Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: Distribution of G/P types and finding of a rare G12 strain
Between May and August in 2003, a total of 251 fecal samples were collected from children and adults with diarrhea (5 inpatients and 246 outpatients) at a private hospital in the city of Ponta Grossa, the state of Paraná, Brazil. Group A rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77....
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| Veröffentlicht in: | Journal of medical virology 2006-09, Vol.78 (9), p.1241-1249 |
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| creator | Pietruchinski, Eduardo Benati, Fabrício Lauretti, Flávio Kisielius, Jonas Ueda, Marli Volotão, Eduardo M. Soares, Caroline C. Hoshino, Yasutaka Linhares, Rosa Elisa C. Nozawa, Carlos Santos, Norma |
| description | Between May and August in 2003, a total of 251 fecal samples were collected from children and adults with diarrhea (5 inpatients and 246 outpatients) at a private hospital in the city of Ponta Grossa, the state of Paraná, Brazil. Group A rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77.5%) from children under 5 years of age and 16 (22.5%) from individuals aged 6–72 years. All 71 strains exhibited a “long” RNA pattern when analyzed by PAGE. Sixty‐one positive samples that yielded enough RNA were submitted to PCR genotyping. The most frequent G/P genotype combination detected was G1P[8] (86.9%; 53/61) followed by G9P[8] (3.3%; 2/61) and G12P[9] (1.6%; 1/61). Rotaviruses with G2, G3, G4, P[4], or P[6] specificity were not detected. For three strains (4.9%) bearing G1 genotype, the VP4 specificity could no be determined, and two specimens (3.3%) remained G/P non‐typeable. One rotavirus strain (HC91) bearing G12P[9] genotype with a “long” electropherotype was isolated from an 11‐month‐old boy with diarrhea for the first time in Brazil. The cell‐culture grown HC91 strain was shown to belong to serotype G12 by neutralization. J. Med. Virol. 78:1241–1249, 2006. © 2006 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jmv.20686 |
| format | Article |
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Group A rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77.5%) from children under 5 years of age and 16 (22.5%) from individuals aged 6–72 years. All 71 strains exhibited a “long” RNA pattern when analyzed by PAGE. Sixty‐one positive samples that yielded enough RNA were submitted to PCR genotyping. The most frequent G/P genotype combination detected was G1P[8] (86.9%; 53/61) followed by G9P[8] (3.3%; 2/61) and G12P[9] (1.6%; 1/61). Rotaviruses with G2, G3, G4, P[4], or P[6] specificity were not detected. For three strains (4.9%) bearing G1 genotype, the VP4 specificity could no be determined, and two specimens (3.3%) remained G/P non‐typeable. One rotavirus strain (HC91) bearing G12P[9] genotype with a “long” electropherotype was isolated from an 11‐month‐old boy with diarrhea for the first time in Brazil. The cell‐culture grown HC91 strain was shown to belong to serotype G12 by neutralization. J. Med. Virol. 78:1241–1249, 2006. © 2006 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.20686</identifier><identifier>PMID: 16847962</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adolescent ; Adult ; Aged ; Biological and medical sciences ; Brazil - epidemiology ; Child ; Child, Preschool ; Data Collection ; Diarrhea - epidemiology ; Feces - virology ; Fundamental and applied biological sciences. Psychology ; G12 ; gastroenteritis ; genotyping ; Group a rotavirus ; Hospitals, Private ; Human viral diseases ; Humans ; Infant ; Infant, Newborn ; Infectious diseases ; Medical sciences ; Microbiology ; Middle Aged ; Miscellaneous ; Molecular Epidemiology ; Neutralization Tests ; Phylogeny ; RNA, Viral - genetics ; Rotavirus ; Rotavirus - classification ; Rotavirus - genetics ; Rotavirus - immunology ; Rotavirus - isolation & purification ; Rotavirus Infections - epidemiology ; Species Specificity ; Urban Population ; Viral diseases ; Virology</subject><ispartof>Journal of medical virology, 2006-09, Vol.78 (9), p.1241-1249</ispartof><rights>Copyright © 2006 Wiley‐Liss, Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4226-77a28d18a4d19bf5c346a2bc5430389562e622397eb387e92d1fbb197cb163843</citedby><cites>FETCH-LOGICAL-c4226-77a28d18a4d19bf5c346a2bc5430389562e622397eb387e92d1fbb197cb163843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.20686$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.20686$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18013213$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16847962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pietruchinski, Eduardo</creatorcontrib><creatorcontrib>Benati, Fabrício</creatorcontrib><creatorcontrib>Lauretti, Flávio</creatorcontrib><creatorcontrib>Kisielius, Jonas</creatorcontrib><creatorcontrib>Ueda, Marli</creatorcontrib><creatorcontrib>Volotão, Eduardo M.</creatorcontrib><creatorcontrib>Soares, Caroline C.</creatorcontrib><creatorcontrib>Hoshino, Yasutaka</creatorcontrib><creatorcontrib>Linhares, Rosa Elisa C.</creatorcontrib><creatorcontrib>Nozawa, Carlos</creatorcontrib><creatorcontrib>Santos, Norma</creatorcontrib><title>Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: Distribution of G/P types and finding of a rare G12 strain</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>Between May and August in 2003, a total of 251 fecal samples were collected from children and adults with diarrhea (5 inpatients and 246 outpatients) at a private hospital in the city of Ponta Grossa, the state of Paraná, Brazil. Group A rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77.5%) from children under 5 years of age and 16 (22.5%) from individuals aged 6–72 years. All 71 strains exhibited a “long” RNA pattern when analyzed by PAGE. Sixty‐one positive samples that yielded enough RNA were submitted to PCR genotyping. The most frequent G/P genotype combination detected was G1P[8] (86.9%; 53/61) followed by G9P[8] (3.3%; 2/61) and G12P[9] (1.6%; 1/61). Rotaviruses with G2, G3, G4, P[4], or P[6] specificity were not detected. For three strains (4.9%) bearing G1 genotype, the VP4 specificity could no be determined, and two specimens (3.3%) remained G/P non‐typeable. One rotavirus strain (HC91) bearing G12P[9] genotype with a “long” electropherotype was isolated from an 11‐month‐old boy with diarrhea for the first time in Brazil. The cell‐culture grown HC91 strain was shown to belong to serotype G12 by neutralization. J. Med. Virol. 78:1241–1249, 2006. © 2006 Wiley‐Liss, Inc.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Brazil - epidemiology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Data Collection</subject><subject>Diarrhea - epidemiology</subject><subject>Feces - virology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>G12</subject><subject>gastroenteritis</subject><subject>genotyping</subject><subject>Group a rotavirus</subject><subject>Hospitals, Private</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Molecular Epidemiology</subject><subject>Neutralization Tests</subject><subject>Phylogeny</subject><subject>RNA, Viral - genetics</subject><subject>Rotavirus</subject><subject>Rotavirus - classification</subject><subject>Rotavirus - genetics</subject><subject>Rotavirus - immunology</subject><subject>Rotavirus - isolation & purification</subject><subject>Rotavirus Infections - epidemiology</subject><subject>Species Specificity</subject><subject>Urban Population</subject><subject>Viral diseases</subject><subject>Virology</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS1ERYfCghdA3oDEIh3_xYnZQYEpowIVPzNL68Z2GLeZZLCTwvAIfeo6nYGuECtLPt85R7oHoSeUHFNC2PRifXXMiCzlPTShRMlMkYLeRxNChcykpPkhehjjBSGkVIw9QIdUlqJQkk3Q9eeuhysfhoithxBWDrBvsVn5xgbXYmgtBjs0fRy_Acdu6FcuJML3W9zV-HWA374ZRUYIf4nf-NgHXw2979pRn03Pcb_duHgbVfvW-vb7KAAOEByeUYaTA3z7CB3U0ET3eP8eoW_v3n49Oc3OPs3en7w6y4xgTGZFAay0tARhqarq3HAhgVUmF5zwUuWSOckYV4WreFk4xSytq4qqwlRU8lLwI_R8l7sJ3Y_BxV6vfTSuaaB13RB1uqMU6Tj_BRnJheSSJ_DFDjShizG4Wm-CX0PYakr0uJBOC-nbhRL7dB86VGtn78j9JAl4tgcgGmjqAK3x8Y4rCeWMjqXTHffTN27770Y9_7D4U53tHGki9-uvA8KllgUvcr38ONOL5Xx-vlx80ZzfADzItSA</recordid><startdate>200609</startdate><enddate>200609</enddate><creator>Pietruchinski, Eduardo</creator><creator>Benati, Fabrício</creator><creator>Lauretti, Flávio</creator><creator>Kisielius, Jonas</creator><creator>Ueda, Marli</creator><creator>Volotão, Eduardo M.</creator><creator>Soares, Caroline C.</creator><creator>Hoshino, Yasutaka</creator><creator>Linhares, Rosa Elisa C.</creator><creator>Nozawa, Carlos</creator><creator>Santos, Norma</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200609</creationdate><title>Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: Distribution of G/P types and finding of a rare G12 strain</title><author>Pietruchinski, Eduardo ; Benati, Fabrício ; Lauretti, Flávio ; Kisielius, Jonas ; Ueda, Marli ; Volotão, Eduardo M. ; Soares, Caroline C. ; Hoshino, Yasutaka ; Linhares, Rosa Elisa C. ; Nozawa, Carlos ; Santos, Norma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4226-77a28d18a4d19bf5c346a2bc5430389562e622397eb387e92d1fbb197cb163843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Brazil - epidemiology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Data Collection</topic><topic>Diarrhea - epidemiology</topic><topic>Feces - virology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>G12</topic><topic>gastroenteritis</topic><topic>genotyping</topic><topic>Group a rotavirus</topic><topic>Hospitals, Private</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Molecular Epidemiology</topic><topic>Neutralization Tests</topic><topic>Phylogeny</topic><topic>RNA, Viral - genetics</topic><topic>Rotavirus</topic><topic>Rotavirus - classification</topic><topic>Rotavirus - genetics</topic><topic>Rotavirus - immunology</topic><topic>Rotavirus - isolation & purification</topic><topic>Rotavirus Infections - epidemiology</topic><topic>Species Specificity</topic><topic>Urban Population</topic><topic>Viral diseases</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pietruchinski, Eduardo</creatorcontrib><creatorcontrib>Benati, Fabrício</creatorcontrib><creatorcontrib>Lauretti, Flávio</creatorcontrib><creatorcontrib>Kisielius, Jonas</creatorcontrib><creatorcontrib>Ueda, Marli</creatorcontrib><creatorcontrib>Volotão, Eduardo M.</creatorcontrib><creatorcontrib>Soares, Caroline C.</creatorcontrib><creatorcontrib>Hoshino, Yasutaka</creatorcontrib><creatorcontrib>Linhares, Rosa Elisa C.</creatorcontrib><creatorcontrib>Nozawa, Carlos</creatorcontrib><creatorcontrib>Santos, Norma</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pietruchinski, Eduardo</au><au>Benati, Fabrício</au><au>Lauretti, Flávio</au><au>Kisielius, Jonas</au><au>Ueda, Marli</au><au>Volotão, Eduardo M.</au><au>Soares, Caroline C.</au><au>Hoshino, Yasutaka</au><au>Linhares, Rosa Elisa C.</au><au>Nozawa, Carlos</au><au>Santos, Norma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: Distribution of G/P types and finding of a rare G12 strain</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>2006-09</date><risdate>2006</risdate><volume>78</volume><issue>9</issue><spage>1241</spage><epage>1249</epage><pages>1241-1249</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>Between May and August in 2003, a total of 251 fecal samples were collected from children and adults with diarrhea (5 inpatients and 246 outpatients) at a private hospital in the city of Ponta Grossa, the state of Paraná, Brazil. Group A rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77.5%) from children under 5 years of age and 16 (22.5%) from individuals aged 6–72 years. All 71 strains exhibited a “long” RNA pattern when analyzed by PAGE. Sixty‐one positive samples that yielded enough RNA were submitted to PCR genotyping. The most frequent G/P genotype combination detected was G1P[8] (86.9%; 53/61) followed by G9P[8] (3.3%; 2/61) and G12P[9] (1.6%; 1/61). Rotaviruses with G2, G3, G4, P[4], or P[6] specificity were not detected. For three strains (4.9%) bearing G1 genotype, the VP4 specificity could no be determined, and two specimens (3.3%) remained G/P non‐typeable. One rotavirus strain (HC91) bearing G12P[9] genotype with a “long” electropherotype was isolated from an 11‐month‐old boy with diarrhea for the first time in Brazil. The cell‐culture grown HC91 strain was shown to belong to serotype G12 by neutralization. J. Med. Virol. 78:1241–1249, 2006. © 2006 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16847962</pmid><doi>10.1002/jmv.20686</doi><tpages>9</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Biological and medical sciences Brazil - epidemiology Child Child, Preschool Data Collection Diarrhea - epidemiology Feces - virology Fundamental and applied biological sciences. Psychology G12 gastroenteritis genotyping Group a rotavirus Hospitals, Private Human viral diseases Humans Infant Infant, Newborn Infectious diseases Medical sciences Microbiology Middle Aged Miscellaneous Molecular Epidemiology Neutralization Tests Phylogeny RNA, Viral - genetics Rotavirus Rotavirus - classification Rotavirus - genetics Rotavirus - immunology Rotavirus - isolation & purification Rotavirus Infections - epidemiology Species Specificity Urban Population Viral diseases Virology |
| title | Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: Distribution of G/P types and finding of a rare G12 strain |
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