Identification of peptides specific for antibodies in vitiligo using a phage library
Summary Patients with vitiligo produce specific autoantibodies that can be detected in their sera. These antibodies are believed to play a role in the pathogenesis of this disease. A random peptide library displayed on phage is a technique that can be used to identify the epitopes that react with mo...
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| Veröffentlicht in: | Clinical and experimental dermatology 2005-11, Vol.30 (6), p.694-701 |
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| container_title | Clinical and experimental dermatology |
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| creator | Jadali, Z. Eslami, M. B. Sanati, M. H. Mansouri, P. Mahmoudi, M. Maghsoudi, . N. Esfahanian, F. |
| description | Summary
Patients with vitiligo produce specific autoantibodies that can be detected in their sera. These antibodies are believed to play a role in the pathogenesis of this disease. A random peptide library displayed on phage is a technique that can be used to identify the epitopes that react with monoclonal and polyclonal antibodies. We used this technique to identify the epitopes that react specifically with the vitiligo autoantibodies. By screening the random peptide phage library and using ELISA, two clones that showed a higher frequency of reactivity with the antibodies in the sera of patients with vitiligo were identified. The peptides do not show any similarity with the autoantigens so far implicated in vitiligo, indicating that these epitopes may mimic conformational epitopes in proteins. |
| doi_str_mv | 10.1111/j.1365-2230.2005.01903.x |
| format | Article |
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Patients with vitiligo produce specific autoantibodies that can be detected in their sera. These antibodies are believed to play a role in the pathogenesis of this disease. A random peptide library displayed on phage is a technique that can be used to identify the epitopes that react with monoclonal and polyclonal antibodies. We used this technique to identify the epitopes that react specifically with the vitiligo autoantibodies. By screening the random peptide phage library and using ELISA, two clones that showed a higher frequency of reactivity with the antibodies in the sera of patients with vitiligo were identified. The peptides do not show any similarity with the autoantigens so far implicated in vitiligo, indicating that these epitopes may mimic conformational epitopes in proteins.</description><identifier>ISSN: 0307-6938</identifier><identifier>EISSN: 1365-2230</identifier><identifier>DOI: 10.1111/j.1365-2230.2005.01903.x</identifier><identifier>PMID: 16197391</identifier><identifier>CODEN: CEDEDE</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Adolescent ; Adult ; Amino Acid Sequence ; Autoantibodies - blood ; Bacteriophage M13 - immunology ; Biological and medical sciences ; Dermatology ; Enzyme-Linked Immunosorbent Assay ; Epitope Mapping - methods ; Epitopes ; Female ; Humans ; Immunoglobulins - immunology ; Male ; Medical sciences ; Middle Aged ; Peptide Library ; Pigmentary diseases of the skin ; Vitiligo - immunology</subject><ispartof>Clinical and experimental dermatology, 2005-11, Vol.30 (6), p.694-701</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Nov 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4623-59f0e70f34283da65d8f6a21477f956d7b3278938af5fc180eea7f2f73d7831d3</citedby><cites>FETCH-LOGICAL-c4623-59f0e70f34283da65d8f6a21477f956d7b3278938af5fc180eea7f2f73d7831d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17179477$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16197391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jadali, Z.</creatorcontrib><creatorcontrib>Eslami, M. B.</creatorcontrib><creatorcontrib>Sanati, M. H.</creatorcontrib><creatorcontrib>Mansouri, P.</creatorcontrib><creatorcontrib>Mahmoudi, M.</creatorcontrib><creatorcontrib>Maghsoudi, . N.</creatorcontrib><creatorcontrib>Esfahanian, F.</creatorcontrib><title>Identification of peptides specific for antibodies in vitiligo using a phage library</title><title>Clinical and experimental dermatology</title><addtitle>Clin Exp Dermatol</addtitle><description>Summary
Patients with vitiligo produce specific autoantibodies that can be detected in their sera. These antibodies are believed to play a role in the pathogenesis of this disease. A random peptide library displayed on phage is a technique that can be used to identify the epitopes that react with monoclonal and polyclonal antibodies. We used this technique to identify the epitopes that react specifically with the vitiligo autoantibodies. By screening the random peptide phage library and using ELISA, two clones that showed a higher frequency of reactivity with the antibodies in the sera of patients with vitiligo were identified. The peptides do not show any similarity with the autoantigens so far implicated in vitiligo, indicating that these epitopes may mimic conformational epitopes in proteins.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Amino Acid Sequence</subject><subject>Autoantibodies - blood</subject><subject>Bacteriophage M13 - immunology</subject><subject>Biological and medical sciences</subject><subject>Dermatology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitope Mapping - methods</subject><subject>Epitopes</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulins - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Peptide Library</subject><subject>Pigmentary diseases of the skin</subject><subject>Vitiligo - immunology</subject><issn>0307-6938</issn><issn>1365-2230</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1v1DAQhi0EotvCX0AWEtwSbM_6IwcOaClt1Qo4FHG0vLG9eMnGwU5g--_rsKtW4oQvtjzPO5p5EMKU1LScd9uaguAVY0BqRgivCW0I1PsnaPFQeIoWBIisRAPqBJ3mvCWEApX8OTqhgjYSGrpAt1fW9WPwoTVjiD2OHg9uGIN1GefBtXMF-5iwKdQ62lD-Q49_hzF0YRPxlEO_wQYPP8zG4S6sk0l3L9Azb7rsXh7vM_Tt0_nt6rK6-XJxtfpwU7VLwaDijSdOEg9LpsAawa3ywjC6lNI3XFi5BiZVGd947luqiHNGeuYlWKmAWjhDbw99hxR_TS6Pehdy67rO9C5OWQsllkooVcDX_4DbOKW-zKYZoYw3is2QOkBtijkn5_WQwq6soynRs3a91bNdPdvVs3b9V7vel-irY_9pvXP2MXj0XIA3R8Dk1nQ-mb4N-ZGTVDZl7cK9P3B_Qufu_nsAvTr_OL9KvjrkQx7d_iFv0k8tJEiuv3--0NfX4lIB_6oZ3AMVqKuV</recordid><startdate>200511</startdate><enddate>200511</enddate><creator>Jadali, Z.</creator><creator>Eslami, M. B.</creator><creator>Sanati, M. H.</creator><creator>Mansouri, P.</creator><creator>Mahmoudi, M.</creator><creator>Maghsoudi, . N.</creator><creator>Esfahanian, F.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>200511</creationdate><title>Identification of peptides specific for antibodies in vitiligo using a phage library</title><author>Jadali, Z. ; Eslami, M. B. ; Sanati, M. H. ; Mansouri, P. ; Mahmoudi, M. ; Maghsoudi, . N. ; Esfahanian, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4623-59f0e70f34283da65d8f6a21477f956d7b3278938af5fc180eea7f2f73d7831d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Amino Acid Sequence</topic><topic>Autoantibodies - blood</topic><topic>Bacteriophage M13 - immunology</topic><topic>Biological and medical sciences</topic><topic>Dermatology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitope Mapping - methods</topic><topic>Epitopes</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulins - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Peptide Library</topic><topic>Pigmentary diseases of the skin</topic><topic>Vitiligo - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jadali, Z.</creatorcontrib><creatorcontrib>Eslami, M. B.</creatorcontrib><creatorcontrib>Sanati, M. H.</creatorcontrib><creatorcontrib>Mansouri, P.</creatorcontrib><creatorcontrib>Mahmoudi, M.</creatorcontrib><creatorcontrib>Maghsoudi, . N.</creatorcontrib><creatorcontrib>Esfahanian, F.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jadali, Z.</au><au>Eslami, M. B.</au><au>Sanati, M. H.</au><au>Mansouri, P.</au><au>Mahmoudi, M.</au><au>Maghsoudi, . N.</au><au>Esfahanian, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of peptides specific for antibodies in vitiligo using a phage library</atitle><jtitle>Clinical and experimental dermatology</jtitle><addtitle>Clin Exp Dermatol</addtitle><date>2005-11</date><risdate>2005</risdate><volume>30</volume><issue>6</issue><spage>694</spage><epage>701</epage><pages>694-701</pages><issn>0307-6938</issn><eissn>1365-2230</eissn><coden>CEDEDE</coden><abstract>Summary
Patients with vitiligo produce specific autoantibodies that can be detected in their sera. These antibodies are believed to play a role in the pathogenesis of this disease. A random peptide library displayed on phage is a technique that can be used to identify the epitopes that react with monoclonal and polyclonal antibodies. We used this technique to identify the epitopes that react specifically with the vitiligo autoantibodies. By screening the random peptide phage library and using ELISA, two clones that showed a higher frequency of reactivity with the antibodies in the sera of patients with vitiligo were identified. The peptides do not show any similarity with the autoantigens so far implicated in vitiligo, indicating that these epitopes may mimic conformational epitopes in proteins.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>16197391</pmid><doi>10.1111/j.1365-2230.2005.01903.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Clinical and experimental dermatology, 2005-11, Vol.30 (6), p.694-701 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adolescent Adult Amino Acid Sequence Autoantibodies - blood Bacteriophage M13 - immunology Biological and medical sciences Dermatology Enzyme-Linked Immunosorbent Assay Epitope Mapping - methods Epitopes Female Humans Immunoglobulins - immunology Male Medical sciences Middle Aged Peptide Library Pigmentary diseases of the skin Vitiligo - immunology |
| title | Identification of peptides specific for antibodies in vitiligo using a phage library |
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