Endocytosis and vesicle trafficking during tip growth of root hairs
The directional elongation of root hairs, "tip growth", depends on the coordinated and highly regulated trafficking of vesicles which fill the tip cytoplasm and are active in secretion of cell wall material. So far, little is known about the dynamics of endocytosis in living root hairs. We...
Gespeichert in:
Veröffentlicht in: | Protoplasma 2005-10, Vol.226 (1-2), p.39-54 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 54 |
---|---|
container_issue | 1-2 |
container_start_page | 39 |
container_title | Protoplasma |
container_volume | 226 |
creator | Ovecka, M Lang, I Baluska, F Ismail, A Illes, P Lichtscheidl, I.K |
description | The directional elongation of root hairs, "tip growth", depends on the coordinated and highly regulated trafficking of vesicles which fill the tip cytoplasm and are active in secretion of cell wall material. So far, little is known about the dynamics of endocytosis in living root hairs. We analyzed the motile behaviour of vesicles in the apical region of living root hairs of Arabidopsis thaliana and of Triticum aestivum by live cell microscopy. For direct observation of endocytosis and of the fate of endocytic vesicles, we used the fluorescent endocytosis marker dyes FM 1-43 and FM 4-64. Rapid endocytosis was detected mainly in the tip, where it caused a bright fluorescence of the apical cytoplasm. The internalized membranes proceeded through highly dynamic putative early endosomes in the clear zone to larger endosomal compartments in the subapical region that are excluded from the clear zone. The internalized cargo ended up in the dynamic vacuole by fusion of large endosomal compartments with the tonoplast. Before export to these lytic compartments, putative early endosomes remained in the apical zone, where they most probably recycled to the plasma membrane and back into the cytoplasm for more than 30 min. Endoplasmic reticulum was not involved in trafficking pathways of endosomes. Actin cytoskeleton was needed for the endocytosis itself, as well as for further membrane trafficking. The actin-depolymerizing drug latrunculin B modified the dynamic properties of vesicles and endosomes; they became immobilized and aggregated in the tip. Treatment with brefeldin A inhibited membrane trafficking and caused the disappearance of FM-containing vesicles and putative early endosomes from the clear zone; labelled structures accumulated in motile brefeldin A-induced compartments. These large endocytic compartments redispersed upon removal of the drug. Our results hence prove that endocytosis occurs in growing root hairs. We show the localization of endocytosis in the tip and indicate specific endomembrane compartments and their recycling. |
doi_str_mv | 10.1007/s00709-005-0103-9 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68703509</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2785610911</sourcerecordid><originalsourceid>FETCH-LOGICAL-c445t-bb7669444a40e8aae5997ab951f1b712b1c2726895d0b10197fd454b853d1d243</originalsourceid><addsrcrecordid>eNp90c1qGzEUBWARGmLH7QN0kwwUupv2Xv2OlsWkTSDQRWLoTmhmNLZce-RIMwl--8jYEMgiG93NpyNxDyFfEX4ggPqZ8gG6BBAlILBSn5EpShSlRKCfyBSAsRIr9m9CLlNaQ4YUxAWZoKQMc8SUzG_6NjT7ISSfCtu3xbNLvtm4Yoi263zz3_fLoh3jYQx-VyxjeBlWReiKGMJQrKyP6TM57-wmuS-nOSOL3zeP89vy_u-fu_mv-7LhXAxlXSspNefccnCVtU5orWytBXZYK6Q1NlRRWWnRQo2AWnUtF7yuBGuxpZzNyPdj7i6Gp9GlwWx9atxmY3sXxmRkpYAJ0Bl-ewfXYYx9_puhUrKKgubsI4WghdRCKZUVHlUTQ0rRdWYX_dbGfUbm0II5tmDycs2hBXN4_-qUPNZb177dOK09g-sj6Gwwdhl9MosHCshygNTIOXsFNvKIxQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1095695777</pqid></control><display><type>article</type><title>Endocytosis and vesicle trafficking during tip growth of root hairs</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Ovecka, M ; Lang, I ; Baluska, F ; Ismail, A ; Illes, P ; Lichtscheidl, I.K</creator><creatorcontrib>Ovecka, M ; Lang, I ; Baluska, F ; Ismail, A ; Illes, P ; Lichtscheidl, I.K</creatorcontrib><description>The directional elongation of root hairs, "tip growth", depends on the coordinated and highly regulated trafficking of vesicles which fill the tip cytoplasm and are active in secretion of cell wall material. So far, little is known about the dynamics of endocytosis in living root hairs. We analyzed the motile behaviour of vesicles in the apical region of living root hairs of Arabidopsis thaliana and of Triticum aestivum by live cell microscopy. For direct observation of endocytosis and of the fate of endocytic vesicles, we used the fluorescent endocytosis marker dyes FM 1-43 and FM 4-64. Rapid endocytosis was detected mainly in the tip, where it caused a bright fluorescence of the apical cytoplasm. The internalized membranes proceeded through highly dynamic putative early endosomes in the clear zone to larger endosomal compartments in the subapical region that are excluded from the clear zone. The internalized cargo ended up in the dynamic vacuole by fusion of large endosomal compartments with the tonoplast. Before export to these lytic compartments, putative early endosomes remained in the apical zone, where they most probably recycled to the plasma membrane and back into the cytoplasm for more than 30 min. Endoplasmic reticulum was not involved in trafficking pathways of endosomes. Actin cytoskeleton was needed for the endocytosis itself, as well as for further membrane trafficking. The actin-depolymerizing drug latrunculin B modified the dynamic properties of vesicles and endosomes; they became immobilized and aggregated in the tip. Treatment with brefeldin A inhibited membrane trafficking and caused the disappearance of FM-containing vesicles and putative early endosomes from the clear zone; labelled structures accumulated in motile brefeldin A-induced compartments. These large endocytic compartments redispersed upon removal of the drug. Our results hence prove that endocytosis occurs in growing root hairs. We show the localization of endocytosis in the tip and indicate specific endomembrane compartments and their recycling.</description><identifier>ISSN: 0033-183X</identifier><identifier>EISSN: 1615-6102</identifier><identifier>DOI: 10.1007/s00709-005-0103-9</identifier><identifier>PMID: 16231100</identifier><language>eng</language><publisher>Austria: Springer Nature B.V</publisher><subject>Actin ; Arabidopsis - cytology ; Arabidopsis - growth & development ; Arabidopsis thaliana ; Brefeldin A ; Brefeldin A - pharmacology ; Bridged Bicyclo Compounds, Heterocyclic - pharmacology ; cell biology ; Cell walls ; Cells ; Cellular biology ; Cytoplasm ; Cytoskeleton ; Endocytosis ; Endocytosis - drug effects ; Endocytosis - physiology ; Endoplasmic reticulum ; Endoplasmic Reticulum - drug effects ; Endosomes ; Latrunculin B ; Localization ; Membrane trafficking ; Microscopy, Fluorescence ; Plant Physiological Phenomena ; Plant Roots - cytology ; Plant Roots - growth & development ; Plant Roots - physiology ; Protein transport ; Protein turnover ; Proteins ; root growth ; Root hairs ; root tips ; Thiazoles - pharmacology ; Thiazolidines ; Transport Vesicles - physiology ; Triticum - cytology ; Triticum - growth & development ; Triticum aestivum ; Vesicles</subject><ispartof>Protoplasma, 2005-10, Vol.226 (1-2), p.39-54</ispartof><rights>Springer-Verlag/Wien 2005</rights><rights>Springer-Verlag/Wien 2005.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-bb7669444a40e8aae5997ab951f1b712b1c2726895d0b10197fd454b853d1d243</citedby><cites>FETCH-LOGICAL-c445t-bb7669444a40e8aae5997ab951f1b712b1c2726895d0b10197fd454b853d1d243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16231100$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ovecka, M</creatorcontrib><creatorcontrib>Lang, I</creatorcontrib><creatorcontrib>Baluska, F</creatorcontrib><creatorcontrib>Ismail, A</creatorcontrib><creatorcontrib>Illes, P</creatorcontrib><creatorcontrib>Lichtscheidl, I.K</creatorcontrib><title>Endocytosis and vesicle trafficking during tip growth of root hairs</title><title>Protoplasma</title><addtitle>Protoplasma</addtitle><description>The directional elongation of root hairs, "tip growth", depends on the coordinated and highly regulated trafficking of vesicles which fill the tip cytoplasm and are active in secretion of cell wall material. So far, little is known about the dynamics of endocytosis in living root hairs. We analyzed the motile behaviour of vesicles in the apical region of living root hairs of Arabidopsis thaliana and of Triticum aestivum by live cell microscopy. For direct observation of endocytosis and of the fate of endocytic vesicles, we used the fluorescent endocytosis marker dyes FM 1-43 and FM 4-64. Rapid endocytosis was detected mainly in the tip, where it caused a bright fluorescence of the apical cytoplasm. The internalized membranes proceeded through highly dynamic putative early endosomes in the clear zone to larger endosomal compartments in the subapical region that are excluded from the clear zone. The internalized cargo ended up in the dynamic vacuole by fusion of large endosomal compartments with the tonoplast. Before export to these lytic compartments, putative early endosomes remained in the apical zone, where they most probably recycled to the plasma membrane and back into the cytoplasm for more than 30 min. Endoplasmic reticulum was not involved in trafficking pathways of endosomes. Actin cytoskeleton was needed for the endocytosis itself, as well as for further membrane trafficking. The actin-depolymerizing drug latrunculin B modified the dynamic properties of vesicles and endosomes; they became immobilized and aggregated in the tip. Treatment with brefeldin A inhibited membrane trafficking and caused the disappearance of FM-containing vesicles and putative early endosomes from the clear zone; labelled structures accumulated in motile brefeldin A-induced compartments. These large endocytic compartments redispersed upon removal of the drug. Our results hence prove that endocytosis occurs in growing root hairs. We show the localization of endocytosis in the tip and indicate specific endomembrane compartments and their recycling.</description><subject>Actin</subject><subject>Arabidopsis - cytology</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis thaliana</subject><subject>Brefeldin A</subject><subject>Brefeldin A - pharmacology</subject><subject>Bridged Bicyclo Compounds, Heterocyclic - pharmacology</subject><subject>cell biology</subject><subject>Cell walls</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Cytoplasm</subject><subject>Cytoskeleton</subject><subject>Endocytosis</subject><subject>Endocytosis - drug effects</subject><subject>Endocytosis - physiology</subject><subject>Endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - drug effects</subject><subject>Endosomes</subject><subject>Latrunculin B</subject><subject>Localization</subject><subject>Membrane trafficking</subject><subject>Microscopy, Fluorescence</subject><subject>Plant Physiological Phenomena</subject><subject>Plant Roots - cytology</subject><subject>Plant Roots - growth & development</subject><subject>Plant Roots - physiology</subject><subject>Protein transport</subject><subject>Protein turnover</subject><subject>Proteins</subject><subject>root growth</subject><subject>Root hairs</subject><subject>root tips</subject><subject>Thiazoles - pharmacology</subject><subject>Thiazolidines</subject><subject>Transport Vesicles - physiology</subject><subject>Triticum - cytology</subject><subject>Triticum - growth & development</subject><subject>Triticum aestivum</subject><subject>Vesicles</subject><issn>0033-183X</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90c1qGzEUBWARGmLH7QN0kwwUupv2Xv2OlsWkTSDQRWLoTmhmNLZce-RIMwl--8jYEMgiG93NpyNxDyFfEX4ggPqZ8gG6BBAlILBSn5EpShSlRKCfyBSAsRIr9m9CLlNaQ4YUxAWZoKQMc8SUzG_6NjT7ISSfCtu3xbNLvtm4Yoi263zz3_fLoh3jYQx-VyxjeBlWReiKGMJQrKyP6TM57-wmuS-nOSOL3zeP89vy_u-fu_mv-7LhXAxlXSspNefccnCVtU5orWytBXZYK6Q1NlRRWWnRQo2AWnUtF7yuBGuxpZzNyPdj7i6Gp9GlwWx9atxmY3sXxmRkpYAJ0Bl-ewfXYYx9_puhUrKKgubsI4WghdRCKZUVHlUTQ0rRdWYX_dbGfUbm0II5tmDycs2hBXN4_-qUPNZb177dOK09g-sj6Gwwdhl9MosHCshygNTIOXsFNvKIxQ</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Ovecka, M</creator><creator>Lang, I</creator><creator>Baluska, F</creator><creator>Ismail, A</creator><creator>Illes, P</creator><creator>Lichtscheidl, I.K</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20051001</creationdate><title>Endocytosis and vesicle trafficking during tip growth of root hairs</title><author>Ovecka, M ; Lang, I ; Baluska, F ; Ismail, A ; Illes, P ; Lichtscheidl, I.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-bb7669444a40e8aae5997ab951f1b712b1c2726895d0b10197fd454b853d1d243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Actin</topic><topic>Arabidopsis - cytology</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis thaliana</topic><topic>Brefeldin A</topic><topic>Brefeldin A - pharmacology</topic><topic>Bridged Bicyclo Compounds, Heterocyclic - pharmacology</topic><topic>cell biology</topic><topic>Cell walls</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>Cytoplasm</topic><topic>Cytoskeleton</topic><topic>Endocytosis</topic><topic>Endocytosis - drug effects</topic><topic>Endocytosis - physiology</topic><topic>Endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - drug effects</topic><topic>Endosomes</topic><topic>Latrunculin B</topic><topic>Localization</topic><topic>Membrane trafficking</topic><topic>Microscopy, Fluorescence</topic><topic>Plant Physiological Phenomena</topic><topic>Plant Roots - cytology</topic><topic>Plant Roots - growth & development</topic><topic>Plant Roots - physiology</topic><topic>Protein transport</topic><topic>Protein turnover</topic><topic>Proteins</topic><topic>root growth</topic><topic>Root hairs</topic><topic>root tips</topic><topic>Thiazoles - pharmacology</topic><topic>Thiazolidines</topic><topic>Transport Vesicles - physiology</topic><topic>Triticum - cytology</topic><topic>Triticum - growth & development</topic><topic>Triticum aestivum</topic><topic>Vesicles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ovecka, M</creatorcontrib><creatorcontrib>Lang, I</creatorcontrib><creatorcontrib>Baluska, F</creatorcontrib><creatorcontrib>Ismail, A</creatorcontrib><creatorcontrib>Illes, P</creatorcontrib><creatorcontrib>Lichtscheidl, I.K</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Protoplasma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ovecka, M</au><au>Lang, I</au><au>Baluska, F</au><au>Ismail, A</au><au>Illes, P</au><au>Lichtscheidl, I.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endocytosis and vesicle trafficking during tip growth of root hairs</atitle><jtitle>Protoplasma</jtitle><addtitle>Protoplasma</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>226</volume><issue>1-2</issue><spage>39</spage><epage>54</epage><pages>39-54</pages><issn>0033-183X</issn><eissn>1615-6102</eissn><abstract>The directional elongation of root hairs, "tip growth", depends on the coordinated and highly regulated trafficking of vesicles which fill the tip cytoplasm and are active in secretion of cell wall material. So far, little is known about the dynamics of endocytosis in living root hairs. We analyzed the motile behaviour of vesicles in the apical region of living root hairs of Arabidopsis thaliana and of Triticum aestivum by live cell microscopy. For direct observation of endocytosis and of the fate of endocytic vesicles, we used the fluorescent endocytosis marker dyes FM 1-43 and FM 4-64. Rapid endocytosis was detected mainly in the tip, where it caused a bright fluorescence of the apical cytoplasm. The internalized membranes proceeded through highly dynamic putative early endosomes in the clear zone to larger endosomal compartments in the subapical region that are excluded from the clear zone. The internalized cargo ended up in the dynamic vacuole by fusion of large endosomal compartments with the tonoplast. Before export to these lytic compartments, putative early endosomes remained in the apical zone, where they most probably recycled to the plasma membrane and back into the cytoplasm for more than 30 min. Endoplasmic reticulum was not involved in trafficking pathways of endosomes. Actin cytoskeleton was needed for the endocytosis itself, as well as for further membrane trafficking. The actin-depolymerizing drug latrunculin B modified the dynamic properties of vesicles and endosomes; they became immobilized and aggregated in the tip. Treatment with brefeldin A inhibited membrane trafficking and caused the disappearance of FM-containing vesicles and putative early endosomes from the clear zone; labelled structures accumulated in motile brefeldin A-induced compartments. These large endocytic compartments redispersed upon removal of the drug. Our results hence prove that endocytosis occurs in growing root hairs. We show the localization of endocytosis in the tip and indicate specific endomembrane compartments and their recycling.</abstract><cop>Austria</cop><pub>Springer Nature B.V</pub><pmid>16231100</pmid><doi>10.1007/s00709-005-0103-9</doi><tpages>16</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0033-183X |
ispartof | Protoplasma, 2005-10, Vol.226 (1-2), p.39-54 |
issn | 0033-183X 1615-6102 |
language | eng |
recordid | cdi_proquest_miscellaneous_68703509 |
source | MEDLINE; SpringerLink Journals |
subjects | Actin Arabidopsis - cytology Arabidopsis - growth & development Arabidopsis thaliana Brefeldin A Brefeldin A - pharmacology Bridged Bicyclo Compounds, Heterocyclic - pharmacology cell biology Cell walls Cells Cellular biology Cytoplasm Cytoskeleton Endocytosis Endocytosis - drug effects Endocytosis - physiology Endoplasmic reticulum Endoplasmic Reticulum - drug effects Endosomes Latrunculin B Localization Membrane trafficking Microscopy, Fluorescence Plant Physiological Phenomena Plant Roots - cytology Plant Roots - growth & development Plant Roots - physiology Protein transport Protein turnover Proteins root growth Root hairs root tips Thiazoles - pharmacology Thiazolidines Transport Vesicles - physiology Triticum - cytology Triticum - growth & development Triticum aestivum Vesicles |
title | Endocytosis and vesicle trafficking during tip growth of root hairs |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T01%3A42%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Endocytosis%20and%20vesicle%20trafficking%20during%20tip%20growth%20of%20root%20hairs&rft.jtitle=Protoplasma&rft.au=Ovecka,%20M&rft.date=2005-10-01&rft.volume=226&rft.issue=1-2&rft.spage=39&rft.epage=54&rft.pages=39-54&rft.issn=0033-183X&rft.eissn=1615-6102&rft_id=info:doi/10.1007/s00709-005-0103-9&rft_dat=%3Cproquest_cross%3E2785610911%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1095695777&rft_id=info:pmid/16231100&rfr_iscdi=true |