Orthogonality of Separation in Two-Dimensional Liquid Chromatography
Two-dimensional liquid chromatography is often used to reduce the proteomic sample complexity prior to tandem mass spectrometry analysis. The 2D-LC performance depends on the peak capacity in both chromatographic dimensions, and separation orthogonality. The peak capacity and selectivity of many LC...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2005-10, Vol.77 (19), p.6426-6434 |
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| creator | Gilar, Martin Olivova, Petra Daly, Amy E Gebler, John C |
| description | Two-dimensional liquid chromatography is often used to reduce the proteomic sample complexity prior to tandem mass spectrometry analysis. The 2D-LC performance depends on the peak capacity in both chromatographic dimensions, and separation orthogonality. The peak capacity and selectivity of many LC modes for peptides is not well known, and mathematical characterization for orthogonality is underdeveloped. Consequently, it is difficult to estimate the performance of 2D-LC for peptide separation. The goal of this paper was to investigate a selectivity of common LC modes and to identify the 2D-LC systems with a useful orthogonality. A geometric approach for orthogonality description was developed and applied for estimation of a practical peak 2D-LC capacity. Selected LC modes including various RP, SCX, SEC, and HILIC were combined in 2D-LC setups. SCX-RP, HILIC-RP, and RP-RP 2D systems were found to provide suitable orthogonality. The RP-RP system (employing significantly different pH in both RP separation dimensions) had the highest practical peak capacity of 2D-LC systems investigated. |
| doi_str_mv | 10.1021/ac050923i |
| format | Article |
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The 2D-LC performance depends on the peak capacity in both chromatographic dimensions, and separation orthogonality. The peak capacity and selectivity of many LC modes for peptides is not well known, and mathematical characterization for orthogonality is underdeveloped. Consequently, it is difficult to estimate the performance of 2D-LC for peptide separation. The goal of this paper was to investigate a selectivity of common LC modes and to identify the 2D-LC systems with a useful orthogonality. A geometric approach for orthogonality description was developed and applied for estimation of a practical peak 2D-LC capacity. Selected LC modes including various RP, SCX, SEC, and HILIC were combined in 2D-LC setups. SCX-RP, HILIC-RP, and RP-RP 2D systems were found to provide suitable orthogonality. The RP-RP system (employing significantly different pH in both RP separation dimensions) had the highest practical peak capacity of 2D-LC systems investigated.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac050923i</identifier><identifier>PMID: 16194109</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography ; Chromatography, Liquid - methods ; Exact sciences and technology ; Mass Spectrometry ; Other chromatographic methods ; Peptides ; Peptides - chemistry ; Proteins - chemistry ; Spectrometric and optical methods ; Time Factors</subject><ispartof>Analytical chemistry (Washington), 2005-10, Vol.77 (19), p.6426-6434</ispartof><rights>Copyright © 2005 American Chemical Society</rights><rights>2006 INIST-CNRS</rights><rights>Copyright American Chemical Society Oct 1, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a474t-a3def75defbbae8f004b16ea6b6d1bc353737ccb235d4f8321ce27d60f5e41ee3</citedby><cites>FETCH-LOGICAL-a474t-a3def75defbbae8f004b16ea6b6d1bc353737ccb235d4f8321ce27d60f5e41ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac050923i$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac050923i$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17175647$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16194109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gilar, Martin</creatorcontrib><creatorcontrib>Olivova, Petra</creatorcontrib><creatorcontrib>Daly, Amy E</creatorcontrib><creatorcontrib>Gebler, John C</creatorcontrib><title>Orthogonality of Separation in Two-Dimensional Liquid Chromatography</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Two-dimensional liquid chromatography is often used to reduce the proteomic sample complexity prior to tandem mass spectrometry analysis. The 2D-LC performance depends on the peak capacity in both chromatographic dimensions, and separation orthogonality. The peak capacity and selectivity of many LC modes for peptides is not well known, and mathematical characterization for orthogonality is underdeveloped. Consequently, it is difficult to estimate the performance of 2D-LC for peptide separation. The goal of this paper was to investigate a selectivity of common LC modes and to identify the 2D-LC systems with a useful orthogonality. A geometric approach for orthogonality description was developed and applied for estimation of a practical peak 2D-LC capacity. Selected LC modes including various RP, SCX, SEC, and HILIC were combined in 2D-LC setups. SCX-RP, HILIC-RP, and RP-RP 2D systems were found to provide suitable orthogonality. The RP-RP system (employing significantly different pH in both RP separation dimensions) had the highest practical peak capacity of 2D-LC systems investigated.</description><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography</subject><subject>Chromatography, Liquid - methods</subject><subject>Exact sciences and technology</subject><subject>Mass Spectrometry</subject><subject>Other chromatographic methods</subject><subject>Peptides</subject><subject>Peptides - chemistry</subject><subject>Proteins - chemistry</subject><subject>Spectrometric and optical methods</subject><subject>Time Factors</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0E2P0zAQBmALgdhSOPAHUIQE0h4CM3Zsp0fU5UtUWtAWrtbEcbZekrhrJ1r67zdVq63EXjwHP3o18zL2GuEDAsePZEHCggv_hM1QcshVWfKnbAYAIuca4Iy9SOkGABFQPWdnqHBRICxm7OIyDptwHXpq_bDLQpNduS1FGnzoM99n67uQX_jO9cnvTbbyt6Ovs-Umho6GcB1pu9m9ZM8aapN7dZxz9vvL5_XyW766_Pp9-WmVU6GLISdRu0bL6akqcmUDUFSoHKlK1VhZIYUW2tqKC1kXTSk4Wsd1raCRrkDnxJy9P-RuY7gdXRpM55N1bUu9C2MyqlQF36fM2dv_4E0Y47R_Mhx1WQqNckLnB2RjSCm6xmyj7yjuDILZ92oeep3sm2PgWHWuPsljkRN4dwSULLVNpN76dHIatVTFfrP84Hwa3L-Hf4p_jZqul2b988r84H-Wv9RaGn3KJZtORzxe8B5BQJqz</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Gilar, Martin</creator><creator>Olivova, Petra</creator><creator>Daly, Amy E</creator><creator>Gebler, John C</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20051001</creationdate><title>Orthogonality of Separation in Two-Dimensional Liquid Chromatography</title><author>Gilar, Martin ; Olivova, Petra ; Daly, Amy E ; Gebler, John C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a474t-a3def75defbbae8f004b16ea6b6d1bc353737ccb235d4f8321ce27d60f5e41ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analytical chemistry</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography</topic><topic>Chromatography, Liquid - methods</topic><topic>Exact sciences and technology</topic><topic>Mass Spectrometry</topic><topic>Other chromatographic methods</topic><topic>Peptides</topic><topic>Peptides - chemistry</topic><topic>Proteins - chemistry</topic><topic>Spectrometric and optical methods</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gilar, Martin</creatorcontrib><creatorcontrib>Olivova, Petra</creatorcontrib><creatorcontrib>Daly, Amy E</creatorcontrib><creatorcontrib>Gebler, John C</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gilar, Martin</au><au>Olivova, Petra</au><au>Daly, Amy E</au><au>Gebler, John C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Orthogonality of Separation in Two-Dimensional Liquid Chromatography</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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A geometric approach for orthogonality description was developed and applied for estimation of a practical peak 2D-LC capacity. Selected LC modes including various RP, SCX, SEC, and HILIC were combined in 2D-LC setups. SCX-RP, HILIC-RP, and RP-RP 2D systems were found to provide suitable orthogonality. The RP-RP system (employing significantly different pH in both RP separation dimensions) had the highest practical peak capacity of 2D-LC systems investigated.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16194109</pmid><doi>10.1021/ac050923i</doi><tpages>9</tpages></addata></record> |
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| subjects | Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Chromatography, Liquid - methods Exact sciences and technology Mass Spectrometry Other chromatographic methods Peptides Peptides - chemistry Proteins - chemistry Spectrometric and optical methods Time Factors |
| title | Orthogonality of Separation in Two-Dimensional Liquid Chromatography |
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