Early detection of Schistosoma mansoni infection by touchdown PCR in a mouse model
A detection assay for Schistosoma mansoni DNA in mouse serum samples based on touchdown PCR was developed and evaluated. The serum samples could be assayed directly without the need to extract DNA. No cross reactions between S. mansoni and related species inducing human schistosomiasis were observed...
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| Veröffentlicht in: | Parasitology international 2006-09, Vol.55 (3), p.213-218 |
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| container_title | Parasitology international |
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| creator | Suzuki, Tomoyuki Osada, Yoshio Kumagai, Takashi Hamada, Atsuo Okuzawa, Eiichi Kanazawa, Tamotsu |
| description | A detection assay for
Schistosoma mansoni DNA in mouse serum samples based on touchdown PCR was developed and evaluated. The serum samples could be assayed directly without the need to extract DNA. No cross reactions between
S. mansoni and related species inducing human schistosomiasis were observed. After the infection, mouse sera and feces were collected for 8
weeks. Anti-worm antigen IgG and anti-soluble egg antigen IgG were detected in the sera at 6
weeks post-infection by ELISA. The parasite's eggs were detected in the feces at 8
weeks. In contrast,
S. mansoni DNA was detected in the sera at 2
weeks post-infection. These data suggest that touchdown PCR is a potential tool for the early diagnosis of
S. mansoni infection. |
| doi_str_mv | 10.1016/j.parint.2006.05.004 |
| format | Article |
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Schistosoma mansoni DNA in mouse serum samples based on touchdown PCR was developed and evaluated. The serum samples could be assayed directly without the need to extract DNA. No cross reactions between
S. mansoni and related species inducing human schistosomiasis were observed. After the infection, mouse sera and feces were collected for 8
weeks. Anti-worm antigen IgG and anti-soluble egg antigen IgG were detected in the sera at 6
weeks post-infection by ELISA. The parasite's eggs were detected in the feces at 8
weeks. In contrast,
S. mansoni DNA was detected in the sera at 2
weeks post-infection. These data suggest that touchdown PCR is a potential tool for the early diagnosis of
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Schistosoma mansoni DNA in mouse serum samples based on touchdown PCR was developed and evaluated. The serum samples could be assayed directly without the need to extract DNA. No cross reactions between
S. mansoni and related species inducing human schistosomiasis were observed. After the infection, mouse sera and feces were collected for 8
weeks. Anti-worm antigen IgG and anti-soluble egg antigen IgG were detected in the sera at 6
weeks post-infection by ELISA. The parasite's eggs were detected in the feces at 8
weeks. In contrast,
S. mansoni DNA was detected in the sera at 2
weeks post-infection. These data suggest that touchdown PCR is a potential tool for the early diagnosis of
S. mansoni infection.</description><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antibodies, Helminth - immunology</subject><subject>Antigens, Helminth - immunology</subject><subject>Diagnosis</subject><subject>Early Diagnosis</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - immunology</subject><subject>Mice</subject><subject>Parasite Egg Count</subject><subject>PCR</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - genetics</subject><subject>Schistosoma mansoni - immunology</subject><subject>Schistosoma mansoni - isolation & purification</subject><subject>Schistosomiasis</subject><subject>Schistosomiasis mansoni - diagnosis</subject><subject>Sensitivity and Specificity</subject><issn>1383-5769</issn><issn>1873-0329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMofv8DkZ68tU7S5mMvgix-gaCseg4xmWKWtlmTrrL_3iy74E0vSZg8887wEHJGoaJAxeW8Wpjoh7FiAKICXgE0O-SQKlmXULPJbn7Xqi65FJMDcpTSHIByKek-OaBCMSZBHZLZjYndqnA4oh19GIrQFi_2w6cxpNCbojdDCoMv_NBugfdVMYal_XDheyiep7P8VWQuLBPm02F3QvZa0yU83d7H5O325nV6Xz4-3T1Mrx9LyymMJRpoGApKW4HSghMgjTRWomJ5fdVSaZh1yqHgFGunJtI2uc7RNI2tW1ofk4tN7iKGzyWmUfc-Wew6M2DeRgslhKJ88i9IJRM1ZzyDzQa0MaQUsdWL6HsTV5qCXkvXc72RrtfSNXCdpee2823-8r1H99u0tZyBqw2AWceXx6iT9ThYdD5mq9oF__eEH1belLc</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>Suzuki, Tomoyuki</creator><creator>Osada, Yoshio</creator><creator>Kumagai, Takashi</creator><creator>Hamada, Atsuo</creator><creator>Okuzawa, Eiichi</creator><creator>Kanazawa, Tamotsu</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>Early detection of Schistosoma mansoni infection by touchdown PCR in a mouse model</title><author>Suzuki, Tomoyuki ; Osada, Yoshio ; Kumagai, Takashi ; Hamada, Atsuo ; Okuzawa, Eiichi ; Kanazawa, Tamotsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-ea042e611f6e7c0d607a7ac7e820328f17a2cd8de651e3d897c43285ea44c3f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antibodies, Helminth - immunology</topic><topic>Antigens, Helminth - immunology</topic><topic>Diagnosis</topic><topic>Early Diagnosis</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin G - immunology</topic><topic>Mice</topic><topic>Parasite Egg Count</topic><topic>PCR</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - genetics</topic><topic>Schistosoma mansoni - immunology</topic><topic>Schistosoma mansoni - isolation & purification</topic><topic>Schistosomiasis</topic><topic>Schistosomiasis mansoni - diagnosis</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Tomoyuki</creatorcontrib><creatorcontrib>Osada, Yoshio</creatorcontrib><creatorcontrib>Kumagai, Takashi</creatorcontrib><creatorcontrib>Hamada, Atsuo</creatorcontrib><creatorcontrib>Okuzawa, Eiichi</creatorcontrib><creatorcontrib>Kanazawa, Tamotsu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Tomoyuki</au><au>Osada, Yoshio</au><au>Kumagai, Takashi</au><au>Hamada, Atsuo</au><au>Okuzawa, Eiichi</au><au>Kanazawa, Tamotsu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early detection of Schistosoma mansoni infection by touchdown PCR in a mouse model</atitle><jtitle>Parasitology international</jtitle><addtitle>Parasitol Int</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>55</volume><issue>3</issue><spage>213</spage><epage>218</epage><pages>213-218</pages><issn>1383-5769</issn><eissn>1873-0329</eissn><abstract>A detection assay for
Schistosoma mansoni DNA in mouse serum samples based on touchdown PCR was developed and evaluated. The serum samples could be assayed directly without the need to extract DNA. No cross reactions between
S. mansoni and related species inducing human schistosomiasis were observed. After the infection, mouse sera and feces were collected for 8
weeks. Anti-worm antigen IgG and anti-soluble egg antigen IgG were detected in the sera at 6
weeks post-infection by ELISA. The parasite's eggs were detected in the feces at 8
weeks. In contrast,
S. mansoni DNA was detected in the sera at 2
weeks post-infection. These data suggest that touchdown PCR is a potential tool for the early diagnosis of
S. mansoni infection.</abstract><cop>Netherlands</cop><pub>Elsevier Ireland Ltd</pub><pmid>16822708</pmid><doi>10.1016/j.parint.2006.05.004</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 1383-5769 |
| ispartof | Parasitology international, 2006-09, Vol.55 (3), p.213-218 |
| issn | 1383-5769 1873-0329 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antibodies, Helminth - blood Antibodies, Helminth - immunology Antigens, Helminth - immunology Diagnosis Early Diagnosis Enzyme-Linked Immunosorbent Assay Feces - parasitology Female Immunoglobulin G - blood Immunoglobulin G - immunology Mice Parasite Egg Count PCR Polymerase Chain Reaction - methods Schistosoma mansoni Schistosoma mansoni - genetics Schistosoma mansoni - immunology Schistosoma mansoni - isolation & purification Schistosomiasis Schistosomiasis mansoni - diagnosis Sensitivity and Specificity |
| title | Early detection of Schistosoma mansoni infection by touchdown PCR in a mouse model |
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