Clinical Applications of Whole-Blood PCR with Real-Time Instrumentation

As the genetic basis of many human diseases is being discovered, there is increasing need for the detection of single-nucleotide polymorphisms/mutations in medical laboratories. We describe an innovative approach that combines PCR amplification directly on whole blood and real-time detection PCR tec...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2005-11, Vol.51 (11), p.2025-2030
Hauptverfasser:	Castley, Alison, Higgins, Melinda, Ivey, John, Mamotte, Cyril, Sayer, David C, Christiansen, Frank T
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Blood Blood Specimen Collection Deoxyribonucleic acid DNA DNA polymerase Energy transfer Factor V - genetics Fluorescence Resonance Energy Transfer Fundamental and applied biological sciences. Psychology Genotype Genotypes Hemochromatosis Protein Histocompatibility Antigens Class I - genetics Humans Instrumentation Investigative techniques, diagnostic techniques (general aspects) Medical laboratories Medical sciences Membrane Proteins - genetics Methods Mutation Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods Probes Prothrombin - genetics Temperature
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