Characterizing a stable methane-utilizing mixed culture used in the synthesis of a high-quality biopolymer in an open system

To characterize a methane-utilizing poly-β-hydroxybutyrate (PHB)-producing microbial community. Three different approaches based on microbiology, analytical chemistry and molecular biology were used to determine the composition of the mixed culture. The dominant species, Methylocystis sp. GB25, repr...

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Veröffentlicht in:Journal of applied microbiology 2006-08, Vol.101 (2), p.387-395
Hauptverfasser: Helm, J, Wendlandt, K.-D, Rogge, G, Kappelmeyer, U
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container_title Journal of applied microbiology
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creator Helm, J
Wendlandt, K.-D
Rogge, G
Kappelmeyer, U
description To characterize a methane-utilizing poly-β-hydroxybutyrate (PHB)-producing microbial community. Three different approaches based on microbiology, analytical chemistry and molecular biology were used to determine the composition of the mixed culture. The dominant species, Methylocystis sp. GB25, represents more than 86% of the total biomass. Seven accompanying bacterial species are present in the mixed culture of which two are methylotrophic bacteria and five are utilizers of complex carbon sources. Both these groups were found to be present at the same ratio with respect to each other. Results of fatty acid analysis and PCR-DGGE fingerprints reflect the stability of the mixed-culture composition in the open system during multiple continuous growth and polymer formation processes throughout a period of 29 months. The consistently high quality of the accumulated polymer further corroborates this finding. The methane-utilizing mixed culture has the potential of self-regulation resulting in a stable composition even under non-aseptic conditions. Avoiding the necessity of sterile conditions, as demonstrated in this paper, is an important step towards the development of a viable large-scale process for the production of PHB using cheap substrates like methane from natural or renewable sources. This is the first report characterizing a bacterial mixed culture being used for the biotechnological production of a high-value product in an open system.
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Psychology ; Hydroxybutyrates - metabolism ; Methane - metabolism ; methane‐utilizing mixed culture ; Methylocystaceae - chemistry ; Methylocystaceae - isolation &amp; purification ; Methylocystaceae - metabolism ; Methylocystis ; Methylocystis sp. 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Three different approaches based on microbiology, analytical chemistry and molecular biology were used to determine the composition of the mixed culture. The dominant species, Methylocystis sp. GB25, represents more than 86% of the total biomass. Seven accompanying bacterial species are present in the mixed culture of which two are methylotrophic bacteria and five are utilizers of complex carbon sources. Both these groups were found to be present at the same ratio with respect to each other. Results of fatty acid analysis and PCR-DGGE fingerprints reflect the stability of the mixed-culture composition in the open system during multiple continuous growth and polymer formation processes throughout a period of 29 months. The consistently high quality of the accumulated polymer further corroborates this finding. The methane-utilizing mixed culture has the potential of self-regulation resulting in a stable composition even under non-aseptic conditions. Avoiding the necessity of sterile conditions, as demonstrated in this paper, is an important step towards the development of a viable large-scale process for the production of PHB using cheap substrates like methane from natural or renewable sources. This is the first report characterizing a bacterial mixed culture being used for the biotechnological production of a high-value product in an open system.</description><subject>Bacteria - chemistry</subject><subject>Bacteria - isolation &amp; purification</subject><subject>Bacteria - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>biopolymer</subject><subject>Biopolymers</subject><subject>Bioreactors</subject><subject>Colony Count, Microbial</subject><subject>Fatty Acids - analysis</subject><subject>Fundamental and applied biological sciences. 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source Wiley-Blackwell Journals; MEDLINE; Oxford Academic Journals (OUP)
subjects Bacteria - chemistry
Bacteria - isolation & purification
Bacteria - metabolism
Biological and medical sciences
Biomass
biopolymer
Biopolymers
Bioreactors
Colony Count, Microbial
Fatty Acids - analysis
Fundamental and applied biological sciences. Psychology
Hydroxybutyrates - metabolism
Methane - metabolism
methane‐utilizing mixed culture
Methylocystaceae - chemistry
Methylocystaceae - isolation & purification
Methylocystaceae - metabolism
Methylocystis
Methylocystis sp. GB25
Microbiology
non‐aseptic
open system
Polyesters - metabolism
poly‐β‐hydroxybutyrate (PHB)
title Characterizing a stable methane-utilizing mixed culture used in the synthesis of a high-quality biopolymer in an open system
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