Morphological, stereological and radiological changes in pinealectomized chicken cervical vertebrae
: Considerable evidence supports the hypothesis that melatonin plays an important role in osteogenesis. We carried out an experiment to investigate histological and radiological changes in the cervical vertebrae of pinealectomized chickens. Thirty new‐hatched chicks were divided into two groups: pi...
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| Veröffentlicht in: | Journal of pineal research 2005-11, Vol.39 (4), p.392-399 |
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| creator | Turgut, Mehmet Kaplan, Süleyman Turgut, Ahmet T. Aslan, Hüseyin Güvenç, Tolga Çullu, Emre Erdoǧan, Serpil |
| description | : Considerable evidence supports the hypothesis that melatonin plays an important role in osteogenesis. We carried out an experiment to investigate histological and radiological changes in the cervical vertebrae of pinealectomized chickens. Thirty new‐hatched chicks were divided into two groups: pinealectomized group (n = 15) and nonpinealectomized control (n = 15). Surgical pinealectomies were performed in Hybro broiler chickens at the age of 3 days. At 8 wk, one animal from each group was examined using computed tomography scanner to obtain density histograms of four consecutive vertebrae between the 4th and 7th cervical vertebral segments of chickens. At the end of the study, all animals were killed for subsequent measurement of bone mineral density (BMD) and macroscopic, radiographic and histopathological evaluation of specimens. We found that the profile of the density histogram in nonpinealectomized control group showed a spike profile for the vertebral body, indicating an increase in the amount of higher density tissues in this region, while a plateau‐shaped profile was obtained for the vertebral body in pinealectomy group, indicating the presence of heterogenous bone tissue. Accordingly, the mean value of BMD in pinealectomy group was significantly lower at the vertebral body in chickens compared with control subjects (P |
| doi_str_mv | 10.1111/j.1600-079X.2005.00263.x |
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We carried out an experiment to investigate histological and radiological changes in the cervical vertebrae of pinealectomized chickens. Thirty new‐hatched chicks were divided into two groups: pinealectomized group (n = 15) and nonpinealectomized control (n = 15). Surgical pinealectomies were performed in Hybro broiler chickens at the age of 3 days. At 8 wk, one animal from each group was examined using computed tomography scanner to obtain density histograms of four consecutive vertebrae between the 4th and 7th cervical vertebral segments of chickens. At the end of the study, all animals were killed for subsequent measurement of bone mineral density (BMD) and macroscopic, radiographic and histopathological evaluation of specimens. We found that the profile of the density histogram in nonpinealectomized control group showed a spike profile for the vertebral body, indicating an increase in the amount of higher density tissues in this region, while a plateau‐shaped profile was obtained for the vertebral body in pinealectomy group, indicating the presence of heterogenous bone tissue. Accordingly, the mean value of BMD in pinealectomy group was significantly lower at the vertebral body in chickens compared with control subjects (P < 0.001). At the end of the experiment, almost all of the chickens in pinealectomy group developed a scoliotic curvature and the mean weight and length of the cervical vertebral bodies of the pinealectomized chickens were significantly lower than those of control group (P < 0.001). Although the numerical density of osteocytes and osteoblasts in pinealectomy group was significantly higher than that from the control group, total number of osteocytes but not osteoblasts in cervical vetrebrae from pinealectomized animals was significantly lower than that from nonpinealectomized control animals (P < 0.05). In conclusion, the results of the current study demonstrated for the first time pinealectomy‐induced histomorphometrical changes in chicken vertebral column using stereological methods, suggesting that pineal gland/melatonin may have an osteoinductive effect on bone formation, but further studies are needed to elucidate the relationship of our findings with some disorders such as postmenopausal and/or senile osteoporosis.</description><identifier>ISSN: 0742-3098</identifier><identifier>EISSN: 1600-079X</identifier><identifier>DOI: 10.1111/j.1600-079X.2005.00263.x</identifier><identifier>PMID: 16207295</identifier><identifier>CODEN: JPRSE9</identifier><language>eng</language><publisher>Oxford, UK: Munksgaard International Publishers</publisher><subject>Animals ; Biological and medical sciences ; Bone Density ; Cell Count ; Cervical Vertebrae - anatomy & histology ; Cervical Vertebrae - cytology ; Cervical Vertebrae - diagnostic imaging ; Chickens ; computed tomography ; Female ; Fundamental and applied biological sciences. Psychology ; Male ; melatonin ; Organ Size ; Osteoblasts - cytology ; Osteocytes - cytology ; Pineal Gland - physiology ; pinealectomy ; stereology ; Tomography, X-Ray Computed ; vertebrae ; Vertebrates: endocrinology</subject><ispartof>Journal of pineal research, 2005-11, Vol.39 (4), p.392-399</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5313-da5bcdb3f2905c5fd182e0e3d2daae9c9b62d5cac4c2e4e012eda78a1da492403</citedby><cites>FETCH-LOGICAL-c5313-da5bcdb3f2905c5fd182e0e3d2daae9c9b62d5cac4c2e4e012eda78a1da492403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-079X.2005.00263.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-079X.2005.00263.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17163703$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16207295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Turgut, Mehmet</creatorcontrib><creatorcontrib>Kaplan, Süleyman</creatorcontrib><creatorcontrib>Turgut, Ahmet T.</creatorcontrib><creatorcontrib>Aslan, Hüseyin</creatorcontrib><creatorcontrib>Güvenç, Tolga</creatorcontrib><creatorcontrib>Çullu, Emre</creatorcontrib><creatorcontrib>Erdoǧan, Serpil</creatorcontrib><title>Morphological, stereological and radiological changes in pinealectomized chicken cervical vertebrae</title><title>Journal of pineal research</title><addtitle>J Pineal Res</addtitle><description>: Considerable evidence supports the hypothesis that melatonin plays an important role in osteogenesis. We carried out an experiment to investigate histological and radiological changes in the cervical vertebrae of pinealectomized chickens. Thirty new‐hatched chicks were divided into two groups: pinealectomized group (n = 15) and nonpinealectomized control (n = 15). Surgical pinealectomies were performed in Hybro broiler chickens at the age of 3 days. At 8 wk, one animal from each group was examined using computed tomography scanner to obtain density histograms of four consecutive vertebrae between the 4th and 7th cervical vertebral segments of chickens. At the end of the study, all animals were killed for subsequent measurement of bone mineral density (BMD) and macroscopic, radiographic and histopathological evaluation of specimens. We found that the profile of the density histogram in nonpinealectomized control group showed a spike profile for the vertebral body, indicating an increase in the amount of higher density tissues in this region, while a plateau‐shaped profile was obtained for the vertebral body in pinealectomy group, indicating the presence of heterogenous bone tissue. Accordingly, the mean value of BMD in pinealectomy group was significantly lower at the vertebral body in chickens compared with control subjects (P < 0.001). At the end of the experiment, almost all of the chickens in pinealectomy group developed a scoliotic curvature and the mean weight and length of the cervical vertebral bodies of the pinealectomized chickens were significantly lower than those of control group (P < 0.001). Although the numerical density of osteocytes and osteoblasts in pinealectomy group was significantly higher than that from the control group, total number of osteocytes but not osteoblasts in cervical vetrebrae from pinealectomized animals was significantly lower than that from nonpinealectomized control animals (P < 0.05). In conclusion, the results of the current study demonstrated for the first time pinealectomy‐induced histomorphometrical changes in chicken vertebral column using stereological methods, suggesting that pineal gland/melatonin may have an osteoinductive effect on bone formation, but further studies are needed to elucidate the relationship of our findings with some disorders such as postmenopausal and/or senile osteoporosis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Density</subject><subject>Cell Count</subject><subject>Cervical Vertebrae - anatomy & histology</subject><subject>Cervical Vertebrae - cytology</subject><subject>Cervical Vertebrae - diagnostic imaging</subject><subject>Chickens</subject><subject>computed tomography</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>melatonin</subject><subject>Organ Size</subject><subject>Osteoblasts - cytology</subject><subject>Osteocytes - cytology</subject><subject>Pineal Gland - physiology</subject><subject>pinealectomy</subject><subject>stereology</subject><subject>Tomography, X-Ray Computed</subject><subject>vertebrae</subject><subject>Vertebrates: endocrinology</subject><issn>0742-3098</issn><issn>1600-079X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9z0zAQxTUMHRoKX4HxBU7YrCRbtme4MBlaWkroAQZumrW0bpU6diolJeXTV27S9Ai66M_-nrR6j7GEQ8bj-DDPuAJIoax_ZwKgyACEktnmGZvsC8_ZBMpcpBLq6pC9DGEOAFVVqRfskCsBpaiLCTPfBr-8Grrh0hns3idhRZ4etwn2NvFo3f7AXGF_SSFxfbJ0PWFHZjUs3F-yseTMNfWJIX_7wN6SX1HjkV6xgxa7QK938xH7efz5x_RLev795HT66Tw1heQytVg0xjayFTUUpmgtrwQBSSssItWmbpSwhUGTG0E5ARdksayQW8xrkYM8Yu-29y79cLOmsNILFwx1HfY0rINWlVJqNOpfIC9zqWRZRLDagsYPIXhq9dK7Bfo7zUGPSei5Hg3Xo-F6TEI_JKE3Ufpm98a6WZB9Eu6sj8DbHYAh2tV67I0LT1zJYwsgI_dxy_1xHd39dwP67OI0LqI83cpdjHazl6O_1qqMf9S_Zif6mH-dzdT0QhfyHpl4teY</recordid><startdate>200511</startdate><enddate>200511</enddate><creator>Turgut, Mehmet</creator><creator>Kaplan, Süleyman</creator><creator>Turgut, Ahmet T.</creator><creator>Aslan, Hüseyin</creator><creator>Güvenç, Tolga</creator><creator>Çullu, Emre</creator><creator>Erdoǧan, Serpil</creator><general>Munksgaard International Publishers</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200511</creationdate><title>Morphological, stereological and radiological changes in pinealectomized chicken cervical vertebrae</title><author>Turgut, Mehmet ; Kaplan, Süleyman ; Turgut, Ahmet T. ; Aslan, Hüseyin ; Güvenç, Tolga ; Çullu, Emre ; Erdoǧan, Serpil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5313-da5bcdb3f2905c5fd182e0e3d2daae9c9b62d5cac4c2e4e012eda78a1da492403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Density</topic><topic>Cell Count</topic><topic>Cervical Vertebrae - anatomy & histology</topic><topic>Cervical Vertebrae - cytology</topic><topic>Cervical Vertebrae - diagnostic imaging</topic><topic>Chickens</topic><topic>computed tomography</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>melatonin</topic><topic>Organ Size</topic><topic>Osteoblasts - cytology</topic><topic>Osteocytes - cytology</topic><topic>Pineal Gland - physiology</topic><topic>pinealectomy</topic><topic>stereology</topic><topic>Tomography, X-Ray Computed</topic><topic>vertebrae</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Turgut, Mehmet</creatorcontrib><creatorcontrib>Kaplan, Süleyman</creatorcontrib><creatorcontrib>Turgut, Ahmet T.</creatorcontrib><creatorcontrib>Aslan, Hüseyin</creatorcontrib><creatorcontrib>Güvenç, Tolga</creatorcontrib><creatorcontrib>Çullu, Emre</creatorcontrib><creatorcontrib>Erdoǧan, Serpil</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pineal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Turgut, Mehmet</au><au>Kaplan, Süleyman</au><au>Turgut, Ahmet T.</au><au>Aslan, Hüseyin</au><au>Güvenç, Tolga</au><au>Çullu, Emre</au><au>Erdoǧan, Serpil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological, stereological and radiological changes in pinealectomized chicken cervical vertebrae</atitle><jtitle>Journal of pineal research</jtitle><addtitle>J Pineal Res</addtitle><date>2005-11</date><risdate>2005</risdate><volume>39</volume><issue>4</issue><spage>392</spage><epage>399</epage><pages>392-399</pages><issn>0742-3098</issn><eissn>1600-079X</eissn><coden>JPRSE9</coden><abstract>: Considerable evidence supports the hypothesis that melatonin plays an important role in osteogenesis. We carried out an experiment to investigate histological and radiological changes in the cervical vertebrae of pinealectomized chickens. Thirty new‐hatched chicks were divided into two groups: pinealectomized group (n = 15) and nonpinealectomized control (n = 15). Surgical pinealectomies were performed in Hybro broiler chickens at the age of 3 days. At 8 wk, one animal from each group was examined using computed tomography scanner to obtain density histograms of four consecutive vertebrae between the 4th and 7th cervical vertebral segments of chickens. At the end of the study, all animals were killed for subsequent measurement of bone mineral density (BMD) and macroscopic, radiographic and histopathological evaluation of specimens. We found that the profile of the density histogram in nonpinealectomized control group showed a spike profile for the vertebral body, indicating an increase in the amount of higher density tissues in this region, while a plateau‐shaped profile was obtained for the vertebral body in pinealectomy group, indicating the presence of heterogenous bone tissue. Accordingly, the mean value of BMD in pinealectomy group was significantly lower at the vertebral body in chickens compared with control subjects (P < 0.001). At the end of the experiment, almost all of the chickens in pinealectomy group developed a scoliotic curvature and the mean weight and length of the cervical vertebral bodies of the pinealectomized chickens were significantly lower than those of control group (P < 0.001). Although the numerical density of osteocytes and osteoblasts in pinealectomy group was significantly higher than that from the control group, total number of osteocytes but not osteoblasts in cervical vetrebrae from pinealectomized animals was significantly lower than that from nonpinealectomized control animals (P < 0.05). In conclusion, the results of the current study demonstrated for the first time pinealectomy‐induced histomorphometrical changes in chicken vertebral column using stereological methods, suggesting that pineal gland/melatonin may have an osteoinductive effect on bone formation, but further studies are needed to elucidate the relationship of our findings with some disorders such as postmenopausal and/or senile osteoporosis.</abstract><cop>Oxford, UK</cop><pub>Munksgaard International Publishers</pub><pmid>16207295</pmid><doi>10.1111/j.1600-079X.2005.00263.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Bone Density Cell Count Cervical Vertebrae - anatomy & histology Cervical Vertebrae - cytology Cervical Vertebrae - diagnostic imaging Chickens computed tomography Female Fundamental and applied biological sciences. Psychology Male melatonin Organ Size Osteoblasts - cytology Osteocytes - cytology Pineal Gland - physiology pinealectomy stereology Tomography, X-Ray Computed vertebrae Vertebrates: endocrinology |
| title | Morphological, stereological and radiological changes in pinealectomized chicken cervical vertebrae |
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