Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay

A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportio...

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Veröffentlicht in:Diagnostic microbiology and infectious disease 2005-10, Vol.53 (2), p.121-124
Hauptverfasser: Kumar, Manoj, Khan, Inshad Ali, Verma, Vijashoor, Kalyan, Narender, Qazi, Giulam Nabi
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container_end_page 124
container_issue 2
container_start_page 121
container_title Diagnostic microbiology and infectious disease
container_volume 53
creator Kumar, Manoj
Khan, Inshad Ali
Verma, Vijashoor
Kalyan, Narender
Qazi, Giulam Nabi
description A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for M. tuberculosis makes it more suitable method for MIC determination.
doi_str_mv 10.1016/j.diagmicrobio.2005.05.011
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subjects ABA
Agar proportion method
Antitubercular Agents - pharmacology
Antitubercular Agents - therapeutic use
Bacterial diseases
Biological and medical sciences
Biological Assay - methods
Fundamental and applied biological sciences. Psychology
Human bacterial diseases
Humans
Infectious diseases
Medical sciences
MIC
Microbial Sensitivity Tests
Microbiology
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - enzymology
Mycobacterium tuberculosis - metabolism
Nitrate Reductase - analysis
Nitrate Reductase - metabolism
Nitrates - metabolism
NRA
Reagent Kits, Diagnostic
Reproducibility of Results
Tuberculosis - drug therapy
Tuberculosis - microbiology
Tuberculosis and atypical mycobacterial infections
title Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay
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