Expression and localization of two isoforms of AQP10 in human small intestine
Background information. AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different ter...
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| Veröffentlicht in: | Biology of the cell 2005-11, Vol.97 (11), p.823-829 |
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| container_title | Biology of the cell |
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| creator | Li, Huiping Kamiie, Junichi Morishita, Yoshiyuki Yoshida, Yukata Yaoita, Eishin Ishibashi, Kenichi Yamamoto, Tadashi |
| description | Background information. AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different termination of amino acid translation and synthesis of a shorter form of AQP10. Thus an AQP10 molecule deduced from the transcript with an insertion has a distinct C‐terminus, different from that translated from the transcript without the insertion. In the present study, we investigated the expression and localization of these AQP10 isoforms.
Results. Two transcripts were expressed in human duodenum and jejunum. Western‐blot analysis using antibodies for these AQP10 isoforms immunoblotted two bands of approx. 35 and 30 kDa respectively. Immunohistochemistry and immunoelectron microscopy demonstrated localization of the AQP10 with an insertion in the capillary endothelium in villi of the small intestine and the isoform without the insertion in the gastro‐entero‐pancrestic endocrine cells.
Conclusion. The different forms of localization of the AQP10 isoforms indicate that they may play different roles in the proximal portion of human small intestine. |
| doi_str_mv | 10.1042/BC20040091 |
| format | Article |
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Results. Two transcripts were expressed in human duodenum and jejunum. Western‐blot analysis using antibodies for these AQP10 isoforms immunoblotted two bands of approx. 35 and 30 kDa respectively. Immunohistochemistry and immunoelectron microscopy demonstrated localization of the AQP10 with an insertion in the capillary endothelium in villi of the small intestine and the isoform without the insertion in the gastro‐entero‐pancrestic endocrine cells.
Conclusion. The different forms of localization of the AQP10 isoforms indicate that they may play different roles in the proximal portion of human small intestine.</description><identifier>ISSN: 0248-4900</identifier><identifier>EISSN: 1768-322X</identifier><identifier>DOI: 10.1042/BC20040091</identifier><identifier>PMID: 15898950</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; aquaporin ; Aquaporins - biosynthesis ; Blotting, Western ; Duodenum - metabolism ; Endocrine Glands - cytology ; endothelium ; gastro-entero-pancrestic (GEP) ; gastrointestinal tract ; Gene Expression Profiling ; Humans ; Immunohistochemistry ; Intestinal Mucosa - metabolism ; Jejunum - metabolism ; Microscopy, Immunoelectron ; Molecular Sequence Data ; Pancreas - cytology ; Protein Isoforms - biosynthesis ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; water channel</subject><ispartof>Biology of the cell, 2005-11, Vol.97 (11), p.823-829</ispartof><rights>2005 Société Française des Microscopies and Société Biologie Cellulaire de France</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4643-22a1b99f5e4761294698dd822e0ffb62131834d9ddabd82398e753ff5ebf44953</citedby><cites>FETCH-LOGICAL-c4643-22a1b99f5e4761294698dd822e0ffb62131834d9ddabd82398e753ff5ebf44953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1042%2FBC20040091$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1042%2FBC20040091$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15898950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Huiping</creatorcontrib><creatorcontrib>Kamiie, Junichi</creatorcontrib><creatorcontrib>Morishita, Yoshiyuki</creatorcontrib><creatorcontrib>Yoshida, Yukata</creatorcontrib><creatorcontrib>Yaoita, Eishin</creatorcontrib><creatorcontrib>Ishibashi, Kenichi</creatorcontrib><creatorcontrib>Yamamoto, Tadashi</creatorcontrib><title>Expression and localization of two isoforms of AQP10 in human small intestine</title><title>Biology of the cell</title><addtitle>Biol Cell</addtitle><description>Background information. AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different termination of amino acid translation and synthesis of a shorter form of AQP10. Thus an AQP10 molecule deduced from the transcript with an insertion has a distinct C‐terminus, different from that translated from the transcript without the insertion. In the present study, we investigated the expression and localization of these AQP10 isoforms.
Results. Two transcripts were expressed in human duodenum and jejunum. Western‐blot analysis using antibodies for these AQP10 isoforms immunoblotted two bands of approx. 35 and 30 kDa respectively. Immunohistochemistry and immunoelectron microscopy demonstrated localization of the AQP10 with an insertion in the capillary endothelium in villi of the small intestine and the isoform without the insertion in the gastro‐entero‐pancrestic endocrine cells.
Conclusion. The different forms of localization of the AQP10 isoforms indicate that they may play different roles in the proximal portion of human small intestine.</description><subject>Amino Acid Sequence</subject><subject>aquaporin</subject><subject>Aquaporins - biosynthesis</subject><subject>Blotting, Western</subject><subject>Duodenum - metabolism</subject><subject>Endocrine Glands - cytology</subject><subject>endothelium</subject><subject>gastro-entero-pancrestic (GEP)</subject><subject>gastrointestinal tract</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Jejunum - metabolism</subject><subject>Microscopy, Immunoelectron</subject><subject>Molecular Sequence Data</subject><subject>Pancreas - cytology</subject><subject>Protein Isoforms - biosynthesis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Alignment</subject><subject>water channel</subject><issn>0248-4900</issn><issn>1768-322X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPwzAQhC0EoqVw4QegnDggpfiVxD62VVsehQICwc1yElsYkrjEqdry63GVCm6cVrP6ZlY7AJwi2EeQ4svhCENIIeRoD3RRErOQYPy2D7oQUxZSDmEHHDn3AT3FWXQIOihinPEIdsHdeL2olXPGVoGs8qCwmSzMt2y2C6uDZmUD46y2dem2evD4gGBgquB9WcoqcKUsCi8b5RpTqWNwoGXh1Mlu9sDLZPw8ugpn8-n1aDALMxpTEmIsUcq5jhRNYoQ5jTnLc4axglqnMUYEMUJznucy9WvCmUoioj2fakp5RHrgvM1d1PZr6W-L0rhMFYWslF06EbME-g-pBy9aMKutc7XSYlGbUtYbgaDYlif-yvPw2S51mZYq_0N3bXmg3wIrU6jNP1FiOB_FmHhD2BqMa9T61yDrTxEnJInE6_1UoCd0Q-EEi1vyAxU3hNU</recordid><startdate>200511</startdate><enddate>200511</enddate><creator>Li, Huiping</creator><creator>Kamiie, Junichi</creator><creator>Morishita, Yoshiyuki</creator><creator>Yoshida, Yukata</creator><creator>Yaoita, Eishin</creator><creator>Ishibashi, Kenichi</creator><creator>Yamamoto, Tadashi</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200511</creationdate><title>Expression and localization of two isoforms of AQP10 in human small intestine</title><author>Li, Huiping ; Kamiie, Junichi ; Morishita, Yoshiyuki ; Yoshida, Yukata ; Yaoita, Eishin ; Ishibashi, Kenichi ; Yamamoto, Tadashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4643-22a1b99f5e4761294698dd822e0ffb62131834d9ddabd82398e753ff5ebf44953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>aquaporin</topic><topic>Aquaporins - biosynthesis</topic><topic>Blotting, Western</topic><topic>Duodenum - metabolism</topic><topic>Endocrine Glands - cytology</topic><topic>endothelium</topic><topic>gastro-entero-pancrestic (GEP)</topic><topic>gastrointestinal tract</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Jejunum - metabolism</topic><topic>Microscopy, Immunoelectron</topic><topic>Molecular Sequence Data</topic><topic>Pancreas - cytology</topic><topic>Protein Isoforms - biosynthesis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Alignment</topic><topic>water channel</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Huiping</creatorcontrib><creatorcontrib>Kamiie, Junichi</creatorcontrib><creatorcontrib>Morishita, Yoshiyuki</creatorcontrib><creatorcontrib>Yoshida, Yukata</creatorcontrib><creatorcontrib>Yaoita, Eishin</creatorcontrib><creatorcontrib>Ishibashi, Kenichi</creatorcontrib><creatorcontrib>Yamamoto, Tadashi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Huiping</au><au>Kamiie, Junichi</au><au>Morishita, Yoshiyuki</au><au>Yoshida, Yukata</au><au>Yaoita, Eishin</au><au>Ishibashi, Kenichi</au><au>Yamamoto, Tadashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and localization of two isoforms of AQP10 in human small intestine</atitle><jtitle>Biology of the cell</jtitle><addtitle>Biol Cell</addtitle><date>2005-11</date><risdate>2005</risdate><volume>97</volume><issue>11</issue><spage>823</spage><epage>829</epage><pages>823-829</pages><issn>0248-4900</issn><eissn>1768-322X</eissn><abstract>Background information. AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different termination of amino acid translation and synthesis of a shorter form of AQP10. Thus an AQP10 molecule deduced from the transcript with an insertion has a distinct C‐terminus, different from that translated from the transcript without the insertion. In the present study, we investigated the expression and localization of these AQP10 isoforms.
Results. Two transcripts were expressed in human duodenum and jejunum. Western‐blot analysis using antibodies for these AQP10 isoforms immunoblotted two bands of approx. 35 and 30 kDa respectively. Immunohistochemistry and immunoelectron microscopy demonstrated localization of the AQP10 with an insertion in the capillary endothelium in villi of the small intestine and the isoform without the insertion in the gastro‐entero‐pancrestic endocrine cells.
Conclusion. The different forms of localization of the AQP10 isoforms indicate that they may play different roles in the proximal portion of human small intestine.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>15898950</pmid><doi>10.1042/BC20040091</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biology of the cell, 2005-11, Vol.97 (11), p.823-829 |
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| source | MEDLINE; Wiley Journals |
| subjects | Amino Acid Sequence aquaporin Aquaporins - biosynthesis Blotting, Western Duodenum - metabolism Endocrine Glands - cytology endothelium gastro-entero-pancrestic (GEP) gastrointestinal tract Gene Expression Profiling Humans Immunohistochemistry Intestinal Mucosa - metabolism Jejunum - metabolism Microscopy, Immunoelectron Molecular Sequence Data Pancreas - cytology Protein Isoforms - biosynthesis Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment water channel |
| title | Expression and localization of two isoforms of AQP10 in human small intestine |
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