Optimization of the Extraction of Antioxidants from Dunaliella salina Microalga by Pressurized Liquids
In this work, extraction of antioxidant compounds from Dunaliella salina microalga is optimized by combining pressurized liquid extraction (PLE) and experimental design (three-level factorial design) with three different solvents (hexane, ethanol, and water). Two main factors were considered, the ex...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2006-07, Vol.54 (15), p.5597-5603 |
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| creator | Herrero, Miguel Jaime, Laura Martín-Álvarez, Pedro J Cifuentes, Alejandro Ibáñez, Elena |
| description | In this work, extraction of antioxidant compounds from Dunaliella salina microalga is optimized by combining pressurized liquid extraction (PLE) and experimental design (three-level factorial design) with three different solvents (hexane, ethanol, and water). Two main factors were considered, the extraction temperature (40, 100, and 160 °C) and the extraction time (5, 17.5, and 30 min). As response variables, the extraction yield (percent dry weight/initial weight) and the antioxidant activity of the extracts (determined using the TEAC method) were used. The parameters of the model were estimated by multiple linear regression. Results showed that the extraction temperature was the factor having the strongest influence (positive) on the two response variables. The best yields were obtained with ethanol at the higher extraction temperature and time tested. Besides, although hexane extracts provided the best antioxidant activity, ethanol extracts were also very active. The chemical characterization of ethanol extracts was carried out using HPLC-DAD, and attempts have been made to correlate their chemical composition with the antioxidant activity measured. Results pointed out that the extracts contained, besides all-trans-β-carotene and isomers, several different minor carotenoids that seemed to make a contribution to the antioxidant activity of the extracts. Keywords: PLE; Dunaliella salina; TEAC; antioxidant compounds; optimization |
| doi_str_mv | 10.1021/jf060546q |
| format | Article |
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Two main factors were considered, the extraction temperature (40, 100, and 160 °C) and the extraction time (5, 17.5, and 30 min). As response variables, the extraction yield (percent dry weight/initial weight) and the antioxidant activity of the extracts (determined using the TEAC method) were used. The parameters of the model were estimated by multiple linear regression. Results showed that the extraction temperature was the factor having the strongest influence (positive) on the two response variables. The best yields were obtained with ethanol at the higher extraction temperature and time tested. Besides, although hexane extracts provided the best antioxidant activity, ethanol extracts were also very active. The chemical characterization of ethanol extracts was carried out using HPLC-DAD, and attempts have been made to correlate their chemical composition with the antioxidant activity measured. Results pointed out that the extracts contained, besides all-trans-β-carotene and isomers, several different minor carotenoids that seemed to make a contribution to the antioxidant activity of the extracts. Keywords: PLE; Dunaliella salina; TEAC; antioxidant compounds; optimization</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf060546q</identifier><identifier>PMID: 16848551</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>antioxidant activity ; antioxidants ; Antioxidants - isolation & purification ; Biological and medical sciences ; carotenoids ; Chlorophyta - chemistry ; Chromatography, High Pressure Liquid ; Dunaliella salina ; ethanol ; extraction ; Food engineering ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; hexane ; Linear Models ; Pressure ; pressure treatment ; pressurized liquid extraction ; Solutions ; Solvents ; Temperature ; Time Factors ; water</subject><ispartof>Journal of agricultural and food chemistry, 2006-07, Vol.54 (15), p.5597-5603</ispartof><rights>Copyright © 2006 American Chemical Society</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a537t-4e8c6b79821f16d6851fb801a765fc1a5f347158758d373c93dcf6e1d3e640cc3</citedby><cites>FETCH-LOGICAL-a537t-4e8c6b79821f16d6851fb801a765fc1a5f347158758d373c93dcf6e1d3e640cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf060546q$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf060546q$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17972199$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16848551$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Herrero, Miguel</creatorcontrib><creatorcontrib>Jaime, Laura</creatorcontrib><creatorcontrib>Martín-Álvarez, Pedro J</creatorcontrib><creatorcontrib>Cifuentes, Alejandro</creatorcontrib><creatorcontrib>Ibáñez, Elena</creatorcontrib><title>Optimization of the Extraction of Antioxidants from Dunaliella salina Microalga by Pressurized Liquids</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>In this work, extraction of antioxidant compounds from Dunaliella salina microalga is optimized by combining pressurized liquid extraction (PLE) and experimental design (three-level factorial design) with three different solvents (hexane, ethanol, and water). Two main factors were considered, the extraction temperature (40, 100, and 160 °C) and the extraction time (5, 17.5, and 30 min). As response variables, the extraction yield (percent dry weight/initial weight) and the antioxidant activity of the extracts (determined using the TEAC method) were used. The parameters of the model were estimated by multiple linear regression. Results showed that the extraction temperature was the factor having the strongest influence (positive) on the two response variables. The best yields were obtained with ethanol at the higher extraction temperature and time tested. Besides, although hexane extracts provided the best antioxidant activity, ethanol extracts were also very active. The chemical characterization of ethanol extracts was carried out using HPLC-DAD, and attempts have been made to correlate their chemical composition with the antioxidant activity measured. Results pointed out that the extracts contained, besides all-trans-β-carotene and isomers, several different minor carotenoids that seemed to make a contribution to the antioxidant activity of the extracts. Keywords: PLE; Dunaliella salina; TEAC; antioxidant compounds; optimization</description><subject>antioxidant activity</subject><subject>antioxidants</subject><subject>Antioxidants - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>carotenoids</subject><subject>Chlorophyta - chemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dunaliella salina</subject><subject>ethanol</subject><subject>extraction</subject><subject>Food engineering</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>hexane</subject><subject>Linear Models</subject><subject>Pressure</subject><subject>pressure treatment</subject><subject>pressurized liquid extraction</subject><subject>Solutions</subject><subject>Solvents</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>water</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UtvEzEQAGALgWgoHPgD4EuROCx41uvHHqtQoCKolZpeuFgTr10cNruJvSul_fU4StpckDjZ8nwaz4OQt8A-ASvh89IzyUQlN8_IBETJCgGgn5MJy8FCCwkn5FVKS8aYFoq9JCcgdaWFgAnxV-shrMIDDqHvaO_p8NvRi-0Q0T6-nHf5tg0NdkOiPvYr-mXssA2ubZGmfOmQ_gw29tjeIV3c0-voUhpjeHANnYXNGJr0mrzw2Cb35nCektuvF_Pp92J29e1yej4rUHA1FJXTVi5UrUvwIBupBfiFZoBKCm8BheeVAqGV0A1X3Na8sV46aLiTFbOWn5IP-7zr2G9GlwazCsnuKu1cPyYjtRRQSvZfCHVZg6p38OMe5gZTis6bdQwrjPcGmNlN3zxNP9t3h6TjYuWaozyMO4OzA8BksfUROxvS0alalVDX2RV7F9Lgtk9xjH-MVFwJM7--MXz6i8_Vj1xF9u_33mNv8C7mnLc3JQPOgNU87_34M9pklv0Y8wbTP1r4C7dKsOo</recordid><startdate>20060726</startdate><enddate>20060726</enddate><creator>Herrero, Miguel</creator><creator>Jaime, Laura</creator><creator>Martín-Álvarez, Pedro J</creator><creator>Cifuentes, Alejandro</creator><creator>Ibáñez, Elena</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060726</creationdate><title>Optimization of the Extraction of Antioxidants from Dunaliella salina Microalga by Pressurized Liquids</title><author>Herrero, Miguel ; Jaime, Laura ; Martín-Álvarez, Pedro J ; Cifuentes, Alejandro ; Ibáñez, Elena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a537t-4e8c6b79821f16d6851fb801a765fc1a5f347158758d373c93dcf6e1d3e640cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>antioxidant activity</topic><topic>antioxidants</topic><topic>Antioxidants - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>carotenoids</topic><topic>Chlorophyta - chemistry</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dunaliella salina</topic><topic>ethanol</topic><topic>extraction</topic><topic>Food engineering</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>hexane</topic><topic>Linear Models</topic><topic>Pressure</topic><topic>pressure treatment</topic><topic>pressurized liquid extraction</topic><topic>Solutions</topic><topic>Solvents</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herrero, Miguel</creatorcontrib><creatorcontrib>Jaime, Laura</creatorcontrib><creatorcontrib>Martín-Álvarez, Pedro J</creatorcontrib><creatorcontrib>Cifuentes, Alejandro</creatorcontrib><creatorcontrib>Ibáñez, Elena</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herrero, Miguel</au><au>Jaime, Laura</au><au>Martín-Álvarez, Pedro J</au><au>Cifuentes, Alejandro</au><au>Ibáñez, Elena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of the Extraction of Antioxidants from Dunaliella salina Microalga by Pressurized Liquids</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2006-07-26</date><risdate>2006</risdate><volume>54</volume><issue>15</issue><spage>5597</spage><epage>5603</epage><pages>5597-5603</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>In this work, extraction of antioxidant compounds from Dunaliella salina microalga is optimized by combining pressurized liquid extraction (PLE) and experimental design (three-level factorial design) with three different solvents (hexane, ethanol, and water). Two main factors were considered, the extraction temperature (40, 100, and 160 °C) and the extraction time (5, 17.5, and 30 min). As response variables, the extraction yield (percent dry weight/initial weight) and the antioxidant activity of the extracts (determined using the TEAC method) were used. The parameters of the model were estimated by multiple linear regression. Results showed that the extraction temperature was the factor having the strongest influence (positive) on the two response variables. The best yields were obtained with ethanol at the higher extraction temperature and time tested. Besides, although hexane extracts provided the best antioxidant activity, ethanol extracts were also very active. The chemical characterization of ethanol extracts was carried out using HPLC-DAD, and attempts have been made to correlate their chemical composition with the antioxidant activity measured. Results pointed out that the extracts contained, besides all-trans-β-carotene and isomers, several different minor carotenoids that seemed to make a contribution to the antioxidant activity of the extracts. Keywords: PLE; Dunaliella salina; TEAC; antioxidant compounds; optimization</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16848551</pmid><doi>10.1021/jf060546q</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | antioxidant activity antioxidants Antioxidants - isolation & purification Biological and medical sciences carotenoids Chlorophyta - chemistry Chromatography, High Pressure Liquid Dunaliella salina ethanol extraction Food engineering Food industries Fundamental and applied biological sciences. Psychology General aspects hexane Linear Models Pressure pressure treatment pressurized liquid extraction Solutions Solvents Temperature Time Factors water |
| title | Optimization of the Extraction of Antioxidants from Dunaliella salina Microalga by Pressurized Liquids |
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