C. elegans TCF protein, POP-1, converts from repressor to activator as a result of Wnt-induced lowering of nuclear levels
Canonical Wnt signaling converts the TCF/LEF transcription factor from repressor to activator by increasing nuclear levels of its coactivator, β-catenin. A striking exception had been reported for Wnt-induced endoderm formation during C. elegans embryogenesis. It has long been believed that transcri...
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| Veröffentlicht in: | Developmental biology 2005-09, Vol.285 (2), p.584-592 |
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| creator | Shetty, Premnath Lo, Miao-Chia Robertson, Scott M. Lin, Rueyling |
| description | Canonical Wnt signaling converts the TCF/LEF transcription factor from repressor to activator by increasing nuclear levels of its coactivator, β-catenin. A striking exception had been reported for Wnt-induced endoderm formation during
C. elegans embryogenesis. It has long been believed that transcriptional activation of Wnt target genes in the endoderm precursor occurred due to a lowering of nuclear levels of the worm TCF/LEF protein, POP-1, effectively alleviating POP-1 repressive activity. Contrary to this model, we demonstrate here that POP-1 directly activates Wnt target genes in the endoderm precursor. Wnt converts POP-1 from a repressor to an activator, and this conversion requires that POP-1 nuclear levels be lowered in the endoderm precursor. We propose that the balance between TCF/LEF and coactivator(s), achieved by elevating coactivator levels (the canonical pathway) and/or reducing TCF/LEF levels (worm endoderm), determines Wnt signal strength. |
| doi_str_mv | 10.1016/j.ydbio.2005.07.008 |
| format | Article |
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We propose that the balance between TCF/LEF and coactivator(s), achieved by elevating coactivator levels (the canonical pathway) and/or reducing TCF/LEF levels (worm endoderm), determines Wnt signal strength.</description><subject>Animals</subject><subject>C. elegans</subject><subject>Caenorhabditis elegans - embryology</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans Proteins - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Endoderm</subject><subject>Endoderm - physiology</subject><subject>Gene Expression Regulation - physiology</subject><subject>Genetic Markers - genetics</subject><subject>High Mobility Group Proteins - metabolism</subject><subject>Microscopy, Fluorescence</subject><subject>Plasmids - genetics</subject><subject>POP-1</subject><subject>Signal Transduction - genetics</subject><subject>Signal Transduction - physiology</subject><subject>TCF/LEF</subject><subject>Wnt</subject><subject>Wnt Proteins - metabolism</subject><subject>β-catenin</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEGP0zAQhS0EYsvCL0BCPnHahBk7cdwDB1SxgLTS7mER3CzHnqxcpXGxnaL-e1JaiRun0cy8N6P3MfYWoUZA9WFbH30fYi0A2hq6GkA_YyuEdVu1qvn5nK0AUFSoQF2xVzlvAUBqLV-yK1SIAkGu2HFTcxrpyU6ZP25u-T7FQmG64Q_3DxXecBenA6WS-ZDijifaJ8o5Jl4it66Egy1LYzO3yy7PY-Fx4D-mUoXJz448H-NvSmF6Os2n2Y1kEx_pQGN-zV4Mdsz05lKv2ffbz4-br9Xd_Zdvm093lWuELJX3AjvXDw1aLQZlEfpmrXstHHTCds7bTpPUXrddM_QaUfbNINaubzslWyvkNXt_vrtE-zVTLmYXsqNxtBPFORulVYsS9SKUZ6FLMedEg9mnsLPpaBDMibjZmr_EzYm4gc4sxBfXu8v5ud-R_-e5IF4EH8-CJTMdAiWTXaBpgRMSuWJ8DP998AcXaJMj</recordid><startdate>20050915</startdate><enddate>20050915</enddate><creator>Shetty, Premnath</creator><creator>Lo, Miao-Chia</creator><creator>Robertson, Scott M.</creator><creator>Lin, Rueyling</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050915</creationdate><title>C. elegans TCF protein, POP-1, converts from repressor to activator as a result of Wnt-induced lowering of nuclear levels</title><author>Shetty, Premnath ; Lo, Miao-Chia ; Robertson, Scott M. ; Lin, Rueyling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-dd217cbf41a82f6a10b498b82c072a7cda78e38d8574fb8113b4f29cb57635a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>C. elegans</topic><topic>Caenorhabditis elegans - embryology</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans Proteins - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Endoderm</topic><topic>Endoderm - physiology</topic><topic>Gene Expression Regulation - physiology</topic><topic>Genetic Markers - genetics</topic><topic>High Mobility Group Proteins - metabolism</topic><topic>Microscopy, Fluorescence</topic><topic>Plasmids - genetics</topic><topic>POP-1</topic><topic>Signal Transduction - genetics</topic><topic>Signal Transduction - physiology</topic><topic>TCF/LEF</topic><topic>Wnt</topic><topic>Wnt Proteins - metabolism</topic><topic>β-catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shetty, Premnath</creatorcontrib><creatorcontrib>Lo, Miao-Chia</creatorcontrib><creatorcontrib>Robertson, Scott M.</creatorcontrib><creatorcontrib>Lin, Rueyling</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shetty, Premnath</au><au>Lo, Miao-Chia</au><au>Robertson, Scott M.</au><au>Lin, Rueyling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C. elegans TCF protein, POP-1, converts from repressor to activator as a result of Wnt-induced lowering of nuclear levels</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2005-09-15</date><risdate>2005</risdate><volume>285</volume><issue>2</issue><spage>584</spage><epage>592</epage><pages>584-592</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Canonical Wnt signaling converts the TCF/LEF transcription factor from repressor to activator by increasing nuclear levels of its coactivator, β-catenin. A striking exception had been reported for Wnt-induced endoderm formation during
C. elegans embryogenesis. It has long been believed that transcriptional activation of Wnt target genes in the endoderm precursor occurred due to a lowering of nuclear levels of the worm TCF/LEF protein, POP-1, effectively alleviating POP-1 repressive activity. Contrary to this model, we demonstrate here that POP-1 directly activates Wnt target genes in the endoderm precursor. Wnt converts POP-1 from a repressor to an activator, and this conversion requires that POP-1 nuclear levels be lowered in the endoderm precursor. We propose that the balance between TCF/LEF and coactivator(s), achieved by elevating coactivator levels (the canonical pathway) and/or reducing TCF/LEF levels (worm endoderm), determines Wnt signal strength.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16112103</pmid><doi>10.1016/j.ydbio.2005.07.008</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Developmental biology, 2005-09, Vol.285 (2), p.584-592 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals C. elegans Caenorhabditis elegans - embryology Caenorhabditis elegans - genetics Caenorhabditis elegans Proteins - metabolism DNA-Binding Proteins - metabolism Endoderm Endoderm - physiology Gene Expression Regulation - physiology Genetic Markers - genetics High Mobility Group Proteins - metabolism Microscopy, Fluorescence Plasmids - genetics POP-1 Signal Transduction - genetics Signal Transduction - physiology TCF/LEF Wnt Wnt Proteins - metabolism β-catenin |
| title | C. elegans TCF protein, POP-1, converts from repressor to activator as a result of Wnt-induced lowering of nuclear levels |
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