Novel function of β-arrestin2 in the nucleus of mature spermatozoa
A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that β-arrestin2, which typically shows a cytoplasmic localization owing to const...
Gespeichert in:
| Veröffentlicht in: | Journal of cell science 2006-08, Vol.119 (15), p.3047-3056 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3056 |
|---|---|
| container_issue | 15 |
| container_start_page | 3047 |
| container_title | Journal of cell science |
| container_volume | 119 |
| creator | Neuhaus, Eva M Mashukova, Anastasia Barbour, Jon Wolters, Dirk Hatt, Hanns |
| description | A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that β-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear export, appears in the nucleus after stimulation of the G-protein-coupled odorant receptor hOR17-4. In the nucleus, β-arrestin2 was involved in transcriptional regulation as shown by a Gal4-based transactivation assay. Moreover, we discovered that β-arrestin2 and hOR17-4, a receptor known to have a role in sperm-egg communication, colocalize in the midpiece of mature human spermatozoa. Stimulation of hOR17-4 in spermatozoa induced PKA-dependent translocation of β-arrestin2 to the nucleus and nuclear accumulation of phosphorylated MAPKs. Analysis of the interaction partners of β-arrestin2 indicates that odorant receptor signaling in spermatozoa may be important for the regulation of gene expression during the early processes of fertilization. |
| doi_str_mv | 10.1242/jcs.03046 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68675860</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68675860</sourcerecordid><originalsourceid>FETCH-LOGICAL-c342t-1d2c6e96b475b01e7ab52f6b37b352e49282ed9cb55a314ca7bdd179b3b76aa43</originalsourceid><addsrcrecordid>eNpFkM1KAzEUhYMotlYXvoDOSnAx9eZ_spTiHxRdaNchyWR0ynRSkxlBH8sH8Zmc2oKre-B-HA4fQqcYppgwcrV0aQoUmNhDY8ykzBWmch-NAQjOFad0hI5SWgKAJEoeohEWBQGGYYxmj-HDN1nVt66rQ5uFKvv5zk2MPnV1S7K6zbo3n7W9a3yfNu-V6fros7T2cYjhK5hjdFCZJvmT3Z2gxe3Ny-w-nz_dPcyu57mjjHQ5LokTXgnLJLeAvTSWk0pYKi3lxDNFCuJL5SznhmLmjLRliaWy1EphDKMTdLHtXcfw3g8D9apOzjeNaX3okxaFkLwQMICXW9DFkFL0lV7HemXip8agN8b0YEz_GRvYs11pb1e-_Cd3igbgfAtUJmjzGuukF88EMAUMCgOX9Bcu7XAc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68675860</pqid></control><display><type>article</type><title>Novel function of β-arrestin2 in the nucleus of mature spermatozoa</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Company of Biologists</source><creator>Neuhaus, Eva M ; Mashukova, Anastasia ; Barbour, Jon ; Wolters, Dirk ; Hatt, Hanns</creator><creatorcontrib>Neuhaus, Eva M ; Mashukova, Anastasia ; Barbour, Jon ; Wolters, Dirk ; Hatt, Hanns</creatorcontrib><description>A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that β-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear export, appears in the nucleus after stimulation of the G-protein-coupled odorant receptor hOR17-4. In the nucleus, β-arrestin2 was involved in transcriptional regulation as shown by a Gal4-based transactivation assay. Moreover, we discovered that β-arrestin2 and hOR17-4, a receptor known to have a role in sperm-egg communication, colocalize in the midpiece of mature human spermatozoa. Stimulation of hOR17-4 in spermatozoa induced PKA-dependent translocation of β-arrestin2 to the nucleus and nuclear accumulation of phosphorylated MAPKs. Analysis of the interaction partners of β-arrestin2 indicates that odorant receptor signaling in spermatozoa may be important for the regulation of gene expression during the early processes of fertilization.</description><identifier>ISSN: 0021-9533</identifier><identifier>EISSN: 1477-9137</identifier><identifier>DOI: 10.1242/jcs.03046</identifier><identifier>PMID: 16820410</identifier><language>eng</language><publisher>England: The Company of Biologists Limited</publisher><subject>Aldehydes - metabolism ; Arrestins - metabolism ; beta-Arrestins ; Cell Line ; Cell Nucleus - metabolism ; Cyclic AMP-Dependent Protein Kinases - metabolism ; Enzyme Activation ; Humans ; Ligands ; Male ; Mitogen-Activated Protein Kinases - metabolism ; Receptors, Odorant - metabolism ; Seminal Plasma Proteins - metabolism ; Spermatozoa - cytology ; Spermatozoa - metabolism ; Transcription, Genetic ; Transcriptional Activation</subject><ispartof>Journal of cell science, 2006-08, Vol.119 (15), p.3047-3056</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-1d2c6e96b475b01e7ab52f6b37b352e49282ed9cb55a314ca7bdd179b3b76aa43</citedby><cites>FETCH-LOGICAL-c342t-1d2c6e96b475b01e7ab52f6b37b352e49282ed9cb55a314ca7bdd179b3b76aa43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3678,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16820410$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neuhaus, Eva M</creatorcontrib><creatorcontrib>Mashukova, Anastasia</creatorcontrib><creatorcontrib>Barbour, Jon</creatorcontrib><creatorcontrib>Wolters, Dirk</creatorcontrib><creatorcontrib>Hatt, Hanns</creatorcontrib><title>Novel function of β-arrestin2 in the nucleus of mature spermatozoa</title><title>Journal of cell science</title><addtitle>J Cell Sci</addtitle><description>A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that β-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear export, appears in the nucleus after stimulation of the G-protein-coupled odorant receptor hOR17-4. In the nucleus, β-arrestin2 was involved in transcriptional regulation as shown by a Gal4-based transactivation assay. Moreover, we discovered that β-arrestin2 and hOR17-4, a receptor known to have a role in sperm-egg communication, colocalize in the midpiece of mature human spermatozoa. Stimulation of hOR17-4 in spermatozoa induced PKA-dependent translocation of β-arrestin2 to the nucleus and nuclear accumulation of phosphorylated MAPKs. Analysis of the interaction partners of β-arrestin2 indicates that odorant receptor signaling in spermatozoa may be important for the regulation of gene expression during the early processes of fertilization.</description><subject>Aldehydes - metabolism</subject><subject>Arrestins - metabolism</subject><subject>beta-Arrestins</subject><subject>Cell Line</subject><subject>Cell Nucleus - metabolism</subject><subject>Cyclic AMP-Dependent Protein Kinases - metabolism</subject><subject>Enzyme Activation</subject><subject>Humans</subject><subject>Ligands</subject><subject>Male</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Receptors, Odorant - metabolism</subject><subject>Seminal Plasma Proteins - metabolism</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Activation</subject><issn>0021-9533</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1KAzEUhYMotlYXvoDOSnAx9eZ_spTiHxRdaNchyWR0ynRSkxlBH8sH8Zmc2oKre-B-HA4fQqcYppgwcrV0aQoUmNhDY8ykzBWmch-NAQjOFad0hI5SWgKAJEoeohEWBQGGYYxmj-HDN1nVt66rQ5uFKvv5zk2MPnV1S7K6zbo3n7W9a3yfNu-V6fros7T2cYjhK5hjdFCZJvmT3Z2gxe3Ny-w-nz_dPcyu57mjjHQ5LokTXgnLJLeAvTSWk0pYKi3lxDNFCuJL5SznhmLmjLRliaWy1EphDKMTdLHtXcfw3g8D9apOzjeNaX3okxaFkLwQMICXW9DFkFL0lV7HemXip8agN8b0YEz_GRvYs11pb1e-_Cd3igbgfAtUJmjzGuukF88EMAUMCgOX9Bcu7XAc</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Neuhaus, Eva M</creator><creator>Mashukova, Anastasia</creator><creator>Barbour, Jon</creator><creator>Wolters, Dirk</creator><creator>Hatt, Hanns</creator><general>The Company of Biologists Limited</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>Novel function of β-arrestin2 in the nucleus of mature spermatozoa</title><author>Neuhaus, Eva M ; Mashukova, Anastasia ; Barbour, Jon ; Wolters, Dirk ; Hatt, Hanns</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-1d2c6e96b475b01e7ab52f6b37b352e49282ed9cb55a314ca7bdd179b3b76aa43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aldehydes - metabolism</topic><topic>Arrestins - metabolism</topic><topic>beta-Arrestins</topic><topic>Cell Line</topic><topic>Cell Nucleus - metabolism</topic><topic>Cyclic AMP-Dependent Protein Kinases - metabolism</topic><topic>Enzyme Activation</topic><topic>Humans</topic><topic>Ligands</topic><topic>Male</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Receptors, Odorant - metabolism</topic><topic>Seminal Plasma Proteins - metabolism</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neuhaus, Eva M</creatorcontrib><creatorcontrib>Mashukova, Anastasia</creatorcontrib><creatorcontrib>Barbour, Jon</creatorcontrib><creatorcontrib>Wolters, Dirk</creatorcontrib><creatorcontrib>Hatt, Hanns</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cell science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neuhaus, Eva M</au><au>Mashukova, Anastasia</au><au>Barbour, Jon</au><au>Wolters, Dirk</au><au>Hatt, Hanns</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel function of β-arrestin2 in the nucleus of mature spermatozoa</atitle><jtitle>Journal of cell science</jtitle><addtitle>J Cell Sci</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>119</volume><issue>15</issue><spage>3047</spage><epage>3056</epage><pages>3047-3056</pages><issn>0021-9533</issn><eissn>1477-9137</eissn><abstract>A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that β-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear export, appears in the nucleus after stimulation of the G-protein-coupled odorant receptor hOR17-4. In the nucleus, β-arrestin2 was involved in transcriptional regulation as shown by a Gal4-based transactivation assay. Moreover, we discovered that β-arrestin2 and hOR17-4, a receptor known to have a role in sperm-egg communication, colocalize in the midpiece of mature human spermatozoa. Stimulation of hOR17-4 in spermatozoa induced PKA-dependent translocation of β-arrestin2 to the nucleus and nuclear accumulation of phosphorylated MAPKs. Analysis of the interaction partners of β-arrestin2 indicates that odorant receptor signaling in spermatozoa may be important for the regulation of gene expression during the early processes of fertilization.</abstract><cop>England</cop><pub>The Company of Biologists Limited</pub><pmid>16820410</pmid><doi>10.1242/jcs.03046</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9533 |
| ispartof | Journal of cell science, 2006-08, Vol.119 (15), p.3047-3056 |
| issn | 0021-9533 1477-9137 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68675860 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Company of Biologists |
| subjects | Aldehydes - metabolism Arrestins - metabolism beta-Arrestins Cell Line Cell Nucleus - metabolism Cyclic AMP-Dependent Protein Kinases - metabolism Enzyme Activation Humans Ligands Male Mitogen-Activated Protein Kinases - metabolism Receptors, Odorant - metabolism Seminal Plasma Proteins - metabolism Spermatozoa - cytology Spermatozoa - metabolism Transcription, Genetic Transcriptional Activation |
| title | Novel function of β-arrestin2 in the nucleus of mature spermatozoa |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T23%3A28%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20function%20of%20%CE%B2-arrestin2%20in%20the%20nucleus%20of%20mature%20spermatozoa&rft.jtitle=Journal%20of%20cell%20science&rft.au=Neuhaus,%20Eva%20M&rft.date=2006-08-01&rft.volume=119&rft.issue=15&rft.spage=3047&rft.epage=3056&rft.pages=3047-3056&rft.issn=0021-9533&rft.eissn=1477-9137&rft_id=info:doi/10.1242/jcs.03046&rft_dat=%3Cproquest_cross%3E68675860%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68675860&rft_id=info:pmid/16820410&rfr_iscdi=true |