A Posttranscriptional Role for the Yeast Paf1-RNA Polymerase II Complex Is Revealed by Identification of Primary Targets
The yeast Paf1 complex (Paf1C: Paf1, Cdc73, Ctr9, Rtf1, and Leo1) is associated with RNA Polymerase II (Pol II) at promoters and coding regions of transcriptionally active genes, but transcript abundance for only a small subset of genes is altered by loss of Paf1. By using conditional and null allel...
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| Veröffentlicht in: | Molecular cell 2005-10, Vol.20 (2), p.213-223 |
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| creator | Penheiter, Kristi L. Washburn, Taylor M. Porter, Stephanie E. Hoffman, Matthew G. Jaehning, Judith A. |
| description | The yeast Paf1 complex (Paf1C: Paf1, Cdc73, Ctr9, Rtf1, and Leo1) is associated with RNA Polymerase II (Pol II) at promoters and coding regions of transcriptionally active genes, but transcript abundance for only a small subset of genes is altered by loss of Paf1. By using conditional and null alleles of
PAF1 and microarrays, we determined the identity of both primary and secondary targets of the Paf1C. Neither primary nor secondary Paf1C target promoters were responsive to loss of Paf1. Instead, Paf1 loss altered poly(A) site utilization of primary target genes
SDA1 and
MAK21, resulting in increased abundance of 3′-extended mRNAs. The 3′-extended
MAK21 RNA is sensitive to nonsense-mediated decay (NMD), as revealed by its increased abundance in the absence of Upf1. Therefore, although the Paf1C is associated with Pol II at initiation and during elongation, these critical Paf1-dependent changes in transcript abundance are due to alterations in posttranscriptional processing. |
| doi_str_mv | 10.1016/j.molcel.2005.08.023 |
| format | Article |
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PAF1 and microarrays, we determined the identity of both primary and secondary targets of the Paf1C. Neither primary nor secondary Paf1C target promoters were responsive to loss of Paf1. Instead, Paf1 loss altered poly(A) site utilization of primary target genes
SDA1 and
MAK21, resulting in increased abundance of 3′-extended mRNAs. The 3′-extended
MAK21 RNA is sensitive to nonsense-mediated decay (NMD), as revealed by its increased abundance in the absence of Upf1. Therefore, although the Paf1C is associated with Pol II at initiation and during elongation, these critical Paf1-dependent changes in transcript abundance are due to alterations in posttranscriptional processing.</description><identifier>ISSN: 1097-2765</identifier><identifier>EISSN: 1097-4164</identifier><identifier>DOI: 10.1016/j.molcel.2005.08.023</identifier><identifier>PMID: 16246724</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Gene Expression Regulation ; Macromolecular Substances - metabolism ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; RNA Polymerase II - metabolism ; RNA Processing, Post-Transcriptional - physiology ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Transcription, Genetic</subject><ispartof>Molecular cell, 2005-10, Vol.20 (2), p.213-223</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-668801394bc577f7ae1bed250e4a1581beaa848ebe3e48e44f5931156c967c5e3</citedby><cites>FETCH-LOGICAL-c472t-668801394bc577f7ae1bed250e4a1581beaa848ebe3e48e44f5931156c967c5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.molcel.2005.08.023$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16246724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Penheiter, Kristi L.</creatorcontrib><creatorcontrib>Washburn, Taylor M.</creatorcontrib><creatorcontrib>Porter, Stephanie E.</creatorcontrib><creatorcontrib>Hoffman, Matthew G.</creatorcontrib><creatorcontrib>Jaehning, Judith A.</creatorcontrib><title>A Posttranscriptional Role for the Yeast Paf1-RNA Polymerase II Complex Is Revealed by Identification of Primary Targets</title><title>Molecular cell</title><addtitle>Mol Cell</addtitle><description>The yeast Paf1 complex (Paf1C: Paf1, Cdc73, Ctr9, Rtf1, and Leo1) is associated with RNA Polymerase II (Pol II) at promoters and coding regions of transcriptionally active genes, but transcript abundance for only a small subset of genes is altered by loss of Paf1. By using conditional and null alleles of
PAF1 and microarrays, we determined the identity of both primary and secondary targets of the Paf1C. Neither primary nor secondary Paf1C target promoters were responsive to loss of Paf1. Instead, Paf1 loss altered poly(A) site utilization of primary target genes
SDA1 and
MAK21, resulting in increased abundance of 3′-extended mRNAs. The 3′-extended
MAK21 RNA is sensitive to nonsense-mediated decay (NMD), as revealed by its increased abundance in the absence of Upf1. Therefore, although the Paf1C is associated with Pol II at initiation and during elongation, these critical Paf1-dependent changes in transcript abundance are due to alterations in posttranscriptional processing.</description><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Macromolecular Substances - metabolism</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>RNA Polymerase II - metabolism</subject><subject>RNA Processing, Post-Transcriptional - physiology</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Transcription, Genetic</subject><issn>1097-2765</issn><issn>1097-4164</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2L2zAQhkVp6X60_6AUnXqzK8n68qWwhH4YljaE7aEnIcvjVkG2UklZNv--Dg70tqfRwDPvjB6E3lFSU0Llx309xeAg1IwQURNdE9a8QNeUtKriVPKXlzdTUlyhm5z3hFAudPsaXVHJuFSMX6OnO7yNuZRk5-ySPxQfZxvwLgbAY0y4_AH8C2wueGtHWu2-n_lwmiDZDLjr8CZOhwBPuMt4B49gAwy4P-FugLn40Tt7TsRxxNvkJ5tO-MGm31DyG_RqtCHD20u9RT-_fH7YfKvuf3ztNnf3leOKlUpKrQltWt47odSoLNAeBiYIcEuFXhprNdfQQwNL4XwUbUOpkK6VyglobtGHNfeQ4t8j5GImnxdtwc4Qj9lIrRjlTCwgX0GXYs4JRnNYLzaUmLNxszercXM2bog2i_Fl7P0l_9hPMPwfuihegE8rAMsvHz0kk52H2cHgE7hihuif3_AP04OUHw</recordid><startdate>20051028</startdate><enddate>20051028</enddate><creator>Penheiter, Kristi L.</creator><creator>Washburn, Taylor M.</creator><creator>Porter, Stephanie E.</creator><creator>Hoffman, Matthew G.</creator><creator>Jaehning, Judith A.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051028</creationdate><title>A Posttranscriptional Role for the Yeast Paf1-RNA Polymerase II Complex Is Revealed by Identification of Primary Targets</title><author>Penheiter, Kristi L. ; Washburn, Taylor M. ; Porter, Stephanie E. ; Hoffman, Matthew G. ; Jaehning, Judith A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-668801394bc577f7ae1bed250e4a1581beaa848ebe3e48e44f5931156c967c5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Macromolecular Substances - metabolism</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>RNA Polymerase II - metabolism</topic><topic>RNA Processing, Post-Transcriptional - physiology</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Penheiter, Kristi L.</creatorcontrib><creatorcontrib>Washburn, Taylor M.</creatorcontrib><creatorcontrib>Porter, Stephanie E.</creatorcontrib><creatorcontrib>Hoffman, Matthew G.</creatorcontrib><creatorcontrib>Jaehning, Judith A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Penheiter, Kristi L.</au><au>Washburn, Taylor M.</au><au>Porter, Stephanie E.</au><au>Hoffman, Matthew G.</au><au>Jaehning, Judith A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Posttranscriptional Role for the Yeast Paf1-RNA Polymerase II Complex Is Revealed by Identification of Primary Targets</atitle><jtitle>Molecular cell</jtitle><addtitle>Mol Cell</addtitle><date>2005-10-28</date><risdate>2005</risdate><volume>20</volume><issue>2</issue><spage>213</spage><epage>223</epage><pages>213-223</pages><issn>1097-2765</issn><eissn>1097-4164</eissn><abstract>The yeast Paf1 complex (Paf1C: Paf1, Cdc73, Ctr9, Rtf1, and Leo1) is associated with RNA Polymerase II (Pol II) at promoters and coding regions of transcriptionally active genes, but transcript abundance for only a small subset of genes is altered by loss of Paf1. By using conditional and null alleles of
PAF1 and microarrays, we determined the identity of both primary and secondary targets of the Paf1C. Neither primary nor secondary Paf1C target promoters were responsive to loss of Paf1. Instead, Paf1 loss altered poly(A) site utilization of primary target genes
SDA1 and
MAK21, resulting in increased abundance of 3′-extended mRNAs. The 3′-extended
MAK21 RNA is sensitive to nonsense-mediated decay (NMD), as revealed by its increased abundance in the absence of Upf1. Therefore, although the Paf1C is associated with Pol II at initiation and during elongation, these critical Paf1-dependent changes in transcript abundance are due to alterations in posttranscriptional processing.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16246724</pmid><doi>10.1016/j.molcel.2005.08.023</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Gene Expression Regulation Macromolecular Substances - metabolism Nuclear Proteins - genetics Nuclear Proteins - metabolism RNA Polymerase II - metabolism RNA Processing, Post-Transcriptional - physiology RNA, Messenger - genetics RNA, Messenger - metabolism Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Transcription, Genetic |
| title | A Posttranscriptional Role for the Yeast Paf1-RNA Polymerase II Complex Is Revealed by Identification of Primary Targets |
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