Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay
A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportio...
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Veröffentlicht in: | Diagnostic microbiology and infectious disease 2005-10, Vol.53 (2), p.121-124 |
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container_title | Diagnostic microbiology and infectious disease |
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creator | Kumar, Manoj Khan, Inshad Ali Verma, Vijashoor Kalyan, Narender Qazi, Giulam Nabi |
description | A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of
Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for
M. tuberculosis makes it more suitable method for MIC determination. |
doi_str_mv | 10.1016/j.diagmicrobio.2005.05.011 |
format | Article |
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Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for
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Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for
M. tuberculosis makes it more suitable method for MIC determination.</description><subject>ABA</subject><subject>Agar proportion method</subject><subject>Antitubercular Agents - pharmacology</subject><subject>Antitubercular Agents - therapeutic use</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>MIC</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - enzymology</subject><subject>Mycobacterium tuberculosis - metabolism</subject><subject>Nitrate Reductase - analysis</subject><subject>Nitrate Reductase - metabolism</subject><subject>Nitrates - metabolism</subject><subject>NRA</subject><subject>Reagent Kits, Diagnostic</subject><subject>Reproducibility of Results</subject><subject>Tuberculosis - drug therapy</subject><subject>Tuberculosis - microbiology</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkVuL1TAQx4O4uMfVryBB0Cd7NuklTXyT421hF0H0OaTJdJlD29RMu3q-_baeA-ubCzMMDL-5_hl7LcVWCqku99uA7rZHn2KDcZsLUW1Xk_IJ20hdm0yIWjxlG1EXeaa1Kc7Zc6K9EDI3pXjGzqWSSi--Yb-_uxHDO44D_BlhILwDfnO14wEmSD0ObsI48Njym4OPjfNLFueeT3MDyc9dJCSOFDs3AfHmwGfC4Zb_3W1ck3zAKa0xQZj95Ai4I3KHF-ysdR3By1O8YD8_f_qx-5pdf_tytftwnfmyMFPmlNZlFaTJ69zltXQOalGCKrQvRGO0VsJXJrSh9No0ptROyUZD4b0QpixkccHeHvuOKf6agSbbI3noOjdAnMkub1ia1P8HpakrJUuxgO-P4HIiUYLWjgl7lw5WCrvqY_f2X33sqo9dTa5TXp2mzE0P4aH0JMgCvDkBjrzr2uQGj_TA1XlhlKoW7uORg-V5dwjJkkcYPARM4CcbIj5mn3szprcc</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Kumar, Manoj</creator><creator>Khan, Inshad Ali</creator><creator>Verma, Vijashoor</creator><creator>Kalyan, Narender</creator><creator>Qazi, Giulam Nabi</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20051001</creationdate><title>Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay</title><author>Kumar, Manoj ; Khan, Inshad Ali ; Verma, Vijashoor ; Kalyan, Narender ; Qazi, Giulam Nabi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-a68845d19272a271aae704e638c30b98860c59dfd4c89b948a61b8e3cc0094313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>ABA</topic><topic>Agar proportion method</topic><topic>Antitubercular Agents - pharmacology</topic><topic>Antitubercular Agents - therapeutic use</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - methods</topic><topic>Fundamental and applied biological sciences. 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Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for
M. tuberculosis makes it more suitable method for MIC determination.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16168616</pmid><doi>10.1016/j.diagmicrobio.2005.05.011</doi><tpages>4</tpages></addata></record> |
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subjects | ABA Agar proportion method Antitubercular Agents - pharmacology Antitubercular Agents - therapeutic use Bacterial diseases Biological and medical sciences Biological Assay - methods Fundamental and applied biological sciences. Psychology Human bacterial diseases Humans Infectious diseases Medical sciences MIC Microbial Sensitivity Tests Microbiology Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - enzymology Mycobacterium tuberculosis - metabolism Nitrate Reductase - analysis Nitrate Reductase - metabolism Nitrates - metabolism NRA Reagent Kits, Diagnostic Reproducibility of Results Tuberculosis - drug therapy Tuberculosis - microbiology Tuberculosis and atypical mycobacterial infections |
title | Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay |
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