Cholinomimetic and calcium channel blocking activities of Carthamus oxycantha
The crude extract of Carthamus oxycantha (Co.Cr) and its fractions were studied in vitro for their possible spasmogenic and spasmolytic activities. Co.Cr (0.03–10 mg/mL) caused an atropine sensitive spasmogenic effect in guinea‐pig ileum. In spontaneously contracting rabbit jejunum preparations, Co....
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| Veröffentlicht in: | Phytotherapy research 2005-08, Vol.19 (8), p.679-683 |
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| creator | Gilani, A.H Bukhari, I.A Khan, R.A Khan, A.U Ullah, F Ahmad, V.U |
| description | The crude extract of Carthamus oxycantha (Co.Cr) and its fractions were studied in vitro for their possible spasmogenic and spasmolytic activities. Co.Cr (0.03–10 mg/mL) caused an atropine sensitive spasmogenic effect in guinea‐pig ileum. In spontaneously contracting rabbit jejunum preparations, Co.Cr caused a dose‐dependent (0.03–3.0 mg/mL) spasmogenic effect, followed by relaxation at the next higher doses of 5.0–10.0 mg/mL. In the presence of atropine, the spasmogenic effect was blocked and the relaxant effect was observed at lower doses (0.1–5.0 mg/mL), shifting the inhibitory dose‐response curves to the left. Co.Cr also inhibited K+ (80 mm)‐induced contractions in atropinized preparations at similar doses, suggesting calcium channel blockade (CCB) activity. The CCB effect was further confirmed when pretreatment of the tissue with Co.Cr produced a dose‐dependent shift in the Ca++ dose‐response curves to the right, similar to that caused by verapamil. Activity‐directed fractionation revealed that the spasmolytic effect was concentrated in organic fractions in the following order of potency: hexane > ethylacetate > chloroform, while the aqueous fraction exhibited spasmogenic and weak spasmolytic effects. These results indicate that Carthamus oxycantha contains a combination of spasmogenic (cholinergic) and spasmolytic (calcium antagonist) constituents. Copyright © 2005 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/ptr.1727 |
| format | Article |
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Co.Cr (0.03–10 mg/mL) caused an atropine sensitive spasmogenic effect in guinea‐pig ileum. In spontaneously contracting rabbit jejunum preparations, Co.Cr caused a dose‐dependent (0.03–3.0 mg/mL) spasmogenic effect, followed by relaxation at the next higher doses of 5.0–10.0 mg/mL. In the presence of atropine, the spasmogenic effect was blocked and the relaxant effect was observed at lower doses (0.1–5.0 mg/mL), shifting the inhibitory dose‐response curves to the left. Co.Cr also inhibited K+ (80 mm)‐induced contractions in atropinized preparations at similar doses, suggesting calcium channel blockade (CCB) activity. The CCB effect was further confirmed when pretreatment of the tissue with Co.Cr produced a dose‐dependent shift in the Ca++ dose‐response curves to the right, similar to that caused by verapamil. Activity‐directed fractionation revealed that the spasmolytic effect was concentrated in organic fractions in the following order of potency: hexane > ethylacetate > chloroform, while the aqueous fraction exhibited spasmogenic and weak spasmolytic effects. These results indicate that Carthamus oxycantha contains a combination of spasmogenic (cholinergic) and spasmolytic (calcium antagonist) constituents. Copyright © 2005 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.1727</identifier><identifier>PMID: 16177970</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Biological and medical sciences ; Biomimetics ; calcium antagonist ; Calcium Channel Blockers - pharmacology ; Calcium Signaling ; Carthamus - chemistry ; Carthamus oxycantha ; cholinergic ; Dose-Response Relationship, Drug ; Female ; General pharmacology ; Guinea Pigs ; Ileum - drug effects ; Jejunum - drug effects ; Male ; Medical sciences ; Muscle Contraction - drug effects ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Rabbits ; spasmogenic ; spasmolytic</subject><ispartof>Phytotherapy research, 2005-08, Vol.19 (8), p.679-683</ispartof><rights>Copyright © 2005 John Wiley & Sons, Ltd.</rights><rights>2005 INIST-CNRS</rights><rights>Copyright (c) 2005 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5087-5fc2152e394b586f5fc171c769e046de8c759df8c1d9479ed31103e73d05b03c3</citedby><cites>FETCH-LOGICAL-c5087-5fc2152e394b586f5fc171c769e046de8c759df8c1d9479ed31103e73d05b03c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fptr.1727$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fptr.1727$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17128329$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16177970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gilani, A.H</creatorcontrib><creatorcontrib>Bukhari, I.A</creatorcontrib><creatorcontrib>Khan, R.A</creatorcontrib><creatorcontrib>Khan, A.U</creatorcontrib><creatorcontrib>Ullah, F</creatorcontrib><creatorcontrib>Ahmad, V.U</creatorcontrib><title>Cholinomimetic and calcium channel blocking activities of Carthamus oxycantha</title><title>Phytotherapy research</title><addtitle>Phytother. Res</addtitle><description>The crude extract of Carthamus oxycantha (Co.Cr) and its fractions were studied in vitro for their possible spasmogenic and spasmolytic activities. Co.Cr (0.03–10 mg/mL) caused an atropine sensitive spasmogenic effect in guinea‐pig ileum. In spontaneously contracting rabbit jejunum preparations, Co.Cr caused a dose‐dependent (0.03–3.0 mg/mL) spasmogenic effect, followed by relaxation at the next higher doses of 5.0–10.0 mg/mL. In the presence of atropine, the spasmogenic effect was blocked and the relaxant effect was observed at lower doses (0.1–5.0 mg/mL), shifting the inhibitory dose‐response curves to the left. Co.Cr also inhibited K+ (80 mm)‐induced contractions in atropinized preparations at similar doses, suggesting calcium channel blockade (CCB) activity. The CCB effect was further confirmed when pretreatment of the tissue with Co.Cr produced a dose‐dependent shift in the Ca++ dose‐response curves to the right, similar to that caused by verapamil. Activity‐directed fractionation revealed that the spasmolytic effect was concentrated in organic fractions in the following order of potency: hexane > ethylacetate > chloroform, while the aqueous fraction exhibited spasmogenic and weak spasmolytic effects. These results indicate that Carthamus oxycantha contains a combination of spasmogenic (cholinergic) and spasmolytic (calcium antagonist) constituents. Copyright © 2005 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomimetics</subject><subject>calcium antagonist</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium Signaling</subject><subject>Carthamus - chemistry</subject><subject>Carthamus oxycantha</subject><subject>cholinergic</subject><subject>Dose-Response Relationship, Drug</subject><subject>Female</subject><subject>General pharmacology</subject><subject>Guinea Pigs</subject><subject>Ileum - drug effects</subject><subject>Jejunum - drug effects</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Muscle Contraction - drug effects</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Rabbits</subject><subject>spasmogenic</subject><subject>spasmolytic</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0k1P3DAQBmCragVbisQvgFyoegmdsePYPqKoSyvRD1FQe7O8jsMa8rHYScv--xptVE5VT9ZIj2c8r0zIEcIZAtD3mzGcoaDiBVkgKJUjF-wlWYDimBcof-6T1zHeAYCiUOyRfSxRCCVgQT5X66H1_dD5zo3eZqavM2ta66cus2vT967NVu1g731_mxk7-l9-9C5mQ5NVJoxr002peNxa06fiDXnVmDa6w_k8IDfLD9fVx_zy68Wn6vwytxykyHljKXLqmCpWXJZNqlGgFaVyUJS1k1ZwVTfSYq0KoVzNEIE5wWrgK2CWHZC3u76bMDxMLo6689G6tjW9G6aoS1lSKQr5X4iCCSqRJfhuB20YYgyu0ZvgOxO2GkE_ZaxTxvop40SP557TqnP1M5xDTeB0BiamLJtgeuvjsxNIJaMquXznfvvWbf85UH-7vpoHz97H0T3-9Sbc6zLtwfWPLxfpRimXFVvqq-RPdr4xgza3Ib3h5jsFZJDSLHj6I38AqtyqNw</recordid><startdate>200508</startdate><enddate>200508</enddate><creator>Gilani, A.H</creator><creator>Bukhari, I.A</creator><creator>Khan, R.A</creator><creator>Khan, A.U</creator><creator>Ullah, F</creator><creator>Ahmad, V.U</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>200508</creationdate><title>Cholinomimetic and calcium channel blocking activities of Carthamus oxycantha</title><author>Gilani, A.H ; Bukhari, I.A ; Khan, R.A ; Khan, A.U ; Ullah, F ; Ahmad, V.U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5087-5fc2152e394b586f5fc171c769e046de8c759df8c1d9479ed31103e73d05b03c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomimetics</topic><topic>calcium antagonist</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium Signaling</topic><topic>Carthamus - chemistry</topic><topic>Carthamus oxycantha</topic><topic>cholinergic</topic><topic>Dose-Response Relationship, Drug</topic><topic>Female</topic><topic>General pharmacology</topic><topic>Guinea Pigs</topic><topic>Ileum - drug effects</topic><topic>Jejunum - drug effects</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Muscle Contraction - drug effects</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Rabbits</topic><topic>spasmogenic</topic><topic>spasmolytic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gilani, A.H</creatorcontrib><creatorcontrib>Bukhari, I.A</creatorcontrib><creatorcontrib>Khan, R.A</creatorcontrib><creatorcontrib>Khan, A.U</creatorcontrib><creatorcontrib>Ullah, F</creatorcontrib><creatorcontrib>Ahmad, V.U</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gilani, A.H</au><au>Bukhari, I.A</au><au>Khan, R.A</au><au>Khan, A.U</au><au>Ullah, F</au><au>Ahmad, V.U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cholinomimetic and calcium channel blocking activities of Carthamus oxycantha</atitle><jtitle>Phytotherapy research</jtitle><addtitle>Phytother. Res</addtitle><date>2005-08</date><risdate>2005</risdate><volume>19</volume><issue>8</issue><spage>679</spage><epage>683</epage><pages>679-683</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><abstract>The crude extract of Carthamus oxycantha (Co.Cr) and its fractions were studied in vitro for their possible spasmogenic and spasmolytic activities. Co.Cr (0.03–10 mg/mL) caused an atropine sensitive spasmogenic effect in guinea‐pig ileum. In spontaneously contracting rabbit jejunum preparations, Co.Cr caused a dose‐dependent (0.03–3.0 mg/mL) spasmogenic effect, followed by relaxation at the next higher doses of 5.0–10.0 mg/mL. In the presence of atropine, the spasmogenic effect was blocked and the relaxant effect was observed at lower doses (0.1–5.0 mg/mL), shifting the inhibitory dose‐response curves to the left. Co.Cr also inhibited K+ (80 mm)‐induced contractions in atropinized preparations at similar doses, suggesting calcium channel blockade (CCB) activity. The CCB effect was further confirmed when pretreatment of the tissue with Co.Cr produced a dose‐dependent shift in the Ca++ dose‐response curves to the right, similar to that caused by verapamil. Activity‐directed fractionation revealed that the spasmolytic effect was concentrated in organic fractions in the following order of potency: hexane > ethylacetate > chloroform, while the aqueous fraction exhibited spasmogenic and weak spasmolytic effects. These results indicate that Carthamus oxycantha contains a combination of spasmogenic (cholinergic) and spasmolytic (calcium antagonist) constituents. Copyright © 2005 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>16177970</pmid><doi>10.1002/ptr.1727</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Biomimetics calcium antagonist Calcium Channel Blockers - pharmacology Calcium Signaling Carthamus - chemistry Carthamus oxycantha cholinergic Dose-Response Relationship, Drug Female General pharmacology Guinea Pigs Ileum - drug effects Jejunum - drug effects Male Medical sciences Muscle Contraction - drug effects Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - chemistry Plant Extracts - pharmacology Rabbits spasmogenic spasmolytic |
| title | Cholinomimetic and calcium channel blocking activities of Carthamus oxycantha |
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