmed salmon in Tasmania, is caused by free-living marine amoeba Neoparamoeba sp. The parasites on the gills induce proliferation of epithelial cells initiating a hyperplastic response and reducing the surface area available...
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| Veröffentlicht in: | International journal for parasitology 2005-11, Vol.35 (13), p.1417-1423 |
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| creator | Villavedra, Margarita McCarthy, Kristy To, Joyce Morrison, Richard Crosbie, Philip Broady, Kevin Raison, Robert L. |
| description | Amoebic gill disease (AGD), the most serious infectious disease affecting farmed salmon in Tasmania, is caused by free-living marine amoeba
Neoparamoeba sp. The parasites on the gills induce proliferation of epithelial cells initiating a hyperplastic response and reducing the surface area available for gaseous exchange. AGD can be induced in salmon by exposure to freshly isolated
Neoparamoeba from AGD infected fish, however cultured
Neoparamoeba are non-infective. We describe here antigenic differences between freshly isolated and in vitro cultured parasites, and within individual isolates of the parasite cultured under different conditions. Immunoblot analysis using polyclonal antisera, revealed differences in the antigen profiles of two cultured isolates of
Neoparamoeba sp. when they were grown on agar versus in liquid medium. However, the antigen profiles of the two isolates were very similar when they were grown under the same culture conditions. Comparison of these antigen profiles with a preparation from parasites freshly isolated from infected gills revealed a very limited number of shared antigens. In addition monoclonal antibodies (mAbs) raised against surface antigens of cultured parasites were used in an indirect immunofluorescence assay to assess the expression of specific surface antigens of
Neoparamoeba sp. after various periods in culture. Significant changes in antigen expression of freshly isolated parasites were observed after 15 days of in vitro culture. The use of mAb demonstrated progressive exposure/expression of individual antigens on the surface of the freshly isolated parasites during the period in culture. |
| doi_str_mv | 10.1016/j.ijpara.2005.05.014 |
| format | Article |
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Neoparamoeba sp. The parasites on the gills induce proliferation of epithelial cells initiating a hyperplastic response and reducing the surface area available for gaseous exchange. AGD can be induced in salmon by exposure to freshly isolated
Neoparamoeba from AGD infected fish, however cultured
Neoparamoeba are non-infective. We describe here antigenic differences between freshly isolated and in vitro cultured parasites, and within individual isolates of the parasite cultured under different conditions. Immunoblot analysis using polyclonal antisera, revealed differences in the antigen profiles of two cultured isolates of
Neoparamoeba sp. when they were grown on agar versus in liquid medium. However, the antigen profiles of the two isolates were very similar when they were grown under the same culture conditions. Comparison of these antigen profiles with a preparation from parasites freshly isolated from infected gills revealed a very limited number of shared antigens. In addition monoclonal antibodies (mAbs) raised against surface antigens of cultured parasites were used in an indirect immunofluorescence assay to assess the expression of specific surface antigens of
Neoparamoeba sp. after various periods in culture. Significant changes in antigen expression of freshly isolated parasites were observed after 15 days of in vitro culture. The use of mAb demonstrated progressive exposure/expression of individual antigens on the surface of the freshly isolated parasites during the period in culture.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/j.ijpara.2005.05.014</identifier><identifier>PMID: 16102771</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Agnatha. Pisces ; amebiasis ; Amebiasis - immunology ; Amebiasis - veterinary ; Amoeba ; Amoeba - immunology ; Amoebic gill disease ; Amoebida ; Animals ; Antibodies, Monoclonal - immunology ; Antigen ; antigens ; Antigens, Protozoan - analysis ; Antigens, Protozoan - immunology ; Atlantic salmon ; Biological and medical sciences ; cell culture ; Culture ; culture media ; cultured cells ; farmed fish ; fish diseases ; Fish Diseases - immunology ; Fish Diseases - parasitology ; Fisheries ; Fluorescent Antibody Technique, Indirect - methods ; Fundamental and applied biological sciences. Psychology ; Gills - parasitology ; In Vitro Techniques ; Life cycle. Host-agent relationship. Pathogenesis ; Marine ; Mice ; Mice, Inbred BALB C ; monoclonal antibodies ; Neoparamoeba ; Protozoa ; Salmo salar ; Salmo salar - immunology ; Salmo salar - parasitology ; surface antigens ; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><ispartof>International journal for parasitology, 2005-11, Vol.35 (13), p.1417-1423</ispartof><rights>2005 Australian Society for Parasitology Inc</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-f89ddea8c4ec82dd6892e302445d9a827d046ee1fe7a223bcdfc7f60f695c503</citedby><cites>FETCH-LOGICAL-c445t-f89ddea8c4ec82dd6892e302445d9a827d046ee1fe7a223bcdfc7f60f695c503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijpara.2005.05.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17230631$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16102771$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Villavedra, Margarita</creatorcontrib><creatorcontrib>McCarthy, Kristy</creatorcontrib><creatorcontrib>To, Joyce</creatorcontrib><creatorcontrib>Morrison, Richard</creatorcontrib><creatorcontrib>Crosbie, Philip</creatorcontrib><creatorcontrib>Broady, Kevin</creatorcontrib><creatorcontrib>Raison, Robert L.</creatorcontrib><title>Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>Amoebic gill disease (AGD), the most serious infectious disease affecting farmed salmon in Tasmania, is caused by free-living marine amoeba
Neoparamoeba sp. The parasites on the gills induce proliferation of epithelial cells initiating a hyperplastic response and reducing the surface area available for gaseous exchange. AGD can be induced in salmon by exposure to freshly isolated
Neoparamoeba from AGD infected fish, however cultured
Neoparamoeba are non-infective. We describe here antigenic differences between freshly isolated and in vitro cultured parasites, and within individual isolates of the parasite cultured under different conditions. Immunoblot analysis using polyclonal antisera, revealed differences in the antigen profiles of two cultured isolates of
Neoparamoeba sp. when they were grown on agar versus in liquid medium. However, the antigen profiles of the two isolates were very similar when they were grown under the same culture conditions. Comparison of these antigen profiles with a preparation from parasites freshly isolated from infected gills revealed a very limited number of shared antigens. In addition monoclonal antibodies (mAbs) raised against surface antigens of cultured parasites were used in an indirect immunofluorescence assay to assess the expression of specific surface antigens of
Neoparamoeba sp. after various periods in culture. Significant changes in antigen expression of freshly isolated parasites were observed after 15 days of in vitro culture. The use of mAb demonstrated progressive exposure/expression of individual antigens on the surface of the freshly isolated parasites during the period in culture.</description><subject>Agnatha. Pisces</subject><subject>amebiasis</subject><subject>Amebiasis - immunology</subject><subject>Amebiasis - veterinary</subject><subject>Amoeba</subject><subject>Amoeba - immunology</subject><subject>Amoebic gill disease</subject><subject>Amoebida</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigen</subject><subject>antigens</subject><subject>Antigens, Protozoan - analysis</subject><subject>Antigens, Protozoan - immunology</subject><subject>Atlantic salmon</subject><subject>Biological and medical sciences</subject><subject>cell culture</subject><subject>Culture</subject><subject>culture media</subject><subject>cultured cells</subject><subject>farmed fish</subject><subject>fish diseases</subject><subject>Fish Diseases - immunology</subject><subject>Fish Diseases - parasitology</subject><subject>Fisheries</subject><subject>Fluorescent Antibody Technique, Indirect - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gills - parasitology</subject><subject>In Vitro Techniques</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Marine</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>monoclonal antibodies</subject><subject>Neoparamoeba</subject><subject>Protozoa</subject><subject>Salmo salar</subject><subject>Salmo salar - immunology</subject><subject>Salmo salar - parasitology</subject><subject>surface antigens</subject><subject>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7uzqPxDNxT3ZbSWdpLsvwjL4BYseXM8hk1TaDP0xJt0L4p83bQ_sTaEgh3rqfavyEvKCQcmAqbfHMhxPJpqSA8hyLSYekR1r6rYAVsnHZAfAoaglay_IZUpHACYrIZ6SC6YY8LpmO_J7_8OMHSYaRmrGOXQ4BktPcfKhR-qWGMaO2qWfl4h08vQLTqvpMOHB0HQq31BrlmTmcI_U5OF5hf62s0wX-p66kNAkXA1u5n71sDSZfpjGZ-SJN33C5-f3itx9eH-3_1Tcfv34eX9zW1gh5Fz4pnUOTWMF2oY7p5qWYwU8N11rGl47EAqReawN59XBOm9rr8CrVloJ1RW53mTzVT8XTLMeQrLY511wWpJWTc05NPy_IGslV1LVGRQbaOOUUkSvTzEMJv7SDPQajj7qLRy9hqPXYiKPvTzrL4cB3cPQOY0MvD4DJlnT-2hGG9IDV_MKVLVyrzbOm0mbLmbm-zeeQwcGommlzMS7jcD8r_cBo0424GjRhYh21m4K_971D77BuZI</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Villavedra, Margarita</creator><creator>McCarthy, Kristy</creator><creator>To, Joyce</creator><creator>Morrison, Richard</creator><creator>Crosbie, Philip</creator><creator>Broady, Kevin</creator><creator>Raison, Robert L.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20051101</creationdate><title>Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon</title><author>Villavedra, Margarita ; McCarthy, Kristy ; To, Joyce ; Morrison, Richard ; Crosbie, Philip ; Broady, Kevin ; Raison, Robert L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-f89ddea8c4ec82dd6892e302445d9a827d046ee1fe7a223bcdfc7f60f695c503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Agnatha. 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Psychology</topic><topic>Gills - parasitology</topic><topic>In Vitro Techniques</topic><topic>Life cycle. Host-agent relationship. 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Neoparamoeba sp. The parasites on the gills induce proliferation of epithelial cells initiating a hyperplastic response and reducing the surface area available for gaseous exchange. AGD can be induced in salmon by exposure to freshly isolated
Neoparamoeba from AGD infected fish, however cultured
Neoparamoeba are non-infective. We describe here antigenic differences between freshly isolated and in vitro cultured parasites, and within individual isolates of the parasite cultured under different conditions. Immunoblot analysis using polyclonal antisera, revealed differences in the antigen profiles of two cultured isolates of
Neoparamoeba sp. when they were grown on agar versus in liquid medium. However, the antigen profiles of the two isolates were very similar when they were grown under the same culture conditions. Comparison of these antigen profiles with a preparation from parasites freshly isolated from infected gills revealed a very limited number of shared antigens. In addition monoclonal antibodies (mAbs) raised against surface antigens of cultured parasites were used in an indirect immunofluorescence assay to assess the expression of specific surface antigens of
Neoparamoeba sp. after various periods in culture. Significant changes in antigen expression of freshly isolated parasites were observed after 15 days of in vitro culture. The use of mAb demonstrated progressive exposure/expression of individual antigens on the surface of the freshly isolated parasites during the period in culture.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16102771</pmid><doi>10.1016/j.ijpara.2005.05.014</doi><tpages>7</tpages></addata></record> |
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| ispartof | International journal for parasitology, 2005-11, Vol.35 (13), p.1417-1423 |
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| subjects | Agnatha. Pisces amebiasis Amebiasis - immunology Amebiasis - veterinary Amoeba Amoeba - immunology Amoebic gill disease Amoebida Animals Antibodies, Monoclonal - immunology Antigen antigens Antigens, Protozoan - analysis Antigens, Protozoan - immunology Atlantic salmon Biological and medical sciences cell culture Culture culture media cultured cells farmed fish fish diseases Fish Diseases - immunology Fish Diseases - parasitology Fisheries Fluorescent Antibody Technique, Indirect - methods Fundamental and applied biological sciences. Psychology Gills - parasitology In Vitro Techniques Life cycle. Host-agent relationship. Pathogenesis Marine Mice Mice, Inbred BALB C monoclonal antibodies Neoparamoeba Protozoa Salmo salar Salmo salar - immunology Salmo salar - parasitology surface antigens Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution |
| title | Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon |
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