Biomolecular sensor based on fluorescence-labeled aptamer
Fluorescence-labeled aptamer for l-argininamide was developed by a combination of DNA aptamer and fluorophore–quencher pairs. The fluorescence intensity of the aptamer was reduced to about one-fifth by the addition of l-argininamide. The binding affinities of the modified aptamers were nearly equal...
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| Veröffentlicht in: | Bioorganic & medicinal chemistry letters 2006-08, Vol.16 (16), p.4381-4384 |
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| container_title | Bioorganic & medicinal chemistry letters |
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| creator | Ozaki, Hiroaki Nishihira, Akifumi Wakabayashi, Masayuki Kuwahara, Masayasu Sawai, Hiroaki |
| description | Fluorescence-labeled aptamer for
l-argininamide was developed by a combination of DNA aptamer and fluorophore–quencher pairs. The fluorescence intensity of the aptamer was reduced to about one-fifth by the addition of
l-argininamide. The binding affinities of the modified aptamers were nearly equal to that of its original aptamer.
Fluorescent DNA probes for
l-argininamide were developed by a combination of DNA aptamers and fluorophore–quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect
l-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities. |
| doi_str_mv | 10.1016/j.bmcl.2006.05.054 |
| format | Article |
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l-argininamide was developed by a combination of DNA aptamer and fluorophore–quencher pairs. The fluorescence intensity of the aptamer was reduced to about one-fifth by the addition of
l-argininamide. The binding affinities of the modified aptamers were nearly equal to that of its original aptamer.
Fluorescent DNA probes for
l-argininamide were developed by a combination of DNA aptamers and fluorophore–quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect
l-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities.</description><identifier>ISSN: 0960-894X</identifier><identifier>EISSN: 1464-3405</identifier><identifier>DOI: 10.1016/j.bmcl.2006.05.054</identifier><identifier>PMID: 16757168</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Analytical, structural and metabolic biochemistry ; Aptamers, Nucleotide - chemistry ; Aptamers, Peptide - chemistry ; Argininamide ; Arginine - analogs & derivatives ; Arginine - chemistry ; Base Sequence ; Biological and medical sciences ; Biosensing Techniques ; Chemistry, Pharmaceutical - methods ; DNA - chemistry ; DNA aptamer ; Drug Design ; Fluorescence-labeled DNA ; Fluorescent Dyes - pharmacology ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; Ligands ; Models, Chemical ; Molecular Sequence Data ; Proteins ; Spectrometry, Fluorescence - methods ; Temperature</subject><ispartof>Bioorganic & medicinal chemistry letters, 2006-08, Vol.16 (16), p.4381-4384</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-78991160ccfff24ee009efaa1adf40e7a828a0b82893cb9e6e6e45426569cbea3</citedby><cites>FETCH-LOGICAL-c384t-78991160ccfff24ee009efaa1adf40e7a828a0b82893cb9e6e6e45426569cbea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bmcl.2006.05.054$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17953173$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16757168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ozaki, Hiroaki</creatorcontrib><creatorcontrib>Nishihira, Akifumi</creatorcontrib><creatorcontrib>Wakabayashi, Masayuki</creatorcontrib><creatorcontrib>Kuwahara, Masayasu</creatorcontrib><creatorcontrib>Sawai, Hiroaki</creatorcontrib><title>Biomolecular sensor based on fluorescence-labeled aptamer</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>Fluorescence-labeled aptamer for
l-argininamide was developed by a combination of DNA aptamer and fluorophore–quencher pairs. The fluorescence intensity of the aptamer was reduced to about one-fifth by the addition of
l-argininamide. The binding affinities of the modified aptamers were nearly equal to that of its original aptamer.
Fluorescent DNA probes for
l-argininamide were developed by a combination of DNA aptamers and fluorophore–quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect
l-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Aptamers, Peptide - chemistry</subject><subject>Argininamide</subject><subject>Arginine - analogs & derivatives</subject><subject>Arginine - chemistry</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Chemistry, Pharmaceutical - methods</subject><subject>DNA - chemistry</subject><subject>DNA aptamer</subject><subject>Drug Design</subject><subject>Fluorescence-labeled DNA</subject><subject>Fluorescent Dyes - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Ligands</subject><subject>Models, Chemical</subject><subject>Molecular Sequence Data</subject><subject>Proteins</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Temperature</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQQIMotlb_gAfpRW9bJ7vZ7Aa8aPELCl4UvIXZ7AS2ZDc16Qr-e1Na8CYzzMDwZhgeY5ccFhy4vF0vmt64RQ4gF1CmFEdsyoUUWSGgPGZTUBKyWonPCTuLcQ3ABQhxyiZcVmXFZT1l6qHzvXdkRodhHmmIPswbjNTO_TC3bvSBoqHBUOawIZfmuNliT-GcnVh0kS4OfcY-nh7fly_Z6u35dXm_ykxRi21W1UpxLsEYa20uiAAUWUSOrRVAFdZ5jdCkqgrTKJIpRClyWUplGsJixm72dzfBf40Ut7rv0kfO4UB-jFrWUuRK1QnM96AJPsZAVm9C12P40Rz0Tphe650wvROmoUwp0tLV4frY9NT-rRwMJeD6AGA06GzAwXTxj6tUWfCqSNzdnqPk4rujoKPpdt7aLpDZ6tZ3__3xC8FriYY</recordid><startdate>20060815</startdate><enddate>20060815</enddate><creator>Ozaki, Hiroaki</creator><creator>Nishihira, Akifumi</creator><creator>Wakabayashi, Masayuki</creator><creator>Kuwahara, Masayasu</creator><creator>Sawai, Hiroaki</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060815</creationdate><title>Biomolecular sensor based on fluorescence-labeled aptamer</title><author>Ozaki, Hiroaki ; Nishihira, Akifumi ; Wakabayashi, Masayuki ; Kuwahara, Masayasu ; Sawai, Hiroaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-78991160ccfff24ee009efaa1adf40e7a828a0b82893cb9e6e6e45426569cbea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Aptamers, Peptide - chemistry</topic><topic>Argininamide</topic><topic>Arginine - analogs & derivatives</topic><topic>Arginine - chemistry</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Chemistry, Pharmaceutical - methods</topic><topic>DNA - chemistry</topic><topic>DNA aptamer</topic><topic>Drug Design</topic><topic>Fluorescence-labeled DNA</topic><topic>Fluorescent Dyes - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Ligands</topic><topic>Models, Chemical</topic><topic>Molecular Sequence Data</topic><topic>Proteins</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ozaki, Hiroaki</creatorcontrib><creatorcontrib>Nishihira, Akifumi</creatorcontrib><creatorcontrib>Wakabayashi, Masayuki</creatorcontrib><creatorcontrib>Kuwahara, Masayasu</creatorcontrib><creatorcontrib>Sawai, Hiroaki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic & medicinal chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ozaki, Hiroaki</au><au>Nishihira, Akifumi</au><au>Wakabayashi, Masayuki</au><au>Kuwahara, Masayasu</au><au>Sawai, Hiroaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biomolecular sensor based on fluorescence-labeled aptamer</atitle><jtitle>Bioorganic & medicinal chemistry letters</jtitle><addtitle>Bioorg Med Chem Lett</addtitle><date>2006-08-15</date><risdate>2006</risdate><volume>16</volume><issue>16</issue><spage>4381</spage><epage>4384</epage><pages>4381-4384</pages><issn>0960-894X</issn><eissn>1464-3405</eissn><abstract>Fluorescence-labeled aptamer for
l-argininamide was developed by a combination of DNA aptamer and fluorophore–quencher pairs. The fluorescence intensity of the aptamer was reduced to about one-fifth by the addition of
l-argininamide. The binding affinities of the modified aptamers were nearly equal to that of its original aptamer.
Fluorescent DNA probes for
l-argininamide were developed by a combination of DNA aptamers and fluorophore–quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect
l-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16757168</pmid><doi>10.1016/j.bmcl.2006.05.054</doi><tpages>4</tpages></addata></record> |
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| language | eng |
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| subjects | Analytical, structural and metabolic biochemistry Aptamers, Nucleotide - chemistry Aptamers, Peptide - chemistry Argininamide Arginine - analogs & derivatives Arginine - chemistry Base Sequence Biological and medical sciences Biosensing Techniques Chemistry, Pharmaceutical - methods DNA - chemistry DNA aptamer Drug Design Fluorescence-labeled DNA Fluorescent Dyes - pharmacology Fundamental and applied biological sciences. Psychology General aspects, investigation methods Ligands Models, Chemical Molecular Sequence Data Proteins Spectrometry, Fluorescence - methods Temperature |
| title | Biomolecular sensor based on fluorescence-labeled aptamer |
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