Multiplexed Detection of Antibodies to Nonstructural Proteins of Foot-and-Mouth Disease Virus
Liquid array technology was used to develop a multiplexed assay for the detection of antibodies to viral nonstructural proteins (NSPs), raised in cattle in response to infection with foot-and-mouth disease (FMD) virus. Two assays, one based on recombinant NSPs and the other on synthetically produced...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2006-08, Vol.78 (15), p.5462-5468 |
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| creator | Perkins, Julie Clavijo, Alfonso Hindson, Benjamin J Lenhoff, Raymond J McBride, Mary T |
| description | Liquid array technology was used to develop a multiplexed assay for the detection of antibodies to viral nonstructural proteins (NSPs), raised in cattle in response to infection with foot-and-mouth disease (FMD) virus. Two assays, one based on recombinant NSPs and the other on synthetically produced peptides, were developed and compared side-by-side. Serum samples from serial bleeds of cattle, each experimentally infected with one of the seven serotypes (C, A, O, Asia, SAT1, SAT2, SAT3) of FMD virus were analyzed. A distinct pattern in the detection of NSP antibodies and a close correlation of the recombinant protein and peptide-based assays were observed. The detection of antibodies to NSPs is a method to differentiate FMD-infected and FMD-vaccinated animals, and a high-throughput assay would be an invaluable tool in the case of an outbreak of FMD in North America, when emergency vaccination may be utilized to spare vaccinated, noninfected animals from slaughter and subsequent disposal. |
| doi_str_mv | 10.1021/ac060235e |
| format | Article |
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Two assays, one based on recombinant NSPs and the other on synthetically produced peptides, were developed and compared side-by-side. Serum samples from serial bleeds of cattle, each experimentally infected with one of the seven serotypes (C, A, O, Asia, SAT1, SAT2, SAT3) of FMD virus were analyzed. A distinct pattern in the detection of NSP antibodies and a close correlation of the recombinant protein and peptide-based assays were observed. The detection of antibodies to NSPs is a method to differentiate FMD-infected and FMD-vaccinated animals, and a high-throughput assay would be an invaluable tool in the case of an outbreak of FMD in North America, when emergency vaccination may be utilized to spare vaccinated, noninfected animals from slaughter and subsequent disposal.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac060235e</identifier><identifier>PMID: 16878883</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Animals ; Antibodies, Viral - blood ; Biochemistry ; Biological and medical sciences ; Cattle ; Enzyme-Linked Immunosorbent Assay - methods ; Foot-and-Mouth Disease - blood ; Foot-and-Mouth Disease - diagnosis ; Foot-and-mouth disease virus ; Foot-and-Mouth Disease Virus - immunology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Microspheres ; Molecular immunology ; Proteins ; Scientific apparatus & instruments ; Sensitivity and Specificity ; Techniques ; Viral Proteins - immunology ; Viruses</subject><ispartof>Analytical chemistry (Washington), 2006-08, Vol.78 (15), p.5462-5468</ispartof><rights>Copyright © 2006 American Chemical Society</rights><rights>2006 INIST-CNRS</rights><rights>Copyright American Chemical Society Aug 1, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a439t-c402300be3e6b0023ada31c6b14c6d1de136e8947c096814a0d65b21440196ba3</citedby><cites>FETCH-LOGICAL-a439t-c402300be3e6b0023ada31c6b14c6d1de136e8947c096814a0d65b21440196ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac060235e$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac060235e$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18013132$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16878883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Perkins, Julie</creatorcontrib><creatorcontrib>Clavijo, Alfonso</creatorcontrib><creatorcontrib>Hindson, Benjamin J</creatorcontrib><creatorcontrib>Lenhoff, Raymond J</creatorcontrib><creatorcontrib>McBride, Mary T</creatorcontrib><title>Multiplexed Detection of Antibodies to Nonstructural Proteins of Foot-and-Mouth Disease Virus</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Liquid array technology was used to develop a multiplexed assay for the detection of antibodies to viral nonstructural proteins (NSPs), raised in cattle in response to infection with foot-and-mouth disease (FMD) virus. Two assays, one based on recombinant NSPs and the other on synthetically produced peptides, were developed and compared side-by-side. Serum samples from serial bleeds of cattle, each experimentally infected with one of the seven serotypes (C, A, O, Asia, SAT1, SAT2, SAT3) of FMD virus were analyzed. A distinct pattern in the detection of NSP antibodies and a close correlation of the recombinant protein and peptide-based assays were observed. The detection of antibodies to NSPs is a method to differentiate FMD-infected and FMD-vaccinated animals, and a high-throughput assay would be an invaluable tool in the case of an outbreak of FMD in North America, when emergency vaccination may be utilized to spare vaccinated, noninfected animals from slaughter and subsequent disposal.</description><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Foot-and-Mouth Disease - blood</subject><subject>Foot-and-Mouth Disease - diagnosis</subject><subject>Foot-and-mouth disease virus</subject><subject>Foot-and-Mouth Disease Virus - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Microspheres</subject><subject>Molecular immunology</subject><subject>Proteins</subject><subject>Scientific apparatus & instruments</subject><subject>Sensitivity and Specificity</subject><subject>Techniques</subject><subject>Viral Proteins - immunology</subject><subject>Viruses</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0V1rFDEUBuAgSrvWXvgHZBAUvBg9J5nJZC5La6vYbQtdvZOQyZzF1NnJNh_Q_ntn2aULeuFVDuTh5Rxexl4jfETg-MlYkMBFTc_YDGsOpVSKP2czABAlbwAO2csY7wAQAeUBO0SpGqWUmLGf8zwktx7ogfrijBLZ5PxY-GVxMibX-d5RLJIvrvwYU8g25WCG4ib4RG6MG3fufSrN2Jdzn9Ov4sxFMpGKHy7k-Iq9WJoh0vHuPWLfzz8vTr-Ul9cXX09PLktTiTaVtpq2B-hIkOxgmk1vBFrZYWVljz2hkKTaqrHQSoWVgV7WHceqAmxlZ8QRe7_NXQd_nykmvXLR0jCYkXyOWirZ1hzr_0KOULWNFBN8-xe88zmM0xGTaVTDebVBH7bIBh9joKVeB7cy4VEj6E0z-qmZyb7ZBeZuRf1e7qqYwLsdMNGaYRnMaF3cOwUoUPDJlVvnYqKHp38TfmvZiKbWi5tbffUN1aKFhZ7vc42N-yP-XfAPPaiu8g</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Perkins, Julie</creator><creator>Clavijo, Alfonso</creator><creator>Hindson, Benjamin J</creator><creator>Lenhoff, Raymond J</creator><creator>McBride, Mary T</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7T7</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>Multiplexed Detection of Antibodies to Nonstructural Proteins of Foot-and-Mouth Disease Virus</title><author>Perkins, Julie ; Clavijo, Alfonso ; Hindson, Benjamin J ; Lenhoff, Raymond J ; McBride, Mary T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a439t-c402300be3e6b0023ada31c6b14c6d1de136e8947c096814a0d65b21440196ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Foot-and-Mouth Disease - blood</topic><topic>Foot-and-Mouth Disease - diagnosis</topic><topic>Foot-and-mouth disease virus</topic><topic>Foot-and-Mouth Disease Virus - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Microspheres</topic><topic>Molecular immunology</topic><topic>Proteins</topic><topic>Scientific apparatus & instruments</topic><topic>Sensitivity and Specificity</topic><topic>Techniques</topic><topic>Viral Proteins - immunology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Perkins, Julie</creatorcontrib><creatorcontrib>Clavijo, Alfonso</creatorcontrib><creatorcontrib>Hindson, Benjamin J</creatorcontrib><creatorcontrib>Lenhoff, Raymond J</creatorcontrib><creatorcontrib>McBride, Mary T</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Perkins, Julie</au><au>Clavijo, Alfonso</au><au>Hindson, Benjamin J</au><au>Lenhoff, Raymond J</au><au>McBride, Mary T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiplexed Detection of Antibodies to Nonstructural Proteins of Foot-and-Mouth Disease Virus</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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| subjects | Analytical chemistry Animals Antibodies, Viral - blood Biochemistry Biological and medical sciences Cattle Enzyme-Linked Immunosorbent Assay - methods Foot-and-Mouth Disease - blood Foot-and-Mouth Disease - diagnosis Foot-and-mouth disease virus Foot-and-Mouth Disease Virus - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology Microspheres Molecular immunology Proteins Scientific apparatus & instruments Sensitivity and Specificity Techniques Viral Proteins - immunology Viruses |
| title | Multiplexed Detection of Antibodies to Nonstructural Proteins of Foot-and-Mouth Disease Virus |
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