Factors affecting volume reduction and red blood cell depletion of bone marrow on the COBE Spectra cell separator before haematopoietic stem cell transplantation
The COBE Spectra is used to volume/red blood cell (RBC) deplete BM before transplantation or cryopreservation. We have audited our results to identify the effect of transit time, refrigerated storage, age and cellular composition on mononuclear cells (MNC) and CD34+ cell recoveries, volume/RBC deple...
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| Veröffentlicht in: | Bone marrow transplantation (Basingstoke) 2006-08, Vol.38 (3), p.175-181 |
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| creator | GUTTRIDGE, M. G SIDDERS, C BOOTH-DAVEY, E PAMPHILON, D WATT, S. M |
| description | The COBE Spectra is used to volume/red blood cell (RBC) deplete BM before transplantation or cryopreservation. We have audited our results to identify the effect of transit time, refrigerated storage, age and cellular composition on mononuclear cells (MNC) and CD34+ cell recoveries, volume/RBC depletion and neutrophil engraftment. In total, 88 consecutive collections from autologous (n = 25) and allogeneic (n = 63) donors were included. The mean collection volume was 1250 +/- 398 ml with RBC content of 341 +/- 113 ml. The MNC and CD34+ cell recoveries were 83.3 +/- 18.5 and 88.1 +/- 18.9%, respectively, volume depletion was 88.2+/-4.4% and RBC depletion 98.3 +/- 1.8%. Neutrophil engraftment was achieved in 20.1 +/- 6.4 days. Factors affecting MNC and CD34+ cell recoveries were transit time (P = 0.0060), overall age (P < 0.0210) and MNC/CD34+ cell concentrations (P < 0.0313). The presence of crenated RBC also reduced CD34+ cell recovery (P = 0.0028). Refrigerated storage did not adversely affect cell recovery (P > 0.8161) or neutrophil engraftment (P = 0.8959). This study demonstrates that time in transit, overall age, MNC and CD34+ cell concentrations and RBC condition were important factors affecting processing. RBCs show artefacts soon after collection at ambient temperatures and these may interfere with the separation and collection of MNC/CD34+ cells. Refrigeration at 4-6 degrees C during transit and storage may reduce formation of RBC artefacts and maximize MNC and CD34+ cell recoveries. |
| doi_str_mv | 10.1038/sj.bmt.1705420 |
| format | Article |
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G ; SIDDERS, C ; BOOTH-DAVEY, E ; PAMPHILON, D ; WATT, S. M</creator><creatorcontrib>GUTTRIDGE, M. G ; SIDDERS, C ; BOOTH-DAVEY, E ; PAMPHILON, D ; WATT, S. M</creatorcontrib><description>The COBE Spectra is used to volume/red blood cell (RBC) deplete BM before transplantation or cryopreservation. We have audited our results to identify the effect of transit time, refrigerated storage, age and cellular composition on mononuclear cells (MNC) and CD34+ cell recoveries, volume/RBC depletion and neutrophil engraftment. In total, 88 consecutive collections from autologous (n = 25) and allogeneic (n = 63) donors were included. The mean collection volume was 1250 +/- 398 ml with RBC content of 341 +/- 113 ml. The MNC and CD34+ cell recoveries were 83.3 +/- 18.5 and 88.1 +/- 18.9%, respectively, volume depletion was 88.2+/-4.4% and RBC depletion 98.3 +/- 1.8%. Neutrophil engraftment was achieved in 20.1 +/- 6.4 days. Factors affecting MNC and CD34+ cell recoveries were transit time (P = 0.0060), overall age (P < 0.0210) and MNC/CD34+ cell concentrations (P < 0.0313). The presence of crenated RBC also reduced CD34+ cell recovery (P = 0.0028). Refrigerated storage did not adversely affect cell recovery (P > 0.8161) or neutrophil engraftment (P = 0.8959). This study demonstrates that time in transit, overall age, MNC and CD34+ cell concentrations and RBC condition were important factors affecting processing. RBCs show artefacts soon after collection at ambient temperatures and these may interfere with the separation and collection of MNC/CD34+ cells. Refrigeration at 4-6 degrees C during transit and storage may reduce formation of RBC artefacts and maximize MNC and CD34+ cell recoveries.</description><identifier>ISSN: 0268-3369</identifier><identifier>EISSN: 1476-5365</identifier><identifier>DOI: 10.1038/sj.bmt.1705420</identifier><identifier>PMID: 16770313</identifier><identifier>CODEN: BMTRE9</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing Group</publisher><subject>Age ; Ambient temperature ; Analysis ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Antigens, CD34 ; Autografts ; Biological and medical sciences ; Blood ; Blood Preservation ; Bone marrow ; Bone Marrow Purging - instrumentation ; Bone Marrow Transplantation ; Bone marrow, stem cells transplantation. Graft versus host reaction ; CD34 antigen ; Cell separation ; Cell Separation - instrumentation ; Cold storage ; Collection ; Cryopreservation ; Depletion ; Engraftment ; Erythrocyte Volume ; Erythrocytes ; Hematopoietic Stem Cell Transplantation ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Humans ; Leukocyte Count - instrumentation ; Leukocytes (mononuclear) ; Leukocytes (neutrophilic) ; Leukocytes, Mononuclear - cytology ; Medical examination ; Medical sciences ; Methods ; Neutrophils ; Neutrophils - transplantation ; Protection and preservation ; Recovery ; Refrigeration ; Separators ; Stem cell transplantation ; Stem cells ; Time Factors ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Transit time ; Transplantation ; Transplantation, Autologous ; Transplantation, Homologous</subject><ispartof>Bone marrow transplantation (Basingstoke), 2006-08, Vol.38 (3), p.175-181</ispartof><rights>2006 INIST-CNRS</rights><rights>COPYRIGHT 2006 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Aug 2006</rights><rights>Nature Publishing Group 2006.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-8baf25951c01fde8e7e4230413ab1ae2557b0d6613a8f2b04f2ffe84349fac003</citedby><cites>FETCH-LOGICAL-c529t-8baf25951c01fde8e7e4230413ab1ae2557b0d6613a8f2b04f2ffe84349fac003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27913,27914</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17988931$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16770313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GUTTRIDGE, M. G</creatorcontrib><creatorcontrib>SIDDERS, C</creatorcontrib><creatorcontrib>BOOTH-DAVEY, E</creatorcontrib><creatorcontrib>PAMPHILON, D</creatorcontrib><creatorcontrib>WATT, S. M</creatorcontrib><title>Factors affecting volume reduction and red blood cell depletion of bone marrow on the COBE Spectra cell separator before haematopoietic stem cell transplantation</title><title>Bone marrow transplantation (Basingstoke)</title><addtitle>Bone Marrow Transplant</addtitle><description>The COBE Spectra is used to volume/red blood cell (RBC) deplete BM before transplantation or cryopreservation. We have audited our results to identify the effect of transit time, refrigerated storage, age and cellular composition on mononuclear cells (MNC) and CD34+ cell recoveries, volume/RBC depletion and neutrophil engraftment. In total, 88 consecutive collections from autologous (n = 25) and allogeneic (n = 63) donors were included. The mean collection volume was 1250 +/- 398 ml with RBC content of 341 +/- 113 ml. The MNC and CD34+ cell recoveries were 83.3 +/- 18.5 and 88.1 +/- 18.9%, respectively, volume depletion was 88.2+/-4.4% and RBC depletion 98.3 +/- 1.8%. Neutrophil engraftment was achieved in 20.1 +/- 6.4 days. Factors affecting MNC and CD34+ cell recoveries were transit time (P = 0.0060), overall age (P < 0.0210) and MNC/CD34+ cell concentrations (P < 0.0313). The presence of crenated RBC also reduced CD34+ cell recovery (P = 0.0028). Refrigerated storage did not adversely affect cell recovery (P > 0.8161) or neutrophil engraftment (P = 0.8959). This study demonstrates that time in transit, overall age, MNC and CD34+ cell concentrations and RBC condition were important factors affecting processing. RBCs show artefacts soon after collection at ambient temperatures and these may interfere with the separation and collection of MNC/CD34+ cells. Refrigeration at 4-6 degrees C during transit and storage may reduce formation of RBC artefacts and maximize MNC and CD34+ cell recoveries.</description><subject>Age</subject><subject>Ambient temperature</subject><subject>Analysis</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Antigens, CD34</subject><subject>Autografts</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Blood Preservation</subject><subject>Bone marrow</subject><subject>Bone Marrow Purging - instrumentation</subject><subject>Bone Marrow Transplantation</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>CD34 antigen</subject><subject>Cell separation</subject><subject>Cell Separation - instrumentation</subject><subject>Cold storage</subject><subject>Collection</subject><subject>Cryopreservation</subject><subject>Depletion</subject><subject>Engraftment</subject><subject>Erythrocyte Volume</subject><subject>Erythrocytes</subject><subject>Hematopoietic Stem Cell Transplantation</subject><subject>Hematopoietic stem cells</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Humans</subject><subject>Leukocyte Count - instrumentation</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes (neutrophilic)</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Medical examination</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Neutrophils</subject><subject>Neutrophils - transplantation</subject><subject>Protection and preservation</subject><subject>Recovery</subject><subject>Refrigeration</subject><subject>Separators</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Time Factors</subject><subject>Transfusions. Complications. Transfusion reactions. 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G</au><au>SIDDERS, C</au><au>BOOTH-DAVEY, E</au><au>PAMPHILON, D</au><au>WATT, S. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Factors affecting volume reduction and red blood cell depletion of bone marrow on the COBE Spectra cell separator before haematopoietic stem cell transplantation</atitle><jtitle>Bone marrow transplantation (Basingstoke)</jtitle><addtitle>Bone Marrow Transplant</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>38</volume><issue>3</issue><spage>175</spage><epage>181</epage><pages>175-181</pages><issn>0268-3369</issn><eissn>1476-5365</eissn><coden>BMTRE9</coden><abstract>The COBE Spectra is used to volume/red blood cell (RBC) deplete BM before transplantation or cryopreservation. We have audited our results to identify the effect of transit time, refrigerated storage, age and cellular composition on mononuclear cells (MNC) and CD34+ cell recoveries, volume/RBC depletion and neutrophil engraftment. In total, 88 consecutive collections from autologous (n = 25) and allogeneic (n = 63) donors were included. The mean collection volume was 1250 +/- 398 ml with RBC content of 341 +/- 113 ml. The MNC and CD34+ cell recoveries were 83.3 +/- 18.5 and 88.1 +/- 18.9%, respectively, volume depletion was 88.2+/-4.4% and RBC depletion 98.3 +/- 1.8%. Neutrophil engraftment was achieved in 20.1 +/- 6.4 days. Factors affecting MNC and CD34+ cell recoveries were transit time (P = 0.0060), overall age (P < 0.0210) and MNC/CD34+ cell concentrations (P < 0.0313). The presence of crenated RBC also reduced CD34+ cell recovery (P = 0.0028). Refrigerated storage did not adversely affect cell recovery (P > 0.8161) or neutrophil engraftment (P = 0.8959). This study demonstrates that time in transit, overall age, MNC and CD34+ cell concentrations and RBC condition were important factors affecting processing. RBCs show artefacts soon after collection at ambient temperatures and these may interfere with the separation and collection of MNC/CD34+ cells. Refrigeration at 4-6 degrees C during transit and storage may reduce formation of RBC artefacts and maximize MNC and CD34+ cell recoveries.</abstract><cop>Basingstoke</cop><pub>Nature Publishing Group</pub><pmid>16770313</pmid><doi>10.1038/sj.bmt.1705420</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Bone marrow transplantation (Basingstoke), 2006-08, Vol.38 (3), p.175-181 |
| issn | 0268-3369 1476-5365 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | Age Ambient temperature Analysis Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Antigens, CD34 Autografts Biological and medical sciences Blood Blood Preservation Bone marrow Bone Marrow Purging - instrumentation Bone Marrow Transplantation Bone marrow, stem cells transplantation. Graft versus host reaction CD34 antigen Cell separation Cell Separation - instrumentation Cold storage Collection Cryopreservation Depletion Engraftment Erythrocyte Volume Erythrocytes Hematopoietic Stem Cell Transplantation Hematopoietic stem cells Hematopoietic Stem Cells - cytology Humans Leukocyte Count - instrumentation Leukocytes (mononuclear) Leukocytes (neutrophilic) Leukocytes, Mononuclear - cytology Medical examination Medical sciences Methods Neutrophils Neutrophils - transplantation Protection and preservation Recovery Refrigeration Separators Stem cell transplantation Stem cells Time Factors Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transit time Transplantation Transplantation, Autologous Transplantation, Homologous |
| title | Factors affecting volume reduction and red blood cell depletion of bone marrow on the COBE Spectra cell separator before haematopoietic stem cell transplantation |
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