Endothelial progenitor cell sprouting in spheroid cultures is resistant to inhibition by osteoblasts: A model for bone replacement grafts
Survival of tissue transplants generated in vitro is strongly limited by the slow process of graft vascularization in vivo. A method to enhance graft vascularization is to establish a primitive vascular plexus within the graft prior to transplantation. Endothelial cells (EC) cultured as multicellula...
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| Veröffentlicht in: | FEBS letters 2005-10, Vol.579 (24), p.5338-5342 |
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| container_issue | 24 |
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| container_title | FEBS letters |
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| creator | Stahl, Andreas Wu, Xiao Wenger, Andreas Klagsbrun, Michael Kurschat, Peter |
| description | Survival of tissue transplants generated in vitro is strongly limited by the slow process of graft vascularization in vivo. A method to enhance graft vascularization is to establish a primitive vascular plexus within the graft prior to transplantation. Endothelial cells (EC) cultured as multicellular spheroids within a collagen matrix form sprouts resembling angiogenesis in vitro. However, osteoblasts integrated into the graft suppress EC sprouting. This inhibition depends on direct cell–cell-interactions and is characteristic of mature ECs isolated from preexisting vessels. In contrast, sprouting of human blood endothelial progenitor cells is not inhibited by osteoblasts, making these cells suitable for tissue engineering of pre-vascularized bone grafts. |
| doi_str_mv | 10.1016/j.febslet.2005.09.005 |
| format | Article |
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| ispartof | FEBS letters, 2005-10, Vol.579 (24), p.5338-5342 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Journals; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Angiogenesis Bone Substitutes Cells, Cultured Endothelial progenitor cells Endothelium, Vascular - cytology Humans Osteoblasts Osteoblasts - cytology Spheroid culture Sprouting Stem Cells - cytology Tissue engineering |
| title | Endothelial progenitor cell sprouting in spheroid cultures is resistant to inhibition by osteoblasts: A model for bone replacement grafts |
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