Fish is superior to PCR in detecting t(14;18)(q32;q21)-IgH/bcl-2 in follicular lymphoma using paraffin-embedded tissue samples
Detection of t(14;18)(q32;q21)-IgH/bcl-2, which is present in 70% to 95% of follicular lymphomas (FLs), might aid in diagnosing FL. The efficacy of routine polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques in detecting t(14;18) in paraffin-embedded tissue sampl...
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| Veröffentlicht in: | American journal of clinical pathology 2005-09, Vol.124 (3), p.421-429 |
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| container_title | American journal of clinical pathology |
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| creator | EINERSON, Richard R KURTIN, Paul J DAYHARSH, Gerald A KIMLINGER, Teresa K REMSTEIN, Ellen D |
| description | Detection of t(14;18)(q32;q21)-IgH/bcl-2, which is present in 70% to 95% of follicular lymphomas (FLs), might aid in diagnosing FL. The efficacy of routine polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques in detecting t(14;18) in paraffin-embedded tissue samples was compared on 5 normal tonsils and 28 FLs demonstrated to be t(14;18)+ by previous karyotyping. There was technical failure in 14 (50%) of the FLs by PCR, likely due to B-5 fixation, and 4 (14%) of FLs by FISH, likely due to advanced specimen age. In the remaining successful cases, 5 (36%) of 14 were positive by PCR and 24 (100%) of 24 were positive by FISH. All 5 normal tonsils were negative by both methods. FISH is superior to PCR for detecting t(14;18) from paraffin-embedded tissue samples because it is more sensitive and equally specific. |
| doi_str_mv | 10.1309/BLH8MMK85UBQ4K6R |
| format | Article |
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The efficacy of routine polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques in detecting t(14;18) in paraffin-embedded tissue samples was compared on 5 normal tonsils and 28 FLs demonstrated to be t(14;18)+ by previous karyotyping. There was technical failure in 14 (50%) of the FLs by PCR, likely due to B-5 fixation, and 4 (14%) of FLs by FISH, likely due to advanced specimen age. In the remaining successful cases, 5 (36%) of 14 were positive by PCR and 24 (100%) of 24 were positive by FISH. All 5 normal tonsils were negative by both methods. FISH is superior to PCR for detecting t(14;18) from paraffin-embedded tissue samples because it is more sensitive and equally specific.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1309/BLH8MMK85UBQ4K6R</identifier><identifier>PMID: 16191511</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Biological and medical sciences ; Chromosomes, Human, Pair 14 ; Chromosomes, Human, Pair 18 ; Gene Rearrangement ; Hematologic and hematopoietic diseases ; Humans ; Immunoglobulin Heavy Chains - genetics ; In Situ Hybridization, Fluorescence - methods ; Investigative techniques, diagnostic techniques (general aspects) ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Lymphoma, Follicular - genetics ; Medical sciences ; Paraffin Embedding ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Polymerase Chain Reaction - methods ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Translocation, Genetic</subject><ispartof>American journal of clinical pathology, 2005-09, Vol.124 (3), p.421-429</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-91d4fe8b6a9527f6e12284fb828d8a24e58610012a625310ff69cbc76a86fd703</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17170505$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16191511$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EINERSON, Richard R</creatorcontrib><creatorcontrib>KURTIN, Paul J</creatorcontrib><creatorcontrib>DAYHARSH, Gerald A</creatorcontrib><creatorcontrib>KIMLINGER, Teresa K</creatorcontrib><creatorcontrib>REMSTEIN, Ellen D</creatorcontrib><title>Fish is superior to PCR in detecting t(14;18)(q32;q21)-IgH/bcl-2 in follicular lymphoma using paraffin-embedded tissue samples</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>Detection of t(14;18)(q32;q21)-IgH/bcl-2, which is present in 70% to 95% of follicular lymphomas (FLs), might aid in diagnosing FL. The efficacy of routine polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques in detecting t(14;18) in paraffin-embedded tissue samples was compared on 5 normal tonsils and 28 FLs demonstrated to be t(14;18)+ by previous karyotyping. There was technical failure in 14 (50%) of the FLs by PCR, likely due to B-5 fixation, and 4 (14%) of FLs by FISH, likely due to advanced specimen age. In the remaining successful cases, 5 (36%) of 14 were positive by PCR and 24 (100%) of 24 were positive by FISH. All 5 normal tonsils were negative by both methods. FISH is superior to PCR for detecting t(14;18) from paraffin-embedded tissue samples because it is more sensitive and equally specific.</description><subject>Biological and medical sciences</subject><subject>Chromosomes, Human, Pair 14</subject><subject>Chromosomes, Human, Pair 18</subject><subject>Gene Rearrangement</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Immunoglobulin Heavy Chains - genetics</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Lymphoma, Follicular - genetics</subject><subject>Medical sciences</subject><subject>Paraffin Embedding</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Translocation, Genetic</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1vFDEURS0EIkugp0JuQEkxxM8ee2ylIivCRtkIiEg98njsxMjzsX4zRRp-O7PKSpFoqG5zzpWuLiHvgX0GwczZxXajb26utby7-Fleq9sXZAWmFEVVcf6SrBhjvDBQiSPyBvE3Y8A1K1-TI1BgQAKsyJ_LiA80IsV59DkOmU4D_bG-pbGnrZ-8m2J_T6cTKM9Bn57sBD_fcTgtru43Z41LBd-DYUgpujnZTNNjNz4MnaUz7sXRZhtC7AvfNb5tfUuniDh7irYbk8e35FWwCf27Qx6Tu8uvv9abYvv929X6y7ZwQupp2dCWwetGWSN5FZQHznUZGs11qy0vvdQK9vOs4lIAC0EZ17hKWa1CWzFxTD499Y552M0ep7qL6HxKtvfDjLXSSoBZ3P-BYCQozc0CsifQ5QEx-1CPOXY2P9bA6v059b_nLMqHQ_fcdL59Fg5vLMDHA2DR2RSy7V3EZ66CikkmxV8o_ZXy</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>EINERSON, Richard R</creator><creator>KURTIN, Paul J</creator><creator>DAYHARSH, Gerald A</creator><creator>KIMLINGER, Teresa K</creator><creator>REMSTEIN, Ellen D</creator><general>American Society of Clinical Pathologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20050901</creationdate><title>Fish is superior to PCR in detecting t(14;18)(q32;q21)-IgH/bcl-2 in follicular lymphoma using paraffin-embedded tissue samples</title><author>EINERSON, Richard R ; KURTIN, Paul J ; DAYHARSH, Gerald A ; KIMLINGER, Teresa K ; REMSTEIN, Ellen D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-91d4fe8b6a9527f6e12284fb828d8a24e58610012a625310ff69cbc76a86fd703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biological and medical sciences</topic><topic>Chromosomes, Human, Pair 14</topic><topic>Chromosomes, Human, Pair 18</topic><topic>Gene Rearrangement</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Immunoglobulin Heavy Chains - genetics</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Lymphoma, Follicular - genetics</topic><topic>Medical sciences</topic><topic>Paraffin Embedding</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Proto-Oncogene Proteins c-bcl-2 - genetics</topic><topic>Translocation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EINERSON, Richard R</creatorcontrib><creatorcontrib>KURTIN, Paul J</creatorcontrib><creatorcontrib>DAYHARSH, Gerald A</creatorcontrib><creatorcontrib>KIMLINGER, Teresa K</creatorcontrib><creatorcontrib>REMSTEIN, Ellen D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>EINERSON, Richard R</au><au>KURTIN, Paul J</au><au>DAYHARSH, Gerald A</au><au>KIMLINGER, Teresa K</au><au>REMSTEIN, Ellen D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fish is superior to PCR in detecting t(14;18)(q32;q21)-IgH/bcl-2 in follicular lymphoma using paraffin-embedded tissue samples</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>124</volume><issue>3</issue><spage>421</spage><epage>429</epage><pages>421-429</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><coden>AJCPAI</coden><abstract>Detection of t(14;18)(q32;q21)-IgH/bcl-2, which is present in 70% to 95% of follicular lymphomas (FLs), might aid in diagnosing FL. The efficacy of routine polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques in detecting t(14;18) in paraffin-embedded tissue samples was compared on 5 normal tonsils and 28 FLs demonstrated to be t(14;18)+ by previous karyotyping. There was technical failure in 14 (50%) of the FLs by PCR, likely due to B-5 fixation, and 4 (14%) of FLs by FISH, likely due to advanced specimen age. In the remaining successful cases, 5 (36%) of 14 were positive by PCR and 24 (100%) of 24 were positive by FISH. All 5 normal tonsils were negative by both methods. FISH is superior to PCR for detecting t(14;18) from paraffin-embedded tissue samples because it is more sensitive and equally specific.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>16191511</pmid><doi>10.1309/BLH8MMK85UBQ4K6R</doi><tpages>9</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biological and medical sciences Chromosomes, Human, Pair 14 Chromosomes, Human, Pair 18 Gene Rearrangement Hematologic and hematopoietic diseases Humans Immunoglobulin Heavy Chains - genetics In Situ Hybridization, Fluorescence - methods Investigative techniques, diagnostic techniques (general aspects) Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphoma, Follicular - genetics Medical sciences Paraffin Embedding Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction - methods Proto-Oncogene Proteins c-bcl-2 - genetics Translocation, Genetic |
| title | Fish is superior to PCR in detecting t(14;18)(q32;q21)-IgH/bcl-2 in follicular lymphoma using paraffin-embedded tissue samples |
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