Monolithic Column HPLC Separation of Intact Proteins Analyzed by LC-MALDI Using On-Plate Digestion: An Approach To Integrate Protein Separation and Identification

A method is developed to integrate a protein separation by monolithic capillary reversed-phase high-performance liquid chromatography to on-probe tryptic digestion for subsequent analyses by MALDI-TOF MS and MALDI-TOF/TOF MS. The method provides a means of directly interfacing separations to MALDI-M...

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Veröffentlicht in:	Analytical chemistry (Washington) 2006-07, Vol.78 (14), p.5198-5204
Hauptverfasser:	Zheng, Suping, Yoo, Chul, Delmotte, Nathanael, Miller, Fred R, Huber, Christian G, Lubman, David M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Exact sciences and technology Hydrophobic and Hydrophilic Interactions Molecular Sequence Data Molecular Weight Online Systems Other chromatographic methods Proteins Proteins - chemistry Proteins - isolation & purification Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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