Effect of γ-irradiation on the molecular properties of bovine serum albumin
To investigate the effect of oxygen radicals on the molecular properties of bovine serum albumin (BSA), the secondary and tertiary structures, molecular weight and optical anisotropy of BSA were examined after the irradiation of the protein at various doses. γ-Irradiation of the protein solution cau...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2005-08, Vol.100 (2), p.203-206 |
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| description | To investigate the effect of oxygen radicals on the molecular properties of bovine serum albumin (BSA), the secondary and tertiary structures, molecular weight and optical anisotropy of BSA were examined after the irradiation of the protein at various doses. γ-Irradiation of the protein solution caused the disruption of the ordered structure of protein molecules as well as degradation, cross-linking and aggregation of polypeptide chains. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. Fourier transform infrared spectroscopy (FTIR) indicated that irradiation caused transformation from β-turns into β-sheets. A light scattering study showed that increasing the radiation dose decreased the molecular weight of the protein. Optical anisotropy data showed that radiation changed the ordered structure of the protein. Ultraviolet absorption spectroscopy indicated that fragmentation and aggregation might occur in response to radiation exposure. |
| doi_str_mv | 10.1263/jbb.100.203 |
| format | Article |
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Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. Fourier transform infrared spectroscopy (FTIR) indicated that irradiation caused transformation from β-turns into β-sheets. A light scattering study showed that increasing the radiation dose decreased the molecular weight of the protein. Optical anisotropy data showed that radiation changed the ordered structure of the protein. Ultraviolet absorption spectroscopy indicated that fragmentation and aggregation might occur in response to radiation exposure.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1263/jbb.100.203</identifier><identifier>PMID: 16198265</identifier><language>eng</language><publisher>Amsterdarm: Elsevier B.V</publisher><subject>Absorption spectroscopy ; ALBUMINAS ; ALBUMINE ; ALBUMINS ; Anisotropy ; Biological and medical sciences ; Biotechnology ; BLOOD PROTEINS ; BOVINA ; BOVINAE ; Bovine serum albumin ; emission intensity ; fluorescence spectroscopy ; FREE RADICALS ; Fundamental and applied biological sciences. Psychology ; GAMMA IRRADIATION ; gamma Radiation ; Gamma Rays ; I.R. spectroscopy ; infrared absorption ; IRRADIACION GAMMA ; IRRADIATION GAMMA ; laser light scattering ; Light scattering ; molecular properties ; MOLECULAR WEIGHT ; OPTICAL PROPERTIES ; OXIGENO ; OXYGEN ; oxygen radicals ; OXYGENE ; PESO MOLECULAR ; POIDS MOLECULAIRE ; PROPIEDADES OPTICAS ; PROPRIETE OPTIQUE ; Protein structure ; Protein Structure, Secondary ; Protein Structure, Tertiary ; PROTEINAS SANGUINEAS ; PROTEINE SANGUINE ; Q1 ; Radiation ; RADICAL LIBRE ; RADICALES LIBRES ; Reactive oxygen species ; Serum Albumin, Bovine - chemistry ; Serum Albumin, Bovine - radiation effects ; Spectrometry, Fluorescence ; Spectrophotometry, Ultraviolet ; Spectroscopy, Fourier Transform Infrared ; U.V. radiation</subject><ispartof>Journal of bioscience and bioengineering, 2005-08, Vol.100 (2), p.203-206</ispartof><rights>2005 The Society for Biotechnology, Japan</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-a7768a05f6a0309ec3cd8e570bb5c60eb36806e54c8a2552fd351a7f73eb26c53</citedby><cites>FETCH-LOGICAL-c449t-a7768a05f6a0309ec3cd8e570bb5c60eb36806e54c8a2552fd351a7f73eb26c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1263/jbb.100.203$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17067552$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16198265$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gaber, Mohamed H.</creatorcontrib><title>Effect of γ-irradiation on the molecular properties of bovine serum albumin</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>To investigate the effect of oxygen radicals on the molecular properties of bovine serum albumin (BSA), the secondary and tertiary structures, molecular weight and optical anisotropy of BSA were examined after the irradiation of the protein at various doses. γ-Irradiation of the protein solution caused the disruption of the ordered structure of protein molecules as well as degradation, cross-linking and aggregation of polypeptide chains. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. Fourier transform infrared spectroscopy (FTIR) indicated that irradiation caused transformation from β-turns into β-sheets. A light scattering study showed that increasing the radiation dose decreased the molecular weight of the protein. Optical anisotropy data showed that radiation changed the ordered structure of the protein. Ultraviolet absorption spectroscopy indicated that fragmentation and aggregation might occur in response to radiation exposure.</description><subject>Absorption spectroscopy</subject><subject>ALBUMINAS</subject><subject>ALBUMINE</subject><subject>ALBUMINS</subject><subject>Anisotropy</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>BLOOD PROTEINS</subject><subject>BOVINA</subject><subject>BOVINAE</subject><subject>Bovine serum albumin</subject><subject>emission intensity</subject><subject>fluorescence spectroscopy</subject><subject>FREE RADICALS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAMMA IRRADIATION</subject><subject>gamma Radiation</subject><subject>Gamma Rays</subject><subject>I.R. spectroscopy</subject><subject>infrared absorption</subject><subject>IRRADIACION GAMMA</subject><subject>IRRADIATION GAMMA</subject><subject>laser light scattering</subject><subject>Light scattering</subject><subject>molecular properties</subject><subject>MOLECULAR WEIGHT</subject><subject>OPTICAL PROPERTIES</subject><subject>OXIGENO</subject><subject>OXYGEN</subject><subject>oxygen radicals</subject><subject>OXYGENE</subject><subject>PESO MOLECULAR</subject><subject>POIDS MOLECULAIRE</subject><subject>PROPIEDADES OPTICAS</subject><subject>PROPRIETE OPTIQUE</subject><subject>Protein structure</subject><subject>Protein Structure, Secondary</subject><subject>Protein Structure, Tertiary</subject><subject>PROTEINAS SANGUINEAS</subject><subject>PROTEINE SANGUINE</subject><subject>Q1</subject><subject>Radiation</subject><subject>RADICAL LIBRE</subject><subject>RADICALES LIBRES</subject><subject>Reactive oxygen species</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Serum Albumin, Bovine - radiation effects</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>U.V. radiation</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuKFDEUhoMozkzryrVSG0WQak-SyqWWwzDjhQZd6DokqRPNUFVpk6oBn8v38JlM0w2zEYVADuTj5-T_CHlGYUuZ5G9vndtSgC0D_oCcU96ptusYfXiYdd9SxfgZuSjlFoAqUPQxOaOS9ppJcU521yGgX5oUmt-_2pizHaJdYpqbepbv2ExpRL-ONjf7nPaYl4jlQLt0F2dsCuZ1auzo1inOT8ijYMeCT0_3hny9uf5y9b7dfXr34epy1_qu65fWKiW1BRGkBQ49eu4HjUKBc8JLQMelBomi89oyIVgYuKBWBcXRMekF35BXx9y60o8Vy2KmWDyOo50xrcVILTumajcb8vqfIFU91VpS3f03kyohhZZQwTdH0OdUSsZg9jlONv80FMxBiKlC6gymCqn0i1Ps6iYc7tmTgQq8PAG2eDuGbGcfyz2nQKraQeWeH7lgk7HfcmU-fmYAEmqH9PBVcXzHWvxdxGyKjzh7HGKugs2Q4l8X_AN7xK2B</recordid><startdate>20050801</startdate><enddate>20050801</enddate><creator>Gaber, Mohamed H.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050801</creationdate><title>Effect of γ-irradiation on the molecular properties of bovine serum albumin</title><author>Gaber, Mohamed H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-a7768a05f6a0309ec3cd8e570bb5c60eb36806e54c8a2552fd351a7f73eb26c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Absorption spectroscopy</topic><topic>ALBUMINAS</topic><topic>ALBUMINE</topic><topic>ALBUMINS</topic><topic>Anisotropy</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>BLOOD PROTEINS</topic><topic>BOVINA</topic><topic>BOVINAE</topic><topic>Bovine serum albumin</topic><topic>emission intensity</topic><topic>fluorescence spectroscopy</topic><topic>FREE RADICALS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAMMA IRRADIATION</topic><topic>gamma Radiation</topic><topic>Gamma Rays</topic><topic>I.R. spectroscopy</topic><topic>infrared absorption</topic><topic>IRRADIACION GAMMA</topic><topic>IRRADIATION GAMMA</topic><topic>laser light scattering</topic><topic>Light scattering</topic><topic>molecular properties</topic><topic>MOLECULAR WEIGHT</topic><topic>OPTICAL PROPERTIES</topic><topic>OXIGENO</topic><topic>OXYGEN</topic><topic>oxygen radicals</topic><topic>OXYGENE</topic><topic>PESO MOLECULAR</topic><topic>POIDS MOLECULAIRE</topic><topic>PROPIEDADES OPTICAS</topic><topic>PROPRIETE OPTIQUE</topic><topic>Protein structure</topic><topic>Protein Structure, Secondary</topic><topic>Protein Structure, Tertiary</topic><topic>PROTEINAS SANGUINEAS</topic><topic>PROTEINE SANGUINE</topic><topic>Q1</topic><topic>Radiation</topic><topic>RADICAL LIBRE</topic><topic>RADICALES LIBRES</topic><topic>Reactive oxygen species</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Serum Albumin, Bovine - radiation effects</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>U.V. radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaber, Mohamed H.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaber, Mohamed H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of γ-irradiation on the molecular properties of bovine serum albumin</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2005-08-01</date><risdate>2005</risdate><volume>100</volume><issue>2</issue><spage>203</spage><epage>206</epage><pages>203-206</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>To investigate the effect of oxygen radicals on the molecular properties of bovine serum albumin (BSA), the secondary and tertiary structures, molecular weight and optical anisotropy of BSA were examined after the irradiation of the protein at various doses. γ-Irradiation of the protein solution caused the disruption of the ordered structure of protein molecules as well as degradation, cross-linking and aggregation of polypeptide chains. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. Fourier transform infrared spectroscopy (FTIR) indicated that irradiation caused transformation from β-turns into β-sheets. A light scattering study showed that increasing the radiation dose decreased the molecular weight of the protein. Optical anisotropy data showed that radiation changed the ordered structure of the protein. Ultraviolet absorption spectroscopy indicated that fragmentation and aggregation might occur in response to radiation exposure.</abstract><cop>Amsterdarm</cop><pub>Elsevier B.V</pub><pmid>16198265</pmid><doi>10.1263/jbb.100.203</doi><tpages>4</tpages></addata></record> |
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| subjects | Absorption spectroscopy ALBUMINAS ALBUMINE ALBUMINS Anisotropy Biological and medical sciences Biotechnology BLOOD PROTEINS BOVINA BOVINAE Bovine serum albumin emission intensity fluorescence spectroscopy FREE RADICALS Fundamental and applied biological sciences. Psychology GAMMA IRRADIATION gamma Radiation Gamma Rays I.R. spectroscopy infrared absorption IRRADIACION GAMMA IRRADIATION GAMMA laser light scattering Light scattering molecular properties MOLECULAR WEIGHT OPTICAL PROPERTIES OXIGENO OXYGEN oxygen radicals OXYGENE PESO MOLECULAR POIDS MOLECULAIRE PROPIEDADES OPTICAS PROPRIETE OPTIQUE Protein structure Protein Structure, Secondary Protein Structure, Tertiary PROTEINAS SANGUINEAS PROTEINE SANGUINE Q1 Radiation RADICAL LIBRE RADICALES LIBRES Reactive oxygen species Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - radiation effects Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Spectroscopy, Fourier Transform Infrared U.V. radiation |
| title | Effect of γ-irradiation on the molecular properties of bovine serum albumin |
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