Expression and Comparative Analysis of Genes Encoding Outer Membrane Proteins LipL21, LipL32 and OmpL1 in Epidemic Leptospires
Leptospiral outer membrane proteins (OMPs) are highly conserved in different species, and play an essential role in the development of new immunoprotection and serodiagnosis strategies. The genes encoding LipL21, LipL32 and OmpL1 were cloned from the complete genome sequence of Leptospira interrogan...
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| Veröffentlicht in: | Acta biochimica et biophysica Sinica 2005-10, Vol.37 (10), p.649-656 |
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| container_title | Acta biochimica et biophysica Sinica |
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| creator | ZHANG, Xiang‐Yan YU, Yang HE, Ping ZHANG, Yi‐Xuan HU, Bao‐Yu YANG, Yang NIE, Yi‐Xin JIANG, Xiu‐Gao ZHAO, Guo‐Ping GUO, Xiao‐Kui |
| description | Leptospiral outer membrane proteins (OMPs) are highly conserved in different species, and play an essential role in the development of new immunoprotection and serodiagnosis strategies. The genes encoding LipL21, LipL32 and OmpL1 were cloned from the complete genome sequence of Leptospira interrogans serovar lai strain Lai and expressed in vitro. Sequence comparison analysis revealed that the three genes were highly conserved among distinct epidemic leptospires, including three major epidemic species Leptospira interrogans, Leptospira borgpetersenii and Leptospira weilii, in China. Immunoblot analysis was further performed to scrutinize 15 epidemic Leptospira reference strains using the antisera of the recombinant OMPs. Both immunoblot assay and reverse transcription‐polymerase chain reaction demonstrated that these three OMPs were conservatively expressed in pathogenic L. interrogans strains and other pathogenic leptospires. Additionally, the use of these recombinant OMPs as antigens in enzyme‐linked immunosorbent assay (ELISA) for serodiagnosis of leptospirosis was evaluated. The recombinant LipL32 and OmpL1 proteins showed a high degree of ELISA reactivity with sera from patients infected with L. interrogans strain Lai and other pathogenic leptospires. These results may contribute to the identification of candidates for broad‐range vaccines and immunodiagnostic antigens in further research.
Edited by
Xiang‐Fu WU |
| doi_str_mv | 10.1111/j.1745-7270.2005.00094.x |
| format | Article |
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Edited by
Xiang‐Fu WU</description><identifier>ISSN: 1672-9145</identifier><identifier>EISSN: 1745-7270</identifier><identifier>DOI: 10.1111/j.1745-7270.2005.00094.x</identifier><identifier>PMID: 16215631</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Pty</publisher><subject>Antigens, Bacterial - immunology ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Outer Membrane Proteins - immunology ; Blotting, Western ; comparative analysis ; epidemic leptospire ; expression ; Genes, Bacterial ; Leptospira - genetics ; Leptospira borgpetersenii ; Leptospira interrogans ; Leptospira interrogans - genetics ; Leptospira weilii ; Leptospirosis - immunology ; Lipoproteins - genetics ; outer membrane protein ; Recombinant Proteins - analysis</subject><ispartof>Acta biochimica et biophysica Sinica, 2005-10, Vol.37 (10), p.649-656</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3984-b275aa9b096b0054c3a96b2da2697d567af164cfb4644a1ba734caabcc52b8893</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1745-7270.2005.00094.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1745-7270.2005.00094.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16215631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ZHANG, Xiang‐Yan</creatorcontrib><creatorcontrib>YU, Yang</creatorcontrib><creatorcontrib>HE, Ping</creatorcontrib><creatorcontrib>ZHANG, Yi‐Xuan</creatorcontrib><creatorcontrib>HU, Bao‐Yu</creatorcontrib><creatorcontrib>YANG, Yang</creatorcontrib><creatorcontrib>NIE, Yi‐Xin</creatorcontrib><creatorcontrib>JIANG, Xiu‐Gao</creatorcontrib><creatorcontrib>ZHAO, Guo‐Ping</creatorcontrib><creatorcontrib>GUO, Xiao‐Kui</creatorcontrib><title>Expression and Comparative Analysis of Genes Encoding Outer Membrane Proteins LipL21, LipL32 and OmpL1 in Epidemic Leptospires</title><title>Acta biochimica et biophysica Sinica</title><addtitle>Acta Biochim Biophys Sin (Shanghai)</addtitle><description>Leptospiral outer membrane proteins (OMPs) are highly conserved in different species, and play an essential role in the development of new immunoprotection and serodiagnosis strategies. The genes encoding LipL21, LipL32 and OmpL1 were cloned from the complete genome sequence of Leptospira interrogans serovar lai strain Lai and expressed in vitro. Sequence comparison analysis revealed that the three genes were highly conserved among distinct epidemic leptospires, including three major epidemic species Leptospira interrogans, Leptospira borgpetersenii and Leptospira weilii, in China. Immunoblot analysis was further performed to scrutinize 15 epidemic Leptospira reference strains using the antisera of the recombinant OMPs. Both immunoblot assay and reverse transcription‐polymerase chain reaction demonstrated that these three OMPs were conservatively expressed in pathogenic L. interrogans strains and other pathogenic leptospires. Additionally, the use of these recombinant OMPs as antigens in enzyme‐linked immunosorbent assay (ELISA) for serodiagnosis of leptospirosis was evaluated. The recombinant LipL32 and OmpL1 proteins showed a high degree of ELISA reactivity with sera from patients infected with L. interrogans strain Lai and other pathogenic leptospires. These results may contribute to the identification of candidates for broad‐range vaccines and immunodiagnostic antigens in further research.
Edited by
Xiang‐Fu WU</description><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Outer Membrane Proteins - immunology</subject><subject>Blotting, Western</subject><subject>comparative analysis</subject><subject>epidemic leptospire</subject><subject>expression</subject><subject>Genes, Bacterial</subject><subject>Leptospira - genetics</subject><subject>Leptospira borgpetersenii</subject><subject>Leptospira interrogans</subject><subject>Leptospira interrogans - genetics</subject><subject>Leptospira weilii</subject><subject>Leptospirosis - immunology</subject><subject>Lipoproteins - genetics</subject><subject>outer membrane protein</subject><subject>Recombinant Proteins - analysis</subject><issn>1672-9145</issn><issn>1745-7270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-v1CAUxYnR-J5Pv4Jh5cpWoBTKwsW8yfg0qRkTdU2AUsOkBYSOzmz87DJ_oku9m3sSzj2Xmx8AEKMal3qzqzGnbcUJRzVBqK0RQoLWh0fg9s_D46IZJ5XAtL0Bz3LeIdQwhtFTcIMZwS1r8C34tTnEZHN2wUPlB7gOc1RJLe6HhSuvpmN2GYYRPlhvM9x4Ewbnv8HtfrEJfrSzTspb-CmFxTqfYe9iT_Drc2_IOXE7xx5D5-EmusHOzsDexiXk6Mre5-DJqKZsX1z7Hfj6bvNl_b7qtw8f1qu-Mo3oaKUJb5USGgmmy7nUNKooMijCBB9axtWIGTWjpoxShbXiDTVKaWNaortONHfg1SU3pvB9b_MiZ5eNnaby-7DPknWMcY75P41YEIEopcXYXYwmhZyTHWVMblbpKDGSJ0hyJ08s5ImFPEGSZ0jyUEZfXnfs9WyHv4NXKsXw9mL46SZ7_O9gubq__1xU8xs5op_p</recordid><startdate>200510</startdate><enddate>200510</enddate><creator>ZHANG, Xiang‐Yan</creator><creator>YU, Yang</creator><creator>HE, Ping</creator><creator>ZHANG, Yi‐Xuan</creator><creator>HU, Bao‐Yu</creator><creator>YANG, Yang</creator><creator>NIE, Yi‐Xin</creator><creator>JIANG, Xiu‐Gao</creator><creator>ZHAO, Guo‐Ping</creator><creator>GUO, Xiao‐Kui</creator><general>Blackwell Science Pty</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200510</creationdate><title>Expression and Comparative Analysis of Genes Encoding Outer Membrane Proteins LipL21, LipL32 and OmpL1 in Epidemic Leptospires</title><author>ZHANG, Xiang‐Yan ; YU, Yang ; HE, Ping ; ZHANG, Yi‐Xuan ; HU, Bao‐Yu ; YANG, Yang ; NIE, Yi‐Xin ; JIANG, Xiu‐Gao ; ZHAO, Guo‐Ping ; GUO, Xiao‐Kui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3984-b275aa9b096b0054c3a96b2da2697d567af164cfb4644a1ba734caabcc52b8893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Bacterial Outer Membrane Proteins - immunology</topic><topic>Blotting, Western</topic><topic>comparative analysis</topic><topic>epidemic leptospire</topic><topic>expression</topic><topic>Genes, Bacterial</topic><topic>Leptospira - genetics</topic><topic>Leptospira borgpetersenii</topic><topic>Leptospira interrogans</topic><topic>Leptospira interrogans - genetics</topic><topic>Leptospira weilii</topic><topic>Leptospirosis - immunology</topic><topic>Lipoproteins - genetics</topic><topic>outer membrane protein</topic><topic>Recombinant Proteins - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ZHANG, Xiang‐Yan</creatorcontrib><creatorcontrib>YU, Yang</creatorcontrib><creatorcontrib>HE, Ping</creatorcontrib><creatorcontrib>ZHANG, Yi‐Xuan</creatorcontrib><creatorcontrib>HU, Bao‐Yu</creatorcontrib><creatorcontrib>YANG, Yang</creatorcontrib><creatorcontrib>NIE, Yi‐Xin</creatorcontrib><creatorcontrib>JIANG, Xiu‐Gao</creatorcontrib><creatorcontrib>ZHAO, Guo‐Ping</creatorcontrib><creatorcontrib>GUO, Xiao‐Kui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biochimica et biophysica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ZHANG, Xiang‐Yan</au><au>YU, Yang</au><au>HE, Ping</au><au>ZHANG, Yi‐Xuan</au><au>HU, Bao‐Yu</au><au>YANG, Yang</au><au>NIE, Yi‐Xin</au><au>JIANG, Xiu‐Gao</au><au>ZHAO, Guo‐Ping</au><au>GUO, Xiao‐Kui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and Comparative Analysis of Genes Encoding Outer Membrane Proteins LipL21, LipL32 and OmpL1 in Epidemic Leptospires</atitle><jtitle>Acta biochimica et biophysica Sinica</jtitle><addtitle>Acta Biochim Biophys Sin (Shanghai)</addtitle><date>2005-10</date><risdate>2005</risdate><volume>37</volume><issue>10</issue><spage>649</spage><epage>656</epage><pages>649-656</pages><issn>1672-9145</issn><eissn>1745-7270</eissn><abstract>Leptospiral outer membrane proteins (OMPs) are highly conserved in different species, and play an essential role in the development of new immunoprotection and serodiagnosis strategies. The genes encoding LipL21, LipL32 and OmpL1 were cloned from the complete genome sequence of Leptospira interrogans serovar lai strain Lai and expressed in vitro. Sequence comparison analysis revealed that the three genes were highly conserved among distinct epidemic leptospires, including three major epidemic species Leptospira interrogans, Leptospira borgpetersenii and Leptospira weilii, in China. Immunoblot analysis was further performed to scrutinize 15 epidemic Leptospira reference strains using the antisera of the recombinant OMPs. Both immunoblot assay and reverse transcription‐polymerase chain reaction demonstrated that these three OMPs were conservatively expressed in pathogenic L. interrogans strains and other pathogenic leptospires. Additionally, the use of these recombinant OMPs as antigens in enzyme‐linked immunosorbent assay (ELISA) for serodiagnosis of leptospirosis was evaluated. The recombinant LipL32 and OmpL1 proteins showed a high degree of ELISA reactivity with sera from patients infected with L. interrogans strain Lai and other pathogenic leptospires. These results may contribute to the identification of candidates for broad‐range vaccines and immunodiagnostic antigens in further research.
Edited by
Xiang‐Fu WU</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Pty</pub><pmid>16215631</pmid><doi>10.1111/j.1745-7270.2005.00094.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Acta biochimica et biophysica Sinica, 2005-10, Vol.37 (10), p.649-656 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Antigens, Bacterial - immunology Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - immunology Blotting, Western comparative analysis epidemic leptospire expression Genes, Bacterial Leptospira - genetics Leptospira borgpetersenii Leptospira interrogans Leptospira interrogans - genetics Leptospira weilii Leptospirosis - immunology Lipoproteins - genetics outer membrane protein Recombinant Proteins - analysis |
| title | Expression and Comparative Analysis of Genes Encoding Outer Membrane Proteins LipL21, LipL32 and OmpL1 in Epidemic Leptospires |
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