Simultaneous determination of adenine and guanine in ruminant bacterial pellets by ion-pair HPLC
An ion-pair reversed-phase high-performance liquid chromatography with gradient elution and UV detection was used to measure adenine (A) and guanine (G) in lyophilized bacterial pellets from ruminants using allopurinol as internal standard. The separation was performed on a Symmetry C18 column and t...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-11, Vol.826 (1), p.257-260 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | del Moral, Pilar García Arín, María Jesús Resines, José Antonio Díez, María Teresa |
| description | An ion-pair reversed-phase high-performance liquid chromatography with gradient elution and UV detection was used to measure adenine (A) and guanine (G) in lyophilized bacterial pellets from ruminants using allopurinol as internal standard. The separation was performed on a Symmetry C18 column and the detection was monitored at 280
nm. Calibration curves were found to be linear in the concentration range from 5 to 50
mg/l with correlation coefficients (
r
2)
>
0.999. Mean recoveries of A and G standards added to bacterial samples were 102.2 and 98.2, respectively. The method proposed yielded sharp, well-resolved peaks within 25
min and was successfully applied for the determination of A and G in bacterial pellets. |
| doi_str_mv | 10.1016/j.jchromb.2005.07.044 |
| format | Article |
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nm. Calibration curves were found to be linear in the concentration range from 5 to 50
mg/l with correlation coefficients (
r
2)
>
0.999. Mean recoveries of A and G standards added to bacterial samples were 102.2 and 98.2, respectively. The method proposed yielded sharp, well-resolved peaks within 25
min and was successfully applied for the determination of A and G in bacterial pellets.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2005.07.044</identifier><identifier>PMID: 16122994</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adenine - analysis ; Analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Bacteria - chemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Guanine - analysis ; Ion-pair reversed-phase high-performance liquid chromatography ; Medical sciences ; Pharmacology. Drug treatments ; Purine bases: Adenine and Guanine ; Rumen - microbiology</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005-11, Vol.826 (1), p.257-260</ispartof><rights>2005 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-9a4f7701fb4760365da4dd5bcfbf9b5803a0ee694b4140c0edc1b68b34f366e93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1570023205005507$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17196391$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16122994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>del Moral, Pilar García</creatorcontrib><creatorcontrib>Arín, María Jesús</creatorcontrib><creatorcontrib>Resines, José Antonio</creatorcontrib><creatorcontrib>Díez, María Teresa</creatorcontrib><title>Simultaneous determination of adenine and guanine in ruminant bacterial pellets by ion-pair HPLC</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>An ion-pair reversed-phase high-performance liquid chromatography with gradient elution and UV detection was used to measure adenine (A) and guanine (G) in lyophilized bacterial pellets from ruminants using allopurinol as internal standard. The separation was performed on a Symmetry C18 column and the detection was monitored at 280
nm. Calibration curves were found to be linear in the concentration range from 5 to 50
mg/l with correlation coefficients (
r
2)
>
0.999. Mean recoveries of A and G standards added to bacterial samples were 102.2 and 98.2, respectively. The method proposed yielded sharp, well-resolved peaks within 25
min and was successfully applied for the determination of A and G in bacterial pellets.</description><subject>Adenine - analysis</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Bacteria - chemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Guanine - analysis</subject><subject>Ion-pair reversed-phase high-performance liquid chromatography</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Purine bases: Adenine and Guanine</subject><subject>Rumen - microbiology</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo7rr6E5Rc9NZtpZNOJieRQV1hQEEFbzEf1ZqhOz0m3cL-ezNOwx49VR2eN_XmIeQ5g5YBk6-P7dH_yvPk2g6gb0G1IMQDcs12ijdcye8P694raKDj3RV5UsoRgClQ_DG5YpJ1ndbimvz4Eqd1XGzCeS004IJ5iskucU50HqgNmGJCalOgP1f7b4-J5vUMpYU662si2pGecBxxKdTd0ZptTjZmevv5sH9KHg12LPhsmzfk2_t3X_e3zeHTh4_7t4fGi65bGm3FoBSwwQklgcs-WBFC7_zgBu36HXALiFILJ5gADxg8c3LnuBi4lKj5DXl1efeU598rlsVMsfha6vI1I3dS99VABfsL6PNcSsbBnHKcbL4zDMxZrTmaTa05qzWgTFVbcy-2A6ubMNynNpcVeLkBtng7DtkmH8s9p5iWXJ8LvLlwWHX8iZhN8RGTxxAz-sWEOf6nyl_wUJsV</recordid><startdate>20051105</startdate><enddate>20051105</enddate><creator>del Moral, Pilar García</creator><creator>Arín, María Jesús</creator><creator>Resines, José Antonio</creator><creator>Díez, María Teresa</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051105</creationdate><title>Simultaneous determination of adenine and guanine in ruminant bacterial pellets by ion-pair HPLC</title><author>del Moral, Pilar García ; Arín, María Jesús ; Resines, José Antonio ; Díez, María Teresa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-9a4f7701fb4760365da4dd5bcfbf9b5803a0ee694b4140c0edc1b68b34f366e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenine - analysis</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Bacteria - chemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Guanine - analysis</topic><topic>Ion-pair reversed-phase high-performance liquid chromatography</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Purine bases: Adenine and Guanine</topic><topic>Rumen - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>del Moral, Pilar García</creatorcontrib><creatorcontrib>Arín, María Jesús</creatorcontrib><creatorcontrib>Resines, José Antonio</creatorcontrib><creatorcontrib>Díez, María Teresa</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>del Moral, Pilar García</au><au>Arín, María Jesús</au><au>Resines, José Antonio</au><au>Díez, María Teresa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of adenine and guanine in ruminant bacterial pellets by ion-pair HPLC</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2005-11-05</date><risdate>2005</risdate><volume>826</volume><issue>1</issue><spage>257</spage><epage>260</epage><pages>257-260</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>An ion-pair reversed-phase high-performance liquid chromatography with gradient elution and UV detection was used to measure adenine (A) and guanine (G) in lyophilized bacterial pellets from ruminants using allopurinol as internal standard. The separation was performed on a Symmetry C18 column and the detection was monitored at 280
nm. Calibration curves were found to be linear in the concentration range from 5 to 50
mg/l with correlation coefficients (
r
2)
>
0.999. Mean recoveries of A and G standards added to bacterial samples were 102.2 and 98.2, respectively. The method proposed yielded sharp, well-resolved peaks within 25
min and was successfully applied for the determination of A and G in bacterial pellets.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16122994</pmid><doi>10.1016/j.jchromb.2005.07.044</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005-11, Vol.826 (1), p.257-260 |
| issn | 1570-0232 1873-376X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenine - analysis Analysis Analytical, structural and metabolic biochemistry Animals Bacteria - chemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Fundamental and applied biological sciences. Psychology General pharmacology Guanine - analysis Ion-pair reversed-phase high-performance liquid chromatography Medical sciences Pharmacology. Drug treatments Purine bases: Adenine and Guanine Rumen - microbiology |
| title | Simultaneous determination of adenine and guanine in ruminant bacterial pellets by ion-pair HPLC |
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