Solubilization and refolding of bacterial inclusion body proteins

Inclusion bodies produced in Escherichia coli are composed of densely packed denatured protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive forms is cumbersome, results in poor recovery and accounts for the major cost in production of recombinant proteins f...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2005-04, Vol.99 (4), p.303-310
Hauptverfasser:	Singh, Surinder Mohan, Panda, Amulya Kumar
Format:	Artikel
Sprache:	eng
Schlagworte:	aggregation BACTERIA Biological and medical sciences Biotechnology Cell Fractionation - methods ESCHERICHIA COLI Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Escherichia coli Proteins - isolation & purification Fundamental and applied biological sciences. Psychology HORMONES human growth hormone Human Growth Hormone - chemistry Human Growth Hormone - genetics Human Growth Hormone - isolation & purification Humans Inclusion Bodies - chemistry Inclusion Bodies - metabolism inclusion body native-like secondary structure Protein Denaturation Protein Engineering - methods Protein Folding protein refolding PROTEINS Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Solubility SOLUBILIZATION
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