Multiple Isotope Effect Study of the Hydrolysis of Formamide by Urease from Jack Bean (Canavalia ensiformis)

Multiple kinetic isotope effects have been measured for the urease-catalyzed hydrolysis of formamide at pH 6.0 and 25 °C. These kinetic isotope effects include the carbonyl-C (13 k = 1.0241 ± 0.0009), the carbonyl-O (18 k = 0.9960 ± 0.0009), the formyl-H (D k = 0.95 ± 0.01), the leaving-N (15 k = 1....

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Veröffentlicht in:	Biochemistry (Easton) 2006-08, Vol.45 (32), p.9940-9948
Hauptverfasser:	Marlier, John F, Cleland, W. W
Format:	Artikel
Sprache:	eng
Schlagworte:	Canavalia - enzymology Cysteine - metabolism Formamides - metabolism Formates - metabolism Hydrogen-Ion Concentration Hydrolysis Oxygen - metabolism Oxygen Isotopes - metabolism Urea - metabolism Urease - metabolism
Online-Zugang:	Volltext
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