Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair
Kawata, T., Ito, H., Saito, M., Uno, T., Okayasu, R., Liu, C., Kan'o, M., George, K. and Cucinotta, F. A. Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair. Radiat. Res. 164, 509–513 (2005). Caffeine sensitizes cells to ionizing radiation, and...
Gespeichert in:
| Veröffentlicht in: | Radiation research 2005-10, Vol.164 (4), p.509-513 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 513 |
|---|---|
| container_issue | 4 |
| container_start_page | 509 |
| container_title | Radiation research |
| container_volume | 164 |
| creator | Kawata, Tetsuya Ito, Hisao Saito, Masayoshi Uno, Takashi Okayasu, Ryuichi Liu, Cuihua Kan'o, Momoe George, Kerry Cucinotta, Francis A. |
| description | Kawata, T., Ito, H., Saito, M., Uno, T., Okayasu, R., Liu, C., Kan'o, M., George, K. and Cucinotta, F. A. Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair. Radiat. Res. 164, 509–513 (2005). Caffeine sensitizes cells to ionizing radiation, and this effect is believed to be associated with the disruption of DNA damage-responsive cell cycle checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining of DSBs is one of the underlying mechanisms of AT cell hyper-radiosensitivity. In this study, we investigated the effects of caffeine and radiation on nongrowing G0 normal human fibroblast cells by determining cell survival and scoring aberrations in calyculin A-induced G2 chromosomes. Results from the cell survival study indicate that after X-ray exposure G0 cells were sensitized by 24 h treatment with caffeine. Analysis of chromosome aberrations using FISH (fluorescence in situ hybridization) revealed a high frequency of aberrant cells and color junctions in the caffeine-treated cells. Since most DNA repair in nongrowing G0 cells is believed to result from nonhomologous end joining (NHEJ), caffeine may influence the fidelity of the NHEJ pathway in irradiated G0 cells. |
| doi_str_mv | 10.1667/RR3382.1 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_68632988</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>3581559</jstor_id><sourcerecordid>3581559</sourcerecordid><originalsourceid>FETCH-LOGICAL-b443t-22d687161f21a94dd749881c4c52e14048afdf0d302e0980dba59ffd6fb997173</originalsourceid><addsrcrecordid>eNp1kF9LwzAUxYMobk7BDyCSJ_GlM2n6J3mU4dxgKkwF30raJDOjS2bSCvXTm9Hhnny6XM6Pc889AFxiNMZZlt8tl4TQeIyPwBAzQqM0QckxGCJESJSnNB-AM-_XKOw4Y6dggDNM8yAMgZxwpaQ2Er5K43Wjf6SHz9YI_a2FNis4azfcwKkunS1r7hsPGws_4JJ3HpYdnBvRVjuOw5lefcKpk1-tNFUHrYJP2ju55dqdgxPFay8v9nME3qcPb5NZtHh5nE_uF1GZJKSJ4lhkNA_hVIw5S4TIE0YprpIqjSUOP1GuhEKCoFgiRpEoecqUEpkqGctxTkbgpvfdOhti-KbYaF_JuuZG2tYXGc1IHCwDeNuDlbM-hFTF1ukNd12BUbGrtOgrLXBAr_eebbmR4gDuOwzAVQ-sfWPdn05SitOUHTKV2loj_z_0C2rOhkM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68632988</pqid></control><display><type>article</type><title>Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair</title><source>MEDLINE</source><source>BioOne Complete</source><source>JSTOR Archive Collection A-Z Listing</source><creator>Kawata, Tetsuya ; Ito, Hisao ; Saito, Masayoshi ; Uno, Takashi ; Okayasu, Ryuichi ; Liu, Cuihua ; Kan'o, Momoe ; George, Kerry ; Cucinotta, Francis A.</creator><creatorcontrib>Kawata, Tetsuya ; Ito, Hisao ; Saito, Masayoshi ; Uno, Takashi ; Okayasu, Ryuichi ; Liu, Cuihua ; Kan'o, Momoe ; George, Kerry ; Cucinotta, Francis A.</creatorcontrib><description>Kawata, T., Ito, H., Saito, M., Uno, T., Okayasu, R., Liu, C., Kan'o, M., George, K. and Cucinotta, F. A. Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair. Radiat. Res. 164, 509–513 (2005). Caffeine sensitizes cells to ionizing radiation, and this effect is believed to be associated with the disruption of DNA damage-responsive cell cycle checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining of DSBs is one of the underlying mechanisms of AT cell hyper-radiosensitivity. In this study, we investigated the effects of caffeine and radiation on nongrowing G0 normal human fibroblast cells by determining cell survival and scoring aberrations in calyculin A-induced G2 chromosomes. Results from the cell survival study indicate that after X-ray exposure G0 cells were sensitized by 24 h treatment with caffeine. Analysis of chromosome aberrations using FISH (fluorescence in situ hybridization) revealed a high frequency of aberrant cells and color junctions in the caffeine-treated cells. Since most DNA repair in nongrowing G0 cells is believed to result from nonhomologous end joining (NHEJ), caffeine may influence the fidelity of the NHEJ pathway in irradiated G0 cells.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.1667/RR3382.1</identifier><identifier>PMID: 16187758</identifier><language>eng</language><publisher>United States: Radiation Research Society</publisher><subject>Ataxia telangiectasia ; Caffeine - pharmacology ; CELL BIOLOGY ; Cell cycle ; Cell Line ; Cell lines ; Chromosome Aberrations ; Chromosomes ; DNA damage ; DNA Repair - drug effects ; Fibroblasts ; Fibroblasts - drug effects ; Fibroblasts - radiation effects ; HeLa cells ; Humans ; Interphase ; Irradiation ; Lymphocytes ; Radiation-Sensitizing Agents - pharmacology ; Recombination, Genetic - drug effects ; Space life sciences ; X-Rays</subject><ispartof>Radiation research, 2005-10, Vol.164 (4), p.509-513</ispartof><rights>Radiation Research Society</rights><rights>Copyright 2005 The Radiation Research Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b443t-22d687161f21a94dd749881c4c52e14048afdf0d302e0980dba59ffd6fb997173</citedby><cites>FETCH-LOGICAL-b443t-22d687161f21a94dd749881c4c52e14048afdf0d302e0980dba59ffd6fb997173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1667/RR3382.1$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3581559$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,26978,27924,27925,52363,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16187758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawata, Tetsuya</creatorcontrib><creatorcontrib>Ito, Hisao</creatorcontrib><creatorcontrib>Saito, Masayoshi</creatorcontrib><creatorcontrib>Uno, Takashi</creatorcontrib><creatorcontrib>Okayasu, Ryuichi</creatorcontrib><creatorcontrib>Liu, Cuihua</creatorcontrib><creatorcontrib>Kan'o, Momoe</creatorcontrib><creatorcontrib>George, Kerry</creatorcontrib><creatorcontrib>Cucinotta, Francis A.</creatorcontrib><title>Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair</title><title>Radiation research</title><addtitle>Radiat Res</addtitle><description>Kawata, T., Ito, H., Saito, M., Uno, T., Okayasu, R., Liu, C., Kan'o, M., George, K. and Cucinotta, F. A. Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair. Radiat. Res. 164, 509–513 (2005). Caffeine sensitizes cells to ionizing radiation, and this effect is believed to be associated with the disruption of DNA damage-responsive cell cycle checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining of DSBs is one of the underlying mechanisms of AT cell hyper-radiosensitivity. In this study, we investigated the effects of caffeine and radiation on nongrowing G0 normal human fibroblast cells by determining cell survival and scoring aberrations in calyculin A-induced G2 chromosomes. Results from the cell survival study indicate that after X-ray exposure G0 cells were sensitized by 24 h treatment with caffeine. Analysis of chromosome aberrations using FISH (fluorescence in situ hybridization) revealed a high frequency of aberrant cells and color junctions in the caffeine-treated cells. Since most DNA repair in nongrowing G0 cells is believed to result from nonhomologous end joining (NHEJ), caffeine may influence the fidelity of the NHEJ pathway in irradiated G0 cells.</description><subject>Ataxia telangiectasia</subject><subject>Caffeine - pharmacology</subject><subject>CELL BIOLOGY</subject><subject>Cell cycle</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Chromosome Aberrations</subject><subject>Chromosomes</subject><subject>DNA damage</subject><subject>DNA Repair - drug effects</subject><subject>Fibroblasts</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - radiation effects</subject><subject>HeLa cells</subject><subject>Humans</subject><subject>Interphase</subject><subject>Irradiation</subject><subject>Lymphocytes</subject><subject>Radiation-Sensitizing Agents - pharmacology</subject><subject>Recombination, Genetic - drug effects</subject><subject>Space life sciences</subject><subject>X-Rays</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF9LwzAUxYMobk7BDyCSJ_GlM2n6J3mU4dxgKkwF30raJDOjS2bSCvXTm9Hhnny6XM6Pc889AFxiNMZZlt8tl4TQeIyPwBAzQqM0QckxGCJESJSnNB-AM-_XKOw4Y6dggDNM8yAMgZxwpaQ2Er5K43Wjf6SHz9YI_a2FNis4azfcwKkunS1r7hsPGws_4JJ3HpYdnBvRVjuOw5lefcKpk1-tNFUHrYJP2ju55dqdgxPFay8v9nME3qcPb5NZtHh5nE_uF1GZJKSJ4lhkNA_hVIw5S4TIE0YprpIqjSUOP1GuhEKCoFgiRpEoecqUEpkqGctxTkbgpvfdOhti-KbYaF_JuuZG2tYXGc1IHCwDeNuDlbM-hFTF1ukNd12BUbGrtOgrLXBAr_eebbmR4gDuOwzAVQ-sfWPdn05SitOUHTKV2loj_z_0C2rOhkM</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Kawata, Tetsuya</creator><creator>Ito, Hisao</creator><creator>Saito, Masayoshi</creator><creator>Uno, Takashi</creator><creator>Okayasu, Ryuichi</creator><creator>Liu, Cuihua</creator><creator>Kan'o, Momoe</creator><creator>George, Kerry</creator><creator>Cucinotta, Francis A.</creator><general>Radiation Research Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20051001</creationdate><title>Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair</title><author>Kawata, Tetsuya ; Ito, Hisao ; Saito, Masayoshi ; Uno, Takashi ; Okayasu, Ryuichi ; Liu, Cuihua ; Kan'o, Momoe ; George, Kerry ; Cucinotta, Francis A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b443t-22d687161f21a94dd749881c4c52e14048afdf0d302e0980dba59ffd6fb997173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Ataxia telangiectasia</topic><topic>Caffeine - pharmacology</topic><topic>CELL BIOLOGY</topic><topic>Cell cycle</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Chromosome Aberrations</topic><topic>Chromosomes</topic><topic>DNA damage</topic><topic>DNA Repair - drug effects</topic><topic>Fibroblasts</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - radiation effects</topic><topic>HeLa cells</topic><topic>Humans</topic><topic>Interphase</topic><topic>Irradiation</topic><topic>Lymphocytes</topic><topic>Radiation-Sensitizing Agents - pharmacology</topic><topic>Recombination, Genetic - drug effects</topic><topic>Space life sciences</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawata, Tetsuya</creatorcontrib><creatorcontrib>Ito, Hisao</creatorcontrib><creatorcontrib>Saito, Masayoshi</creatorcontrib><creatorcontrib>Uno, Takashi</creatorcontrib><creatorcontrib>Okayasu, Ryuichi</creatorcontrib><creatorcontrib>Liu, Cuihua</creatorcontrib><creatorcontrib>Kan'o, Momoe</creatorcontrib><creatorcontrib>George, Kerry</creatorcontrib><creatorcontrib>Cucinotta, Francis A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawata, Tetsuya</au><au>Ito, Hisao</au><au>Saito, Masayoshi</au><au>Uno, Takashi</au><au>Okayasu, Ryuichi</au><au>Liu, Cuihua</au><au>Kan'o, Momoe</au><au>George, Kerry</au><au>Cucinotta, Francis A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair</atitle><jtitle>Radiation research</jtitle><addtitle>Radiat Res</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>164</volume><issue>4</issue><spage>509</spage><epage>513</epage><pages>509-513</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><abstract>Kawata, T., Ito, H., Saito, M., Uno, T., Okayasu, R., Liu, C., Kan'o, M., George, K. and Cucinotta, F. A. Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair. Radiat. Res. 164, 509–513 (2005). Caffeine sensitizes cells to ionizing radiation, and this effect is believed to be associated with the disruption of DNA damage-responsive cell cycle checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining of DSBs is one of the underlying mechanisms of AT cell hyper-radiosensitivity. In this study, we investigated the effects of caffeine and radiation on nongrowing G0 normal human fibroblast cells by determining cell survival and scoring aberrations in calyculin A-induced G2 chromosomes. Results from the cell survival study indicate that after X-ray exposure G0 cells were sensitized by 24 h treatment with caffeine. Analysis of chromosome aberrations using FISH (fluorescence in situ hybridization) revealed a high frequency of aberrant cells and color junctions in the caffeine-treated cells. Since most DNA repair in nongrowing G0 cells is believed to result from nonhomologous end joining (NHEJ), caffeine may influence the fidelity of the NHEJ pathway in irradiated G0 cells.</abstract><cop>United States</cop><pub>Radiation Research Society</pub><pmid>16187758</pmid><doi>10.1667/RR3382.1</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0033-7587 |
| ispartof | Radiation research, 2005-10, Vol.164 (4), p.509-513 |
| issn | 0033-7587 1938-5404 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68632988 |
| source | MEDLINE; BioOne Complete; JSTOR Archive Collection A-Z Listing |
| subjects | Ataxia telangiectasia Caffeine - pharmacology CELL BIOLOGY Cell cycle Cell Line Cell lines Chromosome Aberrations Chromosomes DNA damage DNA Repair - drug effects Fibroblasts Fibroblasts - drug effects Fibroblasts - radiation effects HeLa cells Humans Interphase Irradiation Lymphocytes Radiation-Sensitizing Agents - pharmacology Recombination, Genetic - drug effects Space life sciences X-Rays |
| title | Caffeine Sensitizes Nondividing Human Fibroblasts to X Rays by Inducing a High Frequency of Misrepair |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T07%3A43%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Caffeine%20Sensitizes%20Nondividing%20Human%20Fibroblasts%20to%20X%20Rays%20by%20Inducing%20a%20High%20Frequency%20of%20Misrepair&rft.jtitle=Radiation%20research&rft.au=Kawata,%20Tetsuya&rft.date=2005-10-01&rft.volume=164&rft.issue=4&rft.spage=509&rft.epage=513&rft.pages=509-513&rft.issn=0033-7587&rft.eissn=1938-5404&rft_id=info:doi/10.1667/RR3382.1&rft_dat=%3Cjstor_proqu%3E3581559%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68632988&rft_id=info:pmid/16187758&rft_jstor_id=3581559&rfr_iscdi=true |