Age-related changes in neutral sphingomyelin-specific phospholipase C activity in striatum, hippocampus, and frontal cortex: Implication for sensitivity to stress and inflammation
Previous studies show the enrichment of mammalian brain with neutral sphingomyelin-specific phospholipase C (ceramide-phosphocholine phosphodiesterase, EC 3.1.4.12; N-Sase), a key enzyme of sphingolipid metabolism and sphingolipid-induced signaling. The objective of this study was to evaluate the me...
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| creator | Crivello, Natalia A. Rosenberg, Irwin H. Dallal, Gerard E. Bielinski, Donna Joseph, James A. |
| description | Previous studies show the enrichment of mammalian brain with neutral sphingomyelin-specific phospholipase C (ceramide-phosphocholine phosphodiesterase, EC 3.1.4.12; N-Sase), a key enzyme of sphingolipid metabolism and sphingolipid-induced signaling.
The objective of this study was to evaluate the membrane-associated and cytosolic N-Sase activities in the brain regions associated with behavior (striatum, hippocampus, and frontal cortex).
Results showed higher membrane-associated N-Sase activity as compared to the N-Sase activity in the cytosolic fractions of all the evaluated brain regions. In the hippocampus, the N-Sase activity was significantly higher than in the striatum and cortex. In addition, age-related changes in the hippocampal N-Sase activities were profoundly higher than in the respective fractions isolated from the striatum and cortex. Age-related decreases in the hippocampal and striatal cytosolic N-Sase activities were accompanied by increases in the membrane N-Sase activities in those brain regions. There was a significant increase in the cortical membrane-associated N-Sase activity with age; however, to a much lesser extend than in other brain regions. The increase in the hippocampal membrane-associated N-Sase activity was accompanied by a higher expression of the inflammatory marker, interleukin-1β (IL-1β), with age. One of the important findings of the present study is the region-specific expression of heat shock protein 70 (hsp70). Frontal cortex showed lower hsp70 expression in both young and old age groups as compared to the striatal and hippocampal hsp70 levels which can contribute to the recently reported higher cortical sensitivity to oxidative stress.
In conclusion (a) our results, for the first time to our knowledge, demonstrated the association between the N-Sase activity and the stress/inflammatory markers expression in the brain regions controlling behavior; (b) these findings suggest the role of N-Sase as a contributor to the increased stress and inflammatory sensitivity among the brain regions with age. |
| doi_str_mv | 10.1016/j.neuint.2005.06.011 |
| format | Article |
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The objective of this study was to evaluate the membrane-associated and cytosolic N-Sase activities in the brain regions associated with behavior (striatum, hippocampus, and frontal cortex).
Results showed higher membrane-associated N-Sase activity as compared to the N-Sase activity in the cytosolic fractions of all the evaluated brain regions. In the hippocampus, the N-Sase activity was significantly higher than in the striatum and cortex. In addition, age-related changes in the hippocampal N-Sase activities were profoundly higher than in the respective fractions isolated from the striatum and cortex. Age-related decreases in the hippocampal and striatal cytosolic N-Sase activities were accompanied by increases in the membrane N-Sase activities in those brain regions. There was a significant increase in the cortical membrane-associated N-Sase activity with age; however, to a much lesser extend than in other brain regions. The increase in the hippocampal membrane-associated N-Sase activity was accompanied by a higher expression of the inflammatory marker, interleukin-1β (IL-1β), with age. One of the important findings of the present study is the region-specific expression of heat shock protein 70 (hsp70). Frontal cortex showed lower hsp70 expression in both young and old age groups as compared to the striatal and hippocampal hsp70 levels which can contribute to the recently reported higher cortical sensitivity to oxidative stress.
In conclusion (a) our results, for the first time to our knowledge, demonstrated the association between the N-Sase activity and the stress/inflammatory markers expression in the brain regions controlling behavior; (b) these findings suggest the role of N-Sase as a contributor to the increased stress and inflammatory sensitivity among the brain regions with age.</description><identifier>ISSN: 0197-0186</identifier><identifier>EISSN: 1872-9754</identifier><identifier>DOI: 10.1016/j.neuint.2005.06.011</identifier><identifier>PMID: 16140422</identifier><identifier>CODEN: NEUIDS</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Aging - metabolism ; Animals ; Biological and medical sciences ; Biomarkers - metabolism ; Cell Membrane - enzymology ; Corpus Striatum - enzymology ; Corpus Striatum - physiopathology ; Cytokine interleukin-1β ; Disease Susceptibility - enzymology ; Disease Susceptibility - physiopathology ; Encephalitis - enzymology ; Encephalitis - physiopathology ; Fundamental and applied biological sciences. Psychology ; Heat shock protein 70 ; Hippocampus - enzymology ; Hippocampus - physiopathology ; HSP70 Heat-Shock Proteins - metabolism ; Inflammation Mediators - metabolism ; Interleukin-1 - metabolism ; Male ; Oxidative Stress - physiology ; Prefrontal Cortex - enzymology ; Prefrontal Cortex - physiopathology ; Rats ; Rats, Inbred F344 ; Sphingomyelin Phosphodiesterase - metabolism ; Sphingomyelins - metabolism ; Stress, Physiological - enzymology ; Stress, Physiological - physiopathology ; Subcellular fractions ; Telencephalon - anatomy & histology ; Telencephalon - enzymology ; Telencephalon - physiopathology ; Type C Phospholipases - metabolism ; Up-Regulation - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neurochemistry international, 2005-12, Vol.47 (8), p.573-579</ispartof><rights>2005 Elsevier Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-d00854cf68da210c9fc76bd3f68aac5a791e4c22035c63b6b462d05d0603d0e83</citedby><cites>FETCH-LOGICAL-c390t-d00854cf68da210c9fc76bd3f68aac5a791e4c22035c63b6b462d05d0603d0e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0197018605001919$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17186554$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16140422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Crivello, Natalia A.</creatorcontrib><creatorcontrib>Rosenberg, Irwin H.</creatorcontrib><creatorcontrib>Dallal, Gerard E.</creatorcontrib><creatorcontrib>Bielinski, Donna</creatorcontrib><creatorcontrib>Joseph, James A.</creatorcontrib><title>Age-related changes in neutral sphingomyelin-specific phospholipase C activity in striatum, hippocampus, and frontal cortex: Implication for sensitivity to stress and inflammation</title><title>Neurochemistry international</title><addtitle>Neurochem Int</addtitle><description>Previous studies show the enrichment of mammalian brain with neutral sphingomyelin-specific phospholipase C (ceramide-phosphocholine phosphodiesterase, EC 3.1.4.12; N-Sase), a key enzyme of sphingolipid metabolism and sphingolipid-induced signaling.
The objective of this study was to evaluate the membrane-associated and cytosolic N-Sase activities in the brain regions associated with behavior (striatum, hippocampus, and frontal cortex).
Results showed higher membrane-associated N-Sase activity as compared to the N-Sase activity in the cytosolic fractions of all the evaluated brain regions. In the hippocampus, the N-Sase activity was significantly higher than in the striatum and cortex. In addition, age-related changes in the hippocampal N-Sase activities were profoundly higher than in the respective fractions isolated from the striatum and cortex. Age-related decreases in the hippocampal and striatal cytosolic N-Sase activities were accompanied by increases in the membrane N-Sase activities in those brain regions. There was a significant increase in the cortical membrane-associated N-Sase activity with age; however, to a much lesser extend than in other brain regions. The increase in the hippocampal membrane-associated N-Sase activity was accompanied by a higher expression of the inflammatory marker, interleukin-1β (IL-1β), with age. One of the important findings of the present study is the region-specific expression of heat shock protein 70 (hsp70). Frontal cortex showed lower hsp70 expression in both young and old age groups as compared to the striatal and hippocampal hsp70 levels which can contribute to the recently reported higher cortical sensitivity to oxidative stress.
In conclusion (a) our results, for the first time to our knowledge, demonstrated the association between the N-Sase activity and the stress/inflammatory markers expression in the brain regions controlling behavior; (b) these findings suggest the role of N-Sase as a contributor to the increased stress and inflammatory sensitivity among the brain regions with age.</description><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Cell Membrane - enzymology</subject><subject>Corpus Striatum - enzymology</subject><subject>Corpus Striatum - physiopathology</subject><subject>Cytokine interleukin-1β</subject><subject>Disease Susceptibility - enzymology</subject><subject>Disease Susceptibility - physiopathology</subject><subject>Encephalitis - enzymology</subject><subject>Encephalitis - physiopathology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heat shock protein 70</subject><subject>Hippocampus - enzymology</subject><subject>Hippocampus - physiopathology</subject><subject>HSP70 Heat-Shock Proteins - metabolism</subject><subject>Inflammation Mediators - metabolism</subject><subject>Interleukin-1 - metabolism</subject><subject>Male</subject><subject>Oxidative Stress - physiology</subject><subject>Prefrontal Cortex - enzymology</subject><subject>Prefrontal Cortex - physiopathology</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Sphingomyelin Phosphodiesterase - metabolism</subject><subject>Sphingomyelins - metabolism</subject><subject>Stress, Physiological - enzymology</subject><subject>Stress, Physiological - physiopathology</subject><subject>Subcellular fractions</subject><subject>Telencephalon - anatomy & histology</subject><subject>Telencephalon - enzymology</subject><subject>Telencephalon - physiopathology</subject><subject>Type C Phospholipases - metabolism</subject><subject>Up-Regulation - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0197-0186</issn><issn>1872-9754</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU-L1DAYh4so7rj6DURy0dO2vumftPUgLIN_Fha86Dlkkrcz79AmNUkX53P5Bc3sFPbmqZA-v4eQJ8vecig4cPHxWFhcyMaiBGgKEAVw_izb8K4t875t6ufZBnjf5sA7cZW9CuEIAG0PzcvsigteQ12Wm-zv7R5zj6OKaJg-KLvHwMiy5I5ejSzMB7J7N51wJJuHGTUNpNl8cOmPG2lWAdmWKR3pgeLpPA3Rk4rLdMMONM9Oq2lewg1T1rDBOxuTVTsf8c8ndjfNI2kVyVk2OM8C2kCrKbqzCUN4XJIdRjVNj-jr7MWgxoBv1u919uvrl5_b7_n9j29329v7XFc9xNwAdE2tB9EZVXLQ_aBbsTNVOlBKN6rtOda6LKFqtKh2YleL0kBjQEBlALvqOvtw8c7e_V4wRDlR0DiOyqJbghSd6HvRlQmsL6D2LgSPg5w9TcqfJAd5jiWP8hJLnmNJEDLFSrN3q3_ZTWieRmudBLxfARW0GgevrKbwxLUpbdPUift84TC9xgOhl0ETWo2GPOoojaP_3-Qff2a6Bw</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Crivello, Natalia A.</creator><creator>Rosenberg, Irwin H.</creator><creator>Dallal, Gerard E.</creator><creator>Bielinski, Donna</creator><creator>Joseph, James A.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>Age-related changes in neutral sphingomyelin-specific phospholipase C activity in striatum, hippocampus, and frontal cortex: Implication for sensitivity to stress and inflammation</title><author>Crivello, Natalia A. ; Rosenberg, Irwin H. ; Dallal, Gerard E. ; Bielinski, Donna ; Joseph, James A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-d00854cf68da210c9fc76bd3f68aac5a791e4c22035c63b6b462d05d0603d0e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Cell Membrane - enzymology</topic><topic>Corpus Striatum - enzymology</topic><topic>Corpus Striatum - physiopathology</topic><topic>Cytokine interleukin-1β</topic><topic>Disease Susceptibility - enzymology</topic><topic>Disease Susceptibility - physiopathology</topic><topic>Encephalitis - enzymology</topic><topic>Encephalitis - physiopathology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heat shock protein 70</topic><topic>Hippocampus - enzymology</topic><topic>Hippocampus - physiopathology</topic><topic>HSP70 Heat-Shock Proteins - metabolism</topic><topic>Inflammation Mediators - metabolism</topic><topic>Interleukin-1 - metabolism</topic><topic>Male</topic><topic>Oxidative Stress - physiology</topic><topic>Prefrontal Cortex - enzymology</topic><topic>Prefrontal Cortex - physiopathology</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Sphingomyelin Phosphodiesterase - metabolism</topic><topic>Sphingomyelins - metabolism</topic><topic>Stress, Physiological - enzymology</topic><topic>Stress, Physiological - physiopathology</topic><topic>Subcellular fractions</topic><topic>Telencephalon - anatomy & histology</topic><topic>Telencephalon - enzymology</topic><topic>Telencephalon - physiopathology</topic><topic>Type C Phospholipases - metabolism</topic><topic>Up-Regulation - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Crivello, Natalia A.</creatorcontrib><creatorcontrib>Rosenberg, Irwin H.</creatorcontrib><creatorcontrib>Dallal, Gerard E.</creatorcontrib><creatorcontrib>Bielinski, Donna</creatorcontrib><creatorcontrib>Joseph, James A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemistry international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Crivello, Natalia A.</au><au>Rosenberg, Irwin H.</au><au>Dallal, Gerard E.</au><au>Bielinski, Donna</au><au>Joseph, James A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-related changes in neutral sphingomyelin-specific phospholipase C activity in striatum, hippocampus, and frontal cortex: Implication for sensitivity to stress and inflammation</atitle><jtitle>Neurochemistry international</jtitle><addtitle>Neurochem Int</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>47</volume><issue>8</issue><spage>573</spage><epage>579</epage><pages>573-579</pages><issn>0197-0186</issn><eissn>1872-9754</eissn><coden>NEUIDS</coden><abstract>Previous studies show the enrichment of mammalian brain with neutral sphingomyelin-specific phospholipase C (ceramide-phosphocholine phosphodiesterase, EC 3.1.4.12; N-Sase), a key enzyme of sphingolipid metabolism and sphingolipid-induced signaling.
The objective of this study was to evaluate the membrane-associated and cytosolic N-Sase activities in the brain regions associated with behavior (striatum, hippocampus, and frontal cortex).
Results showed higher membrane-associated N-Sase activity as compared to the N-Sase activity in the cytosolic fractions of all the evaluated brain regions. In the hippocampus, the N-Sase activity was significantly higher than in the striatum and cortex. In addition, age-related changes in the hippocampal N-Sase activities were profoundly higher than in the respective fractions isolated from the striatum and cortex. Age-related decreases in the hippocampal and striatal cytosolic N-Sase activities were accompanied by increases in the membrane N-Sase activities in those brain regions. There was a significant increase in the cortical membrane-associated N-Sase activity with age; however, to a much lesser extend than in other brain regions. The increase in the hippocampal membrane-associated N-Sase activity was accompanied by a higher expression of the inflammatory marker, interleukin-1β (IL-1β), with age. One of the important findings of the present study is the region-specific expression of heat shock protein 70 (hsp70). Frontal cortex showed lower hsp70 expression in both young and old age groups as compared to the striatal and hippocampal hsp70 levels which can contribute to the recently reported higher cortical sensitivity to oxidative stress.
In conclusion (a) our results, for the first time to our knowledge, demonstrated the association between the N-Sase activity and the stress/inflammatory markers expression in the brain regions controlling behavior; (b) these findings suggest the role of N-Sase as a contributor to the increased stress and inflammatory sensitivity among the brain regions with age.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16140422</pmid><doi>10.1016/j.neuint.2005.06.011</doi><tpages>7</tpages></addata></record> |
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| ispartof | Neurochemistry international, 2005-12, Vol.47 (8), p.573-579 |
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| subjects | Aging - metabolism Animals Biological and medical sciences Biomarkers - metabolism Cell Membrane - enzymology Corpus Striatum - enzymology Corpus Striatum - physiopathology Cytokine interleukin-1β Disease Susceptibility - enzymology Disease Susceptibility - physiopathology Encephalitis - enzymology Encephalitis - physiopathology Fundamental and applied biological sciences. Psychology Heat shock protein 70 Hippocampus - enzymology Hippocampus - physiopathology HSP70 Heat-Shock Proteins - metabolism Inflammation Mediators - metabolism Interleukin-1 - metabolism Male Oxidative Stress - physiology Prefrontal Cortex - enzymology Prefrontal Cortex - physiopathology Rats Rats, Inbred F344 Sphingomyelin Phosphodiesterase - metabolism Sphingomyelins - metabolism Stress, Physiological - enzymology Stress, Physiological - physiopathology Subcellular fractions Telencephalon - anatomy & histology Telencephalon - enzymology Telencephalon - physiopathology Type C Phospholipases - metabolism Up-Regulation - physiology Vertebrates: nervous system and sense organs |
| title | Age-related changes in neutral sphingomyelin-specific phospholipase C activity in striatum, hippocampus, and frontal cortex: Implication for sensitivity to stress and inflammation |
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