Factors affecting volume reduction and red blood cell depletion of bone marrow on the COBE Spectra cell separator before haematopoietic stem cell transplantation

The COBE Spectra is used to volume/red blood cell (RBC) deplete BM before transplantation or cryopreservation. We have audited our results to identify the effect of transit time, refrigerated storage, age and cellular composition on mononuclear cells (MNC) and CD34+ cell recoveries, volume/RBC deple...

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Veröffentlicht in:	Bone marrow transplantation (Basingstoke) 2006-08, Vol.38 (3), p.175-181
Hauptverfasser:	GUTTRIDGE, M. G, SIDDERS, C, BOOTH-DAVEY, E, PAMPHILON, D, WATT, S. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Age Ambient temperature Analysis Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Antigens, CD34 Autografts Biological and medical sciences Blood Blood Preservation Bone marrow Bone Marrow Purging - instrumentation Bone Marrow Transplantation Bone marrow, stem cells transplantation. Graft versus host reaction CD34 antigen Cell separation Cell Separation - instrumentation Cold storage Collection Cryopreservation Depletion Engraftment Erythrocyte Volume Erythrocytes Hematopoietic Stem Cell Transplantation Hematopoietic stem cells Hematopoietic Stem Cells - cytology Humans Leukocyte Count - instrumentation Leukocytes (mononuclear) Leukocytes (neutrophilic) Leukocytes, Mononuclear - cytology Medical examination Medical sciences Methods Neutrophils Neutrophils - transplantation Protection and preservation Recovery Refrigeration Separators Stem cell transplantation Stem cells Time Factors Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transit time Transplantation Transplantation, Autologous Transplantation, Homologous
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