Recombinant antigens as diagnostic markers for aspergillosis
Eight recombinant proteins and purified galactomannan of Aspergillus fumigatus were tested by enzyme-linked immunosorbent assay to quantify the anti- Aspergillus antibodies in sera of patients with aspergilloma, allergic bronchopulmonary aspergillosis (ABPA), and invasive aspergillosis (IA). In spit...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2006-08, Vol.55 (4), p.279-291 |
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| creator | Sarfati, Jacqueline Monod, Michael Recco, Paulette Sulahian, Annie Pinel, Claudine Candolfi, Ermanno Fontaine, Thierry Debeaupuis, Jean-Paul Tabouret, Marc Latgé, Jean-Paul |
| description | Eight recombinant proteins and purified galactomannan of
Aspergillus fumigatus were tested by enzyme-linked immunosorbent assay to quantify the anti-
Aspergillus antibodies in sera of patients with aspergilloma, allergic bronchopulmonary aspergillosis (ABPA), and invasive aspergillosis (IA). In spite of the variability observed in the immune responses of individual patients, quantification of the antibody titers against the 18-kDa ribonuclease (RNU), the 360-kDa catalase (CAT), and the 88-kDa dipeptidylpeptidase V (DPPV) was useful for the diagnosis of aspergilloma and ABPA. Differential diagnosis of ABPA was even possible among cystic fibrosis as well as noncystic fibrosis patients. In the group of immunocompromised patients with IA, no antibody response was mounted in response to the
Aspergillus infection in any of the patients. Interestingly, about half of the patients with proven IA came to the hospital with high titers of anti-
Aspergillus antibodies, suggesting that they were infected upon entry to the hospital. These results suggest that recombinant RNU, CAT, and DPPV have a great potential in the serodiagnosis of all forms of aspergillosis in the immunocompromised and immunocompetent patient. |
| doi_str_mv | 10.1016/j.diagmicrobio.2006.02.002 |
| format | Article |
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Aspergillus fumigatus were tested by enzyme-linked immunosorbent assay to quantify the anti-
Aspergillus antibodies in sera of patients with aspergilloma, allergic bronchopulmonary aspergillosis (ABPA), and invasive aspergillosis (IA). In spite of the variability observed in the immune responses of individual patients, quantification of the antibody titers against the 18-kDa ribonuclease (RNU), the 360-kDa catalase (CAT), and the 88-kDa dipeptidylpeptidase V (DPPV) was useful for the diagnosis of aspergilloma and ABPA. Differential diagnosis of ABPA was even possible among cystic fibrosis as well as noncystic fibrosis patients. In the group of immunocompromised patients with IA, no antibody response was mounted in response to the
Aspergillus infection in any of the patients. Interestingly, about half of the patients with proven IA came to the hospital with high titers of anti-
Aspergillus antibodies, suggesting that they were infected upon entry to the hospital. These results suggest that recombinant RNU, CAT, and DPPV have a great potential in the serodiagnosis of all forms of aspergillosis in the immunocompromised and immunocompetent patient.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2006.02.002</identifier><identifier>PMID: 16626916</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Antigens ; Antigens, Bacterial - biosynthesis ; Antigens, Bacterial - immunology ; Aspergillosis ; Aspergillosis - diagnosis ; Aspergillosis - immunology ; Aspergillus fumigatus ; Aspergillus fumigatus - genetics ; Aspergillus fumigatus - immunology ; Biological and medical sciences ; Catalase ; Cystic Fibrosis - immunology ; Cystic Fibrosis - microbiology ; Dipeptidylpeptidase ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Fundamental and applied biological sciences. Psychology ; Genetic Vectors - biosynthesis ; Humans ; Infectious diseases ; Medical sciences ; Microbiology ; Miscellaneous ; Mycology ; Pichia ; Predictive Value of Tests ; Recombinant Proteins - immunology ; Ribonuclease</subject><ispartof>Diagnostic microbiology and infectious disease, 2006-08, Vol.55 (4), p.279-291</ispartof><rights>2006 Elsevier Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-2120ba7ff61d9b3c7cc9b133add59c125cdbe54cc3400b5b489f1d6e6860cbca3</citedby><cites>FETCH-LOGICAL-c439t-2120ba7ff61d9b3c7cc9b133add59c125cdbe54cc3400b5b489f1d6e6860cbca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0732889306000587$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18031784$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16626916$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sarfati, Jacqueline</creatorcontrib><creatorcontrib>Monod, Michael</creatorcontrib><creatorcontrib>Recco, Paulette</creatorcontrib><creatorcontrib>Sulahian, Annie</creatorcontrib><creatorcontrib>Pinel, Claudine</creatorcontrib><creatorcontrib>Candolfi, Ermanno</creatorcontrib><creatorcontrib>Fontaine, Thierry</creatorcontrib><creatorcontrib>Debeaupuis, Jean-Paul</creatorcontrib><creatorcontrib>Tabouret, Marc</creatorcontrib><creatorcontrib>Latgé, Jean-Paul</creatorcontrib><title>Recombinant antigens as diagnostic markers for aspergillosis</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Eight recombinant proteins and purified galactomannan of
Aspergillus fumigatus were tested by enzyme-linked immunosorbent assay to quantify the anti-
Aspergillus antibodies in sera of patients with aspergilloma, allergic bronchopulmonary aspergillosis (ABPA), and invasive aspergillosis (IA). In spite of the variability observed in the immune responses of individual patients, quantification of the antibody titers against the 18-kDa ribonuclease (RNU), the 360-kDa catalase (CAT), and the 88-kDa dipeptidylpeptidase V (DPPV) was useful for the diagnosis of aspergilloma and ABPA. Differential diagnosis of ABPA was even possible among cystic fibrosis as well as noncystic fibrosis patients. In the group of immunocompromised patients with IA, no antibody response was mounted in response to the
Aspergillus infection in any of the patients. Interestingly, about half of the patients with proven IA came to the hospital with high titers of anti-
Aspergillus antibodies, suggesting that they were infected upon entry to the hospital. These results suggest that recombinant RNU, CAT, and DPPV have a great potential in the serodiagnosis of all forms of aspergillosis in the immunocompromised and immunocompetent patient.</description><subject>Antigens</subject><subject>Antigens, Bacterial - biosynthesis</subject><subject>Antigens, Bacterial - immunology</subject><subject>Aspergillosis</subject><subject>Aspergillosis - diagnosis</subject><subject>Aspergillosis - immunology</subject><subject>Aspergillus fumigatus</subject><subject>Aspergillus fumigatus - genetics</subject><subject>Aspergillus fumigatus - immunology</subject><subject>Biological and medical sciences</subject><subject>Catalase</subject><subject>Cystic Fibrosis - immunology</subject><subject>Cystic Fibrosis - microbiology</subject><subject>Dipeptidylpeptidase</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Vectors - biosynthesis</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Pichia</subject><subject>Predictive Value of Tests</subject><subject>Recombinant Proteins - immunology</subject><subject>Ribonuclease</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtr3DAQgEVpaLZp_0IxgfRmZyTZslRyCUn6gEAgtGchjeRFW9vaSN5A_3207EJ6aw_DHOab10fIOYWGAhWXm8YFs54CpmhDbBiAaIA1AOwNWVHZqxqgh7dkBT1ntZSKn5L3OW8AKFMtvCOnVAgmFBUrcvXoMU42zGZeqhJh7edcmVztV8wxLwGryaTfPuVqiKlUtj6twzjGHPIHcjKYMfuPx3xGfn29-3nzvb5_-Pbj5vq-xparpWaUgTX9MAjqlOXYIypLOTfOdQop69BZ37WIvAWwnW2lGqgTXkgBaNHwM_L5MHeb4tPO50VPIaMfRzP7uMtayJ4yweQ_QdqztrzeFfDLASwOc05-0NsUyp9_NAW9l6w3-m_Jei9ZA9NFcmn-dNyys5N3r61HqwW4OAImoxmHZGYM-ZWTwGkv28LdHjhf5D0Hn3TG4Gf0LiSPi3Yx_M89L9Tzoio</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Sarfati, Jacqueline</creator><creator>Monod, Michael</creator><creator>Recco, Paulette</creator><creator>Sulahian, Annie</creator><creator>Pinel, Claudine</creator><creator>Candolfi, Ermanno</creator><creator>Fontaine, Thierry</creator><creator>Debeaupuis, Jean-Paul</creator><creator>Tabouret, Marc</creator><creator>Latgé, Jean-Paul</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>Recombinant antigens as diagnostic markers for aspergillosis</title><author>Sarfati, Jacqueline ; Monod, Michael ; Recco, Paulette ; Sulahian, Annie ; Pinel, Claudine ; Candolfi, Ermanno ; Fontaine, Thierry ; Debeaupuis, Jean-Paul ; Tabouret, Marc ; Latgé, Jean-Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-2120ba7ff61d9b3c7cc9b133add59c125cdbe54cc3400b5b489f1d6e6860cbca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Antigens</topic><topic>Antigens, Bacterial - biosynthesis</topic><topic>Antigens, Bacterial - immunology</topic><topic>Aspergillosis</topic><topic>Aspergillosis - diagnosis</topic><topic>Aspergillosis - immunology</topic><topic>Aspergillus fumigatus</topic><topic>Aspergillus fumigatus - genetics</topic><topic>Aspergillus fumigatus - immunology</topic><topic>Biological and medical sciences</topic><topic>Catalase</topic><topic>Cystic Fibrosis - immunology</topic><topic>Cystic Fibrosis - microbiology</topic><topic>Dipeptidylpeptidase</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Vectors - biosynthesis</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Pichia</topic><topic>Predictive Value of Tests</topic><topic>Recombinant Proteins - immunology</topic><topic>Ribonuclease</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarfati, Jacqueline</creatorcontrib><creatorcontrib>Monod, Michael</creatorcontrib><creatorcontrib>Recco, Paulette</creatorcontrib><creatorcontrib>Sulahian, Annie</creatorcontrib><creatorcontrib>Pinel, Claudine</creatorcontrib><creatorcontrib>Candolfi, Ermanno</creatorcontrib><creatorcontrib>Fontaine, Thierry</creatorcontrib><creatorcontrib>Debeaupuis, Jean-Paul</creatorcontrib><creatorcontrib>Tabouret, Marc</creatorcontrib><creatorcontrib>Latgé, Jean-Paul</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarfati, Jacqueline</au><au>Monod, Michael</au><au>Recco, Paulette</au><au>Sulahian, Annie</au><au>Pinel, Claudine</au><au>Candolfi, Ermanno</au><au>Fontaine, Thierry</au><au>Debeaupuis, Jean-Paul</au><au>Tabouret, Marc</au><au>Latgé, Jean-Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant antigens as diagnostic markers for aspergillosis</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>55</volume><issue>4</issue><spage>279</spage><epage>291</epage><pages>279-291</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Eight recombinant proteins and purified galactomannan of
Aspergillus fumigatus were tested by enzyme-linked immunosorbent assay to quantify the anti-
Aspergillus antibodies in sera of patients with aspergilloma, allergic bronchopulmonary aspergillosis (ABPA), and invasive aspergillosis (IA). In spite of the variability observed in the immune responses of individual patients, quantification of the antibody titers against the 18-kDa ribonuclease (RNU), the 360-kDa catalase (CAT), and the 88-kDa dipeptidylpeptidase V (DPPV) was useful for the diagnosis of aspergilloma and ABPA. Differential diagnosis of ABPA was even possible among cystic fibrosis as well as noncystic fibrosis patients. In the group of immunocompromised patients with IA, no antibody response was mounted in response to the
Aspergillus infection in any of the patients. Interestingly, about half of the patients with proven IA came to the hospital with high titers of anti-
Aspergillus antibodies, suggesting that they were infected upon entry to the hospital. These results suggest that recombinant RNU, CAT, and DPPV have a great potential in the serodiagnosis of all forms of aspergillosis in the immunocompromised and immunocompetent patient.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16626916</pmid><doi>10.1016/j.diagmicrobio.2006.02.002</doi><tpages>13</tpages></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 2006-08, Vol.55 (4), p.279-291 |
| issn | 0732-8893 1879-0070 |
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| subjects | Antigens Antigens, Bacterial - biosynthesis Antigens, Bacterial - immunology Aspergillosis Aspergillosis - diagnosis Aspergillosis - immunology Aspergillus fumigatus Aspergillus fumigatus - genetics Aspergillus fumigatus - immunology Biological and medical sciences Catalase Cystic Fibrosis - immunology Cystic Fibrosis - microbiology Dipeptidylpeptidase ELISA Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Genetic Vectors - biosynthesis Humans Infectious diseases Medical sciences Microbiology Miscellaneous Mycology Pichia Predictive Value of Tests Recombinant Proteins - immunology Ribonuclease |
| title | Recombinant antigens as diagnostic markers for aspergillosis |
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