In Vitro Expansion of Human Mesenchymal Stem Cells: Choice of Serum Is a Determinant of Cell Proliferation, Differentiation, Gene Expression, and Transcriptome Stability

Human bone marrow mesenchymal stem cells (hMSCs) represent an appealing source of adult stem cells for cell therapy and tissue engineering, as they are easily obtained and expanded while maintaining their multilineage differentiation potential. All current protocols for in vitro culture of hMSCs inc...

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Veröffentlicht in:	Stem cells (Dayton, Ohio) Ohio), 2005-10, Vol.23 (9), p.1357-1366
Hauptverfasser:	Shahdadfar, Aboulghassem, Frønsdal, Katrine, Haug, Terje, Reinholt, Finn P., Brinchmann, Jan E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Autologous serum Bone Marrow Cells - cytology Cattle Cell culture Cell Culture Techniques - methods Cell Differentiation - physiology Cell Growth Processes - physiology Culture Media Fetal bovine serum Gene expression Gene Expression Profiling Gene Expression Regulation Human mesenchymal stem cells Humans In vitro differentiation Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Microarray Reverse Transcriptase Polymerase Chain Reaction
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