Localization of actin filaments on mitotic apparatus in tobacco BY-2 cells
Actin filaments are among the major components of the cytoskeleton, and participate in various cellular dynamic processes. However, conflicting results had been obtained on the localization of actin filaments on the mitotic apparatus and their participation in the process of chromosome segregation....
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| Veröffentlicht in: | Planta 2005-09, Vol.222 (1), p.118-129 |
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| description | Actin filaments are among the major components of the cytoskeleton, and participate in various cellular dynamic processes. However, conflicting results had been obtained on the localization of actin filaments on the mitotic apparatus and their participation in the process of chromosome segregation. We demonstrated by using rhodamine-phalloidin staining, the localization of actin filaments on the mitotic spindles of tobacco BY-2 cells when the cells were treated with cytochalasin D. At prophase, several clear spots were observed at or near the kinetochores of the chromosomes. At anaphase, the actin filaments that appeared to be pulling chromosomes toward the division poles were demonstrated. However, as there was a slight possibility that these results might have been the artifacts of cytochalasin D treatment or the phalloidin staining, we analyzed the localization of actin filaments at the mitotic apparatus immunologically. We cloned a novel BY-2 alpha-type actin cDNA and prepared a BY-2 actin antibody. The fluorescence of the anti-BY-2 actin antibody was clearly observed at the mitotic apparatus in both non-treated and cytochalasin D-treated BY-2 cells during mitosis. The facts that similar results were obtained in both actin staining with rhodamine-phalloidin and immunostaining with actin antibody strongly indicate the participation of actin in the organization of the spindle body or in the process of chromosome segregation. Furthermore, both filamentous actin and spindle bodies disappeared in the cells treated with propyzamide, which depolymerizes microtubules, supporting the notion that actin filaments are associated with microtubules organizing the spindle body. |
| doi_str_mv | 10.1007/s00425-005-1522-8 |
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However, conflicting results had been obtained on the localization of actin filaments on the mitotic apparatus and their participation in the process of chromosome segregation. We demonstrated by using rhodamine-phalloidin staining, the localization of actin filaments on the mitotic spindles of tobacco BY-2 cells when the cells were treated with cytochalasin D. At prophase, several clear spots were observed at or near the kinetochores of the chromosomes. At anaphase, the actin filaments that appeared to be pulling chromosomes toward the division poles were demonstrated. However, as there was a slight possibility that these results might have been the artifacts of cytochalasin D treatment or the phalloidin staining, we analyzed the localization of actin filaments at the mitotic apparatus immunologically. We cloned a novel BY-2 alpha-type actin cDNA and prepared a BY-2 actin antibody. The fluorescence of the anti-BY-2 actin antibody was clearly observed at the mitotic apparatus in both non-treated and cytochalasin D-treated BY-2 cells during mitosis. The facts that similar results were obtained in both actin staining with rhodamine-phalloidin and immunostaining with actin antibody strongly indicate the participation of actin in the organization of the spindle body or in the process of chromosome segregation. Furthermore, both filamentous actin and spindle bodies disappeared in the cells treated with propyzamide, which depolymerizes microtubules, supporting the notion that actin filaments are associated with microtubules organizing the spindle body.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-005-1522-8</identifier><identifier>PMID: 15856282</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>actin ; Actin Cytoskeleton - chemistry ; Actin Cytoskeleton - drug effects ; Actin Cytoskeleton - metabolism ; Actins ; Actins - chemistry ; Actins - genetics ; Actins - immunology ; Actins - metabolism ; Amino Acid Sequence ; amino acid sequences ; Animals ; Antibodies ; Benzamides - pharmacology ; Biological and medical sciences ; Cell kinetics ; Cell physiology ; cell suspension culture ; Cells, Cultured ; chromosome segregation ; Chromosomes ; cultured cells ; cytochalasin D ; Cytochalasin D - pharmacology ; Cytochalasins ; Cytoskeleton ; Fundamental and applied biological sciences. Psychology ; HeLa cells ; Humans ; Microfilaments ; Microtubules ; Mitosis ; Mitotic spindle apparatus ; Molecular Sequence Data ; Nicotiana - cytology ; Nicotiana tabacum ; nucleotide sequences ; Phylogeny ; plant biochemistry ; Plant cells ; plant physiology ; Plant physiology and development ; propyzamide ; Proteins ; Sequence Homology, Amino Acid ; Spindle Apparatus - drug effects ; Spindle Apparatus - metabolism ; tobacco</subject><ispartof>Planta, 2005-09, Vol.222 (1), p.118-129</ispartof><rights>Springer-Verlag Berlin Heidelberg 2005</rights><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-da77f698551669f18b3b95409c53121c03b50008db28a968d00e8c787efdb0303</citedby><cites>FETCH-LOGICAL-c402t-da77f698551669f18b3b95409c53121c03b50008db28a968d00e8c787efdb0303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23388966$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23388966$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17104559$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15856282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yasuda, H</creatorcontrib><creatorcontrib>Kanda, K</creatorcontrib><creatorcontrib>Suenaga, K</creatorcontrib><creatorcontrib>Kidou, S</creatorcontrib><creatorcontrib>Ejiri, S</creatorcontrib><title>Localization of actin filaments on mitotic apparatus in tobacco BY-2 cells</title><title>Planta</title><addtitle>Planta</addtitle><description>Actin filaments are among the major components of the cytoskeleton, and participate in various cellular dynamic processes. However, conflicting results had been obtained on the localization of actin filaments on the mitotic apparatus and their participation in the process of chromosome segregation. We demonstrated by using rhodamine-phalloidin staining, the localization of actin filaments on the mitotic spindles of tobacco BY-2 cells when the cells were treated with cytochalasin D. At prophase, several clear spots were observed at or near the kinetochores of the chromosomes. At anaphase, the actin filaments that appeared to be pulling chromosomes toward the division poles were demonstrated. However, as there was a slight possibility that these results might have been the artifacts of cytochalasin D treatment or the phalloidin staining, we analyzed the localization of actin filaments at the mitotic apparatus immunologically. We cloned a novel BY-2 alpha-type actin cDNA and prepared a BY-2 actin antibody. The fluorescence of the anti-BY-2 actin antibody was clearly observed at the mitotic apparatus in both non-treated and cytochalasin D-treated BY-2 cells during mitosis. The facts that similar results were obtained in both actin staining with rhodamine-phalloidin and immunostaining with actin antibody strongly indicate the participation of actin in the organization of the spindle body or in the process of chromosome segregation. Furthermore, both filamentous actin and spindle bodies disappeared in the cells treated with propyzamide, which depolymerizes microtubules, supporting the notion that actin filaments are associated with microtubules organizing the spindle body.</description><subject>actin</subject><subject>Actin Cytoskeleton - chemistry</subject><subject>Actin Cytoskeleton - drug effects</subject><subject>Actin Cytoskeleton - metabolism</subject><subject>Actins</subject><subject>Actins - chemistry</subject><subject>Actins - genetics</subject><subject>Actins - immunology</subject><subject>Actins - metabolism</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Benzamides - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell kinetics</subject><subject>Cell physiology</subject><subject>cell suspension culture</subject><subject>Cells, Cultured</subject><subject>chromosome segregation</subject><subject>Chromosomes</subject><subject>cultured cells</subject><subject>cytochalasin D</subject><subject>Cytochalasin D - pharmacology</subject><subject>Cytochalasins</subject><subject>Cytoskeleton</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa cells</subject><subject>Humans</subject><subject>Microfilaments</subject><subject>Microtubules</subject><subject>Mitosis</subject><subject>Mitotic spindle apparatus</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - cytology</subject><subject>Nicotiana tabacum</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>plant biochemistry</subject><subject>Plant cells</subject><subject>plant physiology</subject><subject>Plant physiology and development</subject><subject>propyzamide</subject><subject>Proteins</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spindle Apparatus - drug effects</subject><subject>Spindle Apparatus - metabolism</subject><subject>tobacco</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkE2r1DAUhoMo3nH0B7hQi6C76knSJKfL68VPBlzoXbgKaZpIhrYZk3Shv94MHbwgBBLyPufl8BDylMIbCqDeZoCOiRZAtFQw1uI9sqMdZy2DDu-THUB9Q8_FFXmU8xGghko9JFdUoJAM2Y58OURrpvDHlBCXJvrG2BKWxofJzG4puam_cyixBNuY08kkU9bcVKLEwVgbm3c_WtZYN035MXngzZTdk8u9J7cf3n-_-dQevn78fHN9aG0HrLSjUcrLHoWgUvae4sCHXnTQW8Epoxb4IAAAx4Gh6SWOAA6tQuX8OAAHvievt95Tir9Wl4ueQz5vYBYX16wlSqZ4PXvy8j_wGNe01N00MkAExrsK0Q2yKeacnNenFGaTfmsK-mxZb5Z1tazPljXWmeeX4nWY3Xg3cdFagVcXwOSq1yez2JDvOEWhE6Kv3LONO-YS07-ccY7YS1nzF1vuTdTmZ6odt98YUA4UpISO87_2OJS7</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>Yasuda, H</creator><creator>Kanda, K</creator><creator>Suenaga, K</creator><creator>Kidou, S</creator><creator>Ejiri, S</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050901</creationdate><title>Localization of actin filaments on mitotic apparatus in tobacco BY-2 cells</title><author>Yasuda, H ; Kanda, K ; Suenaga, K ; Kidou, S ; Ejiri, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-da77f698551669f18b3b95409c53121c03b50008db28a968d00e8c787efdb0303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>actin</topic><topic>Actin Cytoskeleton - chemistry</topic><topic>Actin Cytoskeleton - drug effects</topic><topic>Actin Cytoskeleton - metabolism</topic><topic>Actins</topic><topic>Actins - chemistry</topic><topic>Actins - genetics</topic><topic>Actins - immunology</topic><topic>Actins - metabolism</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Benzamides - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell kinetics</topic><topic>Cell physiology</topic><topic>cell suspension culture</topic><topic>Cells, Cultured</topic><topic>chromosome segregation</topic><topic>Chromosomes</topic><topic>cultured cells</topic><topic>cytochalasin D</topic><topic>Cytochalasin D - pharmacology</topic><topic>Cytochalasins</topic><topic>Cytoskeleton</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa cells</topic><topic>Humans</topic><topic>Microfilaments</topic><topic>Microtubules</topic><topic>Mitosis</topic><topic>Mitotic spindle apparatus</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - cytology</topic><topic>Nicotiana tabacum</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>plant biochemistry</topic><topic>Plant cells</topic><topic>plant physiology</topic><topic>Plant physiology and development</topic><topic>propyzamide</topic><topic>Proteins</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spindle Apparatus - drug effects</topic><topic>Spindle Apparatus - metabolism</topic><topic>tobacco</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yasuda, H</creatorcontrib><creatorcontrib>Kanda, K</creatorcontrib><creatorcontrib>Suenaga, K</creatorcontrib><creatorcontrib>Kidou, S</creatorcontrib><creatorcontrib>Ejiri, S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yasuda, H</au><au>Kanda, K</au><au>Suenaga, K</au><au>Kidou, S</au><au>Ejiri, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of actin filaments on mitotic apparatus in tobacco BY-2 cells</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>222</volume><issue>1</issue><spage>118</spage><epage>129</epage><pages>118-129</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Actin filaments are among the major components of the cytoskeleton, and participate in various cellular dynamic processes. However, conflicting results had been obtained on the localization of actin filaments on the mitotic apparatus and their participation in the process of chromosome segregation. We demonstrated by using rhodamine-phalloidin staining, the localization of actin filaments on the mitotic spindles of tobacco BY-2 cells when the cells were treated with cytochalasin D. At prophase, several clear spots were observed at or near the kinetochores of the chromosomes. At anaphase, the actin filaments that appeared to be pulling chromosomes toward the division poles were demonstrated. However, as there was a slight possibility that these results might have been the artifacts of cytochalasin D treatment or the phalloidin staining, we analyzed the localization of actin filaments at the mitotic apparatus immunologically. We cloned a novel BY-2 alpha-type actin cDNA and prepared a BY-2 actin antibody. The fluorescence of the anti-BY-2 actin antibody was clearly observed at the mitotic apparatus in both non-treated and cytochalasin D-treated BY-2 cells during mitosis. The facts that similar results were obtained in both actin staining with rhodamine-phalloidin and immunostaining with actin antibody strongly indicate the participation of actin in the organization of the spindle body or in the process of chromosome segregation. Furthermore, both filamentous actin and spindle bodies disappeared in the cells treated with propyzamide, which depolymerizes microtubules, supporting the notion that actin filaments are associated with microtubules organizing the spindle body.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>15856282</pmid><doi>10.1007/s00425-005-1522-8</doi><tpages>12</tpages></addata></record> |
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| subjects | actin Actin Cytoskeleton - chemistry Actin Cytoskeleton - drug effects Actin Cytoskeleton - metabolism Actins Actins - chemistry Actins - genetics Actins - immunology Actins - metabolism Amino Acid Sequence amino acid sequences Animals Antibodies Benzamides - pharmacology Biological and medical sciences Cell kinetics Cell physiology cell suspension culture Cells, Cultured chromosome segregation Chromosomes cultured cells cytochalasin D Cytochalasin D - pharmacology Cytochalasins Cytoskeleton Fundamental and applied biological sciences. Psychology HeLa cells Humans Microfilaments Microtubules Mitosis Mitotic spindle apparatus Molecular Sequence Data Nicotiana - cytology Nicotiana tabacum nucleotide sequences Phylogeny plant biochemistry Plant cells plant physiology Plant physiology and development propyzamide Proteins Sequence Homology, Amino Acid Spindle Apparatus - drug effects Spindle Apparatus - metabolism tobacco |
| title | Localization of actin filaments on mitotic apparatus in tobacco BY-2 cells |
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