Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana
We investigated the fourth subgroup of Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in Arabidopsis cells and...
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| Veröffentlicht in: | FEBS letters 2005-10, Vol.579 (25), p.5814-5820 |
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| creator | Ishikawa, Fumiyoshi Suga, Shinobu Uemura, Tomohiro Sato, Masa H. Maeshima, Masayoshi |
| description | We investigated the fourth subgroup of
Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in
Arabidopsis cells and the subcellular fractionation of the tissue homogenate showed their ER localization. The SIP proteins were detected in all of the tissues, except for dry seeds. Histochemical analysis of promoter-β-glucuronidase fusions revealed the cell-specific expression of SIPs. SIP1;1 and SIP1;2 may function as water channels in the ER, while SIP2;1 might act as an ER channel for other small molecules or ions. |
| doi_str_mv | 10.1016/j.febslet.2005.09.076 |
| format | Article |
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Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in
Arabidopsis cells and the subcellular fractionation of the tissue homogenate showed their ER localization. The SIP proteins were detected in all of the tissues, except for dry seeds. Histochemical analysis of promoter-β-glucuronidase fusions revealed the cell-specific expression of SIPs. SIP1;1 and SIP1;2 may function as water channels in the ER, while SIP2;1 might act as an ER channel for other small molecules or ions.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2005.09.076</identifier><identifier>PMID: 16223486</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>AHA3 ; Aquaporin ; Aquaporins - analysis ; Aquaporins - genetics ; Aquaporins - metabolism ; Arabidopsis ; Arabidopsis - metabolism ; Arabidopsis plasma-membrane H+-ATPase 3 ; Arabidopsis Proteins - analysis ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; binding protein ; BiP ; Endoplasmic Reticulum - chemistry ; Endoplasmic Reticulum - metabolism ; Gene Expression ; GFP ; green fluorescent protein ; Green Fluorescent Proteins - analysis ; Green Fluorescent Proteins - genetics ; GUS ; Intracellular Membranes - chemistry ; Intracellular Membranes - metabolism ; NIP ; Nod26-like intrinsic proteins ; PIP ; plasma-membrane intrinsic protein ; Promoter Regions, Genetic ; Saccharomyces cerevisiae - genetics ; SIP ; Small and basic intrinsic protein ; TIP ; Tissue Distribution ; tonoplast intrinsic protein ; vacuolar H+-ATPase ; VHA ; Water channel ; β-glucuronidase</subject><ispartof>FEBS letters, 2005-10, Vol.579 (25), p.5814-5820</ispartof><rights>2005</rights><rights>FEBS Letters 579 (2005) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5416-937d83abd98427d8729d262e77950e9d50bf7bb4d94ba5f45e34c51cb8374c063</citedby><cites>FETCH-LOGICAL-c5416-937d83abd98427d8729d262e77950e9d50bf7bb4d94ba5f45e34c51cb8374c063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2005.09.076$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579305011956$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16223486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ishikawa, Fumiyoshi</creatorcontrib><creatorcontrib>Suga, Shinobu</creatorcontrib><creatorcontrib>Uemura, Tomohiro</creatorcontrib><creatorcontrib>Sato, Masa H.</creatorcontrib><creatorcontrib>Maeshima, Masayoshi</creatorcontrib><title>Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We investigated the fourth subgroup of
Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in
Arabidopsis cells and the subcellular fractionation of the tissue homogenate showed their ER localization. The SIP proteins were detected in all of the tissues, except for dry seeds. Histochemical analysis of promoter-β-glucuronidase fusions revealed the cell-specific expression of SIPs. SIP1;1 and SIP1;2 may function as water channels in the ER, while SIP2;1 might act as an ER channel for other small molecules or ions.</description><subject>AHA3</subject><subject>Aquaporin</subject><subject>Aquaporins - analysis</subject><subject>Aquaporins - genetics</subject><subject>Aquaporins - metabolism</subject><subject>Arabidopsis</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis plasma-membrane H+-ATPase 3</subject><subject>Arabidopsis Proteins - analysis</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>binding protein</subject><subject>BiP</subject><subject>Endoplasmic Reticulum - chemistry</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Gene Expression</subject><subject>GFP</subject><subject>green fluorescent protein</subject><subject>Green Fluorescent Proteins - analysis</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>GUS</subject><subject>Intracellular Membranes - chemistry</subject><subject>Intracellular Membranes - metabolism</subject><subject>NIP</subject><subject>Nod26-like intrinsic proteins</subject><subject>PIP</subject><subject>plasma-membrane intrinsic protein</subject><subject>Promoter Regions, Genetic</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>SIP</subject><subject>Small and basic intrinsic protein</subject><subject>TIP</subject><subject>Tissue Distribution</subject><subject>tonoplast intrinsic protein</subject><subject>vacuolar H+-ATPase</subject><subject>VHA</subject><subject>Water channel</subject><subject>β-glucuronidase</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhEUBescvUjn8Sr1CpprRSBYjC2vLPjepREqd2pu3Ay-NoRuoSVralcz8ffReh95SsKaHybLvuwOYe5nVNiFgTtSaNfIFWtG1YxbhsX6IVIZRXolHsBL3JeUvKu6XqNTqhsq4Zb-UK_fkaH6DH834CbO53ZoopjPj2-nvGJgEeTBj7Pe6jM334DR7PEc93gDc_8ACDTWYsY6PH-S4-Ygd9X-UJXOiCw_A0Jcg5xBEX4nkyNvg45ZALoMDMaN6iV53pM7w7nqfo1-Xm58VVdfPty_XF-U3lBKeyUqzxLTPWq5bX5drUyteyhqZRgoDygtiusZZ7xa0RHRfAuBPU2ZY13BHJTtHHA3dK8X4HedZDyEvZ0j7uspatVEwyVYLiEHQp5pyg01MKg0l7TYlerOutPlrXi3VNlC7Wy9yH4wc7O4B_njpqLoGrQ-Ax9LD_P6q-3Hyub5cVLhskglCqxIL6dEBBMfYQIOnsAowOfEjgZu1j-Efbv_fzrL0</recordid><startdate>20051024</startdate><enddate>20051024</enddate><creator>Ishikawa, Fumiyoshi</creator><creator>Suga, Shinobu</creator><creator>Uemura, Tomohiro</creator><creator>Sato, Masa H.</creator><creator>Maeshima, Masayoshi</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051024</creationdate><title>Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana</title><author>Ishikawa, Fumiyoshi ; Suga, Shinobu ; Uemura, Tomohiro ; Sato, Masa H. ; Maeshima, Masayoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5416-937d83abd98427d8729d262e77950e9d50bf7bb4d94ba5f45e34c51cb8374c063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>AHA3</topic><topic>Aquaporin</topic><topic>Aquaporins - analysis</topic><topic>Aquaporins - genetics</topic><topic>Aquaporins - metabolism</topic><topic>Arabidopsis</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis plasma-membrane H+-ATPase 3</topic><topic>Arabidopsis Proteins - analysis</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>binding protein</topic><topic>BiP</topic><topic>Endoplasmic Reticulum - chemistry</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Gene Expression</topic><topic>GFP</topic><topic>green fluorescent protein</topic><topic>Green Fluorescent Proteins - analysis</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>GUS</topic><topic>Intracellular Membranes - chemistry</topic><topic>Intracellular Membranes - metabolism</topic><topic>NIP</topic><topic>Nod26-like intrinsic proteins</topic><topic>PIP</topic><topic>plasma-membrane intrinsic protein</topic><topic>Promoter Regions, Genetic</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>SIP</topic><topic>Small and basic intrinsic protein</topic><topic>TIP</topic><topic>Tissue Distribution</topic><topic>tonoplast intrinsic protein</topic><topic>vacuolar H+-ATPase</topic><topic>VHA</topic><topic>Water channel</topic><topic>β-glucuronidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishikawa, Fumiyoshi</creatorcontrib><creatorcontrib>Suga, Shinobu</creatorcontrib><creatorcontrib>Uemura, Tomohiro</creatorcontrib><creatorcontrib>Sato, Masa H.</creatorcontrib><creatorcontrib>Maeshima, Masayoshi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishikawa, Fumiyoshi</au><au>Suga, Shinobu</au><au>Uemura, Tomohiro</au><au>Sato, Masa H.</au><au>Maeshima, Masayoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2005-10-24</date><risdate>2005</risdate><volume>579</volume><issue>25</issue><spage>5814</spage><epage>5820</epage><pages>5814-5820</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We investigated the fourth subgroup of
Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in
Arabidopsis cells and the subcellular fractionation of the tissue homogenate showed their ER localization. The SIP proteins were detected in all of the tissues, except for dry seeds. Histochemical analysis of promoter-β-glucuronidase fusions revealed the cell-specific expression of SIPs. SIP1;1 and SIP1;2 may function as water channels in the ER, while SIP2;1 might act as an ER channel for other small molecules or ions.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>16223486</pmid><doi>10.1016/j.febslet.2005.09.076</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | AHA3 Aquaporin Aquaporins - analysis Aquaporins - genetics Aquaporins - metabolism Arabidopsis Arabidopsis - metabolism Arabidopsis plasma-membrane H+-ATPase 3 Arabidopsis Proteins - analysis Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism binding protein BiP Endoplasmic Reticulum - chemistry Endoplasmic Reticulum - metabolism Gene Expression GFP green fluorescent protein Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics GUS Intracellular Membranes - chemistry Intracellular Membranes - metabolism NIP Nod26-like intrinsic proteins PIP plasma-membrane intrinsic protein Promoter Regions, Genetic Saccharomyces cerevisiae - genetics SIP Small and basic intrinsic protein TIP Tissue Distribution tonoplast intrinsic protein vacuolar H+-ATPase VHA Water channel β-glucuronidase |
| title | Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana |
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