Characterizing a stable methane-utilizing mixed culture used in the synthesis of a high-quality biopolymer in an open system
To characterize a methane-utilizing poly-β-hydroxybutyrate (PHB)-producing microbial community. Three different approaches based on microbiology, analytical chemistry and molecular biology were used to determine the composition of the mixed culture. The dominant species, Methylocystis sp. GB25, repr...
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description | To characterize a methane-utilizing poly-β-hydroxybutyrate (PHB)-producing microbial community. Three different approaches based on microbiology, analytical chemistry and molecular biology were used to determine the composition of the mixed culture. The dominant species, Methylocystis sp. GB25, represents more than 86% of the total biomass. Seven accompanying bacterial species are present in the mixed culture of which two are methylotrophic bacteria and five are utilizers of complex carbon sources. Both these groups were found to be present at the same ratio with respect to each other. Results of fatty acid analysis and PCR-DGGE fingerprints reflect the stability of the mixed-culture composition in the open system during multiple continuous growth and polymer formation processes throughout a period of 29 months. The consistently high quality of the accumulated polymer further corroborates this finding. The methane-utilizing mixed culture has the potential of self-regulation resulting in a stable composition even under non-aseptic conditions. Avoiding the necessity of sterile conditions, as demonstrated in this paper, is an important step towards the development of a viable large-scale process for the production of PHB using cheap substrates like methane from natural or renewable sources. This is the first report characterizing a bacterial mixed culture being used for the biotechnological production of a high-value product in an open system. |
doi_str_mv | 10.1111/j.1365-2672.2006.02960.x |
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Three different approaches based on microbiology, analytical chemistry and molecular biology were used to determine the composition of the mixed culture. The dominant species, Methylocystis sp. GB25, represents more than 86% of the total biomass. Seven accompanying bacterial species are present in the mixed culture of which two are methylotrophic bacteria and five are utilizers of complex carbon sources. Both these groups were found to be present at the same ratio with respect to each other. Results of fatty acid analysis and PCR-DGGE fingerprints reflect the stability of the mixed-culture composition in the open system during multiple continuous growth and polymer formation processes throughout a period of 29 months. The consistently high quality of the accumulated polymer further corroborates this finding. The methane-utilizing mixed culture has the potential of self-regulation resulting in a stable composition even under non-aseptic conditions. Avoiding the necessity of sterile conditions, as demonstrated in this paper, is an important step towards the development of a viable large-scale process for the production of PHB using cheap substrates like methane from natural or renewable sources. This is the first report characterizing a bacterial mixed culture being used for the biotechnological production of a high-value product in an open system.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2006.02960.x</identifier><identifier>PMID: 16882146</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Bacteria - chemistry ; Bacteria - isolation & purification ; Bacteria - metabolism ; Biological and medical sciences ; Biomass ; biopolymer ; Biopolymers ; Bioreactors ; Colony Count, Microbial ; Fatty Acids - analysis ; Fundamental and applied biological sciences. Psychology ; Hydroxybutyrates - metabolism ; Methane - metabolism ; methane‐utilizing mixed culture ; Methylocystaceae - chemistry ; Methylocystaceae - isolation & purification ; Methylocystaceae - metabolism ; Methylocystis ; Methylocystis sp. GB25 ; Microbiology ; non‐aseptic ; open system ; Polyesters - metabolism ; poly‐β‐hydroxybutyrate (PHB)</subject><ispartof>Journal of applied microbiology, 2006-08, Vol.101 (2), p.387-395</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5680-7d48d86e73574a92c593dcdd74d0579da94a9b90eb0bcccde1ee2853d0a9f4943</citedby><cites>FETCH-LOGICAL-c5680-7d48d86e73574a92c593dcdd74d0579da94a9b90eb0bcccde1ee2853d0a9f4943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2006.02960.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2006.02960.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17965149$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16882146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Helm, J</creatorcontrib><creatorcontrib>Wendlandt, K.-D</creatorcontrib><creatorcontrib>Rogge, G</creatorcontrib><creatorcontrib>Kappelmeyer, U</creatorcontrib><title>Characterizing a stable methane-utilizing mixed culture used in the synthesis of a high-quality biopolymer in an open system</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>To characterize a methane-utilizing poly-β-hydroxybutyrate (PHB)-producing microbial community. Three different approaches based on microbiology, analytical chemistry and molecular biology were used to determine the composition of the mixed culture. The dominant species, Methylocystis sp. GB25, represents more than 86% of the total biomass. Seven accompanying bacterial species are present in the mixed culture of which two are methylotrophic bacteria and five are utilizers of complex carbon sources. Both these groups were found to be present at the same ratio with respect to each other. Results of fatty acid analysis and PCR-DGGE fingerprints reflect the stability of the mixed-culture composition in the open system during multiple continuous growth and polymer formation processes throughout a period of 29 months. The consistently high quality of the accumulated polymer further corroborates this finding. The methane-utilizing mixed culture has the potential of self-regulation resulting in a stable composition even under non-aseptic conditions. Avoiding the necessity of sterile conditions, as demonstrated in this paper, is an important step towards the development of a viable large-scale process for the production of PHB using cheap substrates like methane from natural or renewable sources. This is the first report characterizing a bacterial mixed culture being used for the biotechnological production of a high-value product in an open system.</description><subject>Bacteria - chemistry</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>biopolymer</subject><subject>Biopolymers</subject><subject>Bioreactors</subject><subject>Colony Count, Microbial</subject><subject>Fatty Acids - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydroxybutyrates - metabolism</subject><subject>Methane - metabolism</subject><subject>methane‐utilizing mixed culture</subject><subject>Methylocystaceae - chemistry</subject><subject>Methylocystaceae - isolation & purification</subject><subject>Methylocystaceae - metabolism</subject><subject>Methylocystis</subject><subject>Methylocystis sp. GB25</subject><subject>Microbiology</subject><subject>non‐aseptic</subject><subject>open system</subject><subject>Polyesters - metabolism</subject><subject>poly‐β‐hydroxybutyrate (PHB)</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2P1SAUhhujccbRv6BsdNcKlEJZuJjc-JkxLnTWhMLpLTe0vQNtvDX-eOn0xlkqm3PgPO85kJcsQwQXJK23h4KUvMopF7SgGPMCU8lxcXqUXf4tPL7PWV5hQS-yZzEeMCYlrvjT7ILwuqaE8cvs967TQZsJgvvlhj3SKE668YB6mDo9QD5Pzm-l3p3AIjP7aQ6A5pg2bkBTByguQwrRRTS2qUPn9l1-N2vvpgU1bjyOfukhrLQe0HiEISniBP3z7EmrfYQX53iV3X54_2P3Kb_59vHz7vomNxWvcS4sq23NQZSVYFpSU8nSGmsFs7gS0mqZThuJocGNMcYCAaB1VVqsZcskK6-yN1vfYxjvZoiT6l004H164DhHxWuBWUnwP0EiS1pTtnasN9CEMcYArToG1-uwKILVapE6qNUJtTqhVovUvUXqlKQvzzPmpgf7IDx7koDXZ0BHo30b9GBcfOCE5BVhMnHvNu6n87D89wXUl-uva5b0rzZ9q0el9yHNuP1O1z9CcC1oxcs_itu30w</recordid><startdate>200608</startdate><enddate>200608</enddate><creator>Helm, J</creator><creator>Wendlandt, K.-D</creator><creator>Rogge, G</creator><creator>Kappelmeyer, U</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200608</creationdate><title>Characterizing a stable methane-utilizing mixed culture used in the synthesis of a high-quality biopolymer in an open system</title><author>Helm, J ; Wendlandt, K.-D ; Rogge, G ; Kappelmeyer, U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5680-7d48d86e73574a92c593dcdd74d0579da94a9b90eb0bcccde1ee2853d0a9f4943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacteria - chemistry</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteria - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>biopolymer</topic><topic>Biopolymers</topic><topic>Bioreactors</topic><topic>Colony Count, Microbial</topic><topic>Fatty Acids - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydroxybutyrates - metabolism</topic><topic>Methane - metabolism</topic><topic>methane‐utilizing mixed culture</topic><topic>Methylocystaceae - chemistry</topic><topic>Methylocystaceae - isolation & purification</topic><topic>Methylocystaceae - metabolism</topic><topic>Methylocystis</topic><topic>Methylocystis sp. GB25</topic><topic>Microbiology</topic><topic>non‐aseptic</topic><topic>open system</topic><topic>Polyesters - metabolism</topic><topic>poly‐β‐hydroxybutyrate (PHB)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Helm, J</creatorcontrib><creatorcontrib>Wendlandt, K.-D</creatorcontrib><creatorcontrib>Rogge, G</creatorcontrib><creatorcontrib>Kappelmeyer, U</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Helm, J</au><au>Wendlandt, K.-D</au><au>Rogge, G</au><au>Kappelmeyer, U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterizing a stable methane-utilizing mixed culture used in the synthesis of a high-quality biopolymer in an open system</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2006-08</date><risdate>2006</risdate><volume>101</volume><issue>2</issue><spage>387</spage><epage>395</epage><pages>387-395</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>To characterize a methane-utilizing poly-β-hydroxybutyrate (PHB)-producing microbial community. Three different approaches based on microbiology, analytical chemistry and molecular biology were used to determine the composition of the mixed culture. The dominant species, Methylocystis sp. GB25, represents more than 86% of the total biomass. Seven accompanying bacterial species are present in the mixed culture of which two are methylotrophic bacteria and five are utilizers of complex carbon sources. Both these groups were found to be present at the same ratio with respect to each other. Results of fatty acid analysis and PCR-DGGE fingerprints reflect the stability of the mixed-culture composition in the open system during multiple continuous growth and polymer formation processes throughout a period of 29 months. The consistently high quality of the accumulated polymer further corroborates this finding. The methane-utilizing mixed culture has the potential of self-regulation resulting in a stable composition even under non-aseptic conditions. Avoiding the necessity of sterile conditions, as demonstrated in this paper, is an important step towards the development of a viable large-scale process for the production of PHB using cheap substrates like methane from natural or renewable sources. This is the first report characterizing a bacterial mixed culture being used for the biotechnological production of a high-value product in an open system.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>16882146</pmid><doi>10.1111/j.1365-2672.2006.02960.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Bacteria - chemistry Bacteria - isolation & purification Bacteria - metabolism Biological and medical sciences Biomass biopolymer Biopolymers Bioreactors Colony Count, Microbial Fatty Acids - analysis Fundamental and applied biological sciences. Psychology Hydroxybutyrates - metabolism Methane - metabolism methane‐utilizing mixed culture Methylocystaceae - chemistry Methylocystaceae - isolation & purification Methylocystaceae - metabolism Methylocystis Methylocystis sp. GB25 Microbiology non‐aseptic open system Polyesters - metabolism poly‐β‐hydroxybutyrate (PHB) |
title | Characterizing a stable methane-utilizing mixed culture used in the synthesis of a high-quality biopolymer in an open system |
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