A fluorescence polarization-based interaction assay for hypoxia-inducible factor prolyl hydroxylases

Oxygen-dependent ubiquitination and degradation of hypoxia-inducible factor 1α (HIF-1α) plays a central role in regulating transcriptional responses to hypoxia. This process requires hydroxylation of specific prolines in HIF-1α by HIF prolyl hydroxylase domain (PHD)-containing enzymes, leading to it...

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Veröffentlicht in:	Biochemical and biophysical research communications 2005-11, Vol.337 (1), p.275-280
Hauptverfasser:	Cho, Hyunju, Park, Hyunsung, Yang, Eun Gyeong
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding, Competitive Elongin Enzyme Inhibitors - pharmacology Fluorescence polarization Fluorescence Polarization - methods HIF-1α–VBC interaction Humans Hydroxylation Hydroxyproline Hypoxia-inducible factor Hypoxia-Inducible Factor 1, alpha Subunit Hypoxia-Inducible Factor-Proline Dioxygenases Immediate-Early Proteins - metabolism Peptides - chemistry Peptides - metabolism Procollagen-Proline Dioxygenase - metabolism Prolyl hydroxylase Transcription Factors - metabolism Tumor Suppressor Proteins - metabolism Ubiquitin-Protein Ligases - metabolism VBC Von Hippel-Lindau Tumor Suppressor Protein von Hippel–Lindau protein
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