A modified AFLP for Trypanosoma congolense isolate characterisation
The amplified fragment length polymorphism (AFLP) technique is a reliable and powerful DNA fingerprint tool for genetic characterisation and analysis. In this paper, we described a modified AFLP with high resolution for Trypanosoma congolense using one enzyme and agarose or Elchrom gel electrophores...
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| Veröffentlicht in: | Journal of biotechnology 2006-08, Vol.125 (1), p.22-26 |
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| creator | Masumu, J. Geysen, D. Vansnick, E. Geerts, S. Van den Bossche, P. |
| description | The amplified fragment length polymorphism (AFLP) technique is a reliable and powerful DNA fingerprint tool for genetic characterisation and analysis. In this paper, we described a modified AFLP with high resolution for
Trypanosoma congolense using one enzyme and agarose or Elchrom gel electrophoresis. Eleven allopatric and fourteen sympatric isolates of
T. congolense savannah were used to assess the resolution of the method and its ability to characterise
T. congolense isolates. Two enzymes (
Eco RI or
Bgl II) and corresponding non-selective and selective primers were used to identify the most appropriate combination. Patterns generated by
Bgl II enzyme and a single selective primer A, C, G or T produced clear profiles. Each of the four selective primers produced different profiles for all the 25
T. congolense isolates. Due to the reduction in the number of bands, profiles could be analysed using agarose or Elchrom gels.
Although comparison of a great number of samples could benefit from software help, this technique did not require flurochrome detection methods. The results of the present study demonstrated that this modified AFLP makes the characterisation of
T. congolense easier while maintaining high resolution. |
| doi_str_mv | 10.1016/j.jbiotec.2006.01.016 |
| format | Article |
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Trypanosoma congolense using one enzyme and agarose or Elchrom gel electrophoresis. Eleven allopatric and fourteen sympatric isolates of
T. congolense savannah were used to assess the resolution of the method and its ability to characterise
T. congolense isolates. Two enzymes (
Eco RI or
Bgl II) and corresponding non-selective and selective primers were used to identify the most appropriate combination. Patterns generated by
Bgl II enzyme and a single selective primer A, C, G or T produced clear profiles. Each of the four selective primers produced different profiles for all the 25
T. congolense isolates. Due to the reduction in the number of bands, profiles could be analysed using agarose or Elchrom gels.
Although comparison of a great number of samples could benefit from software help, this technique did not require flurochrome detection methods. The results of the present study demonstrated that this modified AFLP makes the characterisation of
T. congolense easier while maintaining high resolution.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/j.jbiotec.2006.01.016</identifier><identifier>PMID: 16516323</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>AFLP fingerprinting ; Animals ; Biological and medical sciences ; Biotechnology ; Characterisation ; Deoxyribonucleases, Type II Site-Specific - metabolism ; DNA Primers - genetics ; DNA, Protozoan - genetics ; DNA, Protozoan - metabolism ; Electrophoresis - methods ; Electrophoresis, Agar Gel - methods ; Fundamental and applied biological sciences. Psychology ; Genetic diversity ; Nucleic Acid Amplification Techniques - methods ; Reproducibility of Results ; Trypanosoma congolense ; Trypanosoma congolense - classification ; Trypanosoma congolense - genetics ; Trypanosoma congolense - isolation & purification</subject><ispartof>Journal of biotechnology, 2006-08, Vol.125 (1), p.22-26</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-b3601b01b225a408c20372fcacf10bcd83f49f93cf7a8d4bb8778173312d47263</citedby><cites>FETCH-LOGICAL-c424t-b3601b01b225a408c20372fcacf10bcd83f49f93cf7a8d4bb8778173312d47263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jbiotec.2006.01.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17989193$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16516323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Masumu, J.</creatorcontrib><creatorcontrib>Geysen, D.</creatorcontrib><creatorcontrib>Vansnick, E.</creatorcontrib><creatorcontrib>Geerts, S.</creatorcontrib><creatorcontrib>Van den Bossche, P.</creatorcontrib><title>A modified AFLP for Trypanosoma congolense isolate characterisation</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>The amplified fragment length polymorphism (AFLP) technique is a reliable and powerful DNA fingerprint tool for genetic characterisation and analysis. In this paper, we described a modified AFLP with high resolution for
Trypanosoma congolense using one enzyme and agarose or Elchrom gel electrophoresis. Eleven allopatric and fourteen sympatric isolates of
T. congolense savannah were used to assess the resolution of the method and its ability to characterise
T. congolense isolates. Two enzymes (
Eco RI or
Bgl II) and corresponding non-selective and selective primers were used to identify the most appropriate combination. Patterns generated by
Bgl II enzyme and a single selective primer A, C, G or T produced clear profiles. Each of the four selective primers produced different profiles for all the 25
T. congolense isolates. Due to the reduction in the number of bands, profiles could be analysed using agarose or Elchrom gels.
Although comparison of a great number of samples could benefit from software help, this technique did not require flurochrome detection methods. The results of the present study demonstrated that this modified AFLP makes the characterisation of
T. congolense easier while maintaining high resolution.</description><subject>AFLP fingerprinting</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Characterisation</subject><subject>Deoxyribonucleases, Type II Site-Specific - metabolism</subject><subject>DNA Primers - genetics</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Protozoan - metabolism</subject><subject>Electrophoresis - methods</subject><subject>Electrophoresis, Agar Gel - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic diversity</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Reproducibility of Results</subject><subject>Trypanosoma congolense</subject><subject>Trypanosoma congolense - classification</subject><subject>Trypanosoma congolense - genetics</subject><subject>Trypanosoma congolense - isolation & purification</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVJaTZpf0KCL-nNW41kS_KpLEvzAQvtIT0LeTxqtdjWVvIG8u_rZQ05Bl4YGJ754GHsBvgaOKhv-_W-DXEiXAvO1ZrDHPWBrcBoWVZGyQu2mjumBFWrS3aV855zXjU1fGKXcw-UFHLFtptiiF3wgbpic7_7VfiYiuf0enBjzHFwBcbxT-xpzFSEHHs3UYF_XXI4UQrZTSGOn9lH7_pMX5Z6zX7f_3jePpa7nw9P282uxEpUU9lKxaGdI0TtKm5QcKmFR4ceeIudkb5qfCPRa2e6qm2N1ga0lCC6Sgslr9nX895Div-OlCc7hIzU926keMxWGaU0gHgXhEbxWkk-g_UZxBRzTuTtIYXBpVcL3J40271dNNuTZsthzumT2-XAsR2oe5tavM7A3QK4jK73yY0Y8hunG9NAc-K-nzmavb0ESjZjoBGpC4lwsl0M77zyH0AqnKM</recordid><startdate>20060820</startdate><enddate>20060820</enddate><creator>Masumu, J.</creator><creator>Geysen, D.</creator><creator>Vansnick, E.</creator><creator>Geerts, S.</creator><creator>Van den Bossche, P.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060820</creationdate><title>A modified AFLP for Trypanosoma congolense isolate characterisation</title><author>Masumu, J. ; Geysen, D. ; Vansnick, E. ; Geerts, S. ; Van den Bossche, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-b3601b01b225a408c20372fcacf10bcd83f49f93cf7a8d4bb8778173312d47263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>AFLP fingerprinting</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Characterisation</topic><topic>Deoxyribonucleases, Type II Site-Specific - metabolism</topic><topic>DNA Primers - genetics</topic><topic>DNA, Protozoan - genetics</topic><topic>DNA, Protozoan - metabolism</topic><topic>Electrophoresis - methods</topic><topic>Electrophoresis, Agar Gel - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic diversity</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Reproducibility of Results</topic><topic>Trypanosoma congolense</topic><topic>Trypanosoma congolense - classification</topic><topic>Trypanosoma congolense - genetics</topic><topic>Trypanosoma congolense - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Masumu, J.</creatorcontrib><creatorcontrib>Geysen, D.</creatorcontrib><creatorcontrib>Vansnick, E.</creatorcontrib><creatorcontrib>Geerts, S.</creatorcontrib><creatorcontrib>Van den Bossche, P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masumu, J.</au><au>Geysen, D.</au><au>Vansnick, E.</au><au>Geerts, S.</au><au>Van den Bossche, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A modified AFLP for Trypanosoma congolense isolate characterisation</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2006-08-20</date><risdate>2006</risdate><volume>125</volume><issue>1</issue><spage>22</spage><epage>26</epage><pages>22-26</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>The amplified fragment length polymorphism (AFLP) technique is a reliable and powerful DNA fingerprint tool for genetic characterisation and analysis. In this paper, we described a modified AFLP with high resolution for
Trypanosoma congolense using one enzyme and agarose or Elchrom gel electrophoresis. Eleven allopatric and fourteen sympatric isolates of
T. congolense savannah were used to assess the resolution of the method and its ability to characterise
T. congolense isolates. Two enzymes (
Eco RI or
Bgl II) and corresponding non-selective and selective primers were used to identify the most appropriate combination. Patterns generated by
Bgl II enzyme and a single selective primer A, C, G or T produced clear profiles. Each of the four selective primers produced different profiles for all the 25
T. congolense isolates. Due to the reduction in the number of bands, profiles could be analysed using agarose or Elchrom gels.
Although comparison of a great number of samples could benefit from software help, this technique did not require flurochrome detection methods. The results of the present study demonstrated that this modified AFLP makes the characterisation of
T. congolense easier while maintaining high resolution.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>16516323</pmid><doi>10.1016/j.jbiotec.2006.01.016</doi><tpages>5</tpages></addata></record> |
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| subjects | AFLP fingerprinting Animals Biological and medical sciences Biotechnology Characterisation Deoxyribonucleases, Type II Site-Specific - metabolism DNA Primers - genetics DNA, Protozoan - genetics DNA, Protozoan - metabolism Electrophoresis - methods Electrophoresis, Agar Gel - methods Fundamental and applied biological sciences. Psychology Genetic diversity Nucleic Acid Amplification Techniques - methods Reproducibility of Results Trypanosoma congolense Trypanosoma congolense - classification Trypanosoma congolense - genetics Trypanosoma congolense - isolation & purification |
| title | A modified AFLP for Trypanosoma congolense isolate characterisation |
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