Effects of a hypoproteic diet on myosin-V immunostained myenteric neurons and the proximal colon wall of aging rats
The objective of this work was to analyze the morphoquantitative behavior of neurons of the myenteric plexus, as well as the morphometry of elements of the proximal colon wall of Wistar rats ( Rattus norvegicus) fed a normoproteic (22%) and a hypoproteic (8%) diet, and sacrificed at 360 days of age....
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| Veröffentlicht in: | Autonomic neuroscience 2005-10, Vol.122 (1), p.77-83 |
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| creator | Schoffen, João Paulo Ferreira Soares, Angélica de Freitas, Priscila Buttow, Nilza Cristina Natali, Maria Raquel Marcal |
| description | The objective of this work was to analyze the morphoquantitative behavior of neurons of the myenteric plexus, as well as the morphometry of elements of the proximal colon wall of Wistar rats (
Rattus norvegicus) fed a normoproteic (22%) and a hypoproteic (8%) diet, and sacrificed at 360 days of age. To perform the neuronal evaluation, we used whole-mount preparations of the proximal colon immunostained with the antibody anti-myosin-V. The neurons were quantified in 80 microscopic fields (16.98 mm
2/animal). The neuronal cell body morphometry was performed in 100 neurons/animal. Samples of the proximal colon were weighed and measured, and then submitted to routine histological processing. They were later stained using the hematoxylin–eosin method in order to carry out morphometric analysis on the mucosa and external muscular layers. The number of neurons and the neuronal cell body morphometry did not present significant differences between the studied groups. A significant reduction in the weight and length of the proximal colon and in mucosa layer thickness was observed in the animals fed with the hypoproteic diet. We concluded that the neuronal and non-neuronal components of the proximal colon adapted to the imposed nutritional condition, which guaranteed the maintenance of their functions. |
| doi_str_mv | 10.1016/j.autneu.2005.08.005 |
| format | Article |
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Rattus norvegicus) fed a normoproteic (22%) and a hypoproteic (8%) diet, and sacrificed at 360 days of age. To perform the neuronal evaluation, we used whole-mount preparations of the proximal colon immunostained with the antibody anti-myosin-V. The neurons were quantified in 80 microscopic fields (16.98 mm
2/animal). The neuronal cell body morphometry was performed in 100 neurons/animal. Samples of the proximal colon were weighed and measured, and then submitted to routine histological processing. They were later stained using the hematoxylin–eosin method in order to carry out morphometric analysis on the mucosa and external muscular layers. The number of neurons and the neuronal cell body morphometry did not present significant differences between the studied groups. A significant reduction in the weight and length of the proximal colon and in mucosa layer thickness was observed in the animals fed with the hypoproteic diet. We concluded that the neuronal and non-neuronal components of the proximal colon adapted to the imposed nutritional condition, which guaranteed the maintenance of their functions.</description><identifier>ISSN: 1566-0702</identifier><identifier>EISSN: 1872-7484</identifier><identifier>DOI: 10.1016/j.autneu.2005.08.005</identifier><identifier>PMID: 16199208</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Age Factors ; Aging ; Animals ; Biological and medical sciences ; Colon - innervation ; Fundamental and applied biological sciences. Psychology ; Hypoproteic diet ; Immunohistochemistry ; Intestinal morphometry ; Intestinal Mucosa - innervation ; Male ; Myenteric neurons ; Myenteric Plexus - cytology ; Myenteric Plexus - metabolism ; Myosin Type V - metabolism ; Myosin-V ; Neurons - cytology ; Neurons - metabolism ; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ ; Protein Deficiency - physiopathology ; Proximal colon ; Rats ; Rats, Wistar ; Vertebrates: nervous system and sense organs</subject><ispartof>Autonomic neuroscience, 2005-10, Vol.122 (1), p.77-83</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-ec7bbc42c554a3dd734a78c4be452767d17695e195528b76c5fe9e3175c2e84a3</citedby><cites>FETCH-LOGICAL-c390t-ec7bbc42c554a3dd734a78c4be452767d17695e195528b76c5fe9e3175c2e84a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1566070205001712$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17217997$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16199208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schoffen, João Paulo Ferreira</creatorcontrib><creatorcontrib>Soares, Angélica</creatorcontrib><creatorcontrib>de Freitas, Priscila</creatorcontrib><creatorcontrib>Buttow, Nilza Cristina</creatorcontrib><creatorcontrib>Natali, Maria Raquel Marcal</creatorcontrib><title>Effects of a hypoproteic diet on myosin-V immunostained myenteric neurons and the proximal colon wall of aging rats</title><title>Autonomic neuroscience</title><addtitle>Auton Neurosci</addtitle><description>The objective of this work was to analyze the morphoquantitative behavior of neurons of the myenteric plexus, as well as the morphometry of elements of the proximal colon wall of Wistar rats (
Rattus norvegicus) fed a normoproteic (22%) and a hypoproteic (8%) diet, and sacrificed at 360 days of age. To perform the neuronal evaluation, we used whole-mount preparations of the proximal colon immunostained with the antibody anti-myosin-V. The neurons were quantified in 80 microscopic fields (16.98 mm
2/animal). The neuronal cell body morphometry was performed in 100 neurons/animal. Samples of the proximal colon were weighed and measured, and then submitted to routine histological processing. They were later stained using the hematoxylin–eosin method in order to carry out morphometric analysis on the mucosa and external muscular layers. The number of neurons and the neuronal cell body morphometry did not present significant differences between the studied groups. A significant reduction in the weight and length of the proximal colon and in mucosa layer thickness was observed in the animals fed with the hypoproteic diet. We concluded that the neuronal and non-neuronal components of the proximal colon adapted to the imposed nutritional condition, which guaranteed the maintenance of their functions.</description><subject>Age Factors</subject><subject>Aging</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Colon - innervation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hypoproteic diet</subject><subject>Immunohistochemistry</subject><subject>Intestinal morphometry</subject><subject>Intestinal Mucosa - innervation</subject><subject>Male</subject><subject>Myenteric neurons</subject><subject>Myenteric Plexus - cytology</subject><subject>Myenteric Plexus - metabolism</subject><subject>Myosin Type V - metabolism</subject><subject>Myosin-V</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</subject><subject>Protein Deficiency - physiopathology</subject><subject>Proximal colon</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>1566-0702</issn><issn>1872-7484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhSNERUvhHyDkDewSbI-fGyRUlYdUiU3L1nLsm9ajxB5shzL_vh5mpO5YXcv6zvG5x133juCBYCI-bQe71gjrQDHmA1ZDGy-6C6Ik7SVT7GU7cyF6LDE9716XssUYK6zFq-6cCKI1xeqiK9fTBK4WlCZk0cN-l3Y5VQgO-QAVpYiWfSoh9r9QWJY1plJtiODbNcQKuYEtQ06xIBs9qg-AmsHfsNgZuTQ3_aOd53_u9yHeo2xredOdTXYu8PY0L7u7r9e3V9_7m5_fflx9uendRuPag5Pj6Bh1nDO78V5umJXKsREYp1JIT6TQHIjmnKpRCscn0LAhkjsKqkkuu49H35bo9wqlmiUUB_NsI6S1GKEkwZzJBrIj6HIqJcNkdrltkPeGYHMo22zNsWxzKNtgZdposvcn_3VcwD-LTu024MMJsMXZeco2ulCeOUmJ1Prw_ucjB62NPwGyKS5AdOBDbp9jfAr_T_IEKvag7w</recordid><startdate>20051030</startdate><enddate>20051030</enddate><creator>Schoffen, João Paulo Ferreira</creator><creator>Soares, Angélica</creator><creator>de Freitas, Priscila</creator><creator>Buttow, Nilza Cristina</creator><creator>Natali, Maria Raquel Marcal</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051030</creationdate><title>Effects of a hypoproteic diet on myosin-V immunostained myenteric neurons and the proximal colon wall of aging rats</title><author>Schoffen, João Paulo Ferreira ; Soares, Angélica ; de Freitas, Priscila ; Buttow, Nilza Cristina ; Natali, Maria Raquel Marcal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-ec7bbc42c554a3dd734a78c4be452767d17695e195528b76c5fe9e3175c2e84a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Age Factors</topic><topic>Aging</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Colon - innervation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hypoproteic diet</topic><topic>Immunohistochemistry</topic><topic>Intestinal morphometry</topic><topic>Intestinal Mucosa - innervation</topic><topic>Male</topic><topic>Myenteric neurons</topic><topic>Myenteric Plexus - cytology</topic><topic>Myenteric Plexus - metabolism</topic><topic>Myosin Type V - metabolism</topic><topic>Myosin-V</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</topic><topic>Protein Deficiency - physiopathology</topic><topic>Proximal colon</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schoffen, João Paulo Ferreira</creatorcontrib><creatorcontrib>Soares, Angélica</creatorcontrib><creatorcontrib>de Freitas, Priscila</creatorcontrib><creatorcontrib>Buttow, Nilza Cristina</creatorcontrib><creatorcontrib>Natali, Maria Raquel Marcal</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Autonomic neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schoffen, João Paulo Ferreira</au><au>Soares, Angélica</au><au>de Freitas, Priscila</au><au>Buttow, Nilza Cristina</au><au>Natali, Maria Raquel Marcal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of a hypoproteic diet on myosin-V immunostained myenteric neurons and the proximal colon wall of aging rats</atitle><jtitle>Autonomic neuroscience</jtitle><addtitle>Auton Neurosci</addtitle><date>2005-10-30</date><risdate>2005</risdate><volume>122</volume><issue>1</issue><spage>77</spage><epage>83</epage><pages>77-83</pages><issn>1566-0702</issn><eissn>1872-7484</eissn><abstract>The objective of this work was to analyze the morphoquantitative behavior of neurons of the myenteric plexus, as well as the morphometry of elements of the proximal colon wall of Wistar rats (
Rattus norvegicus) fed a normoproteic (22%) and a hypoproteic (8%) diet, and sacrificed at 360 days of age. To perform the neuronal evaluation, we used whole-mount preparations of the proximal colon immunostained with the antibody anti-myosin-V. The neurons were quantified in 80 microscopic fields (16.98 mm
2/animal). The neuronal cell body morphometry was performed in 100 neurons/animal. Samples of the proximal colon were weighed and measured, and then submitted to routine histological processing. They were later stained using the hematoxylin–eosin method in order to carry out morphometric analysis on the mucosa and external muscular layers. The number of neurons and the neuronal cell body morphometry did not present significant differences between the studied groups. A significant reduction in the weight and length of the proximal colon and in mucosa layer thickness was observed in the animals fed with the hypoproteic diet. We concluded that the neuronal and non-neuronal components of the proximal colon adapted to the imposed nutritional condition, which guaranteed the maintenance of their functions.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16199208</pmid><doi>10.1016/j.autneu.2005.08.005</doi><tpages>7</tpages></addata></record> |
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| subjects | Age Factors Aging Animals Biological and medical sciences Colon - innervation Fundamental and applied biological sciences. Psychology Hypoproteic diet Immunohistochemistry Intestinal morphometry Intestinal Mucosa - innervation Male Myenteric neurons Myenteric Plexus - cytology Myenteric Plexus - metabolism Myosin Type V - metabolism Myosin-V Neurons - cytology Neurons - metabolism Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ Protein Deficiency - physiopathology Proximal colon Rats Rats, Wistar Vertebrates: nervous system and sense organs |
| title | Effects of a hypoproteic diet on myosin-V immunostained myenteric neurons and the proximal colon wall of aging rats |
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