Nitric oxide-dependent activation of pig oocytes: Role of calcium
Pig oocytes matured in vitro are parthenogenetically activated after treatment with nitric oxide (NO)-donor SNAP. The chelation of intracellular calcium ions with BAPTA-AM suppressed the SNAP-induced activation in a dose-dependent manner. Activation by a NO-donor is dependent on the influx of calciu...
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| Veröffentlicht in: | Molecular and cellular endocrinology 2005-10, Vol.242 (1), p.16-22 |
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| container_title | Molecular and cellular endocrinology |
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| creator | Petr, Jaroslav Rajmon, Radko Lánská, Vilma Sedmíková, Markéta Jílek, František |
| description | Pig oocytes matured in vitro are parthenogenetically activated after treatment with nitric oxide (NO)-donor SNAP. The chelation of intracellular calcium ions with BAPTA-AM suppressed the SNAP-induced activation in a dose-dependent manner. Activation by a NO-donor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil had significantly reduced the activation rate in SNAP-treated oocytes. The blockage of inositol triphosphate receptors had no effect on the activation of oocytes by a NO-donor. On the other hand, the blockers of ryanodine receptors, procaine and ruthenium red, inhibited the activation of oocytes induced by a NO-donor.
These data indicate that the activation of pig oocytes by a NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular spaces. For the mobilization of intracellular calcium stores, it is the ryanodine receptors and not the inositol triphosphate receptors that play a key role. |
| doi_str_mv | 10.1016/j.mce.2005.05.004 |
| format | Article |
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These data indicate that the activation of pig oocytes by a NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular spaces. For the mobilization of intracellular calcium stores, it is the ryanodine receptors and not the inositol triphosphate receptors that play a key role.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/j.mce.2005.05.004</identifier><identifier>PMID: 15967570</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Calcium ; Calcium - metabolism ; Egtazic Acid - analogs & derivatives ; Egtazic Acid - pharmacology ; Female ; Heparin - pharmacology ; Macrocyclic Compounds ; Nitric oxide ; Nitric Oxide - metabolism ; Oocyte ; Oocytes - drug effects ; Oocytes - metabolism ; Oxazoles - pharmacology ; Parthenogenetic activation ; Pig ; Procaine - pharmacology ; Ruthenium Red - pharmacology ; Swine ; Verapamil - pharmacology</subject><ispartof>Molecular and cellular endocrinology, 2005-10, Vol.242 (1), p.16-22</ispartof><rights>2005 Elsevier Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-b26d3bb5fe0ebd0bd55772da32b1024df9b1bc42bf9674b3b26c81cc8c6bb3ea3</citedby><cites>FETCH-LOGICAL-c351t-b26d3bb5fe0ebd0bd55772da32b1024df9b1bc42bf9674b3b26c81cc8c6bb3ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mce.2005.05.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15967570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Petr, Jaroslav</creatorcontrib><creatorcontrib>Rajmon, Radko</creatorcontrib><creatorcontrib>Lánská, Vilma</creatorcontrib><creatorcontrib>Sedmíková, Markéta</creatorcontrib><creatorcontrib>Jílek, František</creatorcontrib><title>Nitric oxide-dependent activation of pig oocytes: Role of calcium</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Pig oocytes matured in vitro are parthenogenetically activated after treatment with nitric oxide (NO)-donor SNAP. The chelation of intracellular calcium ions with BAPTA-AM suppressed the SNAP-induced activation in a dose-dependent manner. Activation by a NO-donor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil had significantly reduced the activation rate in SNAP-treated oocytes. The blockage of inositol triphosphate receptors had no effect on the activation of oocytes by a NO-donor. On the other hand, the blockers of ryanodine receptors, procaine and ruthenium red, inhibited the activation of oocytes induced by a NO-donor.
These data indicate that the activation of pig oocytes by a NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular spaces. For the mobilization of intracellular calcium stores, it is the ryanodine receptors and not the inositol triphosphate receptors that play a key role.</description><subject>Animals</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Egtazic Acid - analogs & derivatives</subject><subject>Egtazic Acid - pharmacology</subject><subject>Female</subject><subject>Heparin - pharmacology</subject><subject>Macrocyclic Compounds</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - metabolism</subject><subject>Oocyte</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - metabolism</subject><subject>Oxazoles - pharmacology</subject><subject>Parthenogenetic activation</subject><subject>Pig</subject><subject>Procaine - pharmacology</subject><subject>Ruthenium Red - pharmacology</subject><subject>Swine</subject><subject>Verapamil - pharmacology</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9Lw0AQxRdRbK1-AC-Sk7fU2U02m-pJiv-gKIiel-zsRLYk2ZhNi_32JrTgTXgwMPzeY-YxdslhzoFnN-t5jTQXAHI-CtIjNuW5EnEOUh2zKSSQxEqAmrCzENYAoKTIT9mEy0WmpIIpu391fecw8j_OUmyppcZS00cF9m5b9M43kS-j1n1F3uOup3AbvfuKxiUWFbpNfc5OyqIKdHGYM_b5-PCxfI5Xb08vy_tVjInkfWxEZhNjZElAxoKxUiolbJEIw0GktlwYbjAVphxOS00y8JhzxBwzYxIqkhm73ue2nf_eUOh17QJSVRUN-U3QWZ7JLFNqAPkexM6H0FGp287VRbfTHPTYm17roTc99qZHQTp4rg7hG1OT_XMcihqAuz1Aw4tbR50O6KhBsq4j7LX17p_4X6wkfko</recordid><startdate>20051020</startdate><enddate>20051020</enddate><creator>Petr, Jaroslav</creator><creator>Rajmon, Radko</creator><creator>Lánská, Vilma</creator><creator>Sedmíková, Markéta</creator><creator>Jílek, František</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051020</creationdate><title>Nitric oxide-dependent activation of pig oocytes: Role of calcium</title><author>Petr, Jaroslav ; Rajmon, Radko ; Lánská, Vilma ; Sedmíková, Markéta ; Jílek, František</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-b26d3bb5fe0ebd0bd55772da32b1024df9b1bc42bf9674b3b26c81cc8c6bb3ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Egtazic Acid - analogs & derivatives</topic><topic>Egtazic Acid - pharmacology</topic><topic>Female</topic><topic>Heparin - pharmacology</topic><topic>Macrocyclic Compounds</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - metabolism</topic><topic>Oocyte</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - metabolism</topic><topic>Oxazoles - pharmacology</topic><topic>Parthenogenetic activation</topic><topic>Pig</topic><topic>Procaine - pharmacology</topic><topic>Ruthenium Red - pharmacology</topic><topic>Swine</topic><topic>Verapamil - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petr, Jaroslav</creatorcontrib><creatorcontrib>Rajmon, Radko</creatorcontrib><creatorcontrib>Lánská, Vilma</creatorcontrib><creatorcontrib>Sedmíková, Markéta</creatorcontrib><creatorcontrib>Jílek, František</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petr, Jaroslav</au><au>Rajmon, Radko</au><au>Lánská, Vilma</au><au>Sedmíková, Markéta</au><au>Jílek, František</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric oxide-dependent activation of pig oocytes: Role of calcium</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2005-10-20</date><risdate>2005</risdate><volume>242</volume><issue>1</issue><spage>16</spage><epage>22</epage><pages>16-22</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>Pig oocytes matured in vitro are parthenogenetically activated after treatment with nitric oxide (NO)-donor SNAP. The chelation of intracellular calcium ions with BAPTA-AM suppressed the SNAP-induced activation in a dose-dependent manner. Activation by a NO-donor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil had significantly reduced the activation rate in SNAP-treated oocytes. The blockage of inositol triphosphate receptors had no effect on the activation of oocytes by a NO-donor. On the other hand, the blockers of ryanodine receptors, procaine and ruthenium red, inhibited the activation of oocytes induced by a NO-donor.
These data indicate that the activation of pig oocytes by a NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular spaces. For the mobilization of intracellular calcium stores, it is the ryanodine receptors and not the inositol triphosphate receptors that play a key role.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>15967570</pmid><doi>10.1016/j.mce.2005.05.004</doi><tpages>7</tpages></addata></record> |
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| ispartof | Molecular and cellular endocrinology, 2005-10, Vol.242 (1), p.16-22 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Calcium Calcium - metabolism Egtazic Acid - analogs & derivatives Egtazic Acid - pharmacology Female Heparin - pharmacology Macrocyclic Compounds Nitric oxide Nitric Oxide - metabolism Oocyte Oocytes - drug effects Oocytes - metabolism Oxazoles - pharmacology Parthenogenetic activation Pig Procaine - pharmacology Ruthenium Red - pharmacology Swine Verapamil - pharmacology |
| title | Nitric oxide-dependent activation of pig oocytes: Role of calcium |
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