Sequencing of the intergenic 16S-23S rRNA spacer (ITS) region of Mollicutes species and their identification using microarray-based assay and DNA sequencing

We have completed sequencing the 16S-23S rRNA intergenic transcribed spacer (ITS) region of most known Mycoplasma , Acholeplasma , Ureaplasma , Mesoplasma , and Spiroplasma species. Analysis of the sequence data revealed a significant interspecies variability and low intraspecies polymorphism of the...

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Veröffentlicht in:	Applied microbiology and biotechnology 2006-08, Vol.71 (5), p.680-698
Hauptverfasser:	VOLOKHOV, Dmitriy V, GEORGE, Joseph, LIU, Sue X, IKONOMI, Pranvera, ANDERSON, Christine, CHIZHIKOV, Vladimir
Format:	Artikel
Sprache:	eng
Schlagworte:	Acholeplasma Animals Bacterial Typing Techniques Biological and medical sciences Biotechnology Contaminants DNA, Bacterial - analysis DNA, Ribosomal Spacer - analysis Fundamental and applied biological sciences. Psychology Genes Humans Molecular Sequence Data Mollicutes Mycoplasma Oligonucleotide Array Sequence Analysis - methods Polymerase Chain Reaction - methods RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics Sensitivity and Specificity Sequence Analysis, DNA - methods Species Specificity Spiroplasma Tenericutes - classification Tenericutes - genetics Ureaplasma
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